Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium voltage-gated channel subfamily H member 8

Gene

Kcnh8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a slowly activating, outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi551 – 668cNMPAdd BLAST118

GO - Molecular functioni

  • phosphorelay sensor kinase activity Source: InterPro
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: RGD
  • voltage-gated potassium channel activity Source: RGD

GO - Biological processi

  • negative regulation of apoptotic process Source: RGD
  • regulation of membrane potential Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily H member 8
Alternative name(s):
Ether-a-go-go-like potassium channel 3
Short name:
ELK channel 3
Voltage-gated potassium channel subunit Kv12.1
Gene namesi
Name:Kcnh8
Synonyms:Elk1, Elk3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2549. Kcnh8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 225CytoplasmicSequence analysisAdd BLAST225
Transmembranei226 – 246Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini247 – 255ExtracellularSequence analysis9
Transmembranei256 – 276Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini277 – 298CytoplasmicSequence analysisAdd BLAST22
Transmembranei299 – 319Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini320 – 327ExtracellularSequence analysis8
Transmembranei328 – 348Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini349 – 353CytoplasmicSequence analysis5
Transmembranei354 – 374Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini375 – 419ExtracellularSequence analysisAdd BLAST45
Intramembranei420 – 440Pore-forming; Name=Segment H5Sequence analysisAdd BLAST21
Topological domaini441 – 448ExtracellularSequence analysis8
Transmembranei449 – 469Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini470 – 1102CytoplasmicSequence analysisAdd BLAST633

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000540201 – 1102Potassium voltage-gated channel subfamily H member 8Add BLAST1102

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi320N-linked (GlcNAc...)Sequence analysis1
Glycosylationi409N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9QWS8.
PRIDEiQ9QWS8.

Expressioni

Tissue specificityi

Detected in superior cervical, mesenteric and coeliac ganglia. Expressed in brain (piriform cortex, olfactory tubercle, cerebral cortex, hippocampus pyramidial cells and dentate gyrus and basal ganglia of caudate/putamen and accumbens nucleus). Expressed in pituitary.2 Publications

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming alpha subunits that can associate with modulating beta subunits.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017246.

Chemistry databases

BindingDBiQ9QWS8.

Structurei

3D structure databases

ProteinModelPortaliQ9QWS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 90PASAdd BLAST73
Domaini93 – 145PACPROSITE-ProRule annotationAdd BLAST53

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi434 – 439Selectivity filterBy similarity6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi711 – 722Poly-GluAdd BLAST12

