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Protein

Cytohesin-1

Gene

Cyth1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1 and ARF5 (PubMed:18042453). Promotes the activation of ARF factors through replacement of GDP with GTP (PubMed:18042453). Plays a role in the epithelial polarization (PubMed:20080746).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei281Phosphatidylinositol 3,4,5-trisphosphateBy similarity1
Binding sitei292Phosphatidylinositol 3,4,5-trisphosphateBy similarity1
Binding sitei302Phosphatidylinositol 3,4,5-trisphosphateBy similarity1
Binding sitei351Phosphatidylinositol 3,4,5-trisphosphateBy similarity1

GO - Molecular functioni

  • ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
  • lipid binding Source: UniProtKB-KW

GO - Biological processi

  • establishment of epithelial cell polarity Source: MGI
  • regulation of ARF protein signal transduction Source: InterPro

Keywordsi

Molecular functionGuanine-nucleotide releasing factor
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-6811438. Intra-Golgi traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytohesin-1
Alternative name(s):
PH, SEC7 and coiled-coil domain-containing protein 1
Short name:
CLM1
SEC7 homolog A
Short name:
mSec7-1
Gene namesi
Name:Cyth1
Synonyms:Pscd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1334257. Cyth1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Tight junction

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi157E → K: Impairs epithelium polarity. 1 Publication1
Mutagenesisi385L → A: Impairs autoinhibition; when associated with A-389. Impairs translocation to the cell membrane. 1 Publication1
Mutagenesisi389K → A: Impairs autoinhibition; when associated with A-385. Impairs translocation to the cell membrane. 1 Publication1
Mutagenesisi394S → E: Increases guanine exchange factor activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001201951 – 398Cytohesin-1Add BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9QX11.
PRIDEiQ9QX11.

PTM databases

iPTMnetiQ9QX11.
PhosphoSitePlusiQ9QX11.

Expressioni

Gene expression databases

BgeeiENSMUSG00000017132.
ExpressionAtlasiQ9QX11. baseline and differential.
GenevisibleiQ9QX11. MM.

Interactioni

Subunit structurei

Interacts with TRIM23 and CYTIP (By similarity). Interacts (via coiled-coil domain) with FRMD4A (via coiled-coil domain)(PubMed:20080746). Interacts with FRMD4B (PubMed:20080746). Found in a complex with PARD3, CYTH1 and FRMD4A (PubMed:20080746).By similarity1 Publication

Protein-protein interaction databases

IntActiQ9QX11. 1 interactor.
STRINGi10090.ENSMUSP00000101909.

Structurei

3D structure databases

ProteinModelPortaliQ9QX11.
SMRiQ9QX11.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 202SEC7PROSITE-ProRule annotationAdd BLAST130
Domaini260 – 377PHPROSITE-ProRule annotationAdd BLAST118

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 60Necessary for localization at adherens junction1 PublicationAdd BLAST60
Regioni269 – 277Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity9
Regioni388 – 396C-terminal autoinhibitory regionBy similarity9

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili10 – 67Sequence analysisAdd BLAST58

Domaini

Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate.By similarity
Autoinhibited by its C-terminal basic region.1 Publication

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0930. Eukaryota.
COG5307. LUCA.
GeneTreeiENSGT00760000119036.
HOVERGENiHBG002647.
InParanoidiQ9QX11.
KOiK18441.
TreeFamiTF352091.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProiView protein in InterPro
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
PfamiView protein in Pfam
PF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
SUPFAMiSSF48425. SSF48425. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiView protein in PROSITE
PS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QX11-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDDDSYVPS DLTAEERQEL ENIRRRKQEL LADIQRLKEE IAEVANEIES
60 70 80 90 100
LGSTEERKNM QRNKQVAMGR KKFNMDPKKG IQFLIENGLL KNTCEDIAQF
110 120 130 140 150
LYKGEGLNKT AIGDYLGERD EFSIQVLHAF VELHEFTDLN LVQALRQFLW
160 170 180 190 200
SFRLPGEAQK IDRMMEAFAQ RYCQCNTGVF QSTDTCYVLS FAIIMLNTSL
210 220 230 240 250
HNPNVKDKPT VERFIAMNRG INDGGDLPEE LLRNLYESIK NEPFKIPEDD
260 270 280 290 300
GNDLTHTFFN PDREGWLLKL GGGRVKTWKR RWFILTDNCL YYFEYTTDKE
310 320 330 340 350
PRGIIPLENL SIREVEDSKK PNCFELYIPD NKDQVIKACK TEADGRVVEG
360 370 380 390
NHTVYRISAP TPEEKEDWIK CIKAAISRDP FYEMLAARKK KVSSTKRH
Length:398
Mass (Da):46,274
Last modified:July 27, 2011 - v2
Checksum:i79E06E2364282E85
GO
Isoform 2 (identifier: Q9QX11-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     273-273: Missing.

Show »
Length:397
Mass (Da):46,217
Checksum:i75F3183266EC695A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3D → E in AAC71694 (PubMed:9745014).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006035273Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013464 mRNA. Translation: BAA33428.1.
AF051337 mRNA. Translation: AAC71694.1.
AB035538 mRNA. Translation: BAB13509.1.
CCDSiCCDS48996.1. [Q9QX11-1]
CCDS48997.1. [Q9QX11-2]
RefSeqiNP_001106169.1. NM_001112699.1. [Q9QX11-2]
NP_035310.2. NM_011180.3. [Q9QX11-1]
UniGeneiMm.86413.

Genome annotation databases

EnsembliENSMUST00000017276; ENSMUSP00000017276; ENSMUSG00000017132. [Q9QX11-2]
ENSMUST00000106305; ENSMUSP00000101912; ENSMUSG00000017132. [Q9QX11-1]
GeneIDi19157.
KEGGimmu:19157.
UCSCiuc011yil.1. mouse. [Q9QX11-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCYH1_MOUSE
AccessioniPrimary (citable) accession number: Q9QX11
Secondary accession number(s): O88817, Q76MU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: September 27, 2017
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot