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Protein

SH3 and multiple ankyrin repeat domains protein 2

Gene

Shank2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.1 Publication

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-RNO-6794361 Neurexins and neuroligins

Names & Taxonomyi

Protein namesi
Recommended name:
SH3 and multiple ankyrin repeat domains protein 2
Short name:
Shank2
Alternative name(s):
Cortactin-binding protein 1
Short name:
CortBP1
GKAP/SAPAP-interacting protein
Proline-rich synapse-associated protein 1
Short name:
ProSAP1
SPANK-3
Gene namesi
Name:Shank2
Synonyms:Cortbp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi628772 Shank2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001746741 – 1474SH3 and multiple ankyrin repeat domains protein 2Add BLAST1474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei457PhosphoserineBy similarity1
Modified residuei486PhosphothreonineBy similarity1
Modified residuei586PhosphoserineBy similarity1
Modified residuei724PhosphoserineBy similarity1
Modified residuei903PhosphothreonineBy similarity1
Glycosylationi1292O-linked (GlcNAc) threonineBy similarity1
Modified residuei1334PhosphoserineBy similarity1
Modified residuei1338PhosphoserineBy similarity1
Isoform 4 (identifier: Q9QX74-4)
Modified residuei162PhosphoserineCombined sources1
Isoform 5 (identifier: Q9QX74-5)
Modified residuei162PhosphoserineCombined sources1
Isoform 6 (identifier: Q9QX74-6)
Modified residuei162PhosphoserineCombined sources1
Isoform 2 (identifier: Q9QX74-2)
Modified residuei373PhosphoserineCombined sources1
Isoform 3 (identifier: Q9QX74-3)
Modified residuei373PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9QX74
PRIDEiQ9QX74

PTM databases

iPTMnetiQ9QX74
PhosphoSitePlusiQ9QX74

Expressioni

Tissue specificityi

Expressed in epithelial cells (at protein level). All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).6 Publications

Developmental stagei

Expressed during early postnatal brain development, especially in the caudate putamen and thalamic nuclei. Expression in the cerebral cortex, the hippocampus and the cerebellum is moderate to high at P5 and shows a stable expression throughout development. Isoforms 1, 2, 4 and 6 are predominantly expressed in cerebellum up to the age of approximately 3 weeks. Isoform 1 expression decreases during development of cortex but slightly increases in cerebellum.1 Publication

Gene expression databases

BgeeiENSRNOG00000050206
ExpressionAtlasiQ9QX74 baseline and differential
GenevisibleiQ9QX74 RN

Interactioni

Subunit structurei

Is part of a complex with DLG4/PSD-95 and DLGAP1/GKAP. Interacts with CTTN/cortactin SH3 domain, DLGAP1/GKAP and alpha-latrotoxin receptor 1. Interacts with DNM2, DBNL, GRID2, BAIAP2, SLC9A3, PLCB3 and CFTR. Interacts with ABI1 (via SH3 domain). Interacts (via proline-rich region) with PDE4D isoform 5 (via N-terminal region). Interacts with PDE4D isoform 33, isoform 4, isoform 7, isoform 8 and isoform 9 but not isoform 32 and isoform 6. Interacts weakly with PDE4D isoform 31. Interacts with ABI1.9 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi251104, 2 interactors
CORUMiQ9QX74
IntActiQ9QX74, 11 interactors
MINTiQ9QX74
STRINGi10116.ENSRNOP00000067841

Structurei

3D structure databases

ProteinModelPortaliQ9QX74
SMRiQ9QX74
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini148 – 207SH3PROSITE-ProRule annotationAdd BLAST60
Domaini248 – 342PDZPROSITE-ProRule annotationAdd BLAST95
Domaini1411 – 1474SAMPROSITE-ProRule annotationAdd BLAST64

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1169 – 1175SH3-bindingSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi24 – 27Poly-Arg4
Compositional biasi521 – 529Poly-Pro9
Compositional biasi1203 – 1211Poly-Pro9

Domaini

The PDZ domain is required for interaction with GRID2, PLCB3, SLC9A3 and CFTR.

Sequence similaritiesi

Belongs to the SHANK family.Curated

Keywords - Domaini

SH3 domain, SH3-binding

Phylogenomic databases

eggNOGiKOG0504 Eukaryota
KOG4375 Eukaryota
COG0666 LUCA
GeneTreeiENSGT00510000046474
HOVERGENiHBG054027
InParanoidiQ9QX74
KOiK15009

Family and domain databases

InterProiView protein in InterPro
IPR001478 PDZ
IPR036034 PDZ_sf
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00595 PDZ, 1 hit
PF00536 SAM_1, 1 hit
PF07653 SH3_2, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00454 SAM, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF50044 SSF50044, 1 hit
SSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS50106 PDZ, 1 hit
PS50105 SAM_DOMAIN, 1 hit
PS50002 SH3, 1 hit

Sequences (7)i

Sequence statusi: Complete.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Note: So far detected are complete isoforms 2 to 5. Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: Q9QX74-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSLLNAFTK KEVPFREAPA YSNRRRRPPN TLAAPRVLLR SNSDNNLNAG
60 70 80 90 100
APEWAVCSAA TSHRSLSPQL LQQTPSKPDG ATKSLGSYAP GPRSRSPSLN
110 120 130 140 150
RLGGAGEDGK RPQPPHWHVG SPFTPGANKD SLSTFEYPGP RRKLYSAVPG
160 170 180 190 200
RLFVAIKPYQ PQVDGEIPLH RGDRVKVLSI GEGGFWEGSA RGHIGWFPAE
210 220 230 240 250
CVEEVQCKPR DSQAETRADR SKKLFRHYTV GSYDSFDAAS DCIIEDKTVV
260 270 280 290 300
LQKKDNEGFG FVLRGAKADT PIEEFTPTPA FPALQYLESV DEGGVAWQAG
310 320 330 340 350
LRTGDFLIEV NNENVVKVGH RQVVNMIRQG GNHLVLKVVT VTRNLDPDDT
360 370 380 390 400
ARKKAPPPPK RAPTTALTLR SKSMTAELEE LGLSLVDKAS VRKKKDKPEE
410 420 430 440 450
IVPASKPSRT AENVAIESRV ATIKQRPTSR CFPAASDVNS VYERQGIAVM
460 470 480 490 500
TPTVPGSPKG PFLGLPRGTM RRQKSIDSRI FLSGITEEER QFLAPPMLKF
510 520 530 540 550
TRSLSMPDTS EDIPPPPQSV PPSPPPPSPT TYNCPRSPTP RVYGTIKPAF
560 570 580 590 600
NQNPVAKVPP ATRSDTVATM MREKGMFYRR ELDRFSLDSE DVYSRSPAPQ
610 620 630 640 650
AAFRTKRGQM PENPYSEVGK IASKAVYVPA KPARRKGMLV KQSNVEDSPE
660 670 680 690 700
KTCSIPIPTI IVKEPSTSSS GKSSQGSSME IDPQATEPGQ LRPDDSLTVS
710 720 730 740 750
SPFAAAIAGA VRDREKRLEA RRNSPAFLST DLGDEDVGLG PPAPRMQPSK
760 770 780 790 800
FPEEGGFGDE DETEQPLLPT PGAAPRELEN HFLGGGEAGA QGEAGGPLSS
810 820 830 840 850
TSKAKGPESG PAAALKSSSP ASPENYVHPL TGRLLDPSSP LALALSARDR
860 870 880 890 900
AMQESQQGHK GEAPKADLNK PLYIDTKMRP SVESGFPPVT RQNTRGPLRR
910 920 930 940 950
QETENKYETD LSKDRRADDK KNMLINIVDT AQQKSAGLLM VHTVDIPVAG
960 970 980 990 1000
PPLEEEEDRE DGDTKPDHSP STVPEGVPKT EGALQISAAP EPAAAPGRTI
1010 1020 1030 1040 1050
VAAGSVEEAV ILPFRIPPPP LASVDLDEDF LFTEPLPPPL EFANSFDIPD
1060 1070 1080 1090 1100
DRAASVPALA DLVKQKKSDT PQPPSLNSSQ PANSTDSKKP AGISNCLPSS
1110 1120 1130 1140 1150
FLPPPESFDA VTDSGIEEVD SRSSSDHHLE TTSTISTVSS ISTLSSEGGE
1160 1170 1180 1190 1200
SMDTCTVYAD GQAFVVDKPP VPPKPKMKPI VHKSNALYQD TLPEEDTDGF
1210 1220 1230 1240 1250
VIPPPAPPPP PGSAQAGVAK VIQPRTSKLW GDVTEVKSPI LSGPKANVIS
1260 1270 1280 1290 1300
ELNSILQQMN RGKSVKPGEG LELPVGAKSA NLAPRSPEVM STVSGTRSTT
1310 1320 1330 1340 1350
VTFTVRPGTS QPITLQSRPP DYESRTSGPR RAPSPVVSPT ELSKEILPTP
1360 1370 1380 1390 1400
PSAAAASPSP TLSDVFSLPS QSPAGDLFGL NPAGRSRSPS PSILQQPISN
1410 1420 1430 1440 1450
KPFTTKPVHL WTKPDVADWL ESLNLGEHKE TFMDNEIDGS HLPNLQKEDL
1460 1470
IDLGVTRVGH RMNIERALKQ LLDR
Length:1,474
Mass (Da):158,684
Last modified:July 26, 2002 - v2
Checksum:iF503D44D0D9AB7C1
GO
Isoform 2 (identifier: Q9QX74-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     382-385: Missing.

Show »
Length:1,470
Mass (Da):158,313
Checksum:i3243014B3C11A193
GO
Isoform 3 (identifier: Q9QX74-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     382-385: Missing.
     388-394: Missing.
     468-483: Missing.

Show »
Length:1,447
Mass (Da):155,638
Checksum:i6C88FEB1E3AAB067
GO
Isoform 4 (identifier: Q9QX74-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-211: Missing.
     212-239: SQAETRADRSKKLFRHYTVGSYDSFDAA → MMSVPGGGAATVMMTGYNNGRYPRNSLY
     382-385: Missing.

Show »
Length:1,259
Mass (Da):135,093
Checksum:i1C367EBC2AFCA2D2
GO
Isoform 5 (identifier: Q9QX74-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-211: Missing.
     212-239: SQAETRADRSKKLFRHYTVGSYDSFDAA → MMSVPGGGAATVMMTGYNNGRYPRNSLY
     382-385: Missing.
     388-394: Missing.

Show »
Length:1,252
Mass (Da):134,295
Checksum:iB636E33D36CFA29B
GO
Isoform 6 (identifier: Q9QX74-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-211: Missing.
     212-239: SQAETRADRSKKLFRHYTVGSYDSFDAA → MMSVPGGGAATVMMTGYNNGRYPRNSLY
     382-385: Missing.
     468-476: Missing.

Show »
Length:1,250
Mass (Da):134,035
Checksum:i39E4227E05938C9A
GO
Isoform 7 (identifier: Q9QX74-7) [UniParc]FASTAAdd to basket
Also known as: E

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MKSLLNAFTKKE → MPRSPTSSED...YIKNHKETDI
     382-395: Missing.

