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Protein

E3 ubiquitin-protein ligase RAD18

Gene

Rad18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri25 – 64RING-typePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri201 – 224UBZ-typeAdd BLAST24

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: MGI
  • negative regulation of cell death Source: UniProtKB
  • negative regulation of DNA recombination Source: MGI
  • positive regulation of chromosome segregation Source: UniProtKB
  • postreplication repair Source: GO_Central
  • protein autoubiquitination Source: MGI
  • protein monoubiquitination Source: GO_Central
  • response to UV Source: MGI
  • spermatogenesis Source: MGI

Keywordsi

Molecular functionDNA-binding, Transferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-MMU-8866654. E3 ubiquitin ligases ubiquitinate target proteins.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RAD18 (EC:2.3.2.27)
Alternative name(s):
Postreplication repair protein RAD18
Short name:
mRAD18Sc
RING-type E3 ubiquitin transferase RAD18Curated
Gene namesi
Name:Rad18
Synonyms:Rad18sc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1890476. Rad18.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561501 – 509E3 ubiquitin-protein ligase RAD18Add BLAST509

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei99PhosphoserineCombined sources1
Modified residuei103PhosphoserineCombined sources1
Modified residuei158PhosphoserineBy similarity1
Modified residuei164PhosphoserineBy similarity1
Modified residuei485PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9QXK2.
MaxQBiQ9QXK2.
PRIDEiQ9QXK2.

PTM databases

iPTMnetiQ9QXK2.
PhosphoSitePlusiQ9QXK2.

Expressioni

Tissue specificityi

Expressed in thymus, spleen, brain, and ovary.

Gene expression databases

BgeeiENSMUSG00000030254.
CleanExiMM_RAD18.
ExpressionAtlasiQ9QXK2. baseline and differential.
GenevisibleiQ9QXK2. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with UBE2A and UBE2B, one homodimer binding one molecule of UBE2B. Interacts with HLTF. Interacts with SHPRH. Interacts with SPRTN; leading to enhance chromatin association of RAD18 and RAD18-mediated PCNA ubiquitination and translesion DNA synthesis. Interacts (via C-terminus and phosphorylated form) with SLF1 (via BRCT domains); this interaction is required for efficient repair of UV-induced DNA damage. Interacts with SLF2. Interacts with SMC5; this interaction is increased in a SLF1 or SLF2-dependent manner.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi208379. 3 interactors.
IntActiQ9QXK2. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ9QXK2.
SMRiQ9QXK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini248 – 282SAPPROSITE-ProRule annotationAdd BLAST35

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi232 – 240LR motif9

Sequence similaritiesi

Belongs to the RAD18 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri25 – 64RING-typePROSITE-ProRule annotationAdd BLAST40
Zinc fingeri201 – 224UBZ-typeAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000011230.
HOGENOMiHOG000234845.
HOVERGENiHBG054721.
InParanoidiQ9QXK2.
KOiK10627.
PhylomeDBiQ9QXK2.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR003034. SAP_dom.
IPR036361. SAP_dom_sf.
IPR006642. Znf_Rad18_put.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF02037. SAP. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
SM00513. SAP. 1 hit.
SM00734. ZnF_Rad18. 1 hit.
PROSITEiView protein in PROSITE
PS50800. SAP. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9QXK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVLAEPRWP PGLAVMKTID DLLRCGICFE YFNIAVIIPQ CSHNYCSLCI
60 70 80 90 100
RKFLSYKTQC PTCCVAVTEP DLRNNRLLDE LVKSMNFART HLLQFALESP
110 120 130 140 150
PISPVSSTSK KVVVKVHNAD AAQHPVKQAN RLMDKFLIRE TGDCVFELLG
160 170 180 190 200
KENERKFSPQ KELSTSAEIK ETSLLGKPVL GLSDANGPVT PSTSTMKLDT
210 220 230 240 250
KVSCPVCGVS IPENHINKHL DSCLSREEKK ESLRSSAHKR KPLPKTVYNL
260 270 280 290 300
LSDRDLKKKL KQYGLSVQGN KQQLIKRHQE FVHMYNAQCD ALHPKSAAEI
310 320 330 340 350
VQEIESMEKT RMRLEASKLN ENVMVFTKNQ TEKEIEEVHS EYRKKHQNAF
360 370 380 390 400
QLLVDQAKKG YKKTGRVSQA AAMRTDEPAE TLPSMRTDEP AETLPSMRTD
410 420 430 440 450
EPAETLPLMR ADEPAETLPS ECIAQEDNVS FSDTVSVTNH FPQPQLDSPG
460 470 480 490 500
PSEPERPDDS SSCTDILFSS DSDSCNRNDQ NREVSPQQTR RTRASECVEI

EPRNKRNKN
Length:509
Mass (Da):57,412
Last modified:June 6, 2002 - v2
Checksum:iBF7717D1A69738F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9W → C in AAF19193 (PubMed:11013078).Curated1
Sequence conflicti268Q → P in AAF19193 (PubMed:11013078).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF205278 mRNA. Translation: AAF19193.1.
AK012795 mRNA. Translation: BAB28475.1.
CCDSiCCDS39589.1.
RefSeqiNP_067360.2. NM_021385.2.
UniGeneiMm.103812.

Genome annotation databases

EnsembliENSMUST00000077088; ENSMUSP00000076341; ENSMUSG00000030254.
GeneIDi58186.
KEGGimmu:58186.
UCSCiuc009dec.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiRAD18_MOUSE
AccessioniPrimary (citable) accession number: Q9QXK2
Secondary accession number(s): Q9CZB8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: October 25, 2017
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families