Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9QWS8

- KCNH8_RAT

UniProt

Q9QWS8 - KCNH8_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Potassium voltage-gated channel subfamily H member 8

Gene

Kcnh8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a slowly activating, outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi551 – 668118cNMPAdd
BLAST

GO - Molecular functioni

  1. phosphorelay sensor kinase activity Source: InterPro
  2. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: RGD
  3. voltage-gated potassium channel activity Source: RGD

GO - Biological processi

  1. negative regulation of apoptotic process Source: RGD
  2. potassium ion transmembrane transport Source: RefGenome
  3. regulation of membrane potential Source: RefGenome
  4. regulation of transcription from RNA polymerase II promoter Source: GOC
  5. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily H member 8
Alternative name(s):
Ether-a-go-go-like potassium channel 3
Short name:
ELK channel 3
Voltage-gated potassium channel subunit Kv12.1
Gene namesi
Name:Kcnh8
Synonyms:Elk1, Elk3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2549. Kcnh8.

Subcellular locationi

GO - Cellular componenti

  1. integral component of plasma membrane Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11021102Potassium voltage-gated channel subfamily H member 8PRO_0000054020Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9QWS8.

Expressioni

Tissue specificityi

Detected in superior cervical, mesenteric and coeliac ganglia. Expressed in brain (piriform cortex, olfactory tubercle, cerebral cortex, hippocampus pyramidial cells and dentate gyrus and basal ganglia of caudate/putamen and accumbens nucleus). Expressed in pituitary.2 Publications

Gene expression databases

GenevestigatoriQ9QWS8.

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits.

Structurei

3D structure databases

ProteinModelPortaliQ9QWS8.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 225225CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini247 – 2559ExtracellularSequence Analysis
Topological domaini277 – 29822CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini320 – 3278ExtracellularSequence Analysis
Topological domaini349 – 3535CytoplasmicSequence Analysis
Topological domaini375 – 41945ExtracellularSequence AnalysisAdd
BLAST
Topological domaini441 – 4488ExtracellularSequence Analysis
Topological domaini470 – 1102633CytoplasmicSequence AnalysisAdd
BLAST

Intramembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei420 – 44021Pore-forming; Name=Segment H5Sequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei226 – 24621Helical; Name=Segment S1Sequence AnalysisAdd
BLAST
Transmembranei256 – 27621Helical; Name=Segment S2Sequence AnalysisAdd
BLAST
Transmembranei299 – 31921Helical; Name=Segment S3Sequence AnalysisAdd
BLAST
Transmembranei328 – 34821Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
BLAST
Transmembranei354 – 37421Helical; Name=Segment S5Sequence AnalysisAdd
BLAST
Transmembranei449 – 46921Helical; Name=Segment S6Sequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 9073PASAdd
BLAST
Domaini93 – 14553PACPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi434 – 4396Selectivity filterBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi711 – 72212Poly-GluAdd
BLAST

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2202.
HOGENOMiHOG000230794.
HOVERGENiHBG052232.
InParanoidiQ9QWS8.
KOiK04911.
PhylomeDBiQ9QWS8.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR003950. K_chnl_volt-dep_ELK.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
PR01465. ELKCHANNEL.
SMARTiSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QWS8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPVMKGLLAP QNTFLDTIAT RFDGTHSNFI LANAQVAKGF PIVYCSDGFC
60 70 80 90 100
ELAGFARTEV MQKSCSCKFL FGVETNEQLM LQIEKSLEEK VEFKGEIMFY
110 120 130 140 150
KKNGAPFWCL LDIVPIKNEK GDVVLFLASF KDITDTKVKI TSEDKKEDRA
160 170 180 190 200
KGRSRAGSHF DSARRRSRAV LYHISGHLQR REKNKLKINN NVFVDKPAFP
210 220 230 240 250
EYKVSDAKKS KFILLHFSTF KAGWDWLILL ATFYVAVTVP YNVCFIGNED
260 270 280 290 300
LSTTRSTTVS DIAVEILFII DIILNFRTTY VSKSGQVIFE ARSICIHYVT
310 320 330 340 350
TWFIIDLIAA LPFDLLYAFN VTVVSLVHLL KTVRLLRLLR LLQKLDRYSQ
360 370 380 390 400
HSTIVLTLLM SMFALLAHWM ACIWYVIGKM EREDNSLLKW EVGWLHELGK
410 420 430 440 450
RLESPYYGNN TLGGPSIRSA YIAALYFTLS SLTSVGFGNV SANTDAEKIF
460 470 480 490 500
SICTMLIGAL MHALVFGNVT AIIQRMYSRW SLYHTRTKDL KDFIRVHHLP
510 520 530 540 550
QQLKQRMLEY FQTTWSVNNG IDSNELLKDF PDELRSDITM HLNKEILQLS
560 570 580 590 600
LFECASRGCL RSLSLHIKTS FCAPGEYLLR QGDALQAIYF VCSGSMEVLK
610 620 630 640 650
DSMVLAILGK GDLIGANLSI KDQVIKTNAD VKALTYCDLQ CIILKGLFEV
660 670 680 690 700
LGLYPEYAHK FVEDIQHDLT YNLREGHESD VISRLSNKST VPQAEPKGNG
710 720 730 740 750
SIKKRLPSIV EDEEEEEVEE EETTSLSPIY TRGSSVSHSK KTGSSKSYLG
760 770 780 790 800
LSLKQLTSGT VPFHSPIRVS SANSPKTKQE ADPPNHGTRK EKNLKVQLCS
810 820 830 840 850
LGTAGTPELS PRIVDGIEDG NSSEETQTFD FGSEQIRPEP RISPSLGESE
860 870 880 890 900
IGAAFLFIKA EETKQQINKL NSEVTTLTQE VSQLGKDMRS IMQLLENILS
910 920 930 940 950
PQQPSQFCSL HPTSICPSRE SFQTRVSWSA HQPCLHLQAN GAHLYHGNVT
960 970 980 990 1000
SDIWSVDPSL VGSNPQRTEA HEQSPVDSEL HHSPNLAYSP SHCQVIQEGH
1010 1020 1030 1040 1050
LQFLRCISPH SDTTLTPLQS ISATLSSSVC SSSETSLHLV LPSRSEEGSI
1060 1070 1080 1090 1100
THGPVSSFSL ENLPGSWDRE GMMSASTEPL ENFPVEVVTS TADVKDSKAI

