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Q9QX11 (CYH1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytohesin-1
Alternative name(s):
PH, SEC7 and coiled-coil domain-containing protein 1
Short name=CLM1
SEC7 homolog A
Short name=mSec7-1
Gene names
Name:Cyth1
Synonyms:Pscd1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF factors through replacement of GDP with GTP. Ref.4

Subunit structure

Interacts with TRIM23 and CYTIP By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein. Cytoplasmcytosol Ref.4.

Domain

Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate By similarity. Ref.4

Autoinhibited by its C-terminal basic region. Ref.4

Sequence similarities

Contains 1 PH domain.

Contains 1 SEC7 domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9QX11-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9QX11-2)

The sequence of this isoform differs from the canonical sequence as follows:
     273-273: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398Cytohesin-1
PRO_0000120195

Regions

Domain73 – 202130SEC7
Domain260 – 377118PH
Region269 – 2779Phosphatidylinositol 3,4,5-trisphosphate binding By similarity
Region388 – 3969C-terminal autoinhibitory region By similarity
Coiled coil10 – 6758 Potential

Sites

Binding site2811Phosphatidylinositol 3,4,5-trisphosphate By similarity
Binding site2921Phosphatidylinositol 3,4,5-trisphosphate By similarity
Binding site3021Phosphatidylinositol 3,4,5-trisphosphate By similarity
Binding site3511Phosphatidylinositol 3,4,5-trisphosphate By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Natural variations

Alternative sequence2731Missing in isoform 2.
VSP_006035

Experimental info

Mutagenesis3851L → A: Impairs autoinhibition; when associated with A-389. Impairs translocation to the cell membrane. Ref.4
Mutagenesis3891K → A: Impairs autoinhibition; when associated with A-385. Impairs translocation to the cell membrane. Ref.4
Mutagenesis3941S → E: Increases guanine exchange factor activity. Ref.4
Sequence conflict31D → E in AAC71694. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 79E06E2364282E85

FASTA39846,274
        10         20         30         40         50         60 
MEDDDSYVPS DLTAEERQEL ENIRRRKQEL LADIQRLKEE IAEVANEIES LGSTEERKNM 

        70         80         90        100        110        120 
QRNKQVAMGR KKFNMDPKKG IQFLIENGLL KNTCEDIAQF LYKGEGLNKT AIGDYLGERD 

       130        140        150        160        170        180 
EFSIQVLHAF VELHEFTDLN LVQALRQFLW SFRLPGEAQK IDRMMEAFAQ RYCQCNTGVF 

       190        200        210        220        230        240 
QSTDTCYVLS FAIIMLNTSL HNPNVKDKPT VERFIAMNRG INDGGDLPEE LLRNLYESIK 

       250        260        270        280        290        300 
NEPFKIPEDD GNDLTHTFFN PDREGWLLKL GGGRVKTWKR RWFILTDNCL YYFEYTTDKE 

       310        320        330        340        350        360 
PRGIIPLENL SIREVEDSKK PNCFELYIPD NKDQVIKACK TEADGRVVEG NHTVYRISAP 

       370        380        390 
TPEEKEDWIK CIKAAISRDP FYEMLAARKK KVSSTKRH 

« Hide

Isoform 2 [UniParc].

Checksum: 75F3183266EC695A
Show »

FASTA39746,217

References

[1]"Complex regulation of multiple cytohesin-like genes in murine tissues and cells."
Kim H.-S., Chen Y., Lonai P.
FEBS Lett. 433:312-316(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Brain.
[2]"Cloning and sequencing of cDNA encoding mouse cytohesin-1."
O'Rourke A.M., Escuro G., Feeney A.J.
Immunogenetics 48:354-355(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: B10D2/NSNJ.
Tissue: Lymph node.
[3]"Cloning and characterization of the promoter of murine cytohesin-1 gene."
Goda N., Tanoue A., Kikuchi S., Tsujimoto G.
Biochim. Biophys. Acta 1493:195-199(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"Structural basis and mechanism of autoregulation in 3-phosphoinositide-dependent Grp1 family Arf GTPase exchange factors."
DiNitto J.P., Delprato A., Gabe Lee M.T., Cronin T.C., Huang S., Guilherme A., Czech M.P., Lambright D.G.
Mol. Cell 28:569-583(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AUTOINHIBITION, MUTAGENESIS OF LEU-385; LYS-389 AND SER-394.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB013464 mRNA. Translation: BAA33428.1.
AF051337 mRNA. Translation: AAC71694.1.
AB035538 mRNA. Translation: BAB13509.1.
CCDSCCDS48996.1. [Q9QX11-1]
CCDS48997.1. [Q9QX11-2]
RefSeqNP_001106169.1. NM_001112699.1. [Q9QX11-2]
NP_035310.2. NM_011180.3. [Q9QX11-1]
XP_006532583.1. XM_006532520.1. [Q9QX11-1]
UniGeneMm.86413.

3D structure databases

ProteinModelPortalQ9QX11.
SMRQ9QX11. Positions 58-391.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9QX11. 1 interaction.

PTM databases

PhosphoSiteQ9QX11.

Proteomic databases

MaxQBQ9QX11.
PaxDbQ9QX11.
PRIDEQ9QX11.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000017276; ENSMUSP00000017276; ENSMUSG00000017132. [Q9QX11-2]
ENSMUST00000106305; ENSMUSP00000101912; ENSMUSG00000017132. [Q9QX11-1]
GeneID19157.
KEGGmmu:19157.
UCSCuc011yil.1. mouse. [Q9QX11-1]

Organism-specific databases

CTD9267.
MGIMGI:1334257. Cyth1.

Phylogenomic databases

eggNOGCOG5307.
GeneTreeENSGT00660000095162.
HOVERGENHBG002647.
TreeFamTF352091.

Gene expression databases

ArrayExpressQ9QX11.
BgeeQ9QX11.
GenevestigatorQ9QX11.

Family and domain databases

Gene3D1.10.1000.11. 1 hit.
2.30.29.30. 1 hit.
InterProIPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR023394. Sec7_alpha_orthog.
IPR000904. Sec7_dom.
[Graphical view]
PfamPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMSSF48425. SSF48425. 1 hit.
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCYTH1. mouse.
NextBio295810.
PROQ9QX11.
SOURCESearch...

Entry information

Entry nameCYH1_MOUSE
AccessionPrimary (citable) accession number: Q9QX11
Secondary accession number(s): O88817, Q76MU4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot