Reviewed, UniProtKB/Swiss-Prot Q9QX22 (TPMT_MUSSP)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:

Tpmt

Organism

Mus spretus (Western wild mouse)

Taxonomic identifier

10096 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Thiopurine S-methyltransferase		PRO_0000220106
Sites
	Binding site	28	1	S-adenosyl-L-methionine By similarity
	Binding site	64	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	85	1	S-adenosyl-L-methionine By similarity
	Binding site	147	1	S-adenosyl-L-methionine By similarity
Amino acid modifications
	Modified residue	53	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QX22-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 860A0DC5BD6FBA4C	FASTA	240	27,618
	10 20 30 40 50 60 MSLDMKEHPD AEVQKNRVLT LEDWKDKWVT RHISFHQEQG HQLLKKHLDT FLKGQSGLRV 70 80 90 100 110 120 FFPLCGKAVE MKWFADRGHT VVGVEISEIG IREFFAEQNL SYTEEPLAEI AGAKVFKSSS 130 140 150 160 170 180 GSISLYCCSI FDLPRANIGK FDRIWDRGAL VAINPGDHDR YADIILSLLR KEFQYLMAVL 190 200 210 220 230 240 SYDPTKHAGP PFYVPSAELK RLFGTKCSMQ CLEEVDALEE RHKAWGLDYL FEKLYLLTEK

References

[1]	"Mouse thiopurine methyltransferase pharmacogenetics: cDNA cloning and characterization and processed pseudogene cloning." Adjei A.A., Johnson G.B., Otterness D.M., Weinshilboum R.M. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: EI.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF037045 mRNA. Translation: AAD02094.1.
UniGene	Mm.10169
3D structure databases
SMR	Q9QX22. Positions 9-240.
ModBase	Search...
Organism-specific databases
MGI	MGI:98812. Tpmt.
Phylogenomic databases
HOVERGEN	Q9QX22.
Enzyme and pathway databases
BRENDA	2.1.1.67. 291401.
Family and domain databases
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_MUSSP

Accession

Primary (citable) accession number: Q9QX22

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)