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Q9QXE2

- DPOLL_MOUSE

UniProt

Q9QXE2 - DPOLL_MOUSE

Protein

DNA polymerase lambda

Gene

Poll

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity By similarity.By similarity

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    Cofactori

    Manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei310 – 3101Schiff-base intermediate with DNABy similarity
    Metal bindingi425 – 4251ManganeseBy similarity
    Metal bindingi427 – 4271ManganeseBy similarity
    Metal bindingi488 – 4881ManganeseBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA-directed DNA polymerase activity Source: MGI
    3. lyase activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. base-excision repair, gap-filling Source: MGI
    2. DNA-dependent DNA replication Source: GOC
    3. nucleotide-excision repair Source: UniProtKB

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Lyase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA damage, DNA repair, DNA replication, DNA synthesis

    Keywords - Ligandi

    DNA-binding, Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase lambda (EC:2.7.7.7, EC:4.2.99.-)
    Short name:
    Pol Lambda
    Alternative name(s):
    DNA polymerase kappa
    Gene namesi
    Name:Poll
    Synonyms:Polk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1889000. Poll.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 573573DNA polymerase lambdaPRO_0000218785Add
    BLAST

    Proteomic databases

    PRIDEiQ9QXE2.

    PTM databases

    PhosphoSiteiQ9QXE2.

    Expressioni

    Gene expression databases

    BgeeiQ9QXE2.
    CleanExiMM_POLK.
    MM_POLL.
    GenevestigatoriQ9QXE2.

    Interactioni

    Subunit structurei

    Binds PCNA.By similarity

    Protein-protein interaction databases

    MINTiMINT-1031713.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QXE2.
    SMRiQ9QXE2. Positions 35-134, 240-573.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 13197BRCTPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni263 – 27715DNA bindingBy similarityAdd
    BLAST
    Regioni343 – 3464DNA bindingBy similarity
    Regioni418 – 42710Involved in primer bindingBy similarity
    Regioni464 – 50340DNA bindingBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA polymerase type-X family.Curated
    Contains 1 BRCT domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1796.
    GeneTreeiENSGT00530000063002.
    HOGENOMiHOG000007787.
    HOVERGENiHBG002788.
    InParanoidiQ9QXE2.
    KOiK03512.
    OMAiWTEELIP.
    OrthoDBiEOG73Z2SX.
    PhylomeDBiQ9QXE2.
    TreeFamiTF103011.

    Family and domain databases

    Gene3Di1.10.150.110. 1 hit.
    3.30.210.10. 1 hit.
    3.40.50.10190. 1 hit.
    InterProiIPR001357. BRCT_dom.
    IPR002054. DNA-dir_DNA_pol_X.
    IPR019843. DNA_pol-X_BS.
    IPR010996. DNA_pol_b-like_N.
    IPR028207. DNA_pol_B_palm_palm.
    IPR018944. DNA_pol_lambd_fingers_domain.
    IPR022312. DNA_pol_X.
    IPR002008. DNA_pol_X_beta-like.
    IPR027421. DNA_pol_X_lyase_dom.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR029398. PolB_thumb.
    [Graphical view]
    PfamiPF14792. DNA_pol_B_palm. 1 hit.
    PF14791. DNA_pol_B_thumb. 1 hit.
    PF10391. DNA_pol_lambd_f. 1 hit.
    [Graphical view]
    PRINTSiPR00869. DNAPOLX.
    PR00870. DNAPOLXBETA.
    SMARTiSM00278. HhH1. 1 hit.
    SM00483. POLXc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47802. SSF47802. 1 hit.
    SSF52113. SSF52113. 1 hit.
    PROSITEiPS50172. BRCT. 1 hit.
    PS00522. DNA_POLYMERASE_X. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9QXE2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPQGIVKAF PKRKKSHADL SSKALAKIPK REVGEARGWL SSLRAHIMPA    50
    GIGRARAELF EKQIIHHGGQ VCSAQAPGVT HIVVDEDMDY ERALRLLRLP 100
    QLPPGAQLVK STWLSLCLQE GRLTDTEGFS LPMPKRSLDE PQPSKSGQDA 150
    SAPGTQRDLP RTTLSLSPPH TRAVSPPPTA EKPSRTQAQL SSEDETSDGE 200
    GPQVSSADLQ ALITGHYPTP PEEDGGPDPA PEALDKWVCA QPSSQKATNY 250
    NLHITEKLEV LAKAYSVQGD KWRALGYAKA INALKSFHKP VSSYQEACSI 300
    PGIGKRMAEK VMEILESGHL RKLDHISDSV PVLELFSNIW GAGTKTAQMW 350
    YHQGFRNLED LQSLGSLTAQ QAIGLKHYDD FLDRMPREEA AEIEQTVRIS 400
    AQAFNPGLLC VACGSYRRGK MTCGDVDVLI THPDGRSHRG IFSCLLDSLR 450
    QQGFLTDDLV SQEENGQQQK YLGVCRLPGP GKRHRRLDII VVPYCEFACA 500
    LLYFTGSAHF NRSMRALAKT KGMSLSEHAL SAAVVRNSQG VKVGPGQVLP 550
    TPTEKDVFKH LGLPYREPAE RDW 573
    Length:573
    Mass (Da):62,943
    Last modified:May 1, 2000 - v1
    Checksum:i5B2EA4D1597E06A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ131889 mRNA. Translation: CAB65241.1.
    AF176099 mRNA. Translation: AAF27553.1.
    BC004767 mRNA. Translation: AAH04767.1.
    AK003392 mRNA. Translation: BAB22759.1.
    CCDSiCCDS29861.1.
    RefSeqiNP_064416.1. NM_020032.2.
    XP_006527295.1. XM_006527232.1.
    UniGeneiMm.46509.

    Genome annotation databases

    EnsembliENSMUST00000026239; ENSMUSP00000026239; ENSMUSG00000025218.
    GeneIDi56626.
    KEGGimmu:56626.
    UCSCiuc008hrc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ131889 mRNA. Translation: CAB65241.1 .
    AF176099 mRNA. Translation: AAF27553.1 .
    BC004767 mRNA. Translation: AAH04767.1 .
    AK003392 mRNA. Translation: BAB22759.1 .
    CCDSi CCDS29861.1.
    RefSeqi NP_064416.1. NM_020032.2.
    XP_006527295.1. XM_006527232.1.
    UniGenei Mm.46509.

    3D structure databases

    ProteinModelPortali Q9QXE2.
    SMRi Q9QXE2. Positions 35-134, 240-573.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1031713.

    PTM databases

    PhosphoSitei Q9QXE2.

    Proteomic databases

    PRIDEi Q9QXE2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026239 ; ENSMUSP00000026239 ; ENSMUSG00000025218 .
    GeneIDi 56626.
    KEGGi mmu:56626.
    UCSCi uc008hrc.1. mouse.

    Organism-specific databases

    CTDi 27343.
    MGIi MGI:1889000. Poll.

    Phylogenomic databases

    eggNOGi COG1796.
    GeneTreei ENSGT00530000063002.
    HOGENOMi HOG000007787.
    HOVERGENi HBG002788.
    InParanoidi Q9QXE2.
    KOi K03512.
    OMAi WTEELIP.
    OrthoDBi EOG73Z2SX.
    PhylomeDBi Q9QXE2.
    TreeFami TF103011.

    Miscellaneous databases

    NextBioi 313055.
    PROi Q9QXE2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9QXE2.
    CleanExi MM_POLK.
    MM_POLL.
    Genevestigatori Q9QXE2.

    Family and domain databases

    Gene3Di 1.10.150.110. 1 hit.
    3.30.210.10. 1 hit.
    3.40.50.10190. 1 hit.
    InterProi IPR001357. BRCT_dom.
    IPR002054. DNA-dir_DNA_pol_X.
    IPR019843. DNA_pol-X_BS.
    IPR010996. DNA_pol_b-like_N.
    IPR028207. DNA_pol_B_palm_palm.
    IPR018944. DNA_pol_lambd_fingers_domain.
    IPR022312. DNA_pol_X.
    IPR002008. DNA_pol_X_beta-like.
    IPR027421. DNA_pol_X_lyase_dom.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR029398. PolB_thumb.
    [Graphical view ]
    Pfami PF14792. DNA_pol_B_palm. 1 hit.
    PF14791. DNA_pol_B_thumb. 1 hit.
    PF10391. DNA_pol_lambd_f. 1 hit.
    [Graphical view ]
    PRINTSi PR00869. DNAPOLX.
    PR00870. DNAPOLXBETA.
    SMARTi SM00278. HhH1. 1 hit.
    SM00483. POLXc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47802. SSF47802. 1 hit.
    SSF52113. SSF52113. 1 hit.
    PROSITEi PS50172. BRCT. 1 hit.
    PS00522. DNA_POLYMERASE_X. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA polymerase kappa, a new mammalian meiotic polymerase."
      Garcia M., Dominguez O., Saniger M.L., Garcia M.J., Martinez C., Bernad A., Blanco L.
      Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Testis.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Spleen.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 262-573.
      Strain: C57BL/6J.
      Tissue: Embryo.

    Entry informationi

    Entry nameiDPOLL_MOUSE
    AccessioniPrimary (citable) accession number: Q9QXE2
    Secondary accession number(s): Q9CTJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3