Q9QX11 (CYH1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytohesin-1

Alternative name(s):
PH, SEC7 and coiled-coil domain-containing protein 1
Short name=CLM1
SEC7 homolog A
Short name=mSec7-1

Gene names

Name:	Cyth1
Synonyms:	Pscd1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP.
Subunit structure	Interacts with TRIM23 and CYTIP By similarity.
Subcellular location	Cell membrane By similarity.
Sequence similarities	Contains 1 PH domain. Contains 1 SEC7 domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Molecular function	Guanine-nucleotide releasing factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	establishment of epithelial cell polarity Inferred from mutant phenotype PubMed 20080746. Source: MGI regulation of ARF protein signal transduction Inferred from electronic annotation. Source: InterPro regulation of cell adhesion Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB tight junction Inferred from direct assay PubMed 20080746. Source: MGI
Molecular_function	ARF guanyl-nucleotide exchange factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9QX11-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9QX11-2)

The sequence of this isoform differs from the canonical sequence as follows:
     273-273: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Cytohesin-1		PRO_0000120195
Regions
	Domain	73 – 202	130	SEC7
	Domain	260 – 377	118	PH
	Coiled coil	10 – 67	58	Potential
Natural variations
	Alternative sequence	273	1	Missing in isoform 2.		VSP_006035
Experimental info
	Sequence conflict	3	1	D → E in AAC71694. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 79E06E2364282E85	FASTA	398	46,274
	10 20 30 40 50 60 MEDDDSYVPS DLTAEERQEL ENIRRRKQEL LADIQRLKEE IAEVANEIES LGSTEERKNM 70 80 90 100 110 120 QRNKQVAMGR KKFNMDPKKG IQFLIENGLL KNTCEDIAQF LYKGEGLNKT AIGDYLGERD 130 140 150 160 170 180 EFSIQVLHAF VELHEFTDLN LVQALRQFLW SFRLPGEAQK IDRMMEAFAQ RYCQCNTGVF 190 200 210 220 230 240 QSTDTCYVLS FAIIMLNTSL HNPNVKDKPT VERFIAMNRG INDGGDLPEE LLRNLYESIK 250 260 270 280 290 300 NEPFKIPEDD GNDLTHTFFN PDREGWLLKL GGGRVKTWKR RWFILTDNCL YYFEYTTDKE 310 320 330 340 350 360 PRGIIPLENL SIREVEDSKK PNCFELYIPD NKDQVIKACK TEADGRVVEG NHTVYRISAP 370 380 390 TPEEKEDWIK CIKAAISRDP FYEMLAARKK KVSSTKRH
	Isoform 2 [UniParc]. Checksum: 75F3183266EC695A	FASTA	397	46,217
	10 20 30 40 50 60 MEDDDSYVPS DLTAEERQEL ENIRRRKQEL LADIQRLKEE IAEVANEIES LGSTEERKNM 70 80 90 100 110 120 QRNKQVAMGR KKFNMDPKKG IQFLIENGLL KNTCEDIAQF LYKGEGLNKT AIGDYLGERD 130 140 150 160 170 180 EFSIQVLHAF VELHEFTDLN LVQALRQFLW SFRLPGEAQK IDRMMEAFAQ RYCQCNTGVF 190 200 210 220 230 240 QSTDTCYVLS FAIIMLNTSL HNPNVKDKPT VERFIAMNRG INDGGDLPEE LLRNLYESIK 250 260 270 280 290 300 NEPFKIPEDD GNDLTHTFFN PDREGWLLKL GGRVKTWKRR WFILTDNCLY YFEYTTDKEP 310 320 330 340 350 360 RGIIPLENLS IREVEDSKKP NCFELYIPDN KDQVIKACKT EADGRVVEGN HTVYRISAPT 370 380 390 PEEKEDWIKC IKAAISRDPF YEMLAARKKK VSSTKRH

References

[1]	"Complex regulation of multiple cytohesin-like genes in murine tissues and cells." Kim H.-S., Chen Y., Lonai P. FEBS Lett. 433:312-316(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"Cloning and sequencing of cDNA encoding mouse cytohesin-1." O'Rourke A.M., Escuro G., Feeney A.J. Immunogenetics 48:354-355(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: B10D2/NSNJ. Tissue: Lymph node.
[3]	"Cloning and characterization of the promoter of murine cytohesin-1 gene." Goda N., Tanoue A., Kikuchi S., Tsujimoto G. Biochim. Biophys. Acta 1493:195-199(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB013464 mRNA. Translation: BAA33428.1. AF051337 mRNA. Translation: AAC71694.1. AB035538 mRNA. Translation: BAB13509.1.
IPI	IPI00315867. IPI00752843.
RefSeq	NP_001106169.1. NM_001112699.1. NP_035310.2. NM_011180.3.
UniGene	Mm.86413.
3D structure databases
ProteinModelPortal	Q9QX11.
SMR	Q9QX11. Positions 58-391.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9QX11. 1 interaction.
PTM databases
PhosphoSite	Q9QX11.
Proteomic databases
PaxDb	Q9QX11.
PRIDE	Q9QX11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000017276; ENSMUSP00000017276; ENSMUSG00000017132. ENSMUST00000106305; ENSMUSP00000101912; ENSMUSG00000017132.
GeneID	19157.
KEGG	mmu:19157.
Organism-specific databases
CTD	9267.
MGI	MGI:1334257. Cyth1.
Phylogenomic databases
eggNOG	COG5307.
GeneTree	ENSGT00660000095162.
HOVERGEN	HBG002647.
Gene expression databases
ArrayExpress	Q9QX11.
Bgee	Q9QX11.
Genevestigator	Q9QX11.
GermOnline	ENSMUSG00000017132. Mus musculus.
Family and domain databases
Gene3D	1.10.1000.11. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR000904. Sec7. IPR023394. SEC7_alpha_orthog. [Graphical view]
Pfam	PF00169. PH. 1 hit. PF01369. Sec7. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00222. Sec7. 1 hit. [Graphical view]
SUPFAM	SSF48425. Sec7. 1 hit.
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50190. SEC7. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CYTH1. mouse.
NextBio	295810.
SOURCE	Search...

Entry information

Entry name

CYH1_MOUSE

Accession

Primary (citable) accession number: Q9QX11
Secondary accession number(s): O88817, Q76MU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program