Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9QX11

- CYH1_MOUSE

UniProt

Q9QX11 - CYH1_MOUSE

Protein

Cytohesin-1

Gene

Cyth1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF factors through replacement of GDP with GTP.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei281 – 2811Phosphatidylinositol 3,4,5-trisphosphateBy similarity
    Binding sitei292 – 2921Phosphatidylinositol 3,4,5-trisphosphateBy similarity
    Binding sitei302 – 3021Phosphatidylinositol 3,4,5-trisphosphateBy similarity
    Binding sitei351 – 3511Phosphatidylinositol 3,4,5-trisphosphateBy similarity

    GO - Molecular functioni

    1. ARF guanyl-nucleotide exchange factor activity Source: UniProtKB
    2. lipid binding Source: UniProtKB-KW
    3. protein binding Source: MGI

    GO - Biological processi

    1. establishment of epithelial cell polarity Source: MGI
    2. positive regulation of GTPase activity Source: GOC
    3. regulation of ARF protein signal transduction Source: InterPro
    4. regulation of cell adhesion Source: RefGenome

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytohesin-1
    Alternative name(s):
    PH, SEC7 and coiled-coil domain-containing protein 1
    Short name:
    CLM1
    SEC7 homolog A
    Short name:
    mSec7-1
    Gene namesi
    Name:Cyth1
    Synonyms:Pscd1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1334257. Cyth1.

    Subcellular locationi

    Cell membrane 1 Publication; Peripheral membrane protein 1 Publication. Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. cytosol Source: UniProtKB
    2. extrinsic component of cytoplasmic side of plasma membrane Source: UniProtKB
    3. plasma membrane Source: UniProtKB
    4. tight junction Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi385 – 3851L → A: Impairs autoinhibition; when associated with A-389. Impairs translocation to the cell membrane. 1 Publication
    Mutagenesisi389 – 3891K → A: Impairs autoinhibition; when associated with A-385. Impairs translocation to the cell membrane. 1 Publication
    Mutagenesisi394 – 3941S → E: Increases guanine exchange factor activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 398398Cytohesin-1PRO_0000120195Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ9QX11.
    PaxDbiQ9QX11.
    PRIDEiQ9QX11.

    PTM databases

    PhosphoSiteiQ9QX11.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9QX11.
    BgeeiQ9QX11.
    GenevestigatoriQ9QX11.

    Interactioni

    Subunit structurei

    Interacts with TRIM23 and CYTIP.By similarity

    Protein-protein interaction databases

    IntActiQ9QX11. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QX11.
    SMRiQ9QX11. Positions 58-391.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini73 – 202130SEC7PROSITE-ProRule annotationAdd
    BLAST
    Domaini260 – 377118PHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni269 – 2779Phosphatidylinositol 3,4,5-trisphosphate bindingBy similarity
    Regioni388 – 3969C-terminal autoinhibitory regionBy similarity

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili10 – 6758Sequence AnalysisAdd
    BLAST

    Domaini

    Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate.By similarity
    Autoinhibited by its C-terminal basic region.1 Publication

    Sequence similaritiesi

    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 SEC7 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5307.
    GeneTreeiENSGT00660000095162.
    HOVERGENiHBG002647.
    TreeFamiTF352091.

    Family and domain databases

    Gene3Di1.10.1000.11. 1 hit.
    2.30.29.30. 1 hit.
    InterProiIPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR023394. Sec7_alpha_orthog.
    IPR000904. Sec7_dom.
    [Graphical view]
    PfamiPF00169. PH. 1 hit.
    PF01369. Sec7. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00222. Sec7. 1 hit.
    [Graphical view]
    SUPFAMiSSF48425. SSF48425. 1 hit.
    PROSITEiPS50003. PH_DOMAIN. 1 hit.
    PS50190. SEC7. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9QX11-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEDDDSYVPS DLTAEERQEL ENIRRRKQEL LADIQRLKEE IAEVANEIES    50
    LGSTEERKNM QRNKQVAMGR KKFNMDPKKG IQFLIENGLL KNTCEDIAQF 100
    LYKGEGLNKT AIGDYLGERD EFSIQVLHAF VELHEFTDLN LVQALRQFLW 150
    SFRLPGEAQK IDRMMEAFAQ RYCQCNTGVF QSTDTCYVLS FAIIMLNTSL 200
    HNPNVKDKPT VERFIAMNRG INDGGDLPEE LLRNLYESIK NEPFKIPEDD 250
    GNDLTHTFFN PDREGWLLKL GGGRVKTWKR RWFILTDNCL YYFEYTTDKE 300
    PRGIIPLENL SIREVEDSKK PNCFELYIPD NKDQVIKACK TEADGRVVEG 350
    NHTVYRISAP TPEEKEDWIK CIKAAISRDP FYEMLAARKK KVSSTKRH 398
    Length:398
    Mass (Da):46,274
    Last modified:July 27, 2011 - v2
    Checksum:i79E06E2364282E85
    GO
    Isoform 2 (identifier: Q9QX11-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         273-273: Missing.

