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Protein

E3 ubiquitin-protein ligase RAD18

Gene

Rad18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri25 – 6440RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri201 – 22424UBZ-typeAdd
BLAST

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. polyubiquitin binding Source: UniProtKB
  3. ubiquitin protein ligase binding Source: MGI
  4. Y-form DNA binding Source: MGI
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular response to DNA damage stimulus Source: MGI
  2. DNA repair Source: UniProtKB-KW
  3. negative regulation of DNA recombination Source: MGI
  4. protein ubiquitination Source: UniProtKB-UniPathway
  5. response to UV Source: MGI
  6. spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RAD18 (EC:6.3.2.-)
Alternative name(s):
Postreplication repair protein RAD18
Short name:
mRAD18Sc
Gene namesi
Name:Rad18
Synonyms:Rad18sc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1890476. Rad18.

Subcellular locationi

GO - Cellular componenti

  1. chromatin Source: MGI
  2. nucleus Source: MGI
  3. replication fork Source: MGI
  4. XY body Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 509509E3 ubiquitin-protein ligase RAD18PRO_0000056150Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei99 – 991PhosphoserineBy similarity
Modified residuei103 – 1031PhosphoserineBy similarity
Modified residuei164 – 1641PhosphoserineBy similarity
Modified residuei485 – 4851PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9QXK2.
PRIDEiQ9QXK2.

PTM databases

PhosphoSiteiQ9QXK2.

Expressioni

Tissue specificityi

Expressed in thymus, spleen, brain, and ovary.

Gene expression databases

BgeeiQ9QXK2.
CleanExiMM_RAD18.
ExpressionAtlasiQ9QXK2. baseline and differential.
GenevestigatoriQ9QXK2.

Interactioni

Subunit structurei

Homodimer. Interacts with UBE2A and UBE2B, one homodimer binding one molecule of UBE2B. Interacts with HLTF and SHPRH. Interacts with SPRTN; leading to enhance chromatin association of RAD18 and RAD18-mediated PCNA ubiquitination and translesion DNA synthesis (By similarity).By similarity

Protein-protein interaction databases

BioGridi208379. 3 interactions.
STRINGi10090.ENSMUSP00000070619.

Structurei

3D structure databases

ProteinModelPortaliQ9QXK2.
SMRiQ9QXK2. Positions 8-95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini248 – 28235SAPPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi232 – 2409LR motif

Sequence similaritiesi

Belongs to the RAD18 family.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation
Contains 1 UBZ-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri25 – 6440RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri201 – 22424UBZ-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5432.
GeneTreeiENSGT00390000011230.
HOGENOMiHOG000234845.
HOVERGENiHBG054721.
InParanoidiQ9QXK2.
KOiK10627.
PhylomeDBiQ9QXK2.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR003034. SAP_dom.
IPR018957. Znf_C3HC4_RING-type.
IPR006642. Znf_Rad18_put.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF02037. SAP. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00513. SAP. 1 hit.
SM00734. ZnF_Rad18. 1 hit.
[Graphical view]
PROSITEiPS50800. SAP. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QXK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVLAEPRWP PGLAVMKTID DLLRCGICFE YFNIAVIIPQ CSHNYCSLCI
60 70 80 90 100
RKFLSYKTQC PTCCVAVTEP DLRNNRLLDE LVKSMNFART HLLQFALESP
110 120 130 140 150
PISPVSSTSK KVVVKVHNAD AAQHPVKQAN RLMDKFLIRE TGDCVFELLG
160 170 180 190 200
KENERKFSPQ KELSTSAEIK ETSLLGKPVL GLSDANGPVT PSTSTMKLDT
210 220 230 240 250
KVSCPVCGVS IPENHINKHL DSCLSREEKK ESLRSSAHKR KPLPKTVYNL
260 270 280 290 300
LSDRDLKKKL KQYGLSVQGN KQQLIKRHQE FVHMYNAQCD ALHPKSAAEI
310 320 330 340 350
VQEIESMEKT RMRLEASKLN ENVMVFTKNQ TEKEIEEVHS EYRKKHQNAF
360 370 380 390 400
QLLVDQAKKG YKKTGRVSQA AAMRTDEPAE TLPSMRTDEP AETLPSMRTD
410 420 430 440 450
EPAETLPLMR ADEPAETLPS ECIAQEDNVS FSDTVSVTNH FPQPQLDSPG
460 470 480 490 500
PSEPERPDDS SSCTDILFSS DSDSCNRNDQ NREVSPQQTR RTRASECVEI

EPRNKRNKN
Length:509
Mass (Da):57,412
Last modified:June 6, 2002 - v2
Checksum:iBF7717D1A69738F9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91W → C in AAF19193 (PubMed:11013078).Curated
Sequence conflicti268 – 2681Q → P in AAF19193 (PubMed:11013078).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF205278 mRNA. Translation: AAF19193.1.
AK012795 mRNA. Translation: BAB28475.1.
CCDSiCCDS39589.1.
RefSeqiNP_067360.2. NM_021385.2.
UniGeneiMm.103812.

Genome annotation databases

EnsembliENSMUST00000077088; ENSMUSP00000076341; ENSMUSG00000030254.
GeneIDi58186.
KEGGimmu:58186.
UCSCiuc009dec.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF205278 mRNA. Translation: AAF19193.1.
AK012795 mRNA. Translation: BAB28475.1.
CCDSiCCDS39589.1.
RefSeqiNP_067360.2. NM_021385.2.
UniGeneiMm.103812.

3D structure databases

ProteinModelPortaliQ9QXK2.
SMRiQ9QXK2. Positions 8-95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208379. 3 interactions.
STRINGi10090.ENSMUSP00000070619.

PTM databases

PhosphoSiteiQ9QXK2.

Proteomic databases

MaxQBiQ9QXK2.
PRIDEiQ9QXK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077088; ENSMUSP00000076341; ENSMUSG00000030254.
GeneIDi58186.
KEGGimmu:58186.
UCSCiuc009dec.2. mouse.

Organism-specific databases

CTDi56852.
MGIiMGI:1890476. Rad18.

Phylogenomic databases

eggNOGiCOG5432.
GeneTreeiENSGT00390000011230.
HOGENOMiHOG000234845.
HOVERGENiHBG054721.
InParanoidiQ9QXK2.
KOiK10627.
PhylomeDBiQ9QXK2.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiRad18. mouse.
NextBioi314151.
PROiQ9QXK2.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QXK2.
CleanExiMM_RAD18.
ExpressionAtlasiQ9QXK2. baseline and differential.
GenevestigatoriQ9QXK2.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR003034. SAP_dom.
IPR018957. Znf_C3HC4_RING-type.
IPR006642. Znf_Rad18_put.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF02037. SAP. 1 hit.
PF00097. zf-C3HC4. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00513. SAP. 1 hit.
SM00734. ZnF_Rad18. 1 hit.
[Graphical view]
PROSITEiPS50800. SAP. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of mRAD18Sc, a mouse homolog of the yeast postreplication repair gene RAD18."
    van der Laan R., Roest H.P., Hoogerbrugge J.W., Smit E.M.E., Slater R., Baarends W.M., Hoeijmakers J.H.J., Grootegoed J.A.
    Genomics 69:86-94(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.

Entry informationi

Entry nameiRAD18_MOUSE
AccessioniPrimary (citable) accession number: Q9QXK2
Secondary accession number(s): Q9CZB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: March 4, 2015
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.