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Protein

Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Gene

Asap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types. Plays a role in ciliogenesis (By similarity). Posseses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2.By similarity

Enzyme regulationi

Activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri469 – 492C4-typePROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: MGI
  • GTPase activator activity Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • phosphatidylinositol-3,4,5-trisphosphate binding Source: FlyBase
  • phosphatidylinositol-4,5-bisphosphate binding Source: FlyBase
  • phosphatidylserine binding Source: MGI

GO - Biological processi

  • cilium morphogenesis Source: UniProtKB
  • negative regulation of dendritic spine development Source: MGI
  • positive regulation of membrane tubulation Source: MGI
  • positive regulation of podosome assembly Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5620916. VxPx cargo-targeting to cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
Alternative name(s):
130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein
ADP-ribosylation factor-directed GTPase-activating protein 1
Short name:
ARF GTPase-activating protein 1
Development and differentiation-enhancing factor 1
Short name:
DEF-1
Short name:
Differentiation-enhancing factor 1
PIP2-dependent ARF1 GAP
Gene namesi
Name:Asap1
Synonyms:Ddef1, Kiaa1249, Shag1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1342335. Asap1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: MGI
  • cell projection membrane Source: MGI
  • cytosol Source: Reactome
  • dendritic spine Source: MGI
  • podosome Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi811R → A: Significant reduction in binding to SRC and CRK and loss of phosphorylation. Loss of binding and phosphorylation; when associated with A-910 and A-913. 1 Publication1
Mutagenesisi910P → A: Significant reduction in binding to SRC and CRK and decrease in phosphorylation; when associated with A-913. Loss of binding and phosphorylation; when associated with A-811 and A-913. 1 Publication1
Mutagenesisi913P → A: Significant reduction in binding to SRC and CRK and decrease in phosphorylation; when associated with A-910. Loss of binding and phosphorylation; when associated with A-811 and A-910. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741971 – 1147Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1Add BLAST1147

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei308Phosphotyrosine; by FAK21 Publication1
Modified residuei732PhosphoserineCombined sources1
Modified residuei741PhosphoserineBy similarity1
Modified residuei854PhosphoserineBy similarity1
Modified residuei858PhosphoserineCombined sources1
Modified residuei1026PhosphoserineBy similarity1
Modified residuei1045PhosphoserineCombined sources1
Modified residuei1059PhosphoserineCombined sources1
Modified residuei1066PhosphothreonineCombined sources1
Modified residuei1146PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine residues by SRC.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QWY8.
PRIDEiQ9QWY8.

PTM databases

iPTMnetiQ9QWY8.
PhosphoSitePlusiQ9QWY8.

Miscellaneous databases

PMAP-CutDBQ9QWY8.

Expressioni

Tissue specificityi

Expressed in all tissues examined but a most abundant expression was found in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals.1 Publication

Gene expression databases

BgeeiENSMUSG00000022377.
ExpressionAtlasiQ9QWY8. baseline and differential.
GenevisibleiQ9QWY8. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with SRC and CRK. Interacts with RAB11FIP3. Interacts with PTK2B/PYK2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SRCP005233EBI-698517,EBI-848039From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199076. 20 interactors.
IntActiQ9QWY8. 8 interactors.
MINTiMINT-266354.
STRINGi10090.ENSMUSP00000134825.

Structurei

Secondary structure

11147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi335 – 337Combined sources3
Beta strandi342 – 349Combined sources8
Beta strandi351 – 354Combined sources4
Beta strandi357 – 365Combined sources9
Beta strandi368 – 371Combined sources4
Beta strandi374 – 378Combined sources5
Beta strandi381 – 384Combined sources4
Helixi385 – 387Combined sources3
Beta strandi389 – 392Combined sources4
Beta strandi394 – 404Combined sources11
Beta strandi407 – 412Combined sources6
Helixi416 – 436Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5C6RX-ray1.80A/B325-451[»]
5C79X-ray1.60A/B325-451[»]
ProteinModelPortaliQ9QWY8.
SMRiQ9QWY8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini339 – 431PHPROSITE-ProRule annotationAdd BLAST93
Domaini454 – 577Arf-GAPPROSITE-ProRule annotationAdd BLAST124
Repeati615 – 647ANK 1Add BLAST33
Repeati651 – 680ANK 2Add BLAST30
Domaini1085 – 1147SH3PROSITE-ProRule annotationAdd BLAST63

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi798 – 1011Pro-richAdd BLAST214

Domaini

The PH domain most probably contributes to the phosphoinositide-dependent regulation of ADP ribosylation factors.

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 Arf-GAP domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri469 – 492C4-typePROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

ANK repeat, Repeat, SH3 domain, Zinc-finger

Phylogenomic databases

eggNOGiKOG0521. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000230570.
HOVERGENiHBG051327.
InParanoidiQ9QWY8.
KOiK12488.
OMAiTEWGNTA.
TreeFamiTF325156.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QWY8-1) [UniParc]FASTAAdd to basket
Also known as: SHAG1a, ASAP1a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSSASRLSS FSSRDSLWNR MPDQISVSEF IAETTEDYNS PTTSSFTTRL
60 70 80 90 100
HNCRNTVTLL EEALDQDRTA LQKVKKSVKA IYNSGQDHVQ NEENYAQVLD
110 120 130 140 150
KFGSNFLSRD NPDLGTAFVK FSTLTKELST LLKNLLQGLS HNVIFTLDSL
160 170 180 190 200
LKGDLKGVKG DLKKPFDKAW KDYETKFTKI EKEKREHAKQ HGMIRTEITG
210 220 230 240 250
AEIAEEMEKE RRLFQLQMCE YLIKVNEIKT KKGVDLLQNL IKYYHAQCNF
260 270 280 290 300
FQDGLKTADK LKQYIEKLAA DLYNIKQTQD EEKKQLTALR DLIKSSLQLD
310 320 330 340 350
PKEVGGLYVA SRANSSRRDS QSRQGGYSMH QLQGNKEYGS EKKGFLLKKS
360 370 380 390 400
DGIRKVWQRR KCAVKNGILT ISHATSNRQP AKLNLLTCQV KPNAEDKKSF
410 420 430 440 450
DLISHNRTYH FQAEDEQDYI AWISVLTNSK EEALTMAFRG EQSTGENSLE
460 470 480 490 500
DLTKAIIEDV QRLPGNDICC DCGSSEPTWL STNLGILTCI ECSGIHREMG
510 520 530 540 550
VHISRIQSLE LDKLGTSELL LAKNVGNNSF NDIMEANLPS PSPKPTPSSD
560 570 580 590 600
MTVRKEYITA KYVDHRFSRK TCASSSAKLN ELLEAIKSRD LLALIQVYAE
610 620 630 640 650
GVELMEPLLE PGQELGETAL HLAVRTADQT SLHLVDFLVQ NCGNLDKQTS
660 670 680 690 700
VGNTVLHYCS MYGKPECLKL LLRSKPTVDI VNQNGETALD IAKRLKATQC
710 720 730 740 750
EDLLSQAKSG KFNPHVHVEY EWNLRQDEMD ESDDDLDDKP SPIKKERSPR
760 770 780 790 800
PQSFCHSSSI SPQDKLALPG FSTPRDKQRL SYGAFTNQIF ASTSTDLPTS
810 820 830 840 850
PTSEAPPLPP RNAGKGPTGP PSTLPLGTQT SSGSSTLSKK RPPPPPPGHK
860 870 880 890 900
RTLSDPPSPL PHGPPNKGAI PWGNDVGPLS SSKTANKFEG LSQQASTSSA
910 920 930 940 950
KTALGPRVLP KLPQKVALRK TETSHHLSLD RTNIPPETFQ KSSQLTELPQ
960 970 980 990 1000
KPPLGELPPK PVELAPKPQV GELPPKPGEL PPKPQLGDLP PKPQLSDLPP
1010 1020 1030 1040 1050
KPQMKDLPPK PQLGDLLAKS QAGDVSAKVQ PPSEVTQRSH TGDLSPNVQS
1060 1070 1080 1090 1100
RDAIQKQASE DSNDLTPTLP ETPVPLPRKI NTGKNKVRRV KTIYDCQADN
1110 1120 1130 1140
DDELTFIEGE VIIVTGEEDQ EWWIGHIEGQ PERKGVFPVS FVHILSD
Length:1,147
Mass (Da):127,421
Last modified:October 3, 2012 - v2
Checksum:i12950D1C4F49A909
GO
Isoform 2 (identifier: Q9QWY8-2) [UniParc]FASTAAdd to basket
Also known as: SHAG1b, ASAP1b

The sequence of this isoform differs from the canonical sequence as follows:
     816-872: Missing.

Show »
Length:1,090
Mass (Da):121,675
Checksum:i5C1F1D950B7B252A
GO
Isoform 3 (identifier: Q9QWY8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-315: Missing.

Show »
Length:1,135
Mass (Da):126,246
Checksum:i636A1D0E72FB8893
GO
Isoform 4 (identifier: Q9QWY8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     304-318: Missing.

Show »
Length:1,132
Mass (Da):125,846
Checksum:i32751FB034E7CD51
GO

Sequence cautioni

The sequence AAH02201 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAH48818 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti654T → S in AAB82338 (PubMed:9126384).Curated1
Sequence conflicti879L → S in AAC98349 (PubMed:9819391).Curated1
Sequence conflicti879L → S in AAC98350 (PubMed:9819391).Curated1
Sequence conflicti1051R → I in AAB82338 (PubMed:9126384).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008367304 – 318Missing in isoform 4. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_008366304 – 315Missing in isoform 3. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_008368816 – 872Missing in isoform 2. 1 PublicationAdd BLAST57

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF075461 mRNA. Translation: AAC98349.1.
AF075462 mRNA. Translation: AAC98350.1.
AC098728 Genomic DNA. No translation available.
AC131710 Genomic DNA. No translation available.
AC156573 Genomic DNA. No translation available.
BC002201 mRNA. Translation: AAH02201.1. Different initiation.
BC048818 mRNA. Translation: AAH48818.1. Different initiation.
BC094581 mRNA. Translation: AAH94581.1.
AK122477 mRNA. Translation: BAC65759.1.
U92478 mRNA. Translation: AAB82338.1.
CCDSiCCDS56986.1. [Q9QWY8-1]
CCDS70634.1. [Q9QWY8-2]
PIRiT42627.
RefSeqiNP_001263390.1. NM_001276461.1.
NP_001263391.1. NM_001276462.1.
NP_001263396.1. NM_001276467.1. [Q9QWY8-2]
NP_034156.2. NM_010026.3. [Q9QWY8-1]
UniGeneiMm.277236.
Mm.491089.

