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Q9QWS8 (KCNH8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily H member 8
Alternative name(s):
Ether-a-go-go-like potassium channel 3
Short name=ELK channel 3
Voltage-gated potassium channel subunit Kv12.1
Gene names
Name:Kcnh8
Synonyms:Elk1, Elk3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1102 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a slowly activating, outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.

Subunit structure

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Detected in superior cervical, mesenteric and coeliac ganglia. Expressed in brain (piriform cortex, olfactory tubercle, cerebral cortex, hippocampus pyramidial cells and dentate gyrus and basal ganglia of caudate/putamen and accumbens nucleus). Expressed in pituitary. Ref.3 Ref.4

Domain

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similarities

Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv12.1/KCNH8 sub-subfamily. [View classification]

Contains 1 cyclic nucleotide-binding domain.

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11021102Potassium voltage-gated channel subfamily H member 8
PRO_0000054020

Regions

Topological domain1 – 225225Cytoplasmic Potential
Transmembrane226 – 24621Helical; Name=Segment S1; Potential
Topological domain247 – 2559Extracellular Potential
Transmembrane256 – 27621Helical; Name=Segment S2; Potential
Topological domain277 – 29822Cytoplasmic Potential
Transmembrane299 – 31921Helical; Name=Segment S3; Potential
Topological domain320 – 3278Extracellular Potential
Transmembrane328 – 34821Helical; Voltage-sensor; Name=Segment S4; Potential
Topological domain349 – 3535Cytoplasmic Potential
Transmembrane354 – 37421Helical; Name=Segment S5; Potential
Topological domain375 – 41945Extracellular Potential
Intramembrane420 – 44021Pore-forming; Name=Segment H5; Potential
Topological domain441 – 4488Extracellular Potential
Transmembrane449 – 46921Helical; Name=Segment S6; Potential
Topological domain470 – 1102633Cytoplasmic Potential
Domain18 – 9073PAS
Domain93 – 14553PAC
Nucleotide binding551 – 668118cNMP
Motif434 – 4396Selectivity filter By similarity
Compositional bias711 – 72212Poly-Glu

Amino acid modifications

Glycosylation3201N-linked (GlcNAc...) Potential
Glycosylation4091N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict711F → L in CAA07591. Ref.2
Sequence conflict1871K → N in CAA07591. Ref.2
Sequence conflict2961I → T in CAA07591. Ref.2
Sequence conflict3701M → I in CAA07591. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9QWS8 [UniParc].

Last modified November 28, 2002. Version 2.
Checksum: A135CC36E2E7F1A3

FASTA1,102123,231
        10         20         30         40         50         60 
MPVMKGLLAP QNTFLDTIAT RFDGTHSNFI LANAQVAKGF PIVYCSDGFC ELAGFARTEV 

        70         80         90        100        110        120 
MQKSCSCKFL FGVETNEQLM LQIEKSLEEK VEFKGEIMFY KKNGAPFWCL LDIVPIKNEK 

       130        140        150        160        170        180 
GDVVLFLASF KDITDTKVKI TSEDKKEDRA KGRSRAGSHF DSARRRSRAV LYHISGHLQR 

       190        200        210        220        230        240 
REKNKLKINN NVFVDKPAFP EYKVSDAKKS KFILLHFSTF KAGWDWLILL ATFYVAVTVP 

       250        260        270        280        290        300 
YNVCFIGNED LSTTRSTTVS DIAVEILFII DIILNFRTTY VSKSGQVIFE ARSICIHYVT 

       310        320        330        340        350        360 
TWFIIDLIAA LPFDLLYAFN VTVVSLVHLL KTVRLLRLLR LLQKLDRYSQ HSTIVLTLLM 

       370        380        390        400        410        420 
SMFALLAHWM ACIWYVIGKM EREDNSLLKW EVGWLHELGK RLESPYYGNN TLGGPSIRSA 

       430        440        450        460        470        480 
YIAALYFTLS SLTSVGFGNV SANTDAEKIF SICTMLIGAL MHALVFGNVT AIIQRMYSRW 

       490        500        510        520        530        540 
SLYHTRTKDL KDFIRVHHLP QQLKQRMLEY FQTTWSVNNG IDSNELLKDF PDELRSDITM 

       550        560        570        580        590        600 
HLNKEILQLS LFECASRGCL RSLSLHIKTS FCAPGEYLLR QGDALQAIYF VCSGSMEVLK 

       610        620        630        640        650        660 
DSMVLAILGK GDLIGANLSI KDQVIKTNAD VKALTYCDLQ CIILKGLFEV LGLYPEYAHK 

       670        680        690        700        710        720 
FVEDIQHDLT YNLREGHESD VISRLSNKST VPQAEPKGNG SIKKRLPSIV EDEEEEEVEE 

       730        740        750        760        770        780 
EETTSLSPIY TRGSSVSHSK KTGSSKSYLG LSLKQLTSGT VPFHSPIRVS SANSPKTKQE 

       790        800        810        820        830        840 
ADPPNHGTRK EKNLKVQLCS LGTAGTPELS PRIVDGIEDG NSSEETQTFD FGSEQIRPEP 

       850        860        870        880        890        900 
RISPSLGESE IGAAFLFIKA EETKQQINKL NSEVTTLTQE VSQLGKDMRS IMQLLENILS 

       910        920        930        940        950        960 
PQQPSQFCSL HPTSICPSRE SFQTRVSWSA HQPCLHLQAN GAHLYHGNVT SDIWSVDPSL 

       970        980        990       1000       1010       1020 
VGSNPQRTEA HEQSPVDSEL HHSPNLAYSP SHCQVIQEGH LQFLRCISPH SDTTLTPLQS 

      1030       1040       1050       1060       1070       1080 
ISATLSSSVC SSSETSLHLV LPSRSEEGSI THGPVSSFSL ENLPGSWDRE GMMSASTEPL 

      1090       1100 
ENFPVEVVTS TADVKDSKAI NV 

« Hide

References

[1]"Cloning of a mammalian elk potassium channel gene and EAG mRNA distribution in rat sympathetic ganglia."
Shi W., Wang H.-S., Pan Z., Wymore R.S., Cohen I.S., McKinnon D., Dixon J.E.
J. Physiol. (Lond.) 511:675-682(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning and functional expression of rat ether-a-go-go-like K+ channel genes."
Engeland B., Neu A., Ludwig J., Roeper J., Pongs O.
J. Physiol. (Lond.) 513:647-654(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-379.
Tissue: Brain cortex.
[3]"Expression of mRNA for voltage-dependent and inward-rectifying K channels in GH3/B6 cells and rat pituitary."
Wulfsen I., Hauber H.-P., Schiemann D., Bauer C.K., Schwarz J.R.
J. Neuroendocrinol. 12:263-272(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"Differential expression of genes encoding subthreshold-operating voltage-gated K+ channels in brain."
Saganich M.J., Machado E., Rudy B.
J. Neurosci. 21:4609-4624(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF061957 mRNA. Translation: AAC61520.1.
AJ007632 mRNA. Translation: CAA07591.1.
PIRT17367.
RefSeqNP_659563.1. NM_145095.1.
UniGeneRn.30029.

3D structure databases

ProteinModelPortalQ9QWS8.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBQ9QWS8.

Proteomic databases

PaxDbQ9QWS8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID246325.
KEGGrno:246325.

Organism-specific databases

CTD131096.
RGD2549. Kcnh8.

Phylogenomic databases

eggNOGCOG2202.
HOGENOMHOG000230794.
HOVERGENHBG052232.
InParanoidQ9QWS8.
KOK04911.
PhylomeDBQ9QWS8.

Gene expression databases

GenevestigatorQ9QWS8.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR003950. K_chnl_volt-dep_ELK.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSPR01463. EAGCHANLFMLY.
PR01465. ELKCHANNEL.
SMARTSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsTIGR00229. sensory_box. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio623752.
PROQ9QWS8.

Entry information

Entry nameKCNH8_RAT
AccessionPrimary (citable) accession number: Q9QWS8
Secondary accession number(s): O88877
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: November 28, 2002
Last modified: April 16, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families