ID   Q9QX10_RAT              Unreviewed;      1203 AA.
AC   Q9QX10;
DT   01-MAY-2000, integrated into UniProtKB/TrEMBL.
DT   01-MAY-2000, sequence version 1.
DT   27-MAR-2024, entry version 129.
DE   SubName: Full=Na-K-Cl cotransporter {ECO:0000313|EMBL:AAC27557.1};
GN   Name=Slc12a2 {ECO:0000313|RGD:620809};
GN   Synonyms=Nkcc1 {ECO:0000313|EMBL:AAC27557.1};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116 {ECO:0000313|EMBL:AAC27557.1};
RN   [1] {ECO:0000313|EMBL:AAC27557.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Wistar {ECO:0000313|EMBL:AAC27557.1};
RC   TISSUE=Parotid salivary gland {ECO:0000313|EMBL:AAC27557.1};
RX   PubMed=9733980; DOI=10.1016/S0005-2736(98)00112-6;
RA   Moore-Hoon M.L., Turner R.J.;
RT   "Molecular and topological characterization of the rat parotid Na+-K+-
RT   2Cl- cotransporter1.";
RL   Biochim. Biophys. Acta 1373:261-269(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 chloride(out) + K(+)(out) + Na(+)(out) = 2 chloride(in) +
CC         K(+)(in) + Na(+)(in); Xref=Rhea:RHEA:72395, ChEBI:CHEBI:17996,
CC         ChEBI:CHEBI:29101, ChEBI:CHEBI:29103;
CC         Evidence={ECO:0000256|ARBA:ARBA00034631};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72396;
CC         Evidence={ECO:0000256|ARBA:ARBA00034631};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC       {ECO:0000256|ARBA:ARBA00010593}.
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DR   EMBL; AF051561; AAC27557.1; -; mRNA.
DR   RefSeq; NP_113986.1; NM_031798.1.
DR   AlphaFoldDB; Q9QX10; -.
DR   iPTMnet; Q9QX10; -.
DR   PhosphoSitePlus; Q9QX10; -.
DR   GeneID; 83629; -.
DR   KEGG; rno:83629; -.
DR   AGR; RGD:620809; -.
DR   CTD; 6558; -.
DR   RGD; 620809; Slc12a2.
DR   OrthoDB; 5490251at2759; -.
DR   GO; GO:0016324; C:apical plasma membrane; ISO:RGD.
DR   GO; GO:0009925; C:basal plasma membrane; ISO:RGD.
DR   GO; GO:0016323; C:basolateral plasma membrane; ISO:RGD.
DR   GO; GO:0044297; C:cell body; ISO:RGD.
DR   GO; GO:0071944; C:cell periphery; ISO:RGD.
DR   GO; GO:0042995; C:cell projection; ISO:RGD.
DR   GO; GO:0030659; C:cytoplasmic vesicle membrane; ISO:RGD.
DR   GO; GO:0016328; C:lateral plasma membrane; ISO:RGD.
DR   GO; GO:0043005; C:neuron projection; ISO:RGD.
DR   GO; GO:0043025; C:neuronal cell body; ISO:RGD.
DR   GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR   GO; GO:0008519; F:ammonium transmembrane transporter activity; ISO:RGD.
DR   GO; GO:0015377; F:chloride:monoatomic cation symporter activity; ISO:RGD.
DR   GO; GO:0051879; F:Hsp90 protein binding; ISO:RGD.
DR   GO; GO:0046873; F:metal ion transmembrane transporter activity; ISO:RGD.
DR   GO; GO:0015079; F:potassium ion transmembrane transporter activity; ISO:RGD.
DR   GO; GO:0019901; F:protein kinase binding; ISO:RGD.
DR   GO; GO:0051087; F:protein-folding chaperone binding; ISO:RGD.
DR   GO; GO:0008511; F:sodium:potassium:chloride symporter activity; IMP:RGD.
DR   GO; GO:0072488; P:ammonium transmembrane transport; ISO:RGD.
