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Protein

Protein Shroom3

Gene

Shroom3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls cell shape changes in the neuroepithelium during neural tube closure. Induces apical constriction in epithelial cells by promoting the apical accumulation of F-actin and myosin II, and probably by bundling stress fibers. Induces apicobasal cell elongation by redistributing gamma-tubulin and directing the assembly of robust apicobasal microtubule arrays.4 Publications

GO - Molecular functioni

  • actin binding Source: MGI

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • apical protein localization Source: HGNC
  • cell morphogenesis Source: HGNC
  • cellular pigment accumulation Source: HGNC
  • columnar/cuboidal epithelial cell development Source: MGI
  • neural tube closure Source: MGI
  • pattern specification process Source: MGI
  • regulation of cell shape Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell shape

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Shroom3
Gene namesi
Name:Shroom3
Synonyms:Kiaa1481, Shrm
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1351655. Shroom3.

Subcellular locationi

  • Cell junctionadherens junction 1 Publication
  • Cytoplasmcytoskeleton 1 Publication

  • Note: Colocalizes with F-actin in stress fibers and adherens junctions.

GO - Cellular componenti

  • adherens junction Source: MGI
  • apical junction complex Source: MGI
  • apical part of cell Source: MGI
  • apical plasma membrane Source: MGI
  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: MGI
  • microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Microtubule

Pathology & Biotechi

Disruption phenotypei

Death at birth due to defects in neural tube closure causing exencephaly, acrania, facial clefting and spina bifida.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19861986Protein Shroom3PRO_0000286067Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei212 – 2121PhosphoserineCombined sources
Modified residuei439 – 4391PhosphoserineCombined sources
Modified residuei443 – 4431PhosphoserineCombined sources
Modified residuei888 – 8881PhosphoserineBy similarity
Modified residuei909 – 9091PhosphothreonineCombined sources
Modified residuei912 – 9121PhosphoserineCombined sources
Modified residuei969 – 9691PhosphoserineBy similarity
Modified residuei1063 – 10631PhosphoserineCombined sources
Modified residuei1066 – 10661PhosphoserineCombined sources
Modified residuei1219 – 12191PhosphoserineCombined sources
Isoform 4 (identifier: Q9QXN0-4)
Modified residuei1175 – 11751PhosphoserineCombined sources
Modified residuei1179 – 11791PhosphoserineCombined sources
Isoform 3 (identifier: Q9QXN0-3)
Modified residuei1350 – 13501PhosphoserineCombined sources
Modified residuei1354 – 13541PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9QXN0.
PaxDbiQ9QXN0.
PeptideAtlasiQ9QXN0.
PRIDEiQ9QXN0.

PTM databases

iPTMnetiQ9QXN0.
PhosphoSiteiQ9QXN0.

Expressioni

Developmental stagei

At E8.75, strongly expressed in the cranial neuroepithelium, and also expressed in neural tube, paraxial mesoderm and gut. At E10.5, expressed in neural tube, forebrain, somites, ventral body wall, heart and gut. At E14.5, expression is restricted to skeletal muscle, tips of the digits and forebrain.1 Publication

Gene expression databases

BgeeiENSMUSG00000029381.
CleanExiMM_SHROOM3.

Interactioni

Subunit structurei

Interacts with F-actin.1 Publication

GO - Molecular functioni

  • actin binding Source: MGI

Protein-protein interaction databases

BioGridi205229. 2 interactions.
STRINGi10090.ENSMUSP00000108678.

Structurei

3D structure databases

ProteinModelPortaliQ9QXN0.
SMRiQ9QXN0. Positions 24-107, 1769-1945.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 10986PDZPROSITE-ProRule annotationAdd
BLAST
Domaini927 – 102397ASD1PROSITE-ProRule annotationAdd
BLAST
Domaini1659 – 1947289ASD2PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1844 – 189047Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi191 – 26878Ser-richAdd
BLAST
Compositional biasi1351 – 1540190Pro-richAdd
BLAST

Domaini

The ASD1 domain mediates F-actin binding.
The ASD2 domain is required for apical constriction induction.

