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Q9QWS8

- KCNH8_RAT

UniProt

Q9QWS8 - KCNH8_RAT

Protein

Potassium voltage-gated channel subfamily H member 8

Gene

Kcnh8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (28 Nov 2002)
      Previous versions | rss
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    Functioni

    Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a slowly activating, outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi551 – 668118cNMPAdd
    BLAST

    GO - Molecular functioni

    1. phosphorelay sensor kinase activity Source: InterPro
    2. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: RGD
    3. voltage-gated potassium channel activity Source: RGD

    GO - Biological processi

    1. negative regulation of apoptotic process Source: RGD
    2. potassium ion transmembrane transport Source: RefGenome
    3. regulation of membrane potential Source: RefGenome
    4. regulation of transcription from RNA polymerase II promoter Source: GOC
    5. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium voltage-gated channel subfamily H member 8
    Alternative name(s):
    Ether-a-go-go-like potassium channel 3
    Short name:
    ELK channel 3
    Voltage-gated potassium channel subunit Kv12.1
    Gene namesi
    Name:Kcnh8
    Synonyms:Elk1, Elk3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2549. Kcnh8.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: RefGenome

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11021102Potassium voltage-gated channel subfamily H member 8PRO_0000054020Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi320 – 3201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi409 – 4091N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9QWS8.

    Expressioni

    Tissue specificityi

    Detected in superior cervical, mesenteric and coeliac ganglia. Expressed in brain (piriform cortex, olfactory tubercle, cerebral cortex, hippocampus pyramidial cells and dentate gyrus and basal ganglia of caudate/putamen and accumbens nucleus). Expressed in pituitary.2 Publications

    Gene expression databases

    GenevestigatoriQ9QWS8.

    Interactioni

    Subunit structurei

    The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QWS8.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 225225CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini247 – 2559ExtracellularSequence Analysis
    Topological domaini277 – 29822CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini320 – 3278ExtracellularSequence Analysis
    Topological domaini349 – 3535CytoplasmicSequence Analysis
    Topological domaini375 – 41945ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini441 – 4488ExtracellularSequence Analysis
    Topological domaini470 – 1102633CytoplasmicSequence AnalysisAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei420 – 44021Pore-forming; Name=Segment H5Sequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei226 – 24621Helical; Name=Segment S1Sequence AnalysisAdd
    BLAST
    Transmembranei256 – 27621Helical; Name=Segment S2Sequence AnalysisAdd
    BLAST
    Transmembranei299 – 31921Helical; Name=Segment S3Sequence AnalysisAdd
    BLAST
    Transmembranei328 – 34821Helical; Voltage-sensor; Name=Segment S4Sequence AnalysisAdd
    BLAST
    Transmembranei354 – 37421Helical; Name=Segment S5Sequence AnalysisAdd
    BLAST
    Transmembranei449 – 46921Helical; Name=Segment S6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 9073PASAdd
    BLAST
    Domaini93 – 14553PACPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi434 – 4396Selectivity filterBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi711 – 72212Poly-GluAdd
    BLAST

    Domaini

    The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
    Contains 1 PAS (PER-ARNT-SIM) domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2202.
    HOGENOMiHOG000230794.
    HOVERGENiHBG052232.
    InParanoidiQ9QWS8.
    KOiK04911.
    PhylomeDBiQ9QWS8.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
    IPR003950. K_chnl_volt-dep_ELK.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF13426. PAS_9. 1 hit.
    [Graphical view]
    PRINTSiPR01463. EAGCHANLFMLY.
    PR01465. ELKCHANNEL.
    SMARTiSM00100. cNMP. 1 hit.
    SM00086. PAC. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    SSF55785. SSF55785. 1 hit.
    TIGRFAMsiTIGR00229. sensory_box. 1 hit.
    PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
    PS50113. PAC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9QWS8-1 [UniParc]FASTAAdd to Basket

