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Q9QX05

- TLR4_RAT

UniProt

Q9QX05 - TLR4_RAT

Protein

Toll-like receptor 4

Gene

Tlr4

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids such as palmitate By similarity.By similarity

    GO - Molecular functioni

    1. lipopolysaccharide binding Source: RGD
    2. lipopolysaccharide receptor activity Source: UniProtKB
    3. phosphatidylinositol 3-kinase binding Source: RGD
    4. protein binding Source: RGD
    5. receptor activity Source: RGD
    6. transmembrane signaling receptor activity Source: InterPro

    GO - Biological processi

    1. cellular response to lipopolysaccharide Source: RGD
    2. cellular response to mechanical stimulus Source: RGD
    3. cellular response to retinoic acid Source: RGD
    4. defense response to Gram-negative bacterium Source: InterPro
    5. detection of lipopolysaccharide Source: UniProtKB
    6. immunoglobulin mediated immune response Source: RGD
    7. inflammatory response Source: RGD
    8. innate immune response Source: RGD
    9. innate immune response-activating signal transduction Source: RGD
    10. leukotriene metabolic process Source: RGD
    11. microglial cell activation Source: RGD
    12. MyD88-dependent toll-like receptor signaling pathway Source: InterPro
    13. positive regulation of apoptotic process Source: RGD
    14. positive regulation of DNA binding Source: RGD
    15. positive regulation of inflammatory response Source: RGD
    16. positive regulation of NF-kappaB transcription factor activity Source: RGD
    17. positive regulation of peptidyl-tyrosine phosphorylation Source: RGD
    18. positive regulation of sequence-specific DNA binding transcription factor activity Source: RGD
    19. regulation of cytokine secretion Source: InterPro
    20. regulation of sensory perception of pain Source: RGD
    21. response to activity Source: RGD
    22. response to ethanol Source: RGD
    23. response to fatty acid Source: RGD
    24. response to gold nanoparticle Source: RGD
    25. response to hypoxia Source: RGD
    26. response to insulin Source: RGD
    27. response to lipopolysaccharide Source: RGD
    28. response to morphine Source: RGD
    29. response to oxidative stress Source: RGD
    30. response to progesterone Source: RGD
    31. response to retinoic acid Source: RGD
    32. response to toxic substance Source: RGD
    33. skeletal muscle contraction Source: RGD
    34. toll-like receptor 4 signaling pathway Source: InterPro
    35. toll-like receptor signaling pathway Source: RGD

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Immunity, Inflammatory response, Innate immunity

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Toll-like receptor 4
    Short name:
    Toll4
    Alternative name(s):
    CD_antigen: CD284
    Gene namesi
    Name:Tlr4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3870. Tlr4.

    Subcellular locationi

    Membrane By similarity; Single-pass type I membrane protein By similarity

    GO - Cellular componenti

    1. cell surface Source: RGD
    2. cytoplasm Source: RGD
    3. integral component of plasma membrane Source: RGD
    4. lipopolysaccharide receptor complex Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 835810Toll-like receptor 4PRO_0000034728Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi28 ↔ 39By similarity
    Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi43 – 431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi204 – 2041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi237 – 2371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi280 ↔ 304By similarity
    Glycosylationi281 – 2811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi388 ↔ 389By similarity
    Glycosylationi492 – 4921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi495 – 4951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi524 – 5241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi572 – 5721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi575 – 5751N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi580 ↔ 606By similarity
    Disulfide bondi582 ↔ 625By similarity
    Glycosylationi622 – 6221N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ9QX05.

    Expressioni

    Gene expression databases

    GenevestigatoriQ9QX05.

    Interactioni

    Subunit structurei

    Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4 By similarity. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Interacts with MLK4; this interaction leads to negative regulation of TLR4 signaling By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QX05.
    SMRiQ9QX05. Positions 27-625.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini26 – 638613ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini660 – 835176CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei639 – 65921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati54 – 7522LRR 1Add
    BLAST
    Repeati78 – 9922LRR 2Add
    BLAST
    Repeati102 – 12322LRR 3Add
    BLAST
    Repeati126 – 14722LRR 4Add
    BLAST
    Repeati150 – 17122LRR 5Add
    BLAST
    Repeati175 – 19824LRR 6Add
    BLAST
    Repeati204 – 22421LRR 7Add
    BLAST
    Repeati226 – 24722LRR 8Add
    BLAST
    Repeati372 – 38110LRR 9
    Repeati398 – 42023LRR 10Add
    BLAST
    Repeati421 – 44222LRR 11Add
    BLAST
    Repeati446 – 46722LRR 12Add
    BLAST
    Repeati470 – 49122LRR 13Add
    BLAST
    Repeati495 – 51622LRR 14Add
    BLAST
    Repeati519 – 54022LRR 15Add
    BLAST
    Repeati543 – 56422LRR 16Add
    BLAST
    Domaini576 – 62752LRRCTAdd
    BLAST
    Domaini670 – 816147TIRPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The TIR domain mediates interaction with NOX4.By similarity

    Sequence similaritiesi

    Belongs to the Toll-like receptor family.Curated
    Contains 16 LRR (leucine-rich) repeats.Curated
    Contains 1 LRRCT domain.Curated
    Contains 1 TIR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249751.
    HOGENOMiHOG000037951.
    HOVERGENiHBG018823.
    InParanoidiQ9QX05.
    KOiK10160.
    PhylomeDBiQ9QX05.

    Family and domain databases

    Gene3Di3.40.50.10140. 1 hit.
    InterProiIPR000483. Cys-rich_flank_reg_C.
    IPR001611. Leu-rich_rpt.
    IPR000157. TIR_dom.
    IPR027168. TLR4.
    IPR017241. Toll-like_receptor.
    [Graphical view]
    PANTHERiPTHR24365:SF230. PTHR24365:SF230. 1 hit.
    PfamiPF13855. LRR_8. 3 hits.
    PF01582. TIR. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037595. Toll-like_receptor. 1 hit.
    SMARTiSM00082. LRRCT. 1 hit.
    SM00255. TIR. 1 hit.
    [Graphical view]
    SUPFAMiSSF52200. SSF52200. 1 hit.
    PROSITEiPS51450. LRR. 13 hits.
    PS50104. TIR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9QX05-1 [UniParc]FASTAAdd to Basket

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    MMPLLHLAGT LIMALFLSCL RPGSLNPCIE VLPNITYQCM DQNLSKIPHD    50
    IPYSTKNLDL SFNPLKILRS YSFTNFSQLQ WLDLSRCEIE TIEDKAWHGL 100
    NQLSTLVLTG NPIKSFSPGS FSGLTNLENL VAVETKMTSL EGFHIGQLIS 150
    LKKLNVAHNL IHSFKLPEYF SNLTNLEHVD LSYNYIQTIS VKDLQFLREN 200
    PQVNLSLDLS LNPIDSIQAQ AFQGIRLHEL TLRSNFNSSN VLKMCLQNMT 250
    GLHVHRLILG EFKNERNLES FDRSVMEGLC NVSIDEFRLT YINHFSDDIY 300
    NLNCLANISA MSFTGVHIKH IADVPRHFKW QSLSIIRCHL KPFPKLSLPF 350
    LKSWTLTTNR EDISFGQLAL PSLRYLDLSR NAMSFRGCCS YSDFGTNNLK 400
    YLDLSFNGVI LMSANFMGLE ELEYLDFQHS TLKKVTEFSV FLSLEKLLYL 450
    DISYTNTKID FDGIFLGLIS LNTLKMAGNS FKDNTLSNVF TNTTNLTFLD 500
    LSKCQLEQIS RGVFDTLYRL QLLNMSHNNL LFLDPSHYKQ LYSLRTLDCS 550
    FNRIETSKGI LQHFPKSLAV FNLTNNSVAC ICEYQNFLQW VKDQKMFLVN 600
    VEQMKCASPI DMKASLVLDF TNSTCYIYKT IISVSVVSVL VVATVAFLIY 650
    HFYFHLILIA GCKKYSRGES IYDAFVIYSS QNEDWVRNEL VKNLEEGVPR 700
    FQLCLHYRDF IPGVAIAANI IQEGFHKSRK VIVVVSRHFI QSRWCIFEYE 750
    IAQTWQFLSS RSGIIFIVLE KVEKSLLRQQ VELYRLLSRN TYLEWEDNAL 800
    GRHIFWRRLK KALLDGKALN PDETSEEEQE ATTLT 835
    Length:835
    Mass (Da):96,072
    Last modified:May 1, 2000 - v1
    Checksum:iDF5E16A30851E3A0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF057025 mRNA. Translation: AAC13313.1.
    RefSeqiNP_062051.1. NM_019178.1.
    UniGeneiRn.14534.

    Genome annotation databases

    GeneIDi29260.
    KEGGirno:29260.
    UCSCiRGD:3870. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF057025 mRNA. Translation: AAC13313.1 .
    RefSeqi NP_062051.1. NM_019178.1.
    UniGenei Rn.14534.

    3D structure databases

    ProteinModelPortali Q9QX05.
    SMRi Q9QX05. Positions 27-625.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9QX05.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 29260.
    KEGGi rno:29260.
    UCSCi RGD:3870. rat.

    Organism-specific databases

    CTDi 7099.
    RGDi 3870. Tlr4.

    Phylogenomic databases

    eggNOGi NOG249751.
    HOGENOMi HOG000037951.
    HOVERGENi HBG018823.
    InParanoidi Q9QX05.
    KOi K10160.
    PhylomeDBi Q9QX05.

    Miscellaneous databases

    NextBioi 608584.
    PROi Q9QX05.

    Gene expression databases

    Genevestigatori Q9QX05.

    Family and domain databases

    Gene3Di 3.40.50.10140. 1 hit.
    InterProi IPR000483. Cys-rich_flank_reg_C.
    IPR001611. Leu-rich_rpt.
    IPR000157. TIR_dom.
    IPR027168. TLR4.
    IPR017241. Toll-like_receptor.
    [Graphical view ]
    PANTHERi PTHR24365:SF230. PTHR24365:SF230. 1 hit.
    Pfami PF13855. LRR_8. 3 hits.
    PF01582. TIR. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037595. Toll-like_receptor. 1 hit.
    SMARTi SM00082. LRRCT. 1 hit.
    SM00255. TIR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52200. SSF52200. 1 hit.
    PROSITEi PS51450. LRR. 13 hits.
    PS50104. TIR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Toll4 (TLR4) expression in cardiac myocytes in normal and failing myocardium."
      Frantz S., Kobzik L., Kim Y.-D., Fukazawa R., Medzhitov R., Lee R.T., Kelly R.A.
      J. Clin. Invest. 104:271-280(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Heart.

    Entry informationi

    Entry nameiTLR4_RAT
    AccessioniPrimary (citable) accession number: Q9QX05
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 11, 2002
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3