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Protein

Cyclin-T1

Gene

Ccnt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).

GO - Molecular functioni

  • 7SK snRNA binding Source: UniProtKB
  • chromatin binding Source: MGI
  • cyclin-dependent protein serine/threonine kinase regulator activity Source: GO_Central
  • DNA binding Source: MGI
  • protein kinase binding Source: MGI
  • RNA polymerase binding Source: MGI
  • snRNA binding Source: MGI
  • transcription factor binding Source: MGI
  • transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionCyclin
Biological processCell cycle, Cell division, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-112387. Elongation arrest and recovery.
R-MMU-2173796. SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-MMU-6807505. RNA polymerase II transcribes snRNA genes.
R-MMU-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-T1
Short name:
CycT1
Short name:
Cyclin-T
Gene namesi
Name:Ccnt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1328363. Ccnt1.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi256R → W: No effect. 1 Publication1
Mutagenesisi261Y → C: Binding to HIV-1 Tat similar to human CCNT1. 2 Publications1
Mutagenesisi262Q → E: No effect. 1 Publication1
Mutagenesisi265M → K: No effect. 1 Publication1
Mutagenesisi277N → K: No effect. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000804931 – 724Cyclin-T1Add BLAST724

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117PhosphoserineBy similarity1
Cross-linki342Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei388PhosphoserineBy similarity1
Modified residuei390N6-acetyllysineBy similarity1
Cross-linki415Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki480Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei494PhosphoserineBy similarity1
Modified residuei498PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9QWV9.
MaxQBiQ9QWV9.
PaxDbiQ9QWV9.
PeptideAtlasiQ9QWV9.
PRIDEiQ9QWV9.

PTM databases

iPTMnetiQ9QWV9.
PhosphoSitePlusiQ9QWV9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000011960.
CleanExiMM_CCNT1.
ExpressionAtlasiQ9QWV9. baseline and differential.
GenevisibleiQ9QWV9. MM.

Interactioni

Subunit structurei

Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with BRD4, probably to target chromatin binding. Interacts with MDFIC. Interacts with HSF1. Interacts with HTATSF1. Interacts with AFF4.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein kinase binding Source: MGI
  • RNA polymerase binding Source: MGI
  • transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi198559. 1 interactor.
IntActiQ9QWV9. 7 interactors.
MINTiMINT-4090105.
STRINGi10090.ENSMUSP00000012104.

Structurei

3D structure databases

ProteinModelPortaliQ9QWV9.
SMRiQ9QWV9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili384 – 425Sequence analysisAdd BLAST42

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi253 – 270Nuclear localization signalSequence analysisAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi319 – 322Poly-Ser4
Compositional biasi505 – 529His-richAdd BLAST25
Compositional biasi565 – 569Poly-Ser5
Compositional biasi706 – 723Pro-richAdd BLAST18

Sequence similaritiesi

Belongs to the cyclin family. Cyclin C subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0834. Eukaryota.
COG5333. LUCA.
GeneTreeiENSGT00760000119191.
HOGENOMiHOG000013208.
HOVERGENiHBG050843.
InParanoidiQ9QWV9.
KOiK15188.
OMAiTMAQLPG.
OrthoDBiEOG091G03Z2.
PhylomeDBiQ9QWV9.
TreeFamiTF101014.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
InterProiView protein in InterPro
IPR028863. CCNT1.
IPR013763. Cyclin-like.
IPR006671. Cyclin_N.
PANTHERiPTHR10026:SF84. PTHR10026:SF84. 1 hit.
PfamiView protein in Pfam
PF00134. Cyclin_N. 1 hit.
SMARTiView protein in SMART
SM00385. CYCLIN. 1 hit.
SUPFAMiSSF47954. SSF47954. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9QWV9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGERKNNNK RWYFTREQLE NSPSRRFGVD SDKELSYRQQ AANLLQDMGQ
60 70 80 90 100
RLNVSQLTIN TAIVYMHRFY MIQSFTQFHR YSMAPAALFL AAKVEEQPKK
110 120 130 140 150
LEHVIKVAHT CLHPQESLPD TRSEAYLQQV QDLVILESII LQTLGFELTI
160 170 180 190 200
DHPHTHVVKC TQLVRASKDL AQTSYFMATN SLHLTTFSLQ YTPPVVACVC
210 220 230 240 250
IHLACKWSNW EIPVSTDGKH WWEYVDATVT LELLDELTHE FLQILEKTPS
260 270 280 290 300
RLKRIRNWRA YQAAMKTKPD DRGADENTSE QTILNMISQT SSDTTIAGLM
310 320 330 340 350
SMSTASTSAV PSLPSSEESS SSLTSVDMLQ GERWLSSQPP FKLEAAQGHR
360 370 380 390 400
TSESLALIGV DHSLQQDGSS AFGSQKQASK SVPSAKVSLK EYRAKHAEEL
410 420 430 440 450
AAQKRQLENM EANVKSQYAY AAQNLLSHDS HSSVILKMPI ESSENPERPF
460 470 480 490 500
LDKADKSALK MRLPVASGDK AVSSKPEEIK MRIKVHSAGD KHNSIEDSVT
510 520 530 540 550
KSREHKEKQR THPSNHHHHH NHHSHRHSHL QLPAGPVSKR PSDPKHSSQT
560 570 580 590 600
STLAHKTYSL SSTLSSSSST RKRGPPEETG AAVFDHPAKI AKSTKSSLNF
610 620 630 640 650
PFPPLPTMTQ LPGHSSDTSG LPFSQPSCKT RVPHMKLDKG PPGANGHNAT
660 670 680 690 700
QSIDYQDTVN MLHSLLSAQG VQPTQAPAFE FVHSYGEYMN PRAGAISSRS
710 720
GTTDKPRPPP LPSEPPPPLP PLPK
Length:724
Mass (Da):80,598
Last modified:October 3, 2012 - v3
Checksum:i6D090F5CA7C3A79C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48M → V in AAD19654 (PubMed:10329126).Curated1
Sequence conflicti567S → F in AAD17205 (PubMed:9990016).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095640 mRNA. Translation: AAD13656.1.
AF109179 mRNA. Translation: AAD19654.1.
AF087662 mRNA. Translation: AAD17798.1.
AF113951 mRNA. Translation: AAD17205.1.
AK133168 mRNA. Translation: BAE21540.1.
AC138221 Genomic DNA. No translation available.
BC131685 mRNA. Translation: AAI31686.1.
CCDSiCCDS27797.1.
RefSeqiNP_033963.1. NM_009833.1.
UniGeneiMm.29941.
Mm.86538.

Genome annotation databases

EnsembliENSMUST00000012104; ENSMUSP00000012104; ENSMUSG00000011960.
ENSMUST00000169707; ENSMUSP00000126874; ENSMUSG00000011960.
GeneIDi12455.
KEGGimmu:12455.
UCSCiuc007xmv.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCCNT1_MOUSE
AccessioniPrimary (citable) accession number: Q9QWV9
Secondary accession number(s): Q3V0G4, Q9Z0U7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 3, 2012
Last modified: August 30, 2017
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families