NV
Length:1,102
Mass (Da):123,231
Last modified:November 28, 2002 - v2
Checksum:iA135CC36E2E7F1A3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711F → L in CAA07591. (PubMed:9824707)Curated
Sequence conflicti187 – 1871K → N in CAA07591. (PubMed:9824707)Curated
Sequence conflicti296 – 2961I → T in CAA07591. (PubMed:9824707)Curated
Sequence conflicti370 – 3701M → I in CAA07591. (PubMed:9824707)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF061957 mRNA. Translation: AAC61520.1.
AJ007632 mRNA. Translation: CAA07591.1.
PIRiT17367.
RefSeqiNP_659563.1. NM_145095.1.
UniGeneiRn.30029.

Genome annotation databases

GeneIDi246325.
KEGGirno:246325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF061957 mRNA. Translation: AAC61520.1 .
AJ007632 mRNA. Translation: CAA07591.1 .
PIRi T17367.
RefSeqi NP_659563.1. NM_145095.1.
UniGenei Rn.30029.

3D structure databases

ProteinModelPortali Q9QWS8.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q9QWS8.

Proteomic databases

PaxDbi Q9QWS8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 246325.
KEGGi rno:246325.

Organism-specific databases

CTDi 131096.
RGDi 2549. Kcnh8.

Phylogenomic databases

eggNOGi COG2202.
HOGENOMi HOG000230794.
HOVERGENi HBG052232.
InParanoidi Q9QWS8.
KOi K04911.
PhylomeDBi Q9QWS8.

Miscellaneous databases

NextBioi 623752.
PROi Q9QWS8.

Gene expression databases

Genevestigatori Q9QWS8.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
InterProi IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR003950. K_chnl_volt-dep_ELK.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view ]
PRINTSi PR01463. EAGCHANLFMLY.
PR01465. ELKCHANNEL.
SMARTi SM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsi TIGR00229. sensory_box. 1 hit.
PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of a mammalian elk potassium channel gene and EAG mRNA distribution in rat sympathetic ganglia."
    Shi W., Wang H.-S., Pan Z., Wymore R.S., Cohen I.S., McKinnon D., Dixon J.E.
    J. Physiol. (Lond.) 511:675-682(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and functional expression of rat ether-a-go-go-like K+ channel genes."
    Engeland B., Neu A., Ludwig J., Roeper J., Pongs O.
    J. Physiol. (Lond.) 513:647-654(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-379.
    Tissue: Brain cortex.
  3. "Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary."
    Wulfsen I., Hauber H.-P., Schiemann D., Bauer C.K., Schwarz J.R.
    J. Neuroendocrinol. 12:263-272(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Differential expression of genes encoding subthreshold-operating voltage-gated K+ channels in brain."
    Saganich M.J., Machado E., Rudy B.
    J. Neurosci. 21:4609-4624(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiKCNH8_RAT
AccessioniPrimary (citable) accession number: Q9QWS8
Secondary accession number(s): O88877
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: November 28, 2002
Last modified: October 1, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3