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Q9QXE2 (DPOLL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase lambda

Short name=Pol Lambda
EC=2.7.7.7
EC=4.2.99.-
Alternative name(s):
DNA polymerase kappa
Gene names
Name:Poll
Synonyms:Polk
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length573 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity By similarity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactor

Manganese By similarity.

Subunit structure

Binds PCNA By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the DNA polymerase type-X family.

Contains 1 BRCT domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 573573DNA polymerase lambda
PRO_0000218785

Regions

Domain35 – 13197BRCT
Region263 – 27715DNA binding By similarity
Region343 – 3464DNA binding By similarity
Region418 – 42710Involved in primer binding By similarity
Region464 – 50340DNA binding By similarity

Sites

Active site3101Schiff-base intermediate with DNA By similarity
Metal binding4251Manganese By similarity
Metal binding4271Manganese By similarity
Metal binding4881Manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9QXE2 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 5B2EA4D1597E06A2

FASTA57362,943
        10         20         30         40         50         60 
MDPQGIVKAF PKRKKSHADL SSKALAKIPK REVGEARGWL SSLRAHIMPA GIGRARAELF 

        70         80         90        100        110        120 
EKQIIHHGGQ VCSAQAPGVT HIVVDEDMDY ERALRLLRLP QLPPGAQLVK STWLSLCLQE 

       130        140        150        160        170        180 
GRLTDTEGFS LPMPKRSLDE PQPSKSGQDA SAPGTQRDLP RTTLSLSPPH TRAVSPPPTA 

       190        200        210        220        230        240 
EKPSRTQAQL SSEDETSDGE GPQVSSADLQ ALITGHYPTP PEEDGGPDPA PEALDKWVCA 

       250        260        270        280        290        300 
QPSSQKATNY NLHITEKLEV LAKAYSVQGD KWRALGYAKA INALKSFHKP VSSYQEACSI 

       310        320        330        340        350        360 
PGIGKRMAEK VMEILESGHL RKLDHISDSV PVLELFSNIW GAGTKTAQMW YHQGFRNLED 

       370        380        390        400        410        420 
LQSLGSLTAQ QAIGLKHYDD FLDRMPREEA AEIEQTVRIS AQAFNPGLLC VACGSYRRGK 

       430        440        450        460        470        480 
MTCGDVDVLI THPDGRSHRG IFSCLLDSLR QQGFLTDDLV SQEENGQQQK YLGVCRLPGP 

       490        500        510        520        530        540 
GKRHRRLDII VVPYCEFACA LLYFTGSAHF NRSMRALAKT KGMSLSEHAL SAAVVRNSQG 

       550        560        570 
VKVGPGQVLP TPTEKDVFKH LGLPYREPAE RDW 

« Hide

References

« Hide 'large scale' references
[1]"DNA polymerase kappa, a new mammalian meiotic polymerase."
Garcia M., Dominguez O., Saniger M.L., Garcia M.J., Martinez C., Bernad A., Blanco L.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[2]"Two novel human and mouse DNA polymerases of the polX family."
Aoufouchi S., Flatter E., Dahan A., Faili A., Bertocci B., Storck S., Delbos F., Cocea L., Gupta N., Weill J.-C., Reynaud C.-A.
Nucleic Acids Res. 28:3684-3693(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Spleen.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 262-573.
Strain: C57BL/6J.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ131889 mRNA. Translation: CAB65241.1.
AF176099 mRNA. Translation: AAF27553.1.
BC004767 mRNA. Translation: AAH04767.1.
AK003392 mRNA. Translation: BAB22759.1.
CCDSCCDS29861.1.
RefSeqNP_064416.1. NM_020032.2.
XP_006527295.1. XM_006527232.1.
UniGeneMm.46509.

3D structure databases

ProteinModelPortalQ9QXE2.
SMRQ9QXE2. Positions 35-134, 240-573.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1031713.

PTM databases

PhosphoSiteQ9QXE2.

Proteomic databases

PRIDEQ9QXE2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026239; ENSMUSP00000026239; ENSMUSG00000025218.
GeneID56626.
KEGGmmu:56626.
UCSCuc008hrc.1. mouse.

Organism-specific databases

CTD27343.
MGIMGI:1889000. Poll.

Phylogenomic databases

eggNOGCOG1796.
GeneTreeENSGT00530000063002.
HOGENOMHOG000007787.
HOVERGENHBG002788.
InParanoidQ9QXE2.
KOK03512.
OMAWTEELIP.
OrthoDBEOG73Z2SX.
PhylomeDBQ9QXE2.
TreeFamTF103011.

Gene expression databases

BgeeQ9QXE2.
CleanExMM_POLK.
MM_POLL.
GenevestigatorQ9QXE2.

Family and domain databases

Gene3D1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR002008. DNA_pol_X_beta-like.
IPR027421. DNA_pol_X_lyase_dom.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR029398. PolB_thumb.
[Graphical view]
PfamPF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
[Graphical view]
PRINTSPR00869. DNAPOLX.
PR00870. DNAPOLXBETA.
SMARTSM00278. HhH1. 1 hit.
SM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
SSF81585. SSF81585. 1 hit.
PROSITEPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio313055.
PROQ9QXE2.
SOURCESearch...

Entry information

Entry nameDPOLL_MOUSE
AccessionPrimary (citable) accession number: Q9QXE2
Secondary accession number(s): Q9CTJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot