E3 ubiquitin-protein ligase RAD18 - Mus musculus (Mouse)

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Q9QXK2 (RAD18_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase RAD18
EC=6.3.2.-

Alternative name(s):
Postreplication repair protein RAD18
Short name=mRAD18Sc

Gene names

Name:	Rad18
Synonyms:	Rad18sc

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with UBE2A and UBE2B. Interacts with HLTF and SHPRH. Interacts with SPRTN; leading to enhance chromatin association of RAD18 and RAD18-mediated PCNA ubiquitination and translesion DNA synthesis By similarity.
Subcellular location	Nucleus.
Tissue specificity	Expressed in thymus, spleen, brain, and ovary.
Sequence similarities	Belongs to the RAD18 family. Contains 1 RING-type zinc finger. Contains 1 SAP domain. Contains 1 UBZ-type zinc finger.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of DNA recombination Inferred from mutant phenotype PubMed 12509447. Source: MGI protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway response to DNA damage stimulus Inferred from mutant phenotype PubMed 12509447. Source: MGI response to UV Inferred from mutant phenotype PubMed 12509447. Source: MGI spermatogenesis Inferred from mutant phenotype PubMed 19068231. Source: MGI
Cellular_component	XY body Inferred from direct assay PubMed 15383616. Source: MGI chromatin Inferred from direct assay PubMed 15657431. Source: MGI nucleus Inferred from direct assay Ref.1 PubMed 19068231. Source: MGI
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW ligase activity Inferred from electronic annotation. Source: UniProtKB-KW polyubiquitin binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 509

509

E3 ubiquitin-protein ligase RAD18

PRO_0000056150

Regions

Domain

248 – 282

SAP

Zinc finger

25 – 64

RING-type

Zinc finger

201 – 224

UBZ-type

Motif

232 – 240

LR motif

Amino acid modifications

Modified residue

Phosphoserine By similarity

Modified residue

103

Phosphoserine By similarity

Modified residue

164

Phosphoserine By similarity

Modified residue

485

Phosphoserine By similarity

Experimental info

Sequence conflict

W → C in AAF19193. Ref.1

Sequence conflict

268

Q → P in AAF19193. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q9QXK2 [UniParc].

Last modified June 6, 2002. Version 2.
Checksum: BF7717D1A69738F9
Show »

FASTA

509

57,412

        10         20         30         40         50         60 
MEVLAEPRWP PGLAVMKTID DLLRCGICFE YFNIAVIIPQ CSHNYCSLCI RKFLSYKTQC 

        70         80         90        100        110        120 
PTCCVAVTEP DLRNNRLLDE LVKSMNFART HLLQFALESP PISPVSSTSK KVVVKVHNAD 

       130        140        150        160        170        180 
AAQHPVKQAN RLMDKFLIRE TGDCVFELLG KENERKFSPQ KELSTSAEIK ETSLLGKPVL 

       190        200        210        220        230        240 
GLSDANGPVT PSTSTMKLDT KVSCPVCGVS IPENHINKHL DSCLSREEKK ESLRSSAHKR 

       250        260        270        280        290        300 
KPLPKTVYNL LSDRDLKKKL KQYGLSVQGN KQQLIKRHQE FVHMYNAQCD ALHPKSAAEI 

       310        320        330        340        350        360 
VQEIESMEKT RMRLEASKLN ENVMVFTKNQ TEKEIEEVHS EYRKKHQNAF QLLVDQAKKG 

       370        380        390        400        410        420 
YKKTGRVSQA AAMRTDEPAE TLPSMRTDEP AETLPSMRTD EPAETLPLMR ADEPAETLPS 

       430        440        450        460        470        480 
ECIAQEDNVS FSDTVSVTNH FPQPQLDSPG PSEPERPDDS SSCTDILFSS DSDSCNRNDQ 

       490        500 
NREVSPQQTR RTRASECVEI EPRNKRNKN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of mRAD18Sc, a mouse homolog of the yeast postreplication repair gene RAD18." van der Laan R., Roest H.P., Hoogerbrugge J.W., Smit E.M.E., Slater R., Baarends W.M., Hoeijmakers J.H.J., Grootegoed J.A. Genomics 69:86-94(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF205278 mRNA. Translation: AAF19193.1. AK012795 mRNA. Translation: BAB28475.1.
IPI	IPI00470179.
RefSeq	NP_067360.2. NM_021385.2.
UniGene	Mm.103812.
3D structure databases
ProteinModelPortal	Q9QXK2.
SMR	Q9QXK2. Positions 8-95.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000070619.
PTM databases
PhosphoSite	Q9QXK2.
Proteomic databases
PRIDE	Q9QXK2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000077088; ENSMUSP00000076341; ENSMUSG00000030254.
GeneID	58186.
KEGG	mmu:58186.
UCSC	uc009dec.2. mouse.
Organism-specific databases
CTD	56852.
MGI	MGI:1890476. Rad18.
Phylogenomic databases
eggNOG	COG5432.
GeneTree	ENSGT00390000011230.
HOGENOM	HOG000234845.
HOVERGEN	HBG054721.
KO	K10627.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	Q9QXK2.
Bgee	Q9QXK2.
CleanEx	MM_RAD18.
Genevestigator	Q9QXK2.
GermOnline	ENSMUSG00000030254. Mus musculus.
Family and domain databases
Gene3D	1.10.720.30. 1 hit. 3.30.40.10. 1 hit.
InterPro	IPR003034. SAP_dom. IPR006642. Znf_Rad18_put. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. IPR017907. Znf_RING_CS. [Graphical view]
Pfam	PF02037. SAP. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00513. SAP. 1 hit. SM00734. ZnF_Rad18. 1 hit. [Graphical view]
PROSITE	PS50800. SAP. 1 hit. PS00518. ZF_RING_1. 1 hit. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	314151.
SOURCE	Search...

Entry information

Entry name

RAD18_MOUSE

Accession

Primary (citable) accession number: Q9QXK2
Secondary accession number(s): Q9CZB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	June 6, 2002
Last modified:	April 3, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents