Potassium voltage-gated channel subfamily H member 8

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Q9QWS8 (KCNH8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 106. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily H member 8

Alternative name(s):
Ether-a-go-go-like potassium channel 3
Short name=ELK channel 3
Voltage-gated potassium channel subunit Kv12.1

Gene names

Name:	Kcnh8
Synonyms:	Elk1, Elk3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1102 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a slowly activating, outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.
Subunit structure	The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Detected in superior cervical, mesenteric and coeliac ganglia. Expressed in brain (piriform cortex, olfactory tubercle, cerebral cortex, hippocampus pyramidial cells and dentate gyrus and basal ganglia of caudate/putamen and accumbens nucleus). Expressed in pituitary. Ref.3 Ref.4
Domain	The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Sequence similarities	Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv12.1/KCNH8 sub-subfamily. [View classification] Contains 1 cyclic nucleotide-binding domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of apoptotic process Inferred from mutant phenotype PubMed 12740370. Source: RGD signal transduction by phosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from mutant phenotype PubMed 12740370. Source: RGD phosphorelay sensor kinase activity Inferred from electronic annotation. Source: InterPro voltage-gated potassium channel activity Inferred from mutant phenotype Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1102

1102

Potassium voltage-gated channel subfamily H member 8

PRO_0000054020

Regions

Topological domain

1 – 225

225

Cytoplasmic Potential

Transmembrane

226 – 246

Helical; Name=Segment S1; Potential

Topological domain

247 – 255

Extracellular Potential

Transmembrane

256 – 276

Helical; Name=Segment S2; Potential

Topological domain

277 – 298

Cytoplasmic Potential

Transmembrane

299 – 319

Helical; Name=Segment S3; Potential

Topological domain

320 – 327

Extracellular Potential

Transmembrane

328 – 348

Helical; Voltage-sensor; Name=Segment S4; Potential

Topological domain

349 – 353

Cytoplasmic Potential

Transmembrane

354 – 374

Helical; Name=Segment S5; Potential

Topological domain

375 – 419

Extracellular Potential

Intramembrane

420 – 440

Pore-forming; Name=Segment H5; Potential

Topological domain

441 – 448

Extracellular Potential

Transmembrane

449 – 469

Helical; Name=Segment S6; Potential

Topological domain

470 – 1102

633

Cytoplasmic Potential

Domain

18 – 90

PAS

Domain

93 – 145

PAC

Nucleotide binding

551 – 668

118

cNMP

Motif

434 – 439

Selectivity filter By similarity

Compositional bias

711 – 722

Poly-Glu

Amino acid modifications

Glycosylation

320

N-linked (GlcNAc...) Potential

Glycosylation

409

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

F → L in CAA07591. Ref.2

Sequence conflict

187

K → N in CAA07591. Ref.2

Sequence conflict

296

I → T in CAA07591. Ref.2

Sequence conflict

370

M → I in CAA07591. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q9QWS8 [UniParc].

Last modified November 28, 2002. Version 2.
Checksum: A135CC36E2E7F1A3
Show »

FASTA

1,102

123,231

        10         20         30         40         50         60 
MPVMKGLLAP QNTFLDTIAT RFDGTHSNFI LANAQVAKGF PIVYCSDGFC ELAGFARTEV 

        70         80         90        100        110        120 
MQKSCSCKFL FGVETNEQLM LQIEKSLEEK VEFKGEIMFY KKNGAPFWCL LDIVPIKNEK 

       130        140        150        160        170        180 
GDVVLFLASF KDITDTKVKI TSEDKKEDRA KGRSRAGSHF DSARRRSRAV LYHISGHLQR 

       190        200        210        220        230        240 
REKNKLKINN NVFVDKPAFP EYKVSDAKKS KFILLHFSTF KAGWDWLILL ATFYVAVTVP 

       250        260        270        280        290        300 
YNVCFIGNED LSTTRSTTVS DIAVEILFII DIILNFRTTY VSKSGQVIFE ARSICIHYVT 

       310        320        330        340        350        360 
TWFIIDLIAA LPFDLLYAFN VTVVSLVHLL KTVRLLRLLR LLQKLDRYSQ HSTIVLTLLM 

       370        380        390        400        410        420 
SMFALLAHWM ACIWYVIGKM EREDNSLLKW EVGWLHELGK RLESPYYGNN TLGGPSIRSA 

       430        440        450        460        470        480 
YIAALYFTLS SLTSVGFGNV SANTDAEKIF SICTMLIGAL MHALVFGNVT AIIQRMYSRW 

       490        500        510        520        530        540 
SLYHTRTKDL KDFIRVHHLP QQLKQRMLEY FQTTWSVNNG IDSNELLKDF PDELRSDITM 

       550        560        570        580        590        600 
HLNKEILQLS LFECASRGCL RSLSLHIKTS FCAPGEYLLR QGDALQAIYF VCSGSMEVLK 

       610        620        630        640        650        660 
DSMVLAILGK GDLIGANLSI KDQVIKTNAD VKALTYCDLQ CIILKGLFEV LGLYPEYAHK 

       670        680        690        700        710        720 
FVEDIQHDLT YNLREGHESD VISRLSNKST VPQAEPKGNG SIKKRLPSIV EDEEEEEVEE 

       730        740        750        760        770        780 
EETTSLSPIY TRGSSVSHSK KTGSSKSYLG LSLKQLTSGT VPFHSPIRVS SANSPKTKQE 

       790        800        810        820        830        840 
ADPPNHGTRK EKNLKVQLCS LGTAGTPELS PRIVDGIEDG NSSEETQTFD FGSEQIRPEP 

       850        860        870        880        890        900 
RISPSLGESE IGAAFLFIKA EETKQQINKL NSEVTTLTQE VSQLGKDMRS IMQLLENILS 

       910        920        930        940        950        960 
PQQPSQFCSL HPTSICPSRE SFQTRVSWSA HQPCLHLQAN GAHLYHGNVT SDIWSVDPSL 

       970        980        990       1000       1010       1020 
VGSNPQRTEA HEQSPVDSEL HHSPNLAYSP SHCQVIQEGH LQFLRCISPH SDTTLTPLQS 

      1030       1040       1050       1060       1070       1080 
ISATLSSSVC SSSETSLHLV LPSRSEEGSI THGPVSSFSL ENLPGSWDRE GMMSASTEPL 

      1090       1100 
ENFPVEVVTS TADVKDSKAI NV

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References

[1]	"Cloning of a mammalian elk potassium channel gene and EAG mRNA distribution in rat sympathetic ganglia." Shi W., Wang H.-S., Pan Z., Wymore R.S., Cohen I.S., McKinnon D., Dixon J.E. J. Physiol. (Lond.) 511:675-682(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and functional expression of rat ether-a-go-go-like K+ channel genes." Engeland B., Neu A., Ludwig J., Roeper J., Pongs O. J. Physiol. (Lond.) 513:647-654(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-379. Tissue: Brain cortex.
[3]	"Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary." Wulfsen I., Hauber H.-P., Schiemann D., Bauer C.K., Schwarz J.R. J. Neuroendocrinol. 12:263-272(2000) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[4]	"Differential expression of genes encoding subthreshold-operating voltage-gated K+ channels in brain." Saganich M.J., Machado E., Rudy B. J. Neurosci. 21:4609-4624(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF061957 mRNA. Translation: AAC61520.1. AJ007632 mRNA. Translation: CAA07591.1.
IPI	IPI00212617.
PIR	T17367.
RefSeq	NP_659563.1. NM_145095.1.
UniGene	Rn.30029.
3D structure databases
ProteinModelPortal	Q9QWS8.
ModBase	Search...
Proteomic databases
PaxDb	Q9QWS8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	246325.
KEGG	rno:246325.
Organism-specific databases
CTD	131096.
RGD	2549. Kcnh8.
Phylogenomic databases
eggNOG	COG2202.
HOGENOM	HOG000230794.
HOVERGEN	HBG052232.
InParanoid	Q9QWS8.
KO	K04911.
OrthoDB	EOG47WNN0.
Gene expression databases
ArrayExpress	Q9QWS8.
Genevestigator	Q9QWS8.
GermOnline	ENSRNOG00000012711. Rattus norvegicus.
Family and domain databases
Gene3D	2.60.120.10. 1 hit.
InterPro	IPR018490. cNMP-bd-like. IPR000595. cNMP-bd_dom. IPR005821. Ion_trans_dom. IPR003938. K_chnl_volt-dep_EAG/ELK/ERG. IPR003950. K_chnl_volt-dep_ELK. IPR001610. PAC. IPR000014. PAS. IPR000700. PAS-assoc_C. IPR014710. RmlC-like_jellyroll. [Graphical view]
Pfam	PF00027. cNMP_binding. 1 hit. PF00520. Ion_trans. 1 hit. [Graphical view]
PRINTS	PR01463. EAGCHANLFMLY. PR01465. ELKCHANNEL.
SMART	SM00100. cNMP. 1 hit. SM00086. PAC. 1 hit. [Graphical view]
SUPFAM	SSF51206. cNMP_binding. 1 hit.
TIGRFAMs	TIGR00229. sensory_box. 1 hit.
PROSITE	PS00888. CNMP_BINDING_1. False negative. PS00889. CNMP_BINDING_2. False negative. PS50042. CNMP_BINDING_3. 1 hit. PS50113. PAC. 1 hit. PS50112. PAS. False negative. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q9QWS8.
NextBio	623752.

Entry information

Entry name

KCNH8_RAT

Accession

Primary (citable) accession number: Q9QWS8
Secondary accession number(s): O88877

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	November 28, 2002
Last modified:	April 3, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents