Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Deoxyguanosine kinase, mitochondrial

Gene

Dguok

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the phosphorylation of several deoxyribonucleosides and certain nucleoside analogs widely employed as antiviral and chemotherapeutic agents.By similarity

Catalytic activityi

ATP + deoxyguanosine = ADP + dGMP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701SubstrateBy similarity
Binding sitei100 – 1001SubstrateBy similarity
Binding sitei111 – 1111SubstrateBy similarity
Binding sitei118 – 1181SubstrateBy similarity
Active sitei141 – 1411Proton acceptorSequence Analysis
Binding sitei142 – 1421SubstrateBy similarity
Binding sitei147 – 1471SubstrateBy similarity
Binding sitei211 – 2111SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 539ATPBy similarity
Nucleotide bindingi206 – 2083ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.113. 3474.
ReactomeiREACT_345398. Purine salvage.
SABIO-RKQ9QX60.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyguanosine kinase, mitochondrial (EC:2.7.1.113)
Short name:
dGK
Gene namesi
Name:Dguok
Synonyms:Dgk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1351602. Dguok.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • mitochondrion Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939MitochondrionSequence AnalysisAdd
BLAST
Chaini40 – 277238Deoxyguanosine kinase, mitochondrialPRO_0000016841Add
BLAST

Proteomic databases

MaxQBiQ9QX60.
PaxDbiQ9QX60.
PRIDEiQ9QX60.

PTM databases

PhosphoSiteiQ9QX60.

Expressioni

Gene expression databases

CleanExiMM_DGUOK.
ExpressionAtlasiQ9QX60. baseline and differential.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ9QX60. 1 interaction.
MINTiMINT-4114990.
STRINGi10090.ENSMUSP00000014698.

Structurei

3D structure databases

ProteinModelPortaliQ9QX60.
SMRiQ9QX60. Positions 37-277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DCK/DGK family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1428.
GeneTreeiENSGT00510000046588.
HOGENOMiHOG000290165.
HOVERGENiHBG006216.
InParanoidiQ9QX60.
OMAiLLTKTYP.
OrthoDBiEOG70PBZM.
TreeFamiTF324413.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10513. PTHR10513. 1 hit.
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QX60-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGRFLLRR LRASFRSPLR NALVDAPHAR AMHDGGGPRR LCIEGNIAVG
60 70 80 90 100
KSTFVKLLMK THPEWQVATE PIAEWQNIQA AGAQKDGTSK RLGNLLEMMY
110 120 130 140 150
QEPARWSYTF QTLSFMSRLK VQLEPIPGRL LQAEKSVRVF ERSVYSDRYI
160 170 180 190 200
FAKNLFENGS LSDIEWHIYQ DWHSFLLQEF ANRLLLHGFI YLQASPQVCM
210 220 230 240 250
ERLYQRDREE EKGIELAYLQ QLHSQHEDWF INKTTKLHFE ALQHVPVLVL
260 270
DVTEDFSENA ARQEELMGQV NTFMRNL
Length:277
Mass (Da):32,281
Last modified:October 3, 2012 - v3
Checksum:i021C3B3335510279
GO
Isoform 2 (identifier: Q9QX60-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Show »
Length:246
Mass (Da):28,808
Checksum:i0F9F79B219519A88
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 232AL → CF in AAF14342 (Ref. 2) Curated
Sequence conflicti74 – 741E → A in CAB43121 (PubMed:10455141).Curated
Sequence conflicti74 – 741E → A in CAB43122 (PubMed:10455141).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 3131Missing in isoform 2. CuratedVSP_003027Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133749 mRNA. Translation: CAB43121.1.
AJ133750 mRNA. Translation: CAB43122.1.
U90524 mRNA. Translation: AAF14342.1.
AK035281 mRNA. Translation: BAC29014.1.
AC090648 Genomic DNA. No translation available.
CH466523 Genomic DNA. Translation: EDK99082.1.
CCDSiCCDS20279.1. [Q9QX60-1]
RefSeqiNP_038792.2. NM_013764.2. [Q9QX60-1]
UniGeneiMm.42234.

Genome annotation databases

EnsembliENSMUST00000014698; ENSMUSP00000014698; ENSMUSG00000014554. [Q9QX60-1]
GeneIDi27369.
UCSCiuc009cnn.2. mouse. [Q9QX60-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ133749 mRNA. Translation: CAB43121.1.
AJ133750 mRNA. Translation: CAB43122.1.
U90524 mRNA. Translation: AAF14342.1.
AK035281 mRNA. Translation: BAC29014.1.
AC090648 Genomic DNA. No translation available.
CH466523 Genomic DNA. Translation: EDK99082.1.
CCDSiCCDS20279.1. [Q9QX60-1]
RefSeqiNP_038792.2. NM_013764.2. [Q9QX60-1]
UniGeneiMm.42234.

3D structure databases

ProteinModelPortaliQ9QX60.
SMRiQ9QX60. Positions 37-277.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QX60. 1 interaction.
MINTiMINT-4114990.
STRINGi10090.ENSMUSP00000014698.

PTM databases

PhosphoSiteiQ9QX60.

Proteomic databases

MaxQBiQ9QX60.
PaxDbiQ9QX60.
PRIDEiQ9QX60.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014698; ENSMUSP00000014698; ENSMUSG00000014554. [Q9QX60-1]
GeneIDi27369.
UCSCiuc009cnn.2. mouse. [Q9QX60-1]

Organism-specific databases

CTDi1716.
MGIiMGI:1351602. Dguok.

Phylogenomic databases

eggNOGiCOG1428.
GeneTreeiENSGT00510000046588.
HOGENOMiHOG000290165.
HOVERGENiHBG006216.
InParanoidiQ9QX60.
OMAiLLTKTYP.
OrthoDBiEOG70PBZM.
TreeFamiTF324413.

Enzyme and pathway databases

BRENDAi2.7.1.113. 3474.
ReactomeiREACT_345398. Purine salvage.
SABIO-RKQ9QX60.

Miscellaneous databases

NextBioi305266.
PROiQ9QX60.
SOURCEiSearch...

Gene expression databases

CleanExiMM_DGUOK.
ExpressionAtlasiQ9QX60. baseline and differential.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR002624. DNK.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10513. PTHR10513. 1 hit.
PfamiPF01712. dNK. 1 hit.
[Graphical view]
PIRSFiPIRSF000705. DNK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of mouse deoxyguanosine kinase. Evidence for a cytoplasmic isoform."
    Petrakis T.G., Ktistaki E., Wang L., Eriksson S., Talianidis I.
    J. Biol. Chem. 274:24726-24730(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Spleen.
  2. "Cloning of mouse deoxyguanosine kinase cDNA."
    Johansson M., Holmdahl L., Karlsson A.
    Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Urinary bladder.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDGUOK_MOUSE
AccessioniPrimary (citable) accession number: Q9QX60
Secondary accession number(s): Q8CBU2, Q9R0Y2, Q9WUT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 3, 2012
Last modified: July 22, 2015
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.