Q9QWT9 (KIFC1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-like protein KIFC1

Gene names

Name:	Kifc1
Synonyms:	Kifc4, Kifc5a, Kifc5b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles. Ref.3 Ref.9
Subunit structure	Binds NUBP1 and NUBP2. Interacts with PPP1R42. Ref.3 Ref.10
Subcellular location	Nucleus. Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › spindle. Early endosome. Note: Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis. Ref.3 Ref.9
Tissue specificity	Highly expressed in E14 embryos, spleen and NIH3T3 cells. Also expressed in testis, brain, lung, kidney and cultured astrocytes. Very low levels in skeletal muscle and heart. Ref.1 Ref.3
Developmental stage	Highly expressed in hippocampus of E13 embryos declining to low levels by E18 and to undetectable levels in juvenile and adult hippocampus. Ref.3
Disruption phenotype	Centrosome amplification as well as multipolar spindles. Cells overexpressing Kifc1 show a single microtubule aster and growth arrest in prometaphase. Ref.3
Miscellaneous	Purified early endocytic vesicles bind minus end-directed Kifc1 as well as plus end-directed Kif5b. Addition of anti-Kifc1 antibodies leads to a decrease in minus end-directed vesicle motility in vitro. Ref.9
Sequence similarities	Belongs to the kinesin-like protein family. NCD subfamily. Contains 1 kinesin-motor domain.
Sequence caution	The sequence BAA19676.1 differs from that shown. Reason: Frameshift at positions 223 and 244.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Endosome Microtubule Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule-based movement Inferred from electronic annotation. Source: InterPro mitosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	early endosome Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule associated complex Inferred from electronic annotation. Source: InterPro microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell spindle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.2 (identifier: Q9QWT9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.3 (identifier: Q9QWT9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.

