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Protein

Alpha-N-acetylgalactosaminidase

Gene

Naga

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids (By similarity).By similarity

Catalytic activityi

Cleavage of non-reducing alpha-(1->3)-N-acetylgalactosamine residues from human blood group A and AB mucin glycoproteins, Forssman hapten and blood group A lacto series glycolipids.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei154SubstrateBy similarity1
Active sitei156NucleophileBy similarity1
Binding sitei188SubstrateBy similarity1
Binding sitei213SubstrateBy similarity1
Active sitei217Proton donorBy similarity1
Binding sitei217SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.49 3474

Protein family/group databases

CAZyiGH27 Glycoside Hydrolase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-N-acetylgalactosaminidase (EC:3.2.1.49)
Alternative name(s):
Alpha-galactosidase B
Gene namesi
Name:Naga
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1261422 Naga

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17By similarityAdd BLAST17
ChainiPRO_000000101918 – 415Alpha-N-acetylgalactosaminidaseAdd BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi38 ↔ 80By similarity
Disulfide bondi42 ↔ 49By similarity
Disulfide bondi127 ↔ 158By similarity
Glycosylationi177N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi187 ↔ 209By similarity
Glycosylationi201N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei322PhosphoserineBy similarity1
Modified residuei332PhosphoserineBy similarity1
Glycosylationi385N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ9QWR8
MaxQBiQ9QWR8
PaxDbiQ9QWR8
PRIDEiQ9QWR8

PTM databases

PhosphoSitePlusiQ9QWR8
SwissPalmiQ9QWR8

Expressioni

Gene expression databases

BgeeiENSMUSG00000022453
CleanExiMM_NAGA
GenevisibleiQ9QWR8 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9QWR8 1 interactor.
MINTiQ9QWR8
STRINGi10090.ENSMUSP00000023088

Structurei

3D structure databases

ProteinModelPortaliQ9QWR8
SMRiQ9QWR8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 79Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2366 Eukaryota
ENOG410XPF1 LUCA
GeneTreeiENSGT00390000008751
HOGENOMiHOG000161224
HOVERGENiHBG001989
InParanoidiQ9QWR8
KOiK01204
OMAiDDLWDRW
OrthoDBiEOG091G0BGV
PhylomeDBiQ9QWR8
TreeFamiTF312909

Family and domain databases

CDDicd14792 GH27, 1 hit
Gene3Di2.60.40.11801 hit
3.20.20.701 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR002241 Glyco_hydro_27
IPR000111 Glyco_hydro_27/36_CS
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR035373 Melibiase/NAGA_C
PfamiView protein in Pfam
PF16499 Melibiase_2, 1 hit
PF17450 Melibiase_2_C, 1