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Protein

Cell cycle checkpoint protein RAD1

Gene

Rad1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity (By similarity).By similarity

Catalytic activityi

Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-MMU-176187 Activation of ATR in response to replication stress
R-MMU-5685938 HDR through Single Strand Annealing (SSA)
R-MMU-5693607 Processing of DNA double-strand break ends
R-MMU-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-MMU-6804756 Regulation of TP53 Activity through Phosphorylation
R-MMU-69473 G2/M DNA damage checkpoint

Names & Taxonomyi

Protein namesi
Recommended name:
Cell cycle checkpoint protein RAD1 (EC:3.1.11.2)
Short name:
mRAD1
Alternative name(s):
DNA repair exonuclease rad1 homolog
Rad1-like DNA damage checkpoint protein
Gene namesi
Name:Rad1
Synonyms:Rec1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1316678 Rad1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002250061 – 280Cell cycle checkpoint protein RAD1Add BLAST280

Proteomic databases

PaxDbiQ9QWZ1
PeptideAtlasiQ9QWZ1
PRIDEiQ9QWZ1

PTM databases

PhosphoSitePlusiQ9QWZ1

Expressioni

Tissue specificityi

Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level). Expressed in brain, testis, kidney, heart, liver and lung.3 Publications

Gene expression databases

BgeeiENSMUSG00000022248
CleanExiMM_RAD1
ExpressionAtlasiQ9QWZ1 baseline and differential
GenevisibleiQ9QWZ1 MM

Interactioni

Subunit structurei

Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD1 interacts with POLB, FEN1, HUS1, HUS1B, RAD9A and RAD9B. Interacts with DNAJC7 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022856

Structurei

3D structure databases

ProteinModelPortaliQ9QWZ1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the rad1 family.Curated

Phylogenomic databases

eggNOGiKOG3194 Eukaryota
ENOG410YHQU LUCA
GeneTreeiENSGT00500000044913
HOGENOMiHOG000008015
HOVERGENiHBG053058
InParanoidiQ9QWZ1
KOiK02830
OMAiCKVSVRT
OrthoDBiEOG091G0HS8
PhylomeDBiQ9QWZ1
TreeFamiTF101211

Family and domain databases

InterProiView protein in InterPro
IPR003011 Cell_cycle_checkpoint_Rad1
IPR003021 Rad1_Rec1_Rad17
PANTHERiPTHR10870 PTHR10870, 1 hit
PfamiView protein in Pfam
PF02144 Rad1, 1 hit
PRINTSiPR01245 RAD1REC1
PR01246 RAD1REPAIR

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QWZ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPLLTQYNEE EYEQYCLVAS LDNVRNLSTV LKAIHFREHA TCFATKNGIK
60 70 80 90 100
VTVENAKCVQ ANAFIQADVF QEFVIQEESV TFRINLTILL DCLSIFGSSP
110 120 130 140 150
TPGTLTALRM CYQGYGHPLM LFLEEGGVVT VCKITTQEPE ETLDFDFCST
160 170 180 190 200
NVMNKIILQS EGLREAFSEL DMTGDVLQIT VSPDKPYFRL STFGNAGNSH
210 220 230 240 250
LDYPKDSDLV EAFHCDKTQV NRYKLSLLKP STKALALSCK VSIRTDNRGF
260 270 280
LSLQYMIRNE DGQICFVEYY CCPDEEVPES
Length:280
Mass (Da):31,610
Last modified:May 1, 2000 - v1
Checksum:iF9D6ED292C5D4361
GO
Isoform 2 (identifier: Q9QWZ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-280: YKLSLLKPST...CCPDEEVPES → ISDVKYSCKT...QWVNVVPVQA

Show »
Length:272
Mass (Da):30,565
Checksum:i36207A21BA05BCF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28S → F (PubMed:9878245).Curated1
Sequence conflicti28S → F (PubMed:9660799).Curated1
Sequence conflicti28S → F (Ref. 6) Curated1
Sequence conflicti28S → F (Ref. 7) Curated1
Sequence conflicti71Q → E in BAE28343 (PubMed:16141072).Curated1
Sequence conflicti118P → T in BAE40457 (PubMed:16141072).Curated1
Sequence conflicti198N → S in AAC95465 (PubMed:9828137).Curated1
Sequence conflicti216D → N in AAC27248 (PubMed:9705507).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017337223 – 280YKLSL…EVPES → ISDVKYSCKTWLQLPEGVGY DPLSGLEWTLSERDGEQEVA QWVNVVPVQA in isoform 2. 1 PublicationAdd BLAST58

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF074718 mRNA Translation: AAC98094.1
AF073523 mRNA Translation: AAC95465.1
AF076842 mRNA Translation: AAC95524.1
AJ004976 mRNA Translation: CAA06250.1
AF038841 mRNA Translation: AAC27248.1
AF058394 mRNA Translation: AAC14139.1
AF084514 mRNA Translation: AAC35551.1
AK146533 mRNA Translation: BAE27240.1
AK148098 mRNA Translation: BAE28343.1
AK150466 mRNA Translation: BAE29583.1
AK168588 mRNA Translation: BAE40457.1
BC048693 mRNA Translation: AAH48693.1
CCDSiCCDS27379.1 [Q9QWZ1-1]
CCDS70618.1 [Q9QWZ1-2]
RefSeqiNP_001276376.1, NM_001289447.1 [Q9QWZ1-1]
NP_001276377.1, NM_001289448.1 [Q9QWZ1-2]
NP_035362.2, NM_011232.3 [Q9QWZ1-1]
UniGeneiMm.38376

Genome annotation databases

EnsembliENSMUST00000022856; ENSMUSP00000022856; ENSMUSG00000022248 [Q9QWZ1-1]
ENSMUST00000100775; ENSMUSP00000098338; ENSMUSG00000022248 [Q9QWZ1-2]
GeneIDi19355
KEGGimmu:19355
UCSCiuc007vgh.2 mouse [Q9QWZ1-1]
uc033gtc.1 mouse [Q9QWZ1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRAD1_MOUSE
AccessioniPrimary (citable) accession number: Q9QWZ1
Secondary accession number(s): O70452
, O88391, Q3TGU1, Q3UG66, Q9QWZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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