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Reviewed, UniProtKB/Swiss-Prot Q9QXN1 (LEF1_RAT)

Last modified October 13, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Lymphoid enhancer-binding factor 1
      Short name=LEF-1
Gene names
Name: Lef1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length397 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 By similarity.

Subunit structure

Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with TLE1, PIASG, THOC4, EP300, MDFI and MDFIC By similarity.

Subcellular location

Nucleus By similarity. Note: Found in nuclear bodies upon PIASG binding By similarity.

Domain

Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.

Sequence similarities

Belongs to the TCF/LEF family.

Contains 1 HMG box DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 397397Lymphoid enhancer-binding factor 1
PRO_0000048597

Regions

DNA binding297 – 36569HMG box
Region1 – 6060CTNNB1-binding By similarity
Compositional bias6 – 116Poly-Gly
Compositional bias12 – 5039Asp/Glu-rich (acidic)
Compositional bias75 – 271197Pro-rich
Compositional bias372 – 3776Poly-Lys

Amino acid modifications

Modified residue931N6-acetyllysine By similarity
Cross-link25Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link267Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9QXN1-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: D6BEFF805CB526EA

FASTA39744,023
        10         20         30         40         50         60 
MPQLSGGGGG GDPELCATDE MIPFKDEGDP QKEKIFAEIS HPEEEGDLAD IKSSLVNESE 

        70         80         90        100        110        120 
IIPASNGHEV VGQTQSSQEP YHDKAREHPD DGKHPDGGLY NKGPSYSSYS GYIMMPNMNS 

       130        140        150        160        170        180 
DPYMSNGSLS PPIPRTSNKV PVVQPSHAVH PLTPLITYSD EHFSPGSHPS HIPSEVNPKQ 

       190        200        210        220        230        240 
GMSRHPPAPE MPTFYPLSPG GVGQITPPLG WQGQPVYPIT GGFRQAYPSS LSGDTSMSRF 

       250        260        270        280        290        300 
SHHMIPGPPG PHTTGIPHPA IVTPQVKQEH PHTDSDLMHV KPEHEQRKEQ EPKRPHIKKP 

       310        320        330        340        350        360 
LNAFMLYMKE MRANVVAECT LKESAAINQI LGRRWHALSR EEQAKYYELA RKERQLHMQL 

       370        380        390 
YPGWSARDNY GKKKKRKREK LQESTSGTGP RMTAAYI 

« Hide

References

[1]"Cloning of the lymphoid enhancer binding factor-1 (Lef-1) cDNA from rat kidney: homology to the mouse sequence."
Kobielak K., Kobielak A., Trzeciak W.H.
Acta Biochim. Pol. 46:885-888(1999) [PubMed: 10824856] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

AF198533 mRNA. Translation: AAF15601.1.
IPIIPI00213373.
RefSeqNP_569113.1.
UniGeneRn.21926

3D structure databases

HSSPHSSP built from PDB template 2LEF based on UniProtKB P27782.
SMRQ9QXN1. Positions 296-371.
DisProtDP00046.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9QXN1.

Genome annotation databases

EnsemblENSRNOT00000013694; ENSRNOP00000013694; ENSRNOG00000010121; Rattus norvegicus. [Genome view]
GeneID161452.
KEGGrno:161452.

Organism-specific databases

CTD161452.
RGD620241. Lef1.

Phylogenomic databases

HOVERGENQ9QXN1.

Gene expression databases

ArrayExpressQ9QXN1.
GenevestigatorQ9QXN1.
GermOnlineENSRNOG00000010121. Rattus norvegicus.

Family and domain databases

InterProIPR013558. CTNNB1_bd_N.
IPR000910. HMG_HMG1/HMG2.
[Graphical view]
Gene3DG3DSA:1.10.30.10. HMG-box. 1 hit.
PfamPF08347. CTNNB1_binding. 1 hit.
PF00505. HMG_box. 1 hit.
[Graphical view]
SMARTSM00398. HMG. 1 hit.
[Graphical view]
PROSITEPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio620971.

Entry information

Entry nameLEF1_RAT
AccessionPrimary (citable) accession number: Q9QXN1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: October 13, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents