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Q9QX05 (TLR4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Toll-like receptor 4

Short name=Toll4
Alternative name(s):
CD_antigen=CD284
Gene names
Name:Tlr4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response By similarity.

Subunit structure

Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4 By similarity. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Interacts with MLK4; this interaction leads to negative regulation of TLR4 signaling By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein By similarity.

Domain

The TIR domain mediates interaction with NOX4 By similarity.

Sequence similarities

Belongs to the Toll-like receptor family.

Contains 16 LRR (leucine-rich) repeats.

Contains 1 LRRCT domain.

Contains 1 TIR domain.

Ontologies

Keywords
   Biological processImmunity
Inflammatory response
Innate immunity
   Cellular componentMembrane
   DomainLeucine-rich repeat
Repeat
Signal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processMyD88-dependent toll-like receptor signaling pathway

Inferred from electronic annotation. Source: InterPro

cellular response to lipopolysaccharide

Inferred from expression pattern PubMed 20438866. Source: RGD

cellular response to mechanical stimulus

Inferred from expression pattern PubMed 20202224. Source: RGD

cellular response to retinoic acid

Inferred from expression pattern PubMed 20438866. Source: RGD

defense response to Gram-negative bacterium

Inferred from electronic annotation. Source: InterPro

detection of lipopolysaccharide

Inferred from sequence or structural similarity. Source: UniProtKB

immunoglobulin mediated immune response

Inferred from expression pattern PubMed 19257980. Source: RGD

inflammatory response

Inferred from expression pattern PubMed 15204789. Source: RGD

innate immune response

Traceable author statement PubMed 12616494. Source: RGD

innate immune response-activating signal transduction

Inferred from mutant phenotype PubMed 19226379. Source: RGD

leukotriene metabolic process

Inferred from direct assay PubMed 21765618. Source: RGD

microglial cell activation

Inferred from direct assay PubMed 16973131. Source: RGD

positive regulation of DNA binding

Inferred from mutant phenotype PubMed 16272348. Source: RGD

positive regulation of NF-kappaB transcription factor activity

Inferred from mutant phenotype PubMed 16651628. Source: RGD

positive regulation of apoptotic process

Inferred from mutant phenotype PubMed 16272348. Source: RGD

positive regulation of inflammatory response

Inferred from mutant phenotype PubMed 16651628. Source: RGD

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 12616494. Source: RGD

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from mutant phenotype PubMed 16272348. Source: RGD

regulation of cytokine secretion

Inferred from electronic annotation. Source: InterPro

regulation of sensory perception of pain

Inferred from mutant phenotype PubMed 20478276. Source: RGD

response to activity

Inferred from expression pattern PubMed 20007518PubMed 20369365. Source: RGD

response to ethanol

Inferred from mutant phenotype PubMed 16272348. Source: RGD

response to fatty acid

Inferred from direct assay PubMed 19144836. Source: RGD

response to gold nanoparticle

Inferred from expression pattern PubMed 23150627. Source: RGD

response to hypoxia

Inferred from expression pattern PubMed 15535030. Source: RGD

response to insulin

Inferred from expression pattern PubMed 16740310. Source: RGD

response to lipopolysaccharide

Inferred from direct assay PubMed 16973131. Source: RGD

response to morphine

Inferred from expression pattern PubMed 18599265. Source: RGD

response to oxidative stress

Inferred from mutant phenotype PubMed 16847070. Source: RGD

response to progesterone

Inferred from expression pattern PubMed 18316784. Source: RGD

response to retinoic acid

Inferred from expression pattern PubMed 20438866. Source: RGD

response to toxic substance

Inferred from expression pattern PubMed 15535030. Source: RGD

skeletal muscle contraction

Inferred from expression pattern PubMed 20007518. Source: RGD

toll-like receptor 4 signaling pathway

Inferred from electronic annotation. Source: InterPro

toll-like receptor signaling pathway

Inferred from direct assay PubMed 16973131. Source: RGD

   Cellular_componentcell surface

Inferred from direct assay PubMed 16847070. Source: RGD

cytoplasm

Inferred from direct assay PubMed 16847070. Source: RGD

integral component of plasma membrane

Traceable author statement PubMed 11994482. Source: RGD

lipopolysaccharide receptor complex

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionlipopolysaccharide binding

Inferred from direct assay PubMed 16973131. Source: RGD

lipopolysaccharide receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

phosphatidylinositol 3-kinase binding

Inferred from physical interaction PubMed 14962484. Source: RGD

receptor activity

Inferred from direct assay PubMed 12616494. Source: RGD

transmembrane signaling receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 835810Toll-like receptor 4
PRO_0000034728

Regions

Topological domain26 – 638613Extracellular Potential
Transmembrane639 – 65921Helical; Potential
Topological domain660 – 835176Cytoplasmic Potential
Repeat54 – 7522LRR 1
Repeat78 – 9922LRR 2
Repeat102 – 12322LRR 3
Repeat126 – 14722LRR 4
Repeat150 – 17122LRR 5
Repeat175 – 19824LRR 6
Repeat204 – 22421LRR 7
Repeat226 – 24722LRR 8
Repeat372 – 38110LRR 9
Repeat398 – 42023LRR 10
Repeat421 – 44222LRR 11
Repeat446 – 46722LRR 12
Repeat470 – 49122LRR 13
Repeat495 – 51622LRR 14
Repeat519 – 54022LRR 15
Repeat543 – 56422LRR 16
Domain576 – 62752LRRCT
Domain670 – 816147TIR

Amino acid modifications

Glycosylation341N-linked (GlcNAc...) Potential
Glycosylation431N-linked (GlcNAc...) Potential
Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation1721N-linked (GlcNAc...) Potential
Glycosylation2041N-linked (GlcNAc...) Potential
Glycosylation2371N-linked (GlcNAc...) Potential
Glycosylation2481N-linked (GlcNAc...) Potential
Glycosylation2811N-linked (GlcNAc...) Potential
Glycosylation3071N-linked (GlcNAc...) Potential
Glycosylation4921N-linked (GlcNAc...) Potential
Glycosylation4951N-linked (GlcNAc...) Potential
Glycosylation5241N-linked (GlcNAc...) Potential
Glycosylation5721N-linked (GlcNAc...) Potential
Glycosylation5751N-linked (GlcNAc...) Potential
Glycosylation6221N-linked (GlcNAc...) Potential
Disulfide bond28 ↔ 39 By similarity
Disulfide bond280 ↔ 304 By similarity
Disulfide bond388 ↔ 389 By similarity
Disulfide bond580 ↔ 606 By similarity
Disulfide bond582 ↔ 625 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9QX05 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: DF5E16A30851E3A0

FASTA83596,072
        10         20         30         40         50         60 
MMPLLHLAGT LIMALFLSCL RPGSLNPCIE VLPNITYQCM DQNLSKIPHD IPYSTKNLDL 

        70         80         90        100        110        120 
SFNPLKILRS YSFTNFSQLQ WLDLSRCEIE TIEDKAWHGL NQLSTLVLTG NPIKSFSPGS 

       130        140        150        160        170        180 
FSGLTNLENL VAVETKMTSL EGFHIGQLIS LKKLNVAHNL IHSFKLPEYF SNLTNLEHVD 

       190        200        210        220        230        240 
LSYNYIQTIS VKDLQFLREN PQVNLSLDLS LNPIDSIQAQ AFQGIRLHEL TLRSNFNSSN 

       250        260        270        280        290        300 
VLKMCLQNMT GLHVHRLILG EFKNERNLES FDRSVMEGLC NVSIDEFRLT YINHFSDDIY 

       310        320        330        340        350        360 
NLNCLANISA MSFTGVHIKH IADVPRHFKW QSLSIIRCHL KPFPKLSLPF LKSWTLTTNR 

       370        380        390        400        410        420 
EDISFGQLAL PSLRYLDLSR NAMSFRGCCS YSDFGTNNLK YLDLSFNGVI LMSANFMGLE 

       430        440        450        460        470        480 
ELEYLDFQHS TLKKVTEFSV FLSLEKLLYL DISYTNTKID FDGIFLGLIS LNTLKMAGNS 

       490        500        510        520        530        540 
FKDNTLSNVF TNTTNLTFLD LSKCQLEQIS RGVFDTLYRL QLLNMSHNNL LFLDPSHYKQ 

       550        560        570        580        590        600 
LYSLRTLDCS FNRIETSKGI LQHFPKSLAV FNLTNNSVAC ICEYQNFLQW VKDQKMFLVN 

       610        620        630        640        650        660 
VEQMKCASPI DMKASLVLDF TNSTCYIYKT IISVSVVSVL VVATVAFLIY HFYFHLILIA 

       670        680        690        700        710        720 
GCKKYSRGES IYDAFVIYSS QNEDWVRNEL VKNLEEGVPR FQLCLHYRDF IPGVAIAANI 

       730        740        750        760        770        780 
IQEGFHKSRK VIVVVSRHFI QSRWCIFEYE IAQTWQFLSS RSGIIFIVLE KVEKSLLRQQ 

       790        800        810        820        830 
VELYRLLSRN TYLEWEDNAL GRHIFWRRLK KALLDGKALN PDETSEEEQE ATTLT 

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References

[1]"Toll4 (TLR4) expression in cardiac myocytes in normal and failing myocardium."
Frantz S., Kobzik L., Kim Y.-D., Fukazawa R., Medzhitov R., Lee R.T., Kelly R.A.
J. Clin. Invest. 104:271-280(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Heart.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF057025 mRNA. Translation: AAC13313.1.
RefSeqNP_062051.1. NM_019178.1.
UniGeneRn.14534.

3D structure databases

ProteinModelPortalQ9QX05.
SMRQ9QX05. Positions 27-625.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ9QX05.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29260.
KEGGrno:29260.
UCSCRGD:3870. rat.

Organism-specific databases

CTD7099.
RGD3870. Tlr4.

Phylogenomic databases

eggNOGNOG249751.
HOGENOMHOG000037951.
HOVERGENHBG018823.
InParanoidQ9QX05.
KOK10160.
PhylomeDBQ9QX05.

Gene expression databases

GenevestigatorQ9QX05.

Family and domain databases

Gene3D3.40.50.10140. 1 hit.
InterProIPR000483. Cys-rich_flank_reg_C.
IPR001611. Leu-rich_rpt.
IPR000157. TIR_dom.
IPR027168. TLR4.
IPR017241. Toll-like_receptor.
[Graphical view]
PANTHERPTHR24365:SF9. PTHR24365:SF9. 1 hit.
PfamPF01582. TIR. 1 hit.
[Graphical view]
PIRSFPIRSF037595. Toll-like_receptor. 1 hit.
SMARTSM00082. LRRCT. 1 hit.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMSSF52200. SSF52200. 1 hit.
PROSITEPS51450. LRR. 13 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio608584.
PROQ9QX05.

Entry information

Entry nameTLR4_RAT
AccessionPrimary (citable) accession number: Q9QX05
Entry history
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families