    Show »
    Length:397
    Mass (Da):46,217
    Checksum:i75F3183266EC695A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31D → E in AAC71694. (PubMed:9745014)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei273 – 2731Missing in isoform 2. 1 PublicationVSP_006035

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013464 mRNA. Translation: BAA33428.1.
    AF051337 mRNA. Translation: AAC71694.1.
    AB035538 mRNA. Translation: BAB13509.1.
    CCDSiCCDS48996.1. [Q9QX11-1]
    CCDS48997.1. [Q9QX11-2]
    RefSeqiNP_001106169.1. NM_001112699.1. [Q9QX11-2]
    NP_035310.2. NM_011180.3. [Q9QX11-1]
    XP_006532583.1. XM_006532520.1. [Q9QX11-1]
    UniGeneiMm.86413.

    Genome annotation databases

    EnsembliENSMUST00000017276; ENSMUSP00000017276; ENSMUSG00000017132. [Q9QX11-2]
    ENSMUST00000106305; ENSMUSP00000101912; ENSMUSG00000017132. [Q9QX11-1]
    GeneIDi19157.
    KEGGimmu:19157.
    UCSCiuc011yil.1. mouse. [Q9QX11-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013464 mRNA. Translation: BAA33428.1 .
    AF051337 mRNA. Translation: AAC71694.1 .
    AB035538 mRNA. Translation: BAB13509.1 .
    CCDSi CCDS48996.1. [Q9QX11-1 ]
    CCDS48997.1. [Q9QX11-2 ]
    RefSeqi NP_001106169.1. NM_001112699.1. [Q9QX11-2 ]
    NP_035310.2. NM_011180.3. [Q9QX11-1 ]
    XP_006532583.1. XM_006532520.1. [Q9QX11-1 ]
    UniGenei Mm.86413.

    3D structure databases

    ProteinModelPortali Q9QX11.
    SMRi Q9QX11. Positions 58-391.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9QX11. 1 interaction.

    PTM databases

    PhosphoSitei Q9QX11.

    Proteomic databases

    MaxQBi Q9QX11.
    PaxDbi Q9QX11.
    PRIDEi Q9QX11.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000017276 ; ENSMUSP00000017276 ; ENSMUSG00000017132 . [Q9QX11-2 ]
    ENSMUST00000106305 ; ENSMUSP00000101912 ; ENSMUSG00000017132 . [Q9QX11-1 ]
    GeneIDi 19157.
    KEGGi mmu:19157.
    UCSCi uc011yil.1. mouse. [Q9QX11-1 ]

    Organism-specific databases

    CTDi 9267.
    MGIi MGI:1334257. Cyth1.

    Phylogenomic databases

    eggNOGi COG5307.
    GeneTreei ENSGT00660000095162.
    HOVERGENi HBG002647.
    TreeFami TF352091.

    Miscellaneous databases

    ChiTaRSi CYTH1. mouse.
    NextBioi 295810.
    PROi Q9QX11.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9QX11.
    Bgeei Q9QX11.
    Genevestigatori Q9QX11.

    Family and domain databases

    Gene3Di 1.10.1000.11. 1 hit.
    2.30.29.30. 1 hit.
    InterProi IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR023394. Sec7_alpha_orthog.
    IPR000904. Sec7_dom.
    [Graphical view ]
    Pfami PF00169. PH. 1 hit.
    PF01369. Sec7. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00222. Sec7. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48425. SSF48425. 1 hit.
    PROSITEi PS50003. PH_DOMAIN. 1 hit.
    PS50190. SEC7. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complex regulation of multiple cytohesin-like genes in murine tissues and cells."
      Kim H.-S., Chen Y., Lonai P.
      FEBS Lett. 433:312-316(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Tissue: Brain.
    2. "Cloning and sequencing of cDNA encoding mouse cytohesin-1."
      O'Rourke A.M., Escuro G., Feeney A.J.
      Immunogenetics 48:354-355(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: B10D2/NSNJ.
      Tissue: Lymph node.
    3. "Cloning and characterization of the promoter of murine cytohesin-1 gene."
      Goda N., Tanoue A., Kikuchi S., Tsujimoto G.
      Biochim. Biophys. Acta 1493:195-199(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    4. "Structural basis and mechanism of autoregulation in 3-phosphoinositide-dependent Grp1 family Arf GTPase exchange factors."
      DiNitto J.P., Delprato A., Gabe Lee M.T., Cronin T.C., Huang S., Guilherme A., Czech M.P., Lambright D.G.
      Mol. Cell 28:569-583(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, AUTOINHIBITION, MUTAGENESIS OF LEU-385; LYS-389 AND SER-394.

    Entry informationi

    Entry nameiCYH1_MOUSE
    AccessioniPrimary (citable) accession number: Q9QX11
    Secondary accession number(s): O88817, Q76MU4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3