Sequence similaritiesi

Belongs to the shroom family.Curated
Contains 1 ASD1 domain.PROSITE-ProRule annotation
Contains 1 ASD2 domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410II86. Eukaryota.
ENOG41113GY. LUCA.
HOGENOMiHOG000169233.
HOVERGENiHBG108489.
InParanoidiQ9QXN0.
KOiK18625.
PhylomeDBiQ9QXN0.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR014800. ASD1_dom.
IPR014799. ASD2_dom.
IPR001478. PDZ.
IPR030494. Shrm3.
IPR027685. Shroom_fam.
[Graphical view]
PANTHERiPTHR15012. PTHR15012. 7 hits.
PTHR15012:SF33. PTHR15012:SF33. 7 hits.
PfamiPF08688. ASD1. 1 hit.
PF08687. ASD2. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51306. ASD1. 1 hit.
PS51307. ASD2. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QXN0-1) [UniParc]FASTAAdd to basket
Also known as: L,a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKTPENLEEP SATPNPSRTP TERFVYLEAL LEGGAPWGFT LKGGLERGEP
60 70 80 90 100
LIISKIEEGG KADSVSSGLQ AGDEVIHINE VALSSPRREA VSLVKGSYKT
110 120 130 140 150
LRLVVRRDVC AAPGHADPGT SKSLSSELLT CSPQHRKATW SGGVKLRLKQ
160 170 180 190 200
RCSEPATRPH SWHTTKFGET QPDVSMMQIS QGTMGPPWHQ SYHSSSSTSD
210 220 230 240 250
LSNYDHAYLR RSPDQCSSQG SMESLEPSGG YPPCHLLSPA KSTSSIDQLG
260 270 280 290 300
HLHNKRDSAY SSFSTSSSIF EYPPPGGSAR ERSGSMDVIS ARGGLLEGMR
310 320 330 340 350
QADIRYVKTV YDTRRGVSSE YEVNPSALLL QGRDAHASAD SQGCAKWHSI
360 370 380 390 400
PRGKGTPSPS WSQQCSGSLE TATDNLPQKA GAPLPPTRSD SYAAFRHRER
410 420 430 440 450
PSSWSSLDQK RFCRPQTNSS GSQKTPFAED QLHTVPERSP ENSPPVKSKH
460 470 480 490 500
NYTQKAQPGQ PLLPTGIYPV PSPEPHFAQV PQPSVSSNGT VYPALVKESG
510 520 530 540 550
YTAAQGTCNK MATLDENGNQ NEASRPGFAF CQPLEHNSVT PVEKRPEPTA
560 570 580 590 600
KYIYKVHFSS VPENEDSSLK RHITPPHGHS PYPSERKNIH GGSRACSNHH
610 620 630 640 650
SLSSPQAQAL HVGDDRRPSR LSQPWEGDFQ EDHNANLRQK VEREGQGQGL
660 670 680 690 700
SGNSGRTRSA FSSLQNIPES LRRQSNVELG EAQEVHPGGR SKVEDPGRKA
710 720 730 740 750
GASDIRGYLD RSVSYPRPEG KMNAVDSVHS ADSRYEESPA PALPQTSGAS
760 770 780 790 800
QRRLSSSSSA APQYRKPHCS VLEKVSRIEE REQGRHRPLS VGSSAYGPGY
810 820 830 840 850
RPGRTGPTPS TSSSDLDDPK AGSVHFSEST EHLRNGEQNP PNGEAKQEEA
860 870 880 890 900
SRPQCSHLIR RAPADGRGPP ARGGEPSRPE ARLLRSQSTF QLYSEAEREA
910 920 930 940 950
SWSEDRPGTP ESPLLDAPFS RAYRNSIKDA QSRVLGATSF RRRDLEPGTP
960 970 980 990 1000
ATSRPWRPRP ASAHVGMRSP EAAVPSSSPH TPRERHSVTP AAPQAARRGP
1010 1020 1030 1040 1050
RRRLTVEQKK RSYSEPEKMN EVGVSEEAEP TPCGPPRPAQ PRFSESTVAD
1060 1070 1080 1090 1100
RRRIFERDGK ACSTLSLSGP ELKQFQQSAL ADYIQRKTGK RPTGAACTPE
1110 1120 1130 1140 1150
AGLRERAQSA YLQAGPAAPD GPGLASACSL SSLREPEALP RKEHTHPSAA
1160 1170 1180 1190 1200
DGPQAPRDRS SSFASGRLVG ERRRWDPQVP RQLLSGANCE PRGVQRMDGA
1210 1220 1230 1240 1250
PGGPPSWGMV AGKAGKSKSA EDLLERSDTL AVPVHVRSRS SPTSDKKGQD
1260 1270 1280 1290 1300
VLLREGSNFG FVKDPCCLAG PGPRSLSCSD KGQNELALPL HHPTPCWNGS
1310 1320 1330 1340 1350
GCKATVASSA PPESSGAADH LKQRRAPGPR PLSAGMHGHF PDARAASLSS
1360 1370 1380 1390 1400
PLPSPVPSAS PVPSSYRSQL AMDQQTGQQP PSSPASAVTQ PTSPRSPELS
1410 1420 1430 1440 1450
SPAYGLGEGM WKRTSLPQRP PPPWVKWAHA VREDGLAEDT LAPEFANLKH
1460 1470 1480 1490 1500
YRNQPSRPSS CSTSDPDTPG RISLRISESA LQPSPPPRGD YDDEVFMKDL
1510 1520 1530 1540 1550
HPKVTSSPTF EALPPPPPPS PPSEEPLVNG TDDFPPPPPP QALCEVLLDG
1560 1570 1580 1590 1600
EASTEAGSGP CRIPRVMVTR EGHVPGAAHS EGSQIMTATP PQTSAKGSEA
1610 1620 1630 1640 1650
ESNTPSSASA QPQLNGSPGK QLCPSQTRNL TYEPVERTQD LGKKTHAEPQ
1660 1670 1680 1690 1700
KTSEDIRTEA LAKEIVHQDK SLADILDPDS RMKTTMDLME GLFPGDASVL
1710 1720 1730 1740 1750
MDSGAKRKAL DITARRAGCE AKASDHKEAV SVLVNCPAYY SVSAAKAELL
1760 1770 1780 1790 1800
NKIKDMPEEL QEEEGQEDVN EKKAELIGSL THKLESLQEA KGSLLTDIKL
1810 1820 1830 1840 1850
NNALGEEVEA LISELCKPNE FDKYKMFIGD LDKVVNLLLS LSGRLARVEN
1860 1870 1880 1890 1900
VLRGLGEDAS KEERSSLNEK RKVLAGQHED ARELKENLDR RERVVLDILA
1910 1920 1930 1940 1950
NYLSAEQLQD YQHFVKMKST LLIEQRKLDD KIKLGQEQVR CLLESLPSDF
1960 1970 1980
RPKAGAISLP PALTGHATPG GTSVFGGVFP TLTSPL
Length:1,986
Mass (Da):214,888
Last modified:May 1, 2007 - v2
Checksum:i52C7A4AF6390D547
GO
Isoform 2 (identifier: Q9QXN0-2) [UniParc]FASTAAdd to basket
Also known as: S,b

The sequence of this isoform differs from the canonical sequence as follows:
     1-175: Missing.

Show »
Length:1,811
Mass (Da):195,981
Checksum:iDE2A33086EE355F8
GO
Isoform 3 (identifier: Q9QXN0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1354-1359: Missing.

Show »
Length:1,980
Mass (Da):214,349
Checksum:i18B675C987AAC60B
GO
Isoform 4 (identifier: Q9QXN0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-175: Missing.
     1354-1359: Missing.

Show »
Length:1,805
Mass (Da):195,442
Checksum:iB8099FC19E506415
GO

Sequence cautioni

The sequence AAF13269 differs from that shown. Reason: Frameshift at positions 1097 and 1124. Curated
The sequence AAF13270 differs from that shown. Reason: Frameshift at positions 1097 and 1124. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti409 – 4091Q → H in AAF13270 (PubMed:10589677).Curated
Sequence conflicti409 – 4091Q → H in AAF13269 (PubMed:10589677).Curated
Sequence conflicti617 – 6171R → K in AAF13270 (PubMed:10589677).Curated
Sequence conflicti617 – 6171R → K in AAF13269 (PubMed:10589677).Curated
Sequence conflicti683 – 6831Q → P in BAC98181 (PubMed:14621295).Curated
Sequence conflicti800 – 8001Y → N in AAF13270 (PubMed:10589677).Curated
Sequence conflicti800 – 8001Y → N in AAF13269 (PubMed:10589677).Curated
Sequence conflicti1078 – 10781S → N in AAF13270 (PubMed:10589677).Curated
Sequence conflicti1078 – 10781S → N in AAF13269 (PubMed:10589677).Curated
Sequence conflicti1239 – 12391R → K in AAF13270 (PubMed:10589677).Curated
Sequence conflicti1239 – 12391R → K in AAF13269 (PubMed:10589677).Curated
Sequence conflicti1293 – 12931P → H in AAF13270 (PubMed:10589677).Curated
Sequence conflicti1293 – 12931P → H in AAF13269 (PubMed:10589677).Curated
Sequence conflicti1318 – 13181A → S in BAC98181 (PubMed:14621295).Curated
Sequence conflicti1397 – 13971P → L in AAF13270 (PubMed:10589677).Curated
Sequence conflicti1397 – 13971P → L in AAF13269 (PubMed:10589677).Curated
Sequence conflicti1407 – 14082GE → EK in BAC98181 (PubMed:14621295).Curated
Sequence conflicti1419 – 14191R → Q in BAC98181 (PubMed:14621295).Curated
Sequence conflicti1423 – 14231P → L in BAC98181 (PubMed:14621295).Curated
Sequence conflicti1457 – 14571R → L in BAC98181 (PubMed:14621295).Curated
Sequence conflicti1770 – 17701N → Y in AAF13270 (PubMed:10589677).Curated
Sequence conflicti1770 – 17701N → Y in AAF13269 (PubMed:10589677).Curated
Sequence conflicti1967 – 19671A → G in BAE27948 (PubMed:16141072).Curated
Sequence conflicti1967 – 19671A → G in BAE37966 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 175175Missing in isoform 2 and isoform 4. 2 PublicationsVSP_024969Add
BLAST
Alternative sequencei1354 – 13596Missing in isoform 3 and isoform 4. 2 PublicationsVSP_024970

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199421 mRNA. Translation: AAF13269.1. Frameshift.
AF199422 mRNA. Translation: AAF13270.1. Frameshift.
AK147493 mRNA. Translation: BAE27948.1.
AK164918 mRNA. Translation: BAE37966.1.
AK129371 mRNA. Translation: BAC98181.1.
BC003909 mRNA. Translation: AAH03909.1.
CCDSiCCDS39154.1. [Q9QXN0-3]
CCDS39155.1. [Q9QXN0-4]
RefSeqiNP_001071063.1. NM_001077595.2.
NP_001071064.1. NM_001077596.2.
NP_056571.2. NM_015756.2.
XP_006535031.1. XM_006534968.1.
UniGeneiMm.46014.

Genome annotation databases

GeneIDi27428.
KEGGimmu:27428.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF199421 mRNA. Translation: AAF13269.1. Frameshift.
AF199422 mRNA. Translation: AAF13270.1. Frameshift.
AK147493 mRNA. Translation: BAE27948.1.
AK164918 mRNA. Translation: BAE37966.1.
AK129371 mRNA. Translation: BAC98181.1.
BC003909 mRNA. Translation: AAH03909.1.
CCDSiCCDS39154.1. [Q9QXN0-3]
CCDS39155.1. [Q9QXN0-4]
RefSeqiNP_001071063.1. NM_001077595.2.
NP_001071064.1. NM_001077596.2.
NP_056571.2. NM_015756.2.
XP_006535031.1. XM_006534968.1.
UniGeneiMm.46014.

3D structure databases

ProteinModelPortaliQ9QXN0.
SMRiQ9QXN0. Positions 24-107, 1769-1945.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205229. 2 interactions.
STRINGi10090.ENSMUSP00000108678.

PTM databases

iPTMnetiQ9QXN0.
PhosphoSiteiQ9QXN0.

Proteomic databases

MaxQBiQ9QXN0.
PaxDbiQ9QXN0.
PeptideAtlasiQ9QXN0.
PRIDEiQ9QXN0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi27428.
KEGGimmu:27428.

Organism-specific databases

CTDi57619.
MGIiMGI:1351655. Shroom3.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410II86. Eukaryota.
ENOG41113GY. LUCA.
HOGENOMiHOG000169233.
HOVERGENiHBG108489.
InParanoidiQ9QXN0.
KOiK18625.
PhylomeDBiQ9QXN0.

Miscellaneous databases

ChiTaRSiShroom3. mouse.
PROiQ9QXN0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029381.
CleanExiMM_SHROOM3.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR014800. ASD1_dom.
IPR014799. ASD2_dom.
IPR001478. PDZ.
IPR030494. Shrm3.
IPR027685. Shroom_fam.
[Graphical view]
PANTHERiPTHR15012. PTHR15012. 7 hits.
PTHR15012:SF33. PTHR15012:SF33. 7 hits.
PfamiPF08688. ASD1. 1 hit.
PF08687. ASD2. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS51306. ASD1. 1 hit.
PS51307. ASD2. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSHRM3_MOUSE
AccessioniPrimary (citable) accession number: Q9QXN0
Secondary accession number(s): Q3TNX1
, Q6ZPP9, Q99L16, Q9QXM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: May 1, 2007
Last modified: September 7, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.