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Protein

Cell cycle checkpoint protein RAD1

Gene

Rad1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity (By similarity).By similarity

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-5685938. HDR through Single Strand Annealing (SSA).
R-MMU-5693607. Processing of DNA double-strand break ends.
R-MMU-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-69473. G2/M DNA damage checkpoint.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell cycle checkpoint protein RAD1 (EC:3.1.11.2)
Short name:
mRAD1
Alternative name(s):
DNA repair exonuclease rad1 homolog
Rad1-like DNA damage checkpoint protein
Gene namesi
Name:Rad1
Synonyms:Rec1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1316678. Rad1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002250061 – 280Cell cycle checkpoint protein RAD1Add BLAST280

Proteomic databases

PaxDbiQ9QWZ1.
PeptideAtlasiQ9QWZ1.
PRIDEiQ9QWZ1.

PTM databases

PhosphoSitePlusiQ9QWZ1.

Expressioni

Tissue specificityi

Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level). Expressed in brain, testis, kidney, heart, liver and lung.3 Publications

Gene expression databases

BgeeiENSMUSG00000022248.
CleanExiMM_RAD1.
ExpressionAtlasiQ9QWZ1. baseline and differential.
GenevisibleiQ9QWZ1. MM.

Interactioni

Subunit structurei

Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD1 interacts with POLB, FEN1, HUS1, HUS1B, RAD9A and RAD9B. Interacts with DNAJC7 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022856.

Structurei

3D structure databases

ProteinModelPortaliQ9QWZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the rad1 family.Curated

Phylogenomic databases

eggNOGiKOG3194. Eukaryota.
ENOG410YHQU. LUCA.
GeneTreeiENSGT00500000044913.
HOGENOMiHOG000008015.
HOVERGENiHBG053058.
InParanoidiQ9QWZ1.
KOiK02830.
OMAiCKVSVRT.
OrthoDBiEOG091G0HS8.
PhylomeDBiQ9QWZ1.
TreeFamiTF101211.

Family and domain databases

InterProiIPR003011. Cell_cycle_checkpoint_Rad1.
IPR003021. Rad1_Rec1_Rad17.
[Graphical view]
PANTHERiPTHR10870. PTHR10870. 1 hit.
PfamiPF02144. Rad1. 1 hit.
[Graphical view]
PRINTSiPR01245. RAD1REC1.
PR01246. RAD1REPAIR.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QWZ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLLTQYNEE EYEQYCLVAS LDNVRNLSTV LKAIHFREHA TCFATKNGIK
60 70 80 90 100
VTVENAKCVQ ANAFIQADVF QEFVIQEESV TFRINLTILL DCLSIFGSSP
110 120 130 140 150
TPGTLTALRM CYQGYGHPLM LFLEEGGVVT VCKITTQEPE ETLDFDFCST
160 170 180 190 200
NVMNKIILQS EGLREAFSEL DMTGDVLQIT VSPDKPYFRL STFGNAGNSH
210 220 230 240 250
LDYPKDSDLV EAFHCDKTQV NRYKLSLLKP STKALALSCK VSIRTDNRGF
260 270 280
LSLQYMIRNE DGQICFVEYY CCPDEEVPES
Length:280
Mass (Da):31,610
Last modified:May 1, 2000 - v1
Checksum:iF9D6ED292C5D4361
GO
Isoform 2 (identifier: Q9QWZ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-280: YKLSLLKPST...CCPDEEVPES → ISDVKYSCKT...QWVNVVPVQA

Show »
Length:272
Mass (Da):30,565
Checksum:i36207A21BA05BCF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28S → F (PubMed:9878245).Curated1
Sequence conflicti28S → F (PubMed:9660799).Curated1
Sequence conflicti28S → F (Ref. 6) Curated1
Sequence conflicti28S → F (Ref. 7) Curated1
Sequence conflicti71Q → E in BAE28343 (PubMed:16141072).Curated1
Sequence conflicti118P → T in BAE40457 (PubMed:16141072).Curated1
Sequence conflicti198N → S in AAC95465 (PubMed:9828137).Curated1
Sequence conflicti216D → N in AAC27248 (PubMed:9705507).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017337223 – 280YKLSL…EVPES → ISDVKYSCKTWLQLPEGVGY DPLSGLEWTLSERDGEQEVA QWVNVVPVQA in isoform 2. 1 PublicationAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074718 mRNA. Translation: AAC98094.1.
AF073523 mRNA. Translation: AAC95465.1.
AF076842 mRNA. Translation: AAC95524.1.
AJ004976 mRNA. Translation: CAA06250.1.
AF038841 mRNA. Translation: AAC27248.1.
AF058394 mRNA. Translation: AAC14139.1.
AF084514 mRNA. Translation: AAC35551.1.
AK146533 mRNA. Translation: BAE27240.1.
AK148098 mRNA. Translation: BAE28343.1.
AK150466 mRNA. Translation: BAE29583.1.
AK168588 mRNA. Translation: BAE40457.1.
BC048693 mRNA. Translation: AAH48693.1.
CCDSiCCDS27379.1. [Q9QWZ1-1]
CCDS70618.1. [Q9QWZ1-2]
RefSeqiNP_001276376.1. NM_001289447.1. [Q9QWZ1-1]
NP_001276377.1. NM_001289448.1. [Q9QWZ1-2]
NP_035362.2. NM_011232.3. [Q9QWZ1-1]
UniGeneiMm.38376.

Genome annotation databases

EnsembliENSMUST00000022856; ENSMUSP00000022856; ENSMUSG00000022248. [Q9QWZ1-1]
ENSMUST00000100775; ENSMUSP00000098338; ENSMUSG00000022248. [Q9QWZ1-2]
GeneIDi19355.
KEGGimmu:19355.
UCSCiuc007vgh.2. mouse. [Q9QWZ1-1]
uc033gtc.1. mouse. [Q9QWZ1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074718 mRNA. Translation: AAC98094.1.
AF073523 mRNA. Translation: AAC95465.1.
AF076842 mRNA. Translation: AAC95524.1.
AJ004976 mRNA. Translation: CAA06250.1.
AF038841 mRNA. Translation: AAC27248.1.
AF058394 mRNA. Translation: AAC14139.1.
AF084514 mRNA. Translation: AAC35551.1.
AK146533 mRNA. Translation: BAE27240.1.
AK148098 mRNA. Translation: BAE28343.1.
AK150466 mRNA. Translation: BAE29583.1.
AK168588 mRNA. Translation: BAE40457.1.
BC048693 mRNA. Translation: AAH48693.1.
CCDSiCCDS27379.1. [Q9QWZ1-1]
CCDS70618.1. [Q9QWZ1-2]
RefSeqiNP_001276376.1. NM_001289447.1. [Q9QWZ1-1]
NP_001276377.1. NM_001289448.1. [Q9QWZ1-2]
NP_035362.2. NM_011232.3. [Q9QWZ1-1]
UniGeneiMm.38376.

3D structure databases

ProteinModelPortaliQ9QWZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022856.

PTM databases

PhosphoSitePlusiQ9QWZ1.

Proteomic databases

PaxDbiQ9QWZ1.
PeptideAtlasiQ9QWZ1.
PRIDEiQ9QWZ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022856; ENSMUSP00000022856; ENSMUSG00000022248. [Q9QWZ1-1]
ENSMUST00000100775; ENSMUSP00000098338; ENSMUSG00000022248. [Q9QWZ1-2]
GeneIDi19355.
KEGGimmu:19355.
UCSCiuc007vgh.2. mouse. [Q9QWZ1-1]
uc033gtc.1. mouse. [Q9QWZ1-2]

Organism-specific databases

CTDi5810.
MGIiMGI:1316678. Rad1.

Phylogenomic databases

eggNOGiKOG3194. Eukaryota.
ENOG410YHQU. LUCA.
GeneTreeiENSGT00500000044913.
HOGENOMiHOG000008015.
HOVERGENiHBG053058.
InParanoidiQ9QWZ1.
KOiK02830.
OMAiCKVSVRT.
OrthoDBiEOG091G0HS8.
PhylomeDBiQ9QWZ1.
TreeFamiTF101211.

Enzyme and pathway databases

ReactomeiR-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-5685938. HDR through Single Strand Annealing (SSA).
R-MMU-5693607. Processing of DNA double-strand break ends.
R-MMU-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-69473. G2/M DNA damage checkpoint.

Miscellaneous databases

PROiQ9QWZ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022248.
CleanExiMM_RAD1.
ExpressionAtlasiQ9QWZ1. baseline and differential.
GenevisibleiQ9QWZ1. MM.

Family and domain databases

InterProiIPR003011. Cell_cycle_checkpoint_Rad1.
IPR003021. Rad1_Rec1_Rad17.
[Graphical view]
PANTHERiPTHR10870. PTHR10870. 1 hit.
PfamiPF02144. Rad1. 1 hit.
[Graphical view]
PRINTSiPR01245. RAD1REC1.
PR01246. RAD1REPAIR.
ProtoNetiSearch...

Entry informationi

Entry nameiRAD1_MOUSE
AccessioniPrimary (citable) accession number: Q9QWZ1
Secondary accession number(s): O70452
, O88391, Q3TGU1, Q3UG66, Q9QWZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.