DR   GO; GO:0060444; P:branching involved in mammary gland duct morphogenesis; ISO:RGD.
DR   GO; GO:0006884; P:cell volume homeostasis; IMP:ARUK-UCL.
DR   GO; GO:1990869; P:cellular response to chemokine; ISO:RGD.
DR   GO; GO:0071222; P:cellular response to lipopolysaccharide; ISO:RGD.
DR   GO; GO:0035865; P:cellular response to potassium ion; IMP:ARUK-UCL.
DR   GO; GO:0055064; P:chloride ion homeostasis; IBA:GO_Central.
DR   GO; GO:1902476; P:chloride transmembrane transport; ISO:RGD.
DR   GO; GO:0006821; P:chloride transport; IMP:RGD.
DR   GO; GO:0050910; P:detection of mechanical stimulus involved in sensory perception of sound; ISO:RGD.
DR   GO; GO:0007214; P:gamma-aminobutyric acid signaling pathway; IMP:ARUK-UCL.
DR   GO; GO:0006972; P:hyperosmotic response; IMP:RGD.
DR   GO; GO:0098658; P:inorganic anion import across plasma membrane; ISO:RGD.
DR   GO; GO:0098659; P:inorganic cation import across plasma membrane; ISO:RGD.
DR   GO; GO:0030644; P:intracellular chloride ion homeostasis; ISO:RGD.
DR   GO; GO:0030007; P:intracellular potassium ion homeostasis; ISO:RGD.
DR   GO; GO:0006883; P:intracellular sodium ion homeostasis; ISO:RGD.
DR   GO; GO:0035633; P:maintenance of blood-brain barrier; ISO:RGD.
DR   GO; GO:0060763; P:mammary duct terminal end bud growth; ISO:RGD.
DR   GO; GO:0035264; P:multicellular organism growth; ISO:RGD.
DR   GO; GO:0061044; P:negative regulation of vascular wound healing; ISO:RGD.
DR   GO; GO:1904450; P:positive regulation of aspartate secretion; ISO:RGD.
DR   GO; GO:0045795; P:positive regulation of cell volume; IMP:RGD.
DR   GO; GO:0055075; P:potassium ion homeostasis; IBA:GO_Central.
DR   GO; GO:1990573; P:potassium ion import across plasma membrane; ISO:RGD.
DR   GO; GO:0006813; P:potassium ion transport; IMP:RGD.
DR   GO; GO:1904464; P:regulation of matrix metallopeptidase secretion; ISO:RGD.
DR   GO; GO:0150003; P:regulation of spontaneous synaptic transmission; IMP:ARUK-UCL.
DR   GO; GO:0055078; P:sodium ion homeostasis; IBA:GO_Central.
DR   GO; GO:0098719; P:sodium ion import across plasma membrane; ISO:RGD.
DR   GO; GO:0035725; P:sodium ion transmembrane transport; IBA:GO_Central.
DR   GO; GO:0006814; P:sodium ion transport; IMP:RGD.
DR   GO; GO:0010818; P:T cell chemotaxis; ISO:RGD.
DR   GO; GO:0070634; P:transepithelial ammonium transport; ISO:RGD.
DR   GO; GO:0030321; P:transepithelial chloride transport; ISO:RGD.
DR   Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR   InterPro; IPR004841; AA-permease/SLC12A_dom.
DR   InterPro; IPR013612; AA_permease_N.
DR   InterPro; IPR002444; NKCC1.
DR   InterPro; IPR018491; SLC12_C.
DR   InterPro; IPR002443; SLC12A1/SLC12A2.
DR   InterPro; IPR004842; SLC12A_fam.
DR   NCBIfam; TIGR00930; 2a30; 1.
DR   PANTHER; PTHR11827:SF58; SOLUTE CARRIER FAMILY 12 MEMBER 2; 1.
DR   PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR   Pfam; PF00324; AA_permease; 1.
DR   Pfam; PF08403; AA_permease_N; 1.
DR   Pfam; PF03522; SLC12; 1.
DR   PRINTS; PR01207; NAKCLTRNSPRT.
DR   PRINTS; PR01208; NAKCLTRSPRT1.
DR   Genevisible; Q9QX10; RN.
PE   2: Evidence at transcript level;
KW   Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Sodium {ECO:0000256|ARBA:ARBA00023053};
KW   Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        308..334
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        355..379
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        399..418
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        427..448
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        478..500
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        512..532
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        590..613
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        646..666
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        672..690
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        702..721
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        727..742
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          193..244
FT                   /note="Amino acid permease N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF08403"
FT   DOMAIN          281..783
FT                   /note="Amino acid permease/ SLC12A"
FT                   /evidence="ECO:0000259|Pfam:PF00324"
FT   DOMAIN          792..1203
FT                   /note="SLC12A transporter C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF03522"
FT   REGION          1..102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          107..126
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          141..185
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          953..982
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        159..185
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        953..979
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1203 AA;  130352 MW;  F59E4959A7E760FC CRC64;
     MEPGPAAPSS GAPRPARDGD APLTAAGVDL PGTAVLSGRE DATAGSQAGG GVRGEGTPAA
     GDGLGKPLGP TPSQSRFQVD PVSENAGRAA AAAAAAAAAA AAGAAGKETP AAGKAGTESG
     VAKGSEEAKG RFRVNFVDPA ASSSADDSLS DAAGVGGDGP NVSSQNGGDT VLSEGSSLHS
     GGGSGHHQQY YYDTHTNTYY LRTFGHNTMD AVPRIDHYRH TAAQLGEKLL RPSLGEFHDE
     LEKEPFEDGF ANGEESTPTR DAVVTYTAES KGVVKFGWIK GVLVRCMLNI WGVMLFIRLS
     WIVGQAGIGL SVLVIAMATV VTTITGLSTS AIATNGFVRG GGAYYLISRS LGPEFGGAIG
     LIFAFPNAVA VAMYVVGFAE TVVELLKEHS ILMIDEINDI RIIGAITVVV LLGISVAGME
     WEAKAQIVLL VILLLAIADF VIGTFISLDS KKPKGFFGYK SEIFSENFGP DFREEETFFS
     VFAIFFPAAT GILAGANISG DLADPQSAIP KGTLLAILIT TVVYIGIAVS VGSCVVRDAT
     GNVNDTITTE LTNCTSAACK LNFDFSYCES NTCSYGLMNN FQVMSMVSGF APLISAGIFS
     ATLSSALASL VSAPKIFQAL CKDNIYPAFQ MFAKGYGKNN EPLRGYILTF LIALGFILIA
     ELNVIAPIIS NFFLASYALI NFSVFHASLA KSPGWRPAFK YYNMWISLTG AILCCIVMFV
     INWWAALLTY VIVLGLYIYV TYKKPDVNWG SSTQALTYLS ALQHSIRLSG VEDHVKNFRP
     QCLVMTGSPN SRPALLHLVH DFTKNVGLMI CGHVHMGPRR QAMKEMSIDQ AKYQRWLIKN
     KMKAFYAPVH ADDLREGAQY LMQAAGLGRM KPNTLVLGFK KDWLQADMRD VDMYINLFHD
     AFDIQYGVVV IRLKEGLDIS HLQGQEELLS SQEKSPGTKD VVVNVDYSKK SDQDAFKASG
     EKPITQKDEE EDGKTSTQPL LKKESKGPVA PLNVADQKLL EASTQFQKKQ GKNTIDVWWL
     FDDGGLTLLI PYLLTTKKKW KDCKIRVFIG GKINRIDHDR RAMATLLSKF RIDFSDIMVL
     GDINTKPKKE NIVAFDDMIE PYRLHEDDKE QDIADKMKED EPWRITDNEL ELYKTKTYRQ
     IRLNELLKEH SSTANIIVMS LPVARKGAVS SALYMAWLEA LSKDLPPILL VRGNHQSVLT
     FYS
//