Q9QXN1 (LEF1_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 91.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Lymphoid enhancer-binding factor 1 Short name=LEF-1 | ||
| Gene names |
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| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 397 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 By similarity. |
| Subunit structure | Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with TLE1, PIASG, THOC4, EP300, MDFI and MDFIC By similarity. |
| Subcellular location | Nucleus By similarity. Note: Found in nuclear bodies upon PIASG binding By similarity. |
| Domain | Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. |
| Post-translational modification | Phosphorylated at Thr-153 and/or Ser-164 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway By similarity. |
| Sequence similarities | Belongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 397 | 397 | Lymphoid enhancer-binding factor 1 | PRO_0000048597 | |||||
Regions | |||||||||
| DNA binding | 297 – 365 | 69 | HMG box | ||||||
| Region | 1 – 60 | 60 | CTNNB1-binding By similarity | ||||||
| Compositional bias | 6 – 11 | 6 | Poly-Gly | ||||||
| Compositional bias | 12 – 50 | 39 | Asp/Glu-rich (acidic) | ||||||
| Compositional bias | 75 – 271 | 197 | Pro-rich | ||||||
| Compositional bias | 372 – 377 | 6 | Poly-Lys | ||||||
Amino acid modifications | |||||||||
| Modified residue | 93 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 130 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 153 | 1 | Phosphothreonine; by NLK By similarity | ||||||
| Modified residue | 164 | 1 | Phosphoserine; by NLK By similarity | ||||||
| Cross-link | 25 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity | |||||||
| Cross-link | 267 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity | |||||||
Sequences
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References
| [1] | "Cloning of the lymphoid enhancer binding factor-1 (Lef-1) cDNA from rat kidney: homology to the mouse sequence." Kobielak K., Kobielak A., Trzeciak W.H. Acta Biochim. Pol. 46:885-888(1999) [PubMed: 10824856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF198533 mRNA. Translation: AAF15601.1. |
| IPI | IPI00213373. |
| RefSeq | NP_569113.1. NM_130429.1. |
| UniGene | Rn.21926. |
3D structure databases | |
| ProteinModelPortal | Q9QXN1. |
| SMR | Q9QXN1. Positions 2-59, 296-371. |
| DisProt | DP00046. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q9QXN1. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 161452. |
| KEGG | rno:161452. |
Organism-specific databases | |
| CTD | 51176. |
| RGD | 620241. Lef1. |
Phylogenomic databases | |
| GeneTree | ENSGT00390000009964. |
| HOVERGEN | HBG000419. |
| InParanoid | Q9QXN1. |
Gene expression databases | |
| ArrayExpress | Q9QXN1. |
| Genevestigator | Q9QXN1. |
| GermOnline | ENSRNOG00000010121. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR013558. CTNNB1-bd_N. IPR000910. HMG_HMG1/HMG2. IPR009071. HMG_superfamily. [Graphical view] |
| Gene3D | G3DSA:1.10.30.10. HMG-box. 1 hit. |
| KO | K04492. |
| Pfam | PF08347. CTNNB1_binding. 1 hit. PF00505. HMG_box. 1 hit. [Graphical view] |
| SMART | SM00398. HMG. 1 hit. [Graphical view] |
| SUPFAM | SSF47095. HMG-box. 1 hit. |
| PROSITE | PS50118. HMG_BOX_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 620971. |
Entry information
| Entry name | LEF1_RAT | ||||||||
| Accession | Primary (citable) accession number: Q9QXN1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

Clusters with