Isoform 3 Ref.1 (identifier: Q9QWT9-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: Missing.
     203-222: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 674	674	Kinesin-like protein KIFC1		PRO_0000302089
Regions
	Domain	308 – 595	288	Kinesin-motor
	Nucleotide binding	411 – 418	8	ATP Potential
	Coiled coil	146 – 315	170	Potential
Amino acid modifications
	Modified residue	33	1	Phosphoserine By similarity
	Modified residue	35	1	Phosphoserine Ref.11
Natural variations
	Alternative sequence	1 – 43	43	Missing in isoform 2 and isoform 3. Ref.1 Ref.3		VSP_052526
	Alternative sequence	203 – 222	20	Missing in isoform 3. Ref.1		VSP_052527
Experimental info
	Sequence conflict	101	1	I → V in BAA19676. Ref.1
	Sequence conflict	101	1	I → V in AAF34646. Ref.2
	Sequence conflict	101	1	I → V in CAJ19646. Ref.3
	Sequence conflict	101	1	I → V in AAH03753. Ref.5
	Sequence conflict	101	1	I → V in AAH57162. Ref.5
	Sequence conflict	101	1	I → V in AAI00329. Ref.5
	Sequence conflict	111	1	V → A in BAA19676. Ref.1
	Sequence conflict	111	1	V → A in AAF34646. Ref.2
	Sequence conflict	111	1	V → A in CAJ19646. Ref.3
	Sequence conflict	111	1	V → A in AAH03753. Ref.5
	Sequence conflict	111	1	V → A in AAH57162. Ref.5
	Sequence conflict	111	1	V → A in AAI00329. Ref.5
	Sequence conflict	160	1	T → M in BAA19676. Ref.1
	Sequence conflict	160	1	T → M in AAF34646. Ref.2
	Sequence conflict	160	1	T → M in CAJ19646. Ref.3
	Sequence conflict	253	1	E → K in BAA19676. Ref.1
	Sequence conflict	253	1	E → K in AAF34646. Ref.2
	Sequence conflict	253	1	E → K in CAJ19646. Ref.3
	Sequence conflict	253	1	E → K in AAH03753. Ref.5
	Sequence conflict	271	1	V → L in AAH57162. Ref.5
	Sequence conflict	315	1	F → L in CAJ19646. Ref.3
	Sequence conflict	323	1	E → A in BAA19676. Ref.1
	Sequence conflict	323	1	E → A in AAF34646. Ref.2
	Sequence conflict	323	1	E → A in CAJ19646. Ref.3
	Sequence conflict	323	1	E → A in AAH03753. Ref.5
	Sequence conflict	323	1	E → A in AAI00329. Ref.5
	Sequence conflict	345	1	P → L in AAI00329. Ref.5
	Sequence conflict	432	1	E → A in BAC38230. Ref.7
	Sequence conflict	478	1	P → L in BAA19676. Ref.1
	Sequence conflict	503	1	A → V in BAA19676. Ref.1
	Sequence conflict	566	1	D → G in AAF34646. Ref.2
	Sequence conflict	578	1	H → P in BAC38230. Ref.7
	Sequence conflict	619	1	S → R in BAA19676. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: A99B6FB6F9BC1B03	FASTA	674	74,153
	10 20 30 40 50 60 MDVQAQRPPL LEVKRNVELK AALVKSSSRV PLSASRLKRG PDQMEDALEP AKKRTRVMGA 70 80 90 100 110 120 VTKVDTSRPR GPLLSTVSQT QGHTAAQKGP KKTGPRGCSA IGTVLRSQKP VPAAPAQKPG 130 140 150 160 170 180 TSTAPVVVGK RAGKRPAWDL KGQLCDLNEE LKRYREKTQT LELENRGLRE QLREVQEQAT 190 200 210 220 230 240 TLGTERNTLE GELASVRSRA EQDQQRLETL SARVLELEEC LGTRERLLQE LQGERLQLQE 250 260 270 280 290 300 ERSTLSTQLE EQERRFQATE AALSSSQEEV VCLRQKTEAQ VTLLAEQGDR LYGLEMERRR 310 320 330 340 350 360 LHNQLQELKG NIRVFCRVRP VLEGESTPSP GFLVFPPGPA GPSDPPTGLS LSRSDDRRST 370 380 390 400 410 420 LTGAPAPTVR HDFSFDRVFP PGSKQEEVFE EIAMLVQSAL DGYPVCIFAY GQTGSGKTFT 430 440 450 460 470 480 MEGGPRGDPQ LEGLIPRAMR HLFSVAQEMS GQGWTYSFVA SYVEIYNETV RDLLATGPRK 490 500 510 520 530 540 GQGGECEIRR ASPGSEELTV TNARYVPVSC EKEVEALLHL AHQNRAVAHT AQNKRSSRSH 550 560 570 580 590 600 SVFQLQISGE HAARGLQCGA PLNLVDLAGS ERLDPGLHLG PGERDRLRET QAINSSLSTL 610 620 630 640 650 660 GLVIMALSNK ESHVPYRNSK LTYLLQNSLG GSAKMLMFVN ISPLEENVSE SLNSLRFASK 670 VNQCVIGTAQ ANKK
	Isoform 2 [UniParc]. Checksum: E74F26745611BCB4	FASTA	631	69,401
	10 20 30 40 50 60 MEDALEPAKK RTRVMGAVTK VDTSRPRGPL LSTVSQTQGH TAAQKGPKKT GPRGCSAIGT 70 80 90 100 110 120 VLRSQKPVPA APAQKPGTST APVVVGKRAG KRPAWDLKGQ LCDLNEELKR YREKTQTLEL 130 140 150 160 170 180 ENRGLREQLR EVQEQATTLG TERNTLEGEL ASVRSRAEQD QQRLETLSAR VLELEECLGT 190 200 210 220 230 240 RERLLQELQG ERLQLQEERS TLSTQLEEQE RRFQATEAAL SSSQEEVVCL RQKTEAQVTL 250 260 270 280 290 300 LAEQGDRLYG LEMERRRLHN QLQELKGNIR VFCRVRPVLE GESTPSPGFL VFPPGPAGPS 310 320 330 340 350 360 DPPTGLSLSR SDDRRSTLTG APAPTVRHDF SFDRVFPPGS KQEEVFEEIA MLVQSALDGY 370 380 390 400 410 420 PVCIFAYGQT GSGKTFTMEG GPRGDPQLEG LIPRAMRHLF SVAQEMSGQG WTYSFVASYV 430 440 450 460 470 480 EIYNETVRDL LATGPRKGQG GECEIRRASP GSEELTVTNA RYVPVSCEKE VEALLHLAHQ 490 500 510 520 530 540 NRAVAHTAQN KRSSRSHSVF QLQISGEHAA RGLQCGAPLN LVDLAGSERL DPGLHLGPGE 550 560 570 580 590 600 RDRLRETQAI NSSLSTLGLV IMALSNKESH VPYRNSKLTY LLQNSLGGSA KMLMFVNISP 610 620 630 LEENVSESLN SLRFASKVNQ CVIGTAQANK K
	Isoform 3 [UniParc]. Checksum: BF5B0CA4DE84A5C3	FASTA	611	67,117
	10 20 30 40 50 60 MEDALEPAKK RTRVMGAVTK VDTSRPRGPL LSTVSQTQGH TAAQKGPKKT GPRGCSAIGT 70 80 90 100 110 120 VLRSQKPVPA APAQKPGTST APVVVGKRAG KRPAWDLKGQ LCDLNEELKR YREKTQTLEL 130 140 150 160 170 180 ENRGLREQLR EVQEQATTLG TERNTLEGEL ASVRSRAEQT RERLLQELQG ERLQLQEERS 190 200 210 220 230 240 TLSTQLEEQE RRFQATEAAL SSSQEEVVCL RQKTEAQVTL LAEQGDRLYG LEMERRRLHN 250 260 270 280 290 300 QLQELKGNIR VFCRVRPVLE GESTPSPGFL VFPPGPAGPS DPPTGLSLSR SDDRRSTLTG 310 320 330 340 350 360 APAPTVRHDF SFDRVFPPGS KQEEVFEEIA MLVQSALDGY PVCIFAYGQT GSGKTFTMEG 370 380 390 400 410 420 GPRGDPQLEG LIPRAMRHLF SVAQEMSGQG WTYSFVASYV EIYNETVRDL LATGPRKGQG 430 440 450 460 470 480 GECEIRRASP GSEELTVTNA RYVPVSCEKE VEALLHLAHQ NRAVAHTAQN KRSSRSHSVF 490 500 510 520 530 540 QLQISGEHAA RGLQCGAPLN LVDLAGSERL DPGLHLGPGE RDRLRETQAI NSSLSTLGLV 550 560 570 580 590 600 IMALSNKESH VPYRNSKLTY LLQNSLGGSA KMLMFVNISP LEENVSESLN SLRFASKVNQ 610 CVIGTAQANK K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"KIFC2 is a novel neuron-specific C-terminal type kinesin superfamily motor for dendritic transport of multivesicular body-like organelles." Saito N., Okada Y., Noda Y., Kinoshita Y., Kondo S., Hirokawa N. Neuron 18:425-438(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY. Strain: ICR. Tissue: Brain.
[2]	"Identification of isoforms of a mitotic motor in mammalian spermatogenesis." Navolanic P.M., Sperry A.O. Biol. Reprod. 62:1360-1369(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Motor protein KIFC5A interacts with Nubp1 and Nubp2, and is implicated in the regulation of centrosome duplication." Christodoulou A., Lederer C.W., Surrey T., Vernos I., Santama N. J. Cell Sci. 119:2035-2047(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH NUBP1 AND NUBP2, DISRUPTION PHENOTYPE. Strain: C57BL/6 X SJL. Tissue: Hippocampus.
[4]	"Sequence of the mouse major histocompatibility complex class II region." Rowen L., Qin S., Madan A., Loretz C., Hall J., James R., Dors M., Shaffer T., Abbasi N., Ratcliffe A., Dickhoff R., Lasky S., Hood L. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Czech II and NMRI. Tissue: Mammary gland and Thyroid.
[6]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 97-131, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[7]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 387-674. Strain: C57BL/6J. Tissue: Head.
[8]	"Identification, partial characterization, and genetic mapping of kinesin-like protein genes in mouse." Yang Z., Hanlon D.W., Marszalek J.R., Goldstein L.S. Genomics 45:123-131(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 416-570.
[9]	"Kif5B and Kifc1 interact and are required for motility and fission of early endocytic vesicles in mouse liver." Nath S., Bananis E., Sarkar S., Stockert R.J., Sperry A.O., Murray J.W., Wolkoff A.W. Mol. Biol. Cell 18:1839-1849(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]	"Identification of a novel Leucine-rich repeat protein and candidate PP1 regulatory subunit expressed in developing spermatids." Wang R., Sperry A.O. BMC Cell Biol. 9:9-9(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PPP1R42.
[11]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35, MASS SPECTROMETRY. Tissue: Melanoma.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D49544 mRNA. Translation: BAA19676.1. Frameshift. AF221102 mRNA. Translation: AAF34646.1. AM051187 mRNA. Translation: CAJ19646.1. AF110520 Genomic DNA. Translation: AAC97970.1. BC003753 mRNA. Translation: AAH03753.1. BC057162 mRNA. Translation: AAH57162.1. BC100328 mRNA. Translation: AAI00329.1. AK081484 mRNA. Translation: BAC38230.1. AF013117 mRNA. Translation: AAC39966.1.
IPI	IPI00420324. IPI00856328. IPI00856575.
RefSeq	NP_001182227.1. NM_001195298.1. NP_444403.2. NM_053173.2.
UniGene	Mm.258953. Mm.335713.
3D structure databases
HSSP	HSSP built from PDB template 1T5C based on UniProtKB Q02224.
ProteinModelPortal	Q9QWT9.
SMR	Q9QWT9. Positions 138-207, 269-664.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9QWT9. 8 interactions.
PTM databases
PhosphoSite	Q9QWT9.
Proteomic databases
PaxDb	Q9QWT9.
PRIDE	Q9QWT9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000114361; ENSMUSP00000110001; ENSMUSG00000079553. ENSMUST00000173492; ENSMUSP00000134572; ENSMUSG00000079553.
GeneID	100502766. 16580.
KEGG	mmu:100502766. mmu:16580.
UCSC	uc008bzw.2. mouse. uc008bzx.2. mouse.
Organism-specific databases
CTD	16580. 3833.
MGI	MGI:109596. Kifc1.
Phylogenomic databases
eggNOG	COG5059.
GeneTree	ENSGT00550000074610.
HOVERGEN	HBG001080.
InParanoid	Q9QWT9.
KO	K10405.
OMA	QISGEHS.
OrthoDB	EOG412M52.
Gene expression databases
ArrayExpress	Q9QWT9.
Bgee	Q9QWT9.
CleanEx	MM_KIFC1.
Genevestigator	Q9QWT9.
Family and domain databases
Gene3D	3.40.850.10. 1 hit.
InterPro	IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. [Graphical view]
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	290117.
SOURCE	Search...

Entry information

Entry name

KIFC1_MOUSE

Accession

Primary (citable) accession number: Q9QWT9
Secondary accession number(s): O08671 , O35230, Q497Y3, Q4A1N2, Q6PG90, Q8BV06, Q99L84, Q9JKZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program