Q9QXN1 (LEF1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lymphoid enhancer-binding factor 1
Short name=LEF-1

Gene names

Name:

Lef1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 By similarity.
Subunit structure	Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with TLE1, PIASG, THOC4, EP300, MDFI and MDFIC By similarity.
Subcellular location	Nucleus By similarity. Note: Found in nuclear bodies upon PIASG binding By similarity.
Domain	Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
Post-translational modification	Phosphorylated at Thr-153 and/or Ser-164 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway By similarity.
Sequence similarities	Belongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Wnt signaling pathway
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	B cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome BMP signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome alpha-beta T cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome apoptosis involved in morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome chorio-allantoic fusion Inferred from Biological aspect of Ancestor. Source: RefGenome dentate gyrus development Inferred from Biological aspect of Ancestor. Source: RefGenome embryonic limb morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome epithelial to mesenchymal transition Inferred from Biological aspect of Ancestor. Source: RefGenome eye pigmentation Inferred from Biological aspect of Ancestor. Source: RefGenome face morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome forebrain neuroblast division Inferred from Biological aspect of Ancestor. Source: RefGenome forebrain neuron differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome forebrain radial glial cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome formation of radial glial scaffolds Inferred from Biological aspect of Ancestor. Source: RefGenome hypothalamus development Inferred from Biological aspect of Ancestor. Source: RefGenome induction of apoptosis Inferred from Biological aspect of Ancestor. Source: RefGenome kidney development Inferred from expression pattern. Source: RGD mammary gland development Inferred from Biological aspect of Ancestor. Source: RefGenome muscle fiber development Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of apoptotic process in bone marrow Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of interleukin-13 production Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of interleukin-4 production Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of interleukin-5 production Inferred from Biological aspect of Ancestor. Source: RefGenome neural crest cell migration Inferred from Biological aspect of Ancestor. Source: RefGenome neutrophil differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome odontoblast differentiation Inferred from mutant phenotype. Source: RGD organ regeneration Inferred from expression pattern. Source: RGD osteoblast differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome paraxial mesoderm formation Inferred from Biological aspect of Ancestor. Source: RefGenome patterning of blood vessels Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of cell proliferation in bone marrow Inferred from Biological aspect of Ancestor. Source: RefGenome response to lithium ion Inferred from expression pattern. Source: RGD response to organic cyclic compound Inferred from expression pattern. Source: RGD skin development Inferred from expression pattern. Source: RGD somitogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome sprouting angiogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome trachea gland development Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular component	cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome transcription factor complex Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular function	DNA binding, bending Inferred from sequence or structural similarity. Source: UniProtKB armadillo repeat domain binding Inferred from Biological aspect of Ancestor. Source: RefGenome beta-catenin binding Inferred from Biological aspect of Ancestor. Source: RefGenome chromatin binding Inferred from Biological aspect of Ancestor. Source: RefGenome enhancer binding Inferred from Biological aspect of Ancestor. Source: RefGenome estrogen receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome gamma-catenin binding Inferred from Biological aspect of Ancestor. Source: RefGenome sequence-specific DNA binding Inferred from Biological aspect of Ancestor. Source: RefGenome sequence-specific distal enhancer binding RNA polymerase II transcription factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Lymphoid enhancer-binding factor 1		PRO_0000048597
Regions
	DNA binding	297 – 365	69	HMG box
	Region	1 – 60	60	CTNNB1-binding By similarity
	Compositional bias	6 – 11	6	Poly-Gly
	Compositional bias	12 – 50	39	Asp/Glu-rich (acidic)
	Compositional bias	75 – 271	197	Pro-rich
	Compositional bias	372 – 377	6	Poly-Lys
Amino acid modifications
	Modified residue	93	1	N6-acetyllysine By similarity
	Modified residue	130	1	Phosphoserine By similarity
	Modified residue	153	1	Phosphothreonine; by NLK By similarity
	Modified residue	164	1	Phosphoserine; by NLK By similarity
	Cross-link	25		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	267		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QXN1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D6BEFF805CB526EA	FASTA	397	44,023
	10 20 30 40 50 60 MPQLSGGGGG GDPELCATDE MIPFKDEGDP QKEKIFAEIS HPEEEGDLAD IKSSLVNESE 70 80 90 100 110 120 IIPASNGHEV VGQTQSSQEP YHDKAREHPD DGKHPDGGLY NKGPSYSSYS GYIMMPNMNS 130 140 150 160 170 180 DPYMSNGSLS PPIPRTSNKV PVVQPSHAVH PLTPLITYSD EHFSPGSHPS HIPSEVNPKQ 190 200 210 220 230 240 GMSRHPPAPE MPTFYPLSPG GVGQITPPLG WQGQPVYPIT GGFRQAYPSS LSGDTSMSRF 250 260 270 280 290 300 SHHMIPGPPG PHTTGIPHPA IVTPQVKQEH PHTDSDLMHV KPEHEQRKEQ EPKRPHIKKP 310 320 330 340 350 360 LNAFMLYMKE MRANVVAECT LKESAAINQI LGRRWHALSR EEQAKYYELA RKERQLHMQL 370 380 390 YPGWSARDNY GKKKKRKREK LQESTSGTGP RMTAAYI

References

[1]	"Cloning of the lymphoid enhancer binding factor-1 (Lef-1) cDNA from rat kidney: homology to the mouse sequence." Kobielak K., Kobielak A., Trzeciak W.H. Acta Biochim. Pol. 46:885-888(1999) [PubMed: 10824856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF198533 mRNA. Translation: AAF15601.1.
IPI	IPI00213373.
RefSeq	NP_569113.1. NM_130429.1.
UniGene	Rn.21926.
3D structure databases
ProteinModelPortal	Q9QXN1.
SMR	Q9QXN1. Positions 2-59, 296-371.
DisProt	DP00046.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9QXN1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	161452.
KEGG	rno:161452.
Organism-specific databases
CTD	51176.
RGD	620241. Lef1.
Phylogenomic databases
GeneTree	ENSGT00390000009964.
HOVERGEN	HBG000419.
InParanoid	Q9QXN1.
Gene expression databases
ArrayExpress	Q9QXN1.
Genevestigator	Q9QXN1.
GermOnline	ENSRNOG00000010121. Rattus norvegicus.
Family and domain databases
InterPro	IPR013558. CTNNB1-bd_N. IPR000910. HMG_HMG1/HMG2. IPR009071. HMG_superfamily. [Graphical view]
Gene3D	G3DSA:1.10.30.10. HMG-box. 1 hit.
KO	K04492.
Pfam	PF08347. CTNNB1_binding. 1 hit. PF00505. HMG_box. 1 hit. [Graphical view]
SMART	SM00398. HMG. 1 hit. [Graphical view]
SUPFAM	SSF47095. HMG-box. 1 hit.
PROSITE	PS50118. HMG_BOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	620971.

Entry information

Entry name

LEF1_RAT

Accession

Primary (citable) accession number: Q9QXN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program