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Q9QXE2

- DPOLL_MOUSE

UniProt

Q9QXE2 - DPOLL_MOUSE

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Protein
DNA polymerase lambda
Gene
Poll, Polk
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity By similarity.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei310 – 3101Schiff-base intermediate with DNA By similarity
Metal bindingi425 – 4251Manganese By similarity
Metal bindingi427 – 4271Manganese By similarity
Metal bindingi488 – 4881Manganese By similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA-directed DNA polymerase activity Source: MGI
  3. lyase activity Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA-dependent DNA replication Source: GOC
  2. base-excision repair, gap-filling Source: MGI
  3. nucleotide-excision repair Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Lyase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, DNA synthesis

Keywords - Ligandi

DNA-binding, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase lambda (EC:2.7.7.7, EC:4.2.99.-)
Short name:
Pol Lambda
Alternative name(s):
DNA polymerase kappa
Gene namesi
Name:Poll
Synonyms:Polk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1889000. Poll.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 573573DNA polymerase lambda
PRO_0000218785Add
BLAST

Proteomic databases

PRIDEiQ9QXE2.

PTM databases

PhosphoSiteiQ9QXE2.

Expressioni

Gene expression databases

BgeeiQ9QXE2.
CleanExiMM_POLK.
MM_POLL.
GenevestigatoriQ9QXE2.

Interactioni

Subunit structurei

Binds PCNA By similarity.

Protein-protein interaction databases

MINTiMINT-1031713.

Structurei

3D structure databases

ProteinModelPortaliQ9QXE2.
SMRiQ9QXE2. Positions 35-134, 240-573.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 13197BRCT
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni263 – 27715DNA binding By similarity
Add
BLAST
Regioni343 – 3464DNA binding By similarity
Regioni418 – 42710Involved in primer binding By similarity
Regioni464 – 50340DNA binding By similarity
Add
BLAST

Sequence similaritiesi

Contains 1 BRCT domain.

Phylogenomic databases

eggNOGiCOG1796.
GeneTreeiENSGT00530000063002.
HOGENOMiHOG000007787.
HOVERGENiHBG002788.
InParanoidiQ9QXE2.
KOiK03512.
OMAiWTEELIP.
OrthoDBiEOG73Z2SX.
PhylomeDBiQ9QXE2.
TreeFamiTF103011.

Family and domain databases

Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR002008. DNA_pol_X_beta-like.
IPR027421. DNA_pol_X_lyase_dom.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR029398. PolB_thumb.
[Graphical view]
PfamiPF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
[Graphical view]
PRINTSiPR00869. DNAPOLX.
PR00870. DNAPOLXBETA.
SMARTiSM00278. HhH1. 1 hit.
SM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QXE2-1 [UniParc]FASTAAdd to Basket

« Hide

MDPQGIVKAF PKRKKSHADL SSKALAKIPK REVGEARGWL SSLRAHIMPA    50
GIGRARAELF EKQIIHHGGQ VCSAQAPGVT HIVVDEDMDY ERALRLLRLP 100
QLPPGAQLVK STWLSLCLQE GRLTDTEGFS LPMPKRSLDE PQPSKSGQDA 150
SAPGTQRDLP RTTLSLSPPH TRAVSPPPTA EKPSRTQAQL SSEDETSDGE 200
GPQVSSADLQ ALITGHYPTP PEEDGGPDPA PEALDKWVCA QPSSQKATNY 250
NLHITEKLEV LAKAYSVQGD KWRALGYAKA INALKSFHKP VSSYQEACSI 300
PGIGKRMAEK VMEILESGHL RKLDHISDSV PVLELFSNIW GAGTKTAQMW 350
YHQGFRNLED LQSLGSLTAQ QAIGLKHYDD FLDRMPREEA AEIEQTVRIS 400
AQAFNPGLLC VACGSYRRGK MTCGDVDVLI THPDGRSHRG IFSCLLDSLR 450
QQGFLTDDLV SQEENGQQQK YLGVCRLPGP GKRHRRLDII VVPYCEFACA 500
LLYFTGSAHF NRSMRALAKT KGMSLSEHAL SAAVVRNSQG VKVGPGQVLP 550
TPTEKDVFKH LGLPYREPAE RDW 573
Length:573
Mass (Da):62,943
Last modified:May 1, 2000 - v1
Checksum:i5B2EA4D1597E06A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ131889 mRNA. Translation: CAB65241.1.
AF176099 mRNA. Translation: AAF27553.1.
BC004767 mRNA. Translation: AAH04767.1.
AK003392 mRNA. Translation: BAB22759.1.
CCDSiCCDS29861.1.
RefSeqiNP_064416.1. NM_020032.2.
XP_006527295.1. XM_006527232.1.
UniGeneiMm.46509.

Genome annotation databases

EnsembliENSMUST00000026239; ENSMUSP00000026239; ENSMUSG00000025218.
GeneIDi56626.
KEGGimmu:56626.
UCSCiuc008hrc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ131889 mRNA. Translation: CAB65241.1 .
AF176099 mRNA. Translation: AAF27553.1 .
BC004767 mRNA. Translation: AAH04767.1 .
AK003392 mRNA. Translation: BAB22759.1 .
CCDSi CCDS29861.1.
RefSeqi NP_064416.1. NM_020032.2.
XP_006527295.1. XM_006527232.1.
UniGenei Mm.46509.

3D structure databases

ProteinModelPortali Q9QXE2.
SMRi Q9QXE2. Positions 35-134, 240-573.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1031713.

PTM databases

PhosphoSitei Q9QXE2.

Proteomic databases

PRIDEi Q9QXE2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026239 ; ENSMUSP00000026239 ; ENSMUSG00000025218 .
GeneIDi 56626.
KEGGi mmu:56626.
UCSCi uc008hrc.1. mouse.

Organism-specific databases

CTDi 27343.
MGIi MGI:1889000. Poll.

Phylogenomic databases

eggNOGi COG1796.
GeneTreei ENSGT00530000063002.
HOGENOMi HOG000007787.
HOVERGENi HBG002788.
InParanoidi Q9QXE2.
KOi K03512.
OMAi WTEELIP.
OrthoDBi EOG73Z2SX.
PhylomeDBi Q9QXE2.
TreeFami TF103011.

Miscellaneous databases

NextBioi 313055.
PROi Q9QXE2.
SOURCEi Search...

Gene expression databases

Bgeei Q9QXE2.
CleanExi MM_POLK.
MM_POLL.
Genevestigatori Q9QXE2.

Family and domain databases

Gene3Di 1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProi IPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR002008. DNA_pol_X_beta-like.
IPR027421. DNA_pol_X_lyase_dom.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR029398. PolB_thumb.
[Graphical view ]
Pfami PF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
[Graphical view ]
PRINTSi PR00869. DNAPOLX.
PR00870. DNAPOLXBETA.
SMARTi SM00278. HhH1. 1 hit.
SM00483. POLXc. 1 hit.
[Graphical view ]
SUPFAMi SSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA polymerase kappa, a new mammalian meiotic polymerase."
    Garcia M., Dominguez O., Saniger M.L., Garcia M.J., Martinez C., Bernad A., Blanco L.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 262-573.
    Strain: C57BL/6J.
    Tissue: Embryo.

Entry informationi

Entry nameiDPOLL_MOUSE
AccessioniPrimary (citable) accession number: Q9QXE2
Secondary accession number(s): Q9CTJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi