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Q9QWZ1

- RAD1_MOUSE

UniProt

Q9QWZ1 - RAD1_MOUSE

Protein

Cell cycle checkpoint protein RAD1

Gene

Rad1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity By similarity.By similarity

    Catalytic activityi

    Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

    GO - Molecular functioni

    1. 3'-5' exonuclease activity Source: UniProtKB
    2. exodeoxyribonuclease III activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular response to ionizing radiation Source: Ensembl
    2. DNA damage checkpoint Source: Ensembl
    3. DNA repair Source: UniProtKB-KW
    4. nucleic acid phosphodiester bond hydrolysis Source: GOC

    Keywords - Molecular functioni

    Exonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cell cycle checkpoint protein RAD1 (EC:3.1.11.2)
    Short name:
    mRAD1
    Alternative name(s):
    DNA repair exonuclease rad1 homolog
    Rad1-like DNA damage checkpoint protein
    Gene namesi
    Name:Rad1
    Synonyms:Rec1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1316678. Rad1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 280280Cell cycle checkpoint protein RAD1PRO_0000225006Add
    BLAST

    Proteomic databases

    PRIDEiQ9QWZ1.

    PTM databases

    PhosphoSiteiQ9QWZ1.

    Expressioni

    Tissue specificityi

    Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level). Expressed in brain, testis, kidney, heart, liver and lung.3 Publications

    Gene expression databases

    ArrayExpressiQ9QWZ1.
    BgeeiQ9QWZ1.
    CleanExiMM_RAD1.
    GenevestigatoriQ9QWZ1.

    Interactioni

    Subunit structurei

    Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD1 interacts with POLB, FEN1, HUS1, HUS1B, RAD9A and RAD9B. Interacts with DNAJC7 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QWZ1.
    SMRiQ9QWZ1. Positions 15-275.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the rad1 family.Curated

    Phylogenomic databases

    eggNOGiNOG324428.
    GeneTreeiENSGT00500000044913.
    HOGENOMiHOG000008015.
    HOVERGENiHBG053058.
    InParanoidiQ9QWZ1.
    KOiK02830.
    OMAiCKVSVRT.
    OrthoDBiEOG7N0C4P.
    PhylomeDBiQ9QWZ1.
    TreeFamiTF101211.

    Family and domain databases

    InterProiIPR003011. Cell_cycle_checkpoint_Rad1.
    IPR003021. Rad1_Rec1_Rad17.
    [Graphical view]
    PANTHERiPTHR10870. PTHR10870. 1 hit.
    PfamiPF02144. Rad1. 1 hit.
    [Graphical view]
    PRINTSiPR01245. RAD1REC1.
    PR01246. RAD1REPAIR.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9QWZ1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPLLTQYNEE EYEQYCLVAS LDNVRNLSTV LKAIHFREHA TCFATKNGIK    50
    VTVENAKCVQ ANAFIQADVF QEFVIQEESV TFRINLTILL DCLSIFGSSP 100
    TPGTLTALRM CYQGYGHPLM LFLEEGGVVT VCKITTQEPE ETLDFDFCST 150
    NVMNKIILQS EGLREAFSEL DMTGDVLQIT VSPDKPYFRL STFGNAGNSH 200
    LDYPKDSDLV EAFHCDKTQV NRYKLSLLKP STKALALSCK VSIRTDNRGF 250
    LSLQYMIRNE DGQICFVEYY CCPDEEVPES 280
    Length:280
    Mass (Da):31,610
    Last modified:May 1, 2000 - v1
    Checksum:iF9D6ED292C5D4361
    GO
    Isoform 2 (identifier: Q9QWZ1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         223-280: YKLSLLKPST...CCPDEEVPES → ISDVKYSCKT...QWVNVVPVQA

    Show »
    Length:272
    Mass (Da):30,565
    Checksum:i36207A21BA05BCF8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281S → F(PubMed:9878245)Curated
    Sequence conflicti28 – 281S → F(PubMed:9660799)Curated
    Sequence conflicti28 – 281S → F1 PublicationCurated
    Sequence conflicti28 – 281S → F1 PublicationCurated
    Sequence conflicti71 – 711Q → E in BAE28343. (PubMed:16141072)Curated
    Sequence conflicti118 – 1181P → T in BAE40457. (PubMed:16141072)Curated
    Sequence conflicti198 – 1981N → S in AAC95465. (PubMed:9828137)Curated
    Sequence conflicti216 – 2161D → N in AAC27248. (PubMed:9705507)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei223 – 28058YKLSL…EVPES → ISDVKYSCKTWLQLPEGVGY DPLSGLEWTLSERDGEQEVA QWVNVVPVQA in isoform 2. 1 PublicationVSP_017337Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF074718 mRNA. Translation: AAC98094.1.
    AF073523 mRNA. Translation: AAC95465.1.
    AF076842 mRNA. Translation: AAC95524.1.
    AJ004976 mRNA. Translation: CAA06250.1.
    AF038841 mRNA. Translation: AAC27248.1.
    AF058394 mRNA. Translation: AAC14139.1.
    AF084514 mRNA. Translation: AAC35551.1.
    AK146533 mRNA. Translation: BAE27240.1.
    AK148098 mRNA. Translation: BAE28343.1.
    AK150466 mRNA. Translation: BAE29583.1.
    AK168588 mRNA. Translation: BAE40457.1.
    BC048693 mRNA. Translation: AAH48693.1.
    CCDSiCCDS27379.1. [Q9QWZ1-1]
    CCDS70618.1. [Q9QWZ1-2]
    RefSeqiNP_001276376.1. NM_001289447.1. [Q9QWZ1-1]
    NP_001276377.1. NM_001289448.1. [Q9QWZ1-2]
    NP_035362.2. NM_011232.3. [Q9QWZ1-1]
    XP_006520102.1. XM_006520039.1. [Q9QWZ1-1]
    UniGeneiMm.38376.

    Genome annotation databases

    EnsembliENSMUST00000022856; ENSMUSP00000022856; ENSMUSG00000022248. [Q9QWZ1-1]
    ENSMUST00000100775; ENSMUSP00000098338; ENSMUSG00000022248. [Q9QWZ1-2]
    GeneIDi19355.
    KEGGimmu:19355.
    UCSCiuc007vgh.1. mouse. [Q9QWZ1-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF074718 mRNA. Translation: AAC98094.1 .
    AF073523 mRNA. Translation: AAC95465.1 .
    AF076842 mRNA. Translation: AAC95524.1 .
    AJ004976 mRNA. Translation: CAA06250.1 .
    AF038841 mRNA. Translation: AAC27248.1 .
    AF058394 mRNA. Translation: AAC14139.1 .
    AF084514 mRNA. Translation: AAC35551.1 .
    AK146533 mRNA. Translation: BAE27240.1 .
    AK148098 mRNA. Translation: BAE28343.1 .
    AK150466 mRNA. Translation: BAE29583.1 .
    AK168588 mRNA. Translation: BAE40457.1 .
    BC048693 mRNA. Translation: AAH48693.1 .
    CCDSi CCDS27379.1. [Q9QWZ1-1 ]
    CCDS70618.1. [Q9QWZ1-2 ]
    RefSeqi NP_001276376.1. NM_001289447.1. [Q9QWZ1-1 ]
    NP_001276377.1. NM_001289448.1. [Q9QWZ1-2 ]
    NP_035362.2. NM_011232.3. [Q9QWZ1-1 ]
    XP_006520102.1. XM_006520039.1. [Q9QWZ1-1 ]
    UniGenei Mm.38376.

    3D structure databases

    ProteinModelPortali Q9QWZ1.
    SMRi Q9QWZ1. Positions 15-275.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9QWZ1.

    Proteomic databases

    PRIDEi Q9QWZ1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022856 ; ENSMUSP00000022856 ; ENSMUSG00000022248 . [Q9QWZ1-1 ]
    ENSMUST00000100775 ; ENSMUSP00000098338 ; ENSMUSG00000022248 . [Q9QWZ1-2 ]
    GeneIDi 19355.
    KEGGi mmu:19355.
    UCSCi uc007vgh.1. mouse. [Q9QWZ1-1 ]

    Organism-specific databases

    CTDi 5810.
    MGIi MGI:1316678. Rad1.

    Phylogenomic databases

    eggNOGi NOG324428.
    GeneTreei ENSGT00500000044913.
    HOGENOMi HOG000008015.
    HOVERGENi HBG053058.
    InParanoidi Q9QWZ1.
    KOi K02830.
    OMAi CKVSVRT.
    OrthoDBi EOG7N0C4P.
    PhylomeDBi Q9QWZ1.
    TreeFami TF101211.

    Miscellaneous databases

    NextBioi 296395.
    PROi Q9QWZ1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9QWZ1.
    Bgeei Q9QWZ1.
    CleanExi MM_RAD1.
    Genevestigatori Q9QWZ1.

    Family and domain databases

    InterProi IPR003011. Cell_cycle_checkpoint_Rad1.
    IPR003021. Rad1_Rec1_Rad17.
    [Graphical view ]
    PANTHERi PTHR10870. PTHR10870. 1 hit.
    Pfami PF02144. Rad1. 1 hit.
    [Graphical view ]
    PRINTSi PR01245. RAD1REC1.
    PR01246. RAD1REPAIR.
    ProtoNeti Search...

    Publicationsi

    1. "Human and mouse homologs of Schizosaccharomyces pombe rad1(+) and Saccharomyces cerevisiae RAD17: linkage to checkpoint control and mammalian meiosis."
      Freire R., Murguia J.R., Tarsounas M., Lowndes N.F., Moens P.B., Jackson S.P.
      Genes Dev. 12:2560-2573(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    2. "A human and mouse homolog of the Schizosaccharomyces pombe rad1+ cell cycle checkpoint control gene."
      Bluyssen H.A.R., van Os R.I., Naus N.C., Jaspers I., Hoeijmakers J.H.J., de Klein A.
      Genomics 54:331-337(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    3. "cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster."
      Dean F.B., Lian L., O'Donnell M.
      Genomics 54:424-436(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    4. "A human homologue of the Schizosaccharomyces pombe rad1+ checkpoint gene encodes an exonuclease."
      Parker A.E., Van de Weyer I., Laus M.C., Oostveen I., Yon J., Verhasselt P., Luyten W.H.M.L.
      J. Biol. Chem. 273:18332-18339(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    5. "HRAD1 and MRad1 encode mammalian homologues of the fission yeast rad1+ cell cycle checkpoint control gene."
      Udell C.M., Lee S.K., Davey S.
      Nucleic Acids Res. 26:3971-3976(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    6. "Identification and cloning of Hrad1, a human homolog of the SchizosaCCharomyces pombe checkpoint protein."
      Hao L., Chang M., Liu J., Chen L.B.
      Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    7. "Mammalian REC1, encoding a 3'-5' exonuclease, is differentially expressed during meiosis."
      Shannon M.E., Naureckiene S., Stubbs L., Holloman W.K., Thelen M.P.
      Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    8. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Bone marrow, Fetal kidney and Heart.
    9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Limb.

    Entry informationi

    Entry nameiRAD1_MOUSE
    AccessioniPrimary (citable) accession number: Q9QWZ1
    Secondary accession number(s): O70452
    , O88391, Q3TGU1, Q3UG66, Q9QWZ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 7, 2006
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3