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000230794.
HOVERGENiHBG052232.
InParanoidiQ9QWS8.
KOiK04911.
PhylomeDBiQ9QWS8.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR003950. K_chnl_volt-dep_ELK.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
PR01465. ELKCHANNEL.
SMARTiSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QWS8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVMKGLLAP QNTFLDTIAT RFDGTHSNFI LANAQVAKGF PIVYCSDGFC
60 70 80 90 100
ELAGFARTEV MQKSCSCKFL FGVETNEQLM LQIEKSLEEK VEFKGEIMFY
110 120 130 140 150
KKNGAPFWCL LDIVPIKNEK GDVVLFLASF KDITDTKVKI TSEDKKEDRA
160 170 180 190 200
KGRSRAGSHF DSARRRSRAV LYHISGHLQR REKNKLKINN NVFVDKPAFP
210 220 230 240 250
EYKVSDAKKS KFILLHFSTF KAGWDWLILL ATFYVAVTVP YNVCFIGNED
260 270 280 290 300
LSTTRSTTVS DIAVEILFII DIILNFRTTY VSKSGQVIFE ARSICIHYVT
310 320 330 340 350
TWFIIDLIAA LPFDLLYAFN VTVVSLVHLL KTVRLLRLLR LLQKLDRYSQ
360 370 380 390 400
HSTIVLTLLM SMFALLAHWM ACIWYVIGKM EREDNSLLKW EVGWLHELGK
410 420 430 440 450
RLESPYYGNN TLGGPSIRSA YIAALYFTLS SLTSVGFGNV SANTDAEKIF
460 470 480 490 500
SICTMLIGAL MHALVFGNVT AIIQRMYSRW SLYHTRTKDL KDFIRVHHLP
510 520 530 540 550
QQLKQRMLEY FQTTWSVNNG IDSNELLKDF PDELRSDITM HLNKEILQLS
560 570 580 590 600
LFECASRGCL RSLSLHIKTS FCAPGEYLLR QGDALQAIYF VCSGSMEVLK
610 620 630 640 650
DSMVLAILGK GDLIGANLSI KDQVIKTNAD VKALTYCDLQ CIILKGLFEV
660 670 680 690 700
LGLYPEYAHK FVEDIQHDLT YNLREGHESD VISRLSNKST VPQAEPKGNG
710 720 730 740 750
SIKKRLPSIV EDEEEEEVEE EETTSLSPIY TRGSSVSHSK KTGSSKSYLG
760 770 780 790 800
LSLKQLTSGT VPFHSPIRVS SANSPKTKQE ADPPNHGTRK EKNLKVQLCS
810 820 830 840 850
LGTAGTPELS PRIVDGIEDG NSSEETQTFD FGSEQIRPEP RISPSLGESE
860 870 880 890 900
IGAAFLFIKA EETKQQINKL NSEVTTLTQE VSQLGKDMRS IMQLLENILS
910 920 930 940 950
PQQPSQFCSL HPTSICPSRE SFQTRVSWSA HQPCLHLQAN GAHLYHGNVT
960 970 980 990 1000
SDIWSVDPSL VGSNPQRTEA HEQSPVDSEL HHSPNLAYSP SHCQVIQEGH
1010 1020 1030 1040 1050
LQFLRCISPH SDTTLTPLQS ISATLSSSVC SSSETSLHLV LPSRSEEGSI
1060 1070 1080 1090 1100
THGPVSSFSL ENLPGSWDRE GMMSASTEPL ENFPVEVVTS TADVKDSKAI

NV
Length:1,102
Mass (Da):123,231
Last modified:November 28, 2002 - v2
Checksum:iA135CC36E2E7F1A3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71F → L in CAA07591 (PubMed:9824707).Curated1
Sequence conflicti187K → N in CAA07591 (PubMed:9824707).Curated1
Sequence conflicti296I → T in CAA07591 (PubMed:9824707).Curated1
Sequence conflicti370M → I in CAA07591 (PubMed:9824707).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061957 mRNA. Translation: AAC61520.1.
AJ007632 mRNA. Translation: CAA07591.1.
PIRiT17367.
RefSeqiNP_659563.1. NM_145095.2.
UniGeneiRn.30029.

Genome annotation databases

GeneIDi246325.
KEGGirno:246325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061957 mRNA. Translation: AAC61520.1.
AJ007632 mRNA. Translation: CAA07591.1.
PIRiT17367.
RefSeqiNP_659563.1. NM_145095.2.
UniGeneiRn.30029.

3D structure databases

ProteinModelPortaliQ9QWS8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017246.

Chemistry databases

BindingDBiQ9QWS8.

Proteomic databases

PaxDbiQ9QWS8.
PRIDEiQ9QWS8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi246325.
KEGGirno:246325.

Organism-specific databases

CTDi131096.
RGDi2549. Kcnh8.

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000230794.
HOVERGENiHBG052232.
InParanoidiQ9QWS8.
KOiK04911.
PhylomeDBiQ9QWS8.

Miscellaneous databases

PROiQ9QWS8.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR003950. K_chnl_volt-dep_ELK.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
PR01465. ELKCHANNEL.
SMARTiSM00100. cNMP. 1 hit.
SM00086. PAC. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
SSF55785. SSF55785. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS50113. PAC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKCNH8_RAT
AccessioniPrimary (citable) accession number: Q9QWS8
Secondary accession number(s): O88877
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: November 28, 2002
Last modified: October 5, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.