Reviewed, UniProtKB/Swiss-Prot Q9QXN1 (LEF1_RAT)

Names and origin

Protein names

Recommended name:
    Lymphoid enhancer-binding factor 1
      Short name=LEF-1

Gene names

Name:

Lef1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 By similarity.
Subunit structure	Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with TLE1, PIASG, THOC4, EP300, MDFI and MDFIC By similarity.
Subcellular location	Nucleus By similarity. Note: Found in nuclear bodies upon PIASG binding By similarity.
Domain	Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
Sequence similarities	Belongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Wnt signaling pathway
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation Isopeptide bond Ubl conjugation
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell adhesion Inferred from expression pattern. Source: RGD kidney development Inferred from expression pattern. Source: RGD response to lithium ion Inferred from expression pattern. Source: RGD response to organic cyclic substance Inferred from expression pattern. Source: RGD skin development Inferred from expression pattern. Source: RGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA bending activity Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Lymphoid enhancer-binding factor 1		PRO_0000048597
Regions
	DNA binding	297 – 365	69	HMG box
	Region	1 – 60	60	CTNNB1-binding By similarity
	Compositional bias	6 – 11	6	Poly-Gly
	Compositional bias	12 – 50	39	Asp/Glu-rich (acidic)
	Compositional bias	75 – 271	197	Pro-rich
	Compositional bias	372 – 377	6	Poly-Lys
Amino acid modifications
	Modified residue	93	1	N6-acetyllysine By similarity
	Cross-link	25		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	267		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QXN1-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D6BEFF805CB526EA	FASTA	397	44,023
	10 20 30 40 50 60 MPQLSGGGGG GDPELCATDE MIPFKDEGDP QKEKIFAEIS HPEEEGDLAD IKSSLVNESE 70 80 90 100 110 120 IIPASNGHEV VGQTQSSQEP YHDKAREHPD DGKHPDGGLY NKGPSYSSYS GYIMMPNMNS 130 140 150 160 170 180 DPYMSNGSLS PPIPRTSNKV PVVQPSHAVH PLTPLITYSD EHFSPGSHPS HIPSEVNPKQ 190 200 210 220 230 240 GMSRHPPAPE MPTFYPLSPG GVGQITPPLG WQGQPVYPIT GGFRQAYPSS LSGDTSMSRF 250 260 270 280 290 300 SHHMIPGPPG PHTTGIPHPA IVTPQVKQEH PHTDSDLMHV KPEHEQRKEQ EPKRPHIKKP 310 320 330 340 350 360 LNAFMLYMKE MRANVVAECT LKESAAINQI LGRRWHALSR EEQAKYYELA RKERQLHMQL 370 380 390 YPGWSARDNY GKKKKRKREK LQESTSGTGP RMTAAYI

References

[1]	"Cloning of the lymphoid enhancer binding factor-1 (Lef-1) cDNA from rat kidney: homology to the mouse sequence." Kobielak K., Kobielak A., Trzeciak W.H. Acta Biochim. Pol. 46:885-888(1999) [PubMed: 10824856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF198533 mRNA. Translation: AAF15601.1.
IPI	IPI00213373.
RefSeq	NP_569113.1.
UniGene	Rn.21926
3D structure databases
HSSP	HSSP built from PDB template 2LEF based on UniProtKB P27782.
SMR	Q9QXN1. Positions 296-371.
DisProt	DP00046.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9QXN1.
Genome annotation databases
Ensembl	ENSRNOT00000013694; ENSRNOP00000013694; ENSRNOG00000010121; Rattus norvegicus. [Genome view]
GeneID	161452.
KEGG	rno:161452.
Organism-specific databases
CTD	161452.
RGD	620241. Lef1.
Phylogenomic databases
HOVERGEN	Q9QXN1.
Gene expression databases
ArrayExpress	Q9QXN1.
Genevestigator	Q9QXN1.
GermOnline	ENSRNOG00000010121. Rattus norvegicus.
Family and domain databases
InterPro	IPR013558. CTNNB1_bd_N. IPR000910. HMG_HMG1/HMG2. [Graphical view]
Gene3D	G3DSA:1.10.30.10. HMG-box. 1 hit.
Pfam	PF08347. CTNNB1_binding. 1 hit. PF00505. HMG_box. 1 hit. [Graphical view]
SMART	SM00398. HMG. 1 hit. [Graphical view]
PROSITE	PS50118. HMG_BOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	620971.

Entry information

Entry name

LEF1_RAT

Accession

Primary (citable) accession number: Q9QXN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	October 13, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)