Note: Contains 6 ANK repeats at positions 196-226, 230-259, 263-293, 297-326, 330-359, 363-393.
Show »
Length:1,839
Mass (Da):199,855
Checksum:i0A44ABEE5F37FCBE
GO

Sequence cautioni

The sequence AAD42977 differs from that shown. Reason: Frameshift at positions 46 and 165.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35 – 41PRVLLRS → QKNLGAA (PubMed:10506216).Curated7
Sequence conflicti152L → F in AAF02497 (PubMed:14977424).Curated1
Sequence conflicti369L → V in AAC62226 (PubMed:9742101).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0060811 – 211Missing in isoform 4, isoform 5 and isoform 6. 2 PublicationsAdd BLAST211
Alternative sequenceiVSP_0200491 – 12MKSLL…FTKKE → MPRSPTSSEDEMAQSFSDYS VGSESDSSKEETIYDTIRAT TEKPGGVKMEDLQGNTLVIR VVIQDLQQTKCIRFNPDATV WVAKQRILCTLNQGLKDVLN YGLFQPASNGRDGKFLDEER LLREYPQPMGQGVPSLEFRY KKRVYKQSNLDEKQLARLHT KTNLKKFMDHTQHRSVEKLV KLLDRGLDPNFHDLETGETP LTLAAQLDGSMEVIKALRNG GAHLDFRSRDGMTALHKAAR MRNQVALKTLLELGASPDYK DSYGLTPLYHTAIVGGDPYC CELLLHEHASVCCKDENGWH EIHQACRYGHVQHLEHLLFY GADMSAQNASGNTALHICAL YNQDSCARVLLFRGGDKELK NYNSQTPFQVAIIAGNFELA EYIKNHKETDI in isoform 7. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_006082212 – 239SQAET…SFDAA → MMSVPGGGAATVMMTGYNNG RYPRNSLY in isoform 4, isoform 5 and isoform 6. 2 PublicationsAdd BLAST28
Alternative sequenceiVSP_020050382 – 395Missing in isoform 7. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_006083382 – 385Missing in isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6. 3 Publications4
Alternative sequenceiVSP_006084388 – 394Missing in isoform 3 and isoform 5. 3 Publications7
Alternative sequenceiVSP_006086468 – 483Missing in isoform 3. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_006085468 – 476Missing in isoform 6. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF060116 mRNA Translation: AAC62226.1
AJ249562 mRNA Translation: CAB56522.1
AJ131899 mRNA Translation: CAB44314.1
AJ131899 mRNA Translation: CAB44312.1
AJ131899 mRNA Translation: CAB44313.1
AY298755 mRNA Translation: AAP85236.1
AF141903 mRNA Translation: AAF02497.1
AF159048 mRNA Translation: AAD42977.1 Frameshift.
PIRiT14272
RefSeqiNP_001004133.1, NM_001004133.1
NP_597684.1, NM_133440.1 [Q9QX74-4]
NP_597685.1, NM_133441.1 [Q9QX74-2]
NP_958738.1, NM_201350.1 [Q9QX74-7]
XP_017444213.1, XM_017588724.1 [Q9QX74-7]
UniGeneiRn.14545

Genome annotation databases

EnsembliENSRNOT00000071741; ENSRNOP00000067841; ENSRNOG00000050206 [Q9QX74-1]
ENSRNOT00000075078; ENSRNOP00000065891; ENSRNOG00000050206 [Q9QX74-7]
ENSRNOT00000092357; ENSRNOP00000075800; ENSRNOG00000050206 [Q9QX74-5]
ENSRNOT00000092386; ENSRNOP00000075919; ENSRNOG00000050206 [Q9QX74-4]
ENSRNOT00000092516; ENSRNOP00000075839; ENSRNOG00000050206 [Q9QX74-2]
GeneIDi171093
KEGGirno:171093
UCSCiRGD:628772 rat [Q9QX74-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSHAN2_RAT
AccessioniPrimary (citable) accession number: Q9QX74
Secondary accession number(s): O70470
, Q6WB19, Q9QX93, Q9QZZ9, Q9WUV9, Q9WUW0, Q9WV46
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: July 26, 2002
Last modified: May 23, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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