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    MPVMKGLLAP QNTFLDTIAT RFDGTHSNFI LANAQVAKGF PIVYCSDGFC     50
    ELAGFARTEV MQKSCSCKFL FGVETNEQLM LQIEKSLEEK VEFKGEIMFY 100
    KKNGAPFWCL LDIVPIKNEK GDVVLFLASF KDITDTKVKI TSEDKKEDRA 150
    KGRSRAGSHF DSARRRSRAV LYHISGHLQR REKNKLKINN NVFVDKPAFP 200
    EYKVSDAKKS KFILLHFSTF KAGWDWLILL ATFYVAVTVP YNVCFIGNED 250
    LSTTRSTTVS DIAVEILFII DIILNFRTTY VSKSGQVIFE ARSICIHYVT 300
    TWFIIDLIAA LPFDLLYAFN VTVVSLVHLL KTVRLLRLLR LLQKLDRYSQ 350
    HSTIVLTLLM SMFALLAHWM ACIWYVIGKM EREDNSLLKW EVGWLHELGK 400
    RLESPYYGNN TLGGPSIRSA YIAALYFTLS SLTSVGFGNV SANTDAEKIF 450
    SICTMLIGAL MHALVFGNVT AIIQRMYSRW SLYHTRTKDL KDFIRVHHLP 500
    QQLKQRMLEY FQTTWSVNNG IDSNELLKDF PDELRSDITM HLNKEILQLS 550
    LFECASRGCL RSLSLHIKTS FCAPGEYLLR QGDALQAIYF VCSGSMEVLK 600
    DSMVLAILGK GDLIGANLSI KDQVIKTNAD VKALTYCDLQ CIILKGLFEV 650
    LGLYPEYAHK FVEDIQHDLT YNLREGHESD VISRLSNKST VPQAEPKGNG 700
    SIKKRLPSIV EDEEEEEVEE EETTSLSPIY TRGSSVSHSK KTGSSKSYLG 750
    LSLKQLTSGT VPFHSPIRVS SANSPKTKQE ADPPNHGTRK EKNLKVQLCS 800
    LGTAGTPELS PRIVDGIEDG NSSEETQTFD FGSEQIRPEP RISPSLGESE 850
    IGAAFLFIKA EETKQQINKL NSEVTTLTQE VSQLGKDMRS IMQLLENILS 900
    PQQPSQFCSL HPTSICPSRE SFQTRVSWSA HQPCLHLQAN GAHLYHGNVT 950
    SDIWSVDPSL VGSNPQRTEA HEQSPVDSEL HHSPNLAYSP SHCQVIQEGH 1000
    LQFLRCISPH SDTTLTPLQS ISATLSSSVC SSSETSLHLV LPSRSEEGSI 1050
    THGPVSSFSL ENLPGSWDRE GMMSASTEPL ENFPVEVVTS TADVKDSKAI 1100
    NV 1102
    Length:1,102
    Mass (Da):123,231
    Last modified:November 28, 2002 - v2
    Checksum:iA135CC36E2E7F1A3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti71 – 711F → L in CAA07591. (PubMed:9824707)Curated
    Sequence conflicti187 – 1871K → N in CAA07591. (PubMed:9824707)Curated
    Sequence conflicti296 – 2961I → T in CAA07591. (PubMed:9824707)Curated
    Sequence conflicti370 – 3701M → I in CAA07591. (PubMed:9824707)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061957 mRNA. Translation: AAC61520.1.
    AJ007632 mRNA. Translation: CAA07591.1.
    PIRiT17367.
    RefSeqiNP_659563.1. NM_145095.1.
    UniGeneiRn.30029.

    Genome annotation databases

    GeneIDi246325.
    KEGGirno:246325.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061957 mRNA. Translation: AAC61520.1 .
    AJ007632 mRNA. Translation: CAA07591.1 .
    PIRi T17367.
    RefSeqi NP_659563.1. NM_145095.1.
    UniGenei Rn.30029.

    3D structure databases

    ProteinModelPortali Q9QWS8.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q9QWS8.

    Proteomic databases

    PaxDbi Q9QWS8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 246325.
    KEGGi rno:246325.

    Organism-specific databases

    CTDi 131096.
    RGDi 2549. Kcnh8.

    Phylogenomic databases

    eggNOGi COG2202.
    HOGENOMi HOG000230794.
    HOVERGENi HBG052232.
    InParanoidi Q9QWS8.
    KOi K04911.
    PhylomeDBi Q9QWS8.

    Miscellaneous databases

    NextBioi 623752.
    PROi Q9QWS8.

    Gene expression databases

    Genevestigatori Q9QWS8.

    Family and domain databases

    Gene3Di 2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
    IPR003950. K_chnl_volt-dep_ELK.
    IPR001610. PAC.
    IPR000014. PAS.
    IPR000700. PAS-assoc_C.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    PF13426. PAS_9. 1 hit.
    [Graphical view ]
    PRINTSi PR01463. EAGCHANLFMLY.
    PR01465. ELKCHANNEL.
    SMARTi SM00100. cNMP. 1 hit.
    SM00086. PAC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    SSF55785. SSF55785. 1 hit.
    TIGRFAMsi TIGR00229. sensory_box. 1 hit.
    PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
    PS50113. PAC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a mammalian elk potassium channel gene and EAG mRNA distribution in rat sympathetic ganglia."
      Shi W., Wang H.-S., Pan Z., Wymore R.S., Cohen I.S., McKinnon D., Dixon J.E.
      J. Physiol. (Lond.) 511:675-682(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and functional expression of rat ether-a-go-go-like K+ channel genes."
      Engeland B., Neu A., Ludwig J., Roeper J., Pongs O.
      J. Physiol. (Lond.) 513:647-654(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-379.
      Tissue: Brain cortex.
    3. "Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary."
      Wulfsen I., Hauber H.-P., Schiemann D., Bauer C.K., Schwarz J.R.
      J. Neuroendocrinol. 12:263-272(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "Differential expression of genes encoding subthreshold-operating voltage-gated K+ channels in brain."
      Saganich M.J., Machado E., Rudy B.
      J. Neurosci. 21:4609-4624(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiKCNH8_RAT
    AccessioniPrimary (citable) accession number: Q9QWS8
    Secondary accession number(s): O88877
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2002
    Last sequence update: November 28, 2002
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3