Q9QXL7 (NDK7_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase 7
Short name=NDK 7
Short name=NDP kinase 7
EC=2.7.4.6

Alternative name(s):
nm23-R7

Gene names

Name:

Nme7

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.
Cofactor	Magnesium By similarity.
Tissue specificity	Expressed in the flagellum of epididymal sperm but not in testicular sperm (at protein level). Ref.2
Sequence similarities	Belongs to the NDK family. Contains 1 DM10 domain.
Mass spectrometry	Molecular mass is 44511.35 Da from positions 1 - 395. Determined by MALDI. Ref.2

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Nucleoside diphosphate kinase 7		PRO_0000137132
Regions
	Domain	22 – 110	89	DM10
Sites
	Active site	225	1	Pros-phosphohistidine intermediate By similarity
	Binding site	118	1	ATP By similarity
	Binding site	164	1	ATP By similarity
	Binding site	212	1	ATP By similarity
	Binding site	222	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QXL7 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 09EC4A204D92B38A	FASTA	395	44,540
	10 20 30 40 50 60 MKAGQQGRSC GLISPYLAPK NQSERFAFIA EWYDPNASLL RRYELLYYPV DGSVEMHDVK 70 80 90 100 110 120 NRRTFLKRTK YEDLRVEDLF IGNKVNVFSR QLVLIDYGDQ YTARQLGSRK EKTLALIKPD 130 140 150 160 170 180 AVSKAGEIIE MINKSGFTIT KLRMMTLSRK EAADFHVDHH SRPFYNELIQ FITSGPVIAM 190 200 210 220 230 240 EILRDDAICE WKRLLGPANS GIARSEAPGS VRALFGTDGI RNAAHGSDTF ESAAREMELF 250 260 270 280 290 300 FPSSGGCGPT NTAKFTNCTC CIIKPHAISE GMLGKILIAI RDACFEISAI QMFNMDRANV 310 320 330 340 350 360 EEFYEVYKGV LSDYNDMVTE LYSGPCVAIE IQQSNPTKTF REFCGPSDPE IARHLRPETL 370 380 390 RANFGKTKVQ NAVHCTDLPE DGLLEVQYFF KILDN

References

[1]	Mehus J.G., Johnson J.D., Lambeth D.O. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Differential proteomics leads to identification of domain specific epididymal sperm proteins." Suryawanshi A.R., Khan S.A., Gajbhiye R.K., Gurav M.Y., Khole V.V. J. Androl. 32:240-259(2011) [PubMed: 20966424] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY, MASS SPECTROMETRY. Strain: Holtzman. Tissue: Sperm.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF202049 mRNA. Translation: AAF20907.1.
IPI	IPI00213315.
RefSeq	NP_612541.1. NM_138532.1.
UniGene	Rn.82718.
3D structure databases
ProteinModelPortal	Q9QXL7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9QXL7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	171566.
KEGG	rno:171566.
UCSC	NM_138532. rat.
Organism-specific databases
CTD	29922.
RGD	619880. Nme7.
Phylogenomic databases
GeneTree	ENSGT00550000074206.
HOVERGEN	HBG006457.
OrthoDB	EOG4JHCFT.
Gene expression databases
ArrayExpress	Q9QXL7.
Genevestigator	Q9QXL7.
GermOnline	ENSRNOG00000002898. Rattus norvegicus.
Family and domain databases
InterPro	IPR011410. NDK7. IPR001564. Nucleoside_diP_kinase. IPR006602. Uncharacterised_DM10. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 2 hits.
KO	K00940.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 2 hits. [Graphical view]
PIRSF	PIRSF036503. NDK7. 1 hit.
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00676. DM10. 1 hit. SM00562. NDK. 2 hits. [Graphical view]
SUPFAM	SSF54919. NDK. 2 hits.
PROSITE	PS51336. DM10. 1 hit. PS00469. NDP_KINASES. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	622579.

Entry information

Entry name

NDK7_RAT

Accession

Primary (citable) accession number: Q9QXL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program