Q9QWW1 (HOME2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homer protein homolog 2
Short name=Homer-2

Alternative name(s):
Cupidin
VASP/Ena-related gene up-regulated during seizure and LTP 2
Short name=Vesl-2

Gene names

Name:	Homer2
Synonyms:	Vesl2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoforms can be differently regulated and may play an important role in maintaining the plasticity at glutamatergic synapses.
Subunit structure	Isoform 1 and isoform 2 encode coiled-coil structures that mediate homo- and heteromultimerization.
Subcellular location	Cytoplasm. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell junction › synapse. Note: Postsynaptic density of neuronal cells. The stabilization and clustering of the metabotropic glutamate receptors appears to be mediated by isoform 1 and isoform 2 at the cell surface.
Tissue specificity	Expressed in olfactory bulb, hippocampus, thalmus and heart. Ref.4
Sequence similarities	Belongs to the Homer family. Contains 1 WH1 domain.

Ontologies

Keywords
Cellular component	Cell junction Cell membrane Cytoplasm Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G-protein coupled glutamate receptor signaling pathway Traceable author statement. Source: MGI
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from direct assay. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic density Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GKAP/Homer scaffold activity Inferred from direct assay. Source: MGI actin binding Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9QWW1-1)

Also known as: 2b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9QWW1-2)

Also known as: 2a;

The sequence of this isoform differs from the canonical sequence as follows:
     130-140: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 354

354

Homer protein homolog 2

PRO_0000191009

Regions

Domain

1 – 110

110

WH1

Coiled coil

160 – 329

170

Potential

Natural variations

Alternative sequence

130 – 140

11

Missing in isoform 2.

VSP_009071

Experimental info

Sequence conflict

191 – 192

2

RE → VP in CAA09909. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

354

Helix Strand Turn

Details...

Beta strand

6 – 15

10

Beta strand

32 – 39

8

Turn

40 – 43

4

Beta strand

44 – 51

8

Beta strand

54 – 61

8

Beta strand

67 – 79

13

Turn

80 – 83

4

Beta strand

84 – 89

6

Helix

93 – 110

18

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (2b) [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 18AF2F22EA8E1D06	FASTA	354	40,570
	10 20 30 40 50 60 MGEQPIFTTR AHVFQIDPST KKNWVPASKQ AVTVSYFYDV TRNSYRIISV DGAKVIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVFGLGF SSELQLTKFA EKFQEVREAA RLARDKSQEK 130 140 150 160 170 180 TETSSNHSQE SGCETPSSTQ ASSVNGTDDE KASHASPADT HLKSENDKLK IALTQSAANV 190 200 210 220 230 240 KKWEMELQTL RESNARLTTA LQESAASVEQ WKRQFSICRD ENDRLRSKIE ELEEQCSEIN 250 260 270 280 290 300 REKEKNTQLK RRIEELESEV RDKEMELKDL RKQSEIIPQL MSECEYVSEK LEAAERDNQN 310 320 330 340 350 LEDKVRSLKT DIEESKYRQR HLKGELKSFL EVLDGKIDDL HDFRRGLSKL GTDN
	Isoform 2 (2a) [UniParc]. Checksum: 417769609B7D655E	FASTA	343	39,463
	10 20 30 40 50 60 MGEQPIFTTR AHVFQIDPST KKNWVPASKQ AVTVSYFYDV TRNSYRIISV DGAKVIINST 70 80 90 100 110 120 ITPNMTFTKT SQKFGQWADS RANTVFGLGF SSELQLTKFA EKFQEVREAA RLARDKSQEK 130 140 150 160 170 180 TETSSNHSQA SSVNGTDDEK ASHASPADTH LKSENDKLKI ALTQSAANVK KWEMELQTLR 190 200 210 220 230 240 ESNARLTTAL QESAASVEQW KRQFSICRDE NDRLRSKIEE LEEQCSEINR EKEKNTQLKR 250 260 270 280 290 300 RIEELESEVR DKEMELKDLR KQSEIIPQLM SECEYVSEKL EAAERDNQNL EDKVRSLKTD 310 320 330 340 IEESKYRQRH LKGELKSFLE VLDGKIDDLH DFRRGLSKLG TDN

References

[1]	"Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins." Xiao B., Tu J.C., Petralia R.S., Yuan J.P., Doan A., Breder C.D., Ruggiero A., Lanahan A.A., Wenthold R.J., Worley P.F. Neuron 21:707-716(1998) [PubMed: 9808458] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Forebrain.
[2]	"Cupidin, a gene transiently expressed in developing cerebellar granule cells." Shiraishi Y., Mizutani A., Furuichi T. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Cerebellum.
[3]	Keen J., Inglehearn C. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-192.
[4]	"Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors." Tu J.C., Xiao B., Yuan J.P., Lanahan A.A., Leoffert K., Li M., Linden D.J., Worley P.F. Neuron 21:717-726(1998) [PubMed: 9808459] [Abstract] Cited for: INTERACTION WITH GRM1; GRM5; DYN3 AND ITPR1, TISSUE SPECIFICITY.
[5]	"The N-terminal domain of Homer/Vesl is a new class II EVH1 domain." Barzik M., Carl U.D., Schubert W.-D., Frank R., Wehland J., Heinz D.W. J. Mol. Biol. 309:155-169(2001) [PubMed: 11491285] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.24 ANGSTROMS) OF 2-111.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF093259 mRNA. Translation: AAC71023.1.
AF093260 mRNA. Translation: AAC71024.1.
AB017136 mRNA. Translation: BAA37088.1.
AJ012076 mRNA. Translation: CAA09909.1.

IPI

IPI00134508.
IPI00395070.

RefSeq

NP_001157558.1. NM_001164086.1.
NP_001157559.1. NM_001164087.1.
NP_036113.1. NM_011983.2.

UniGene

Mm.228.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I7A	X-ray	2.24	A/B/C/D	1-111	[»]

ProteinModelPortal

Q9QWW1.

SMR

Q9QWW1. Positions 3-111, 287-352.

ModBase

Search...

Protein-protein interaction databases

STRING

Q9QWW1.

Proteomic databases

PRIDE

Q9QWW1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000026922; ENSMUSP00000026922; ENSMUSG00000025813.

GeneID

26557.

KEGG

mmu:26557.

UCSC

uc009ich.2. mouse.
uc009ici.2. mouse.

Organism-specific databases

CTD

9455.

MGI

MGI:1347354. Homer2.

Phylogenomic databases

eggNOG

roNOG12514.

GeneTree

ENSGT00390000017850.

HOGENOM

HBG443677.

HOVERGEN

HBG051918.

InParanoid

Q9QWW1.

OMA

DLHDFRR.

OrthoDB

EOG4DFPNT.

PhylomeDB

Q9QWW1.

Gene expression databases

ArrayExpress

Q9QWW1.

Bgee

Q9QWW1.

CleanEx

MM_HOMER2.

Genevestigator

Q9QWW1.

GermOnline

ENSMUSG00000025813. Mus musculus.

Family and domain databases

InterPro

IPR000697. EVH1.
IPR011993. PH_type.
[Graphical view]

Gene3D

G3DSA:2.30.29.30. PH_type. 1 hit.

KO

K15010.

Pfam

PF00568. WH1. 1 hit.
[Graphical view]

SMART

SM00461. WH1. 1 hit.
[Graphical view]

PROSITE

PS50229. WH1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

304611.

SOURCE

Search...

Entry information

Entry name

HOME2_MOUSE

Accession

Primary (citable) accession number: Q9QWW1
Secondary accession number(s): O89025, Q9Z0E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program