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Protein

Glycine N-methyltransferase

Gene

Gnmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine (By similarity).By similarity1 Publication

Catalytic activityi

S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei31S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei34SubstratePROSITE-ProRule annotation1
Binding sitei41S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei65S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei86S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei137S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Binding sitei139SubstratePROSITE-ProRule annotation1
Binding sitei176SubstratePROSITE-ProRule annotation1
Binding sitei221SubstratePROSITE-ProRule annotation1

GO - Molecular functioni

  • folic acid binding Source: UniProtKB-KW
  • glycine binding Source: UniProtKB
  • glycine N-methyltransferase activity Source: UniProtKB
  • identical protein binding Source: MGI

GO - Biological processi

  • glycogen metabolic process Source: MGI
  • methionine metabolic process Source: MGI
  • one-carbon metabolic process Source: MGI
  • protein homotetramerization Source: UniProtKB
  • regulation of gluconeogenesis Source: MGI
  • S-adenosylmethionine metabolic process Source: UniProtKB

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandFolate-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.20 3474
ReactomeiR-MMU-389661 Glyoxylate metabolism and glycine degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine N-methyltransferase (EC:2.1.1.20)
Gene namesi
Name:Gnmt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1202304 Gnmt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000875252 – 293Glycine N-methyltransferaseAdd BLAST292

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylvalineBy similarity1
Modified residuei10PhosphoserineCombined sources1
Modified residuei34PhosphotyrosineCombined sources1
Modified residuei46N6-succinyllysineCombined sources1
Modified residuei191N6-succinyllysineCombined sources1
Modified residuei196N6-succinyllysineCombined sources1
Modified residuei201N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9QXF8
PaxDbiQ9QXF8
PeptideAtlasiQ9QXF8
PRIDEiQ9QXF8

PTM databases

iPTMnetiQ9QXF8
PhosphoSitePlusiQ9QXF8

Expressioni

Gene expression databases

BgeeiENSMUSG00000002769
CleanExiMM_GNMT
GenevisibleiQ9QXF8 MM

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi1999951 interactor.
IntActiQ9QXF8 3 interactors.
MINTiQ9QXF8
STRINGi10090.ENSMUSP00000002846

Structurei

Secondary structure

1293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 18Combined sources3
Turni21 – 24Combined sources4
Helixi26 – 35Combined sources10
Beta strandi39 – 41Combined sources3
Helixi43 – 55Combined sources13
Beta strandi60 – 65Combined sources6
Helixi70 – 77Combined sources8
Beta strandi81 – 87Combined sources7
Helixi89 – 101Combined sources13
Turni102 – 104Combined sources3
Helixi106 – 109Combined sources4
Beta strandi112 – 115Combined sources4
Helixi118 – 120Combined sources3
Helixi122 – 124Combined sources3
Beta strandi131 – 136Combined sources6
Helixi141 – 143Combined sources3
Beta strandi148 – 151Combined sources4
Helixi152 – 162Combined sources11
Beta strandi165 – 176Combined sources12
Helixi178 – 184Combined sources7
Beta strandi193 – 195Combined sources3
Beta strandi201 – 210Combined sources10
Beta strandi213 – 224Combined sources12
Beta strandi236 – 243Combined sources8
Helixi248 – 257Combined sources10
Turni258 – 261Combined sources4
Beta strandi263 – 273Combined sources11
Beta strandi283 – 291Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R8XX-ray2.95A/B2-293[»]
1R8YX-ray3.00A/B/C/D/E/F/G/H2-293[»]
ProteinModelPortaliQ9QXF8
SMRiQ9QXF8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QXF8

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni117 – 118S-adenosyl-L-methionine bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IF1C Eukaryota
ENOG410ZWXN LUCA
GeneTreeiENSGT00390000006845
HOGENOMiHOG000276537
HOVERGENiHBG051748
InParanoidiQ9QXF8
KOiK00552
OMAiQAYIPCY
OrthoDBiEOG091G0J3Z
PhylomeDBiQ9QXF8
TreeFamiTF324814

Family and domain databases

InterProiView protein in InterPro
IPR014369 Gly/Sar_N_MeTrfase
IPR025714 Methyltranfer_dom
IPR029063 SAM-dependent_MTases
PANTHERiPTHR16458 PTHR16458, 1 hit
PfamiView protein in Pfam
PF13847 Methyltransf_31, 1 hit
PIRSFiPIRSF000385 Gly_N-mtase, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51600 SAM_GNMT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QXF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL
60 70 80 90 100
GLLRQHGCHR VLDVACGTGV DSIMLVEEGF SVMSVDASDK MLKYALKERW
110 120 130 140 150
NRRKEPSFDN WVIEEANWLT LDKDVLSGDG FDAVICLGNS FAHLPDCKGD
160 170 180 190 200
QSEHRLALKN IASMVRPGGL LVIDHRNYDY ILSTGCAPPG KNIYYKSDLT
210 220 230 240 250
KDITTSVLTV NNKAHMVTLD YTVQVPGTGR DGSPGFSKFR LSYYPHCLAS
260 270 280 290
FTELVRAAFG GRCQHSVLGD FKPYKPGQAY VPCYFIHVLK KTD
Length:293
Mass (Da):32,675
Last modified:January 23, 2007 - v3
Checksum:i264F0ACB6BBF3CDA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti157A → E in AAH14283 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89664 mRNA Translation: BAA88218.1
AF325352 Genomic DNA Translation: AAK00338.1
AY054408 Genomic DNA Translation: AAL06142.1
BC014283 mRNA Translation: AAH14283.1
CCDSiCCDS28838.1
RefSeqiNP_034451.1, NM_010321.1
UniGeneiMm.29395

Genome annotation databases

EnsembliENSMUST00000002846; ENSMUSP00000002846; ENSMUSG00000002769
GeneIDi14711
KEGGimmu:14711
UCSCiuc008cue.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGNMT_MOUSE
AccessioniPrimary (citable) accession number: Q9QXF8
Secondary accession number(s): Q91WN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: January 23, 2007
Last modified: February 28, 2018
This is version 130 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome