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Protein

2-hydroxyacyl-CoA lyase 1

Gene

Hacl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.By similarity

Catalytic activityi

2-hydroxyphytanoyl-CoA = pristanal + formyl-CoA.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
  • thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Pathwayi: fatty acid metabolism

This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631Thiamine pyrophosphateBy similarity
Metal bindingi458 – 4581MagnesiumBy similarity
Metal bindingi485 – 4851MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • fatty acid alpha-oxidation Source: HGNC
  • lipid metabolic process Source: MGI
  • protein oligomerization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

ReactomeiR-MMU-389599. Alpha-oxidation of phytanate.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxyacyl-CoA lyase 1 (EC:4.1.-.-)
Alternative name(s):
2-hydroxyphytanoyl-CoA lyase
Short name:
2-HPCL
Phytanoyl-CoA 2-hydroxylase 2
Gene namesi
Name:Hacl1
Synonyms:Hpcl, Phyh2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1929657. Hacl1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5815812-hydroxyacyl-CoA lyase 1PRO_0000090817Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41PhosphoserineBy similarity
Modified residuei6 – 61PhosphoserineBy similarity
Modified residuei354 – 3541N6-succinyllysineCombined sources
Modified residuei361 – 3611N6-succinyllysineCombined sources
Modified residuei368 – 3681N6-succinyllysineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9QXE0.
MaxQBiQ9QXE0.
PaxDbiQ9QXE0.
PRIDEiQ9QXE0.

PTM databases

iPTMnetiQ9QXE0.
PhosphoSiteiQ9QXE0.
SwissPalmiQ9QXE0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021884.
CleanExiMM_HACL1.
ExpressionAtlasiQ9QXE0. baseline and differential.
GenevisibleiQ9QXE0. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9QXE0. 4 interactions.
MINTiMINT-1869780.
STRINGi10090.ENSMUSP00000022437.

Structurei

3D structure databases

ProteinModelPortaliQ9QXE0.
SMRiQ9QXE0. Positions 15-566.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni404 – 48784Thiamine pyrophosphate bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi579 – 5813Microbody targeting signalBy similarity

Sequence similaritiesi

Belongs to the TPP enzyme family.Curated

Phylogenomic databases

eggNOGiKOG1185. Eukaryota.
COG0028. LUCA.
GeneTreeiENSGT00530000063412.
HOGENOMiHOG000053808.
HOVERGENiHBG027302.
InParanoidiQ9QXE0.
KOiK12261.
OMAiFRHEQHA.
OrthoDBiEOG091G03OS.
PhylomeDBiQ9QXE0.
TreeFamiTF105690.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9QXE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPESNSAEGS DRSEEQVSGA KVIAQALKTQ DVEYMFGVVG IPVTEIALAA
60 70 80 90 100
QELGIKYIGM RNEQAACYAA SAVGYLTGRP GVCLVVSGPG LIHALGGMAN
110 120 130 140 150
ANMNCWPLIV IGGSSERNQE AMGAFQEFPQ VEACRLYTKF SARPSTIELI
160 170 180 190 200
PFIIEKAVRS SIYGRPGACY IDIPADFVTL QANVTSIKYK ECCMPPPVSM
210 220 230 240 250
AETSAVCAAA SVLRDAKQPL LIIGKGAAYS HAEDSIRKLV EQCSLPFLPT
260 270 280 290 300
PMGKGVVPDN HPNCVGAARS RALQSADVIV LFGARLNWIL HFGLPPRYQA
310 320 330 340 350
DVKFIQIDIC AEELGNNVRP SVILLGDIDA VSKQLLEQFD KTPWQCPPDS
360 370 380 390 400
QWWKTLREKM KSNEAISKEL ASQKSLPMNY YTVFYHVQEQ LPRDSFIVSE
410 420 430 440 450
GANTMDIGRT MLQNCLPRHR LDAGSFGTMG VGLGFAIAAA LVAKDRSPGQ
460 470 480 490 500
RVICVEGDSA FGFSGMEVET ICRYNLPIIL LVVNNNGIYQ GFDADTWEKM
510 520 530 540 550
LHFQEAATTV PPMCLLPNSH YEQVMTAFGG KGYFVRTPEE LQHSLRQALQ
560 570 580
DTSKPCLLNI MIEPQSTRKA QDFHWLTRSN M
Length:581
Mass (Da):63,660
Last modified:May 2, 2002 - v2
Checksum:i7CABBFC780A32A69
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti252 – 2521M → I in CAB65550 (PubMed:11171065).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132139 mRNA. Translation: CAB65550.1.
AK005505 mRNA. Translation: BAB24085.1.
AK041686 mRNA. Translation: BAC31032.1.
AK050078 mRNA. Translation: BAC34059.1.
BC021360 mRNA. Translation: AAH21360.1.
CCDSiCCDS26915.1.
RefSeqiNP_064359.2. NM_019975.3.
UniGeneiMm.38887.

Genome annotation databases

EnsembliENSMUST00000022437; ENSMUSP00000022437; ENSMUSG00000021884.
ENSMUST00000156431; ENSMUSP00000114922; ENSMUSG00000021884.
GeneIDi56794.
KEGGimmu:56794.
UCSCiuc007sxx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132139 mRNA. Translation: CAB65550.1.
AK005505 mRNA. Translation: BAB24085.1.
AK041686 mRNA. Translation: BAC31032.1.
AK050078 mRNA. Translation: BAC34059.1.
BC021360 mRNA. Translation: AAH21360.1.
CCDSiCCDS26915.1.
RefSeqiNP_064359.2. NM_019975.3.
UniGeneiMm.38887.

3D structure databases

ProteinModelPortaliQ9QXE0.
SMRiQ9QXE0. Positions 15-566.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QXE0. 4 interactions.
MINTiMINT-1869780.
STRINGi10090.ENSMUSP00000022437.

PTM databases

iPTMnetiQ9QXE0.
PhosphoSiteiQ9QXE0.
SwissPalmiQ9QXE0.

Proteomic databases

EPDiQ9QXE0.
MaxQBiQ9QXE0.
PaxDbiQ9QXE0.
PRIDEiQ9QXE0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022437; ENSMUSP00000022437; ENSMUSG00000021884.
ENSMUST00000156431; ENSMUSP00000114922; ENSMUSG00000021884.
GeneIDi56794.
KEGGimmu:56794.
UCSCiuc007sxx.2. mouse.

Organism-specific databases

CTDi26061.
MGIiMGI:1929657. Hacl1.

Phylogenomic databases

eggNOGiKOG1185. Eukaryota.
COG0028. LUCA.
GeneTreeiENSGT00530000063412.
HOGENOMiHOG000053808.
HOVERGENiHBG027302.
InParanoidiQ9QXE0.
KOiK12261.
OMAiFRHEQHA.
OrthoDBiEOG091G03OS.
PhylomeDBiQ9QXE0.
TreeFamiTF105690.

Enzyme and pathway databases

UniPathwayiUPA00199.
ReactomeiR-MMU-389599. Alpha-oxidation of phytanate.

Miscellaneous databases

PROiQ9QXE0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021884.
CleanExiMM_HACL1.
ExpressionAtlasiQ9QXE0. baseline and differential.
GenevisibleiQ9QXE0. MM.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 1 hit.
SSF52518. SSF52518. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiHACL1_MOUSE
AccessioniPrimary (citable) accession number: Q9QXE0
Secondary accession number(s): Q543K1, Q9DAV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 2, 2002
Last modified: September 7, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.