Genome annotation databases

EnsembliENSMUST00000110115; ENSMUSP00000105742; ENSMUSG00000022377. [Q9QWY8-4]
ENSMUST00000175793; ENSMUSP00000135718; ENSMUSG00000022377. [Q9QWY8-3]
ENSMUST00000176384; ENSMUSP00000135190; ENSMUSG00000022377. [Q9QWY8-2]
ENSMUST00000177374; ENSMUSP00000134825; ENSMUSG00000022377. [Q9QWY8-1]
GeneIDi13196.
KEGGimmu:13196.
UCSCiuc007vzh.2. mouse. [Q9QWY8-3]
uc007vzk.2. mouse. [Q9QWY8-1]
uc007vzl.2. mouse. [Q9QWY8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF075461 mRNA. Translation: AAC98349.1.
AF075462 mRNA. Translation: AAC98350.1.
AC098728 Genomic DNA. No translation available.
AC131710 Genomic DNA. No translation available.
AC156573 Genomic DNA. No translation available.
BC002201 mRNA. Translation: AAH02201.1. Different initiation.
BC048818 mRNA. Translation: AAH48818.1. Different initiation.
BC094581 mRNA. Translation: AAH94581.1.
AK122477 mRNA. Translation: BAC65759.1.
U92478 mRNA. Translation: AAB82338.1.
CCDSiCCDS56986.1. [Q9QWY8-1]
CCDS70634.1. [Q9QWY8-2]
PIRiT42627.
RefSeqiNP_001263390.1. NM_001276461.1.
NP_001263391.1. NM_001276462.1.
NP_001263396.1. NM_001276467.1. [Q9QWY8-2]
NP_034156.2. NM_010026.3. [Q9QWY8-1]
UniGeneiMm.277236.
Mm.491089.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5C6RX-ray1.80A/B325-451[»]
5C79X-ray1.60A/B325-451[»]
ProteinModelPortaliQ9QWY8.
SMRiQ9QWY8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199076. 20 interactors.
IntActiQ9QWY8. 8 interactors.
MINTiMINT-266354.
STRINGi10090.ENSMUSP00000134825.

PTM databases

iPTMnetiQ9QWY8.
PhosphoSitePlusiQ9QWY8.

Proteomic databases

PaxDbiQ9QWY8.
PRIDEiQ9QWY8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000110115; ENSMUSP00000105742; ENSMUSG00000022377. [Q9QWY8-4]
ENSMUST00000175793; ENSMUSP00000135718; ENSMUSG00000022377. [Q9QWY8-3]
ENSMUST00000176384; ENSMUSP00000135190; ENSMUSG00000022377. [Q9QWY8-2]
ENSMUST00000177374; ENSMUSP00000134825; ENSMUSG00000022377. [Q9QWY8-1]
GeneIDi13196.
KEGGimmu:13196.
UCSCiuc007vzh.2. mouse. [Q9QWY8-3]
uc007vzk.2. mouse. [Q9QWY8-1]
uc007vzl.2. mouse. [Q9QWY8-2]

Organism-specific databases

CTDi50807.
MGIiMGI:1342335. Asap1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0521. Eukaryota.
COG5347. LUCA.
GeneTreeiENSGT00760000118874.
HOGENOMiHOG000230570.
HOVERGENiHBG051327.
InParanoidiQ9QWY8.
KOiK12488.
OMAiTEWGNTA.
TreeFamiTF325156.

Enzyme and pathway databases

ReactomeiR-MMU-5620916. VxPx cargo-targeting to cilium.

Miscellaneous databases

ChiTaRSiAsap1. mouse.
PMAP-CutDBQ9QWY8.
PROiQ9QWY8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022377.
ExpressionAtlasiQ9QWY8. baseline and differential.
GenevisibleiQ9QWY8. MM.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
1.25.40.20. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001164. ArfGAP.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF01412. ArfGap. 1 hit.
PF00169. PH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00405. REVINTRACTNG.
SMARTiSM00248. ANK. 2 hits.
SM00105. ArfGap. 1 hit.
SM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50115. ARFGAP. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASAP1_MOUSE
AccessioniPrimary (citable) accession number: Q9QWY8
Secondary accession number(s): O08612
, Q505F0, Q80TG8, Q80UV6, Q99LV8, Q9Z2B6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: October 3, 2012
Last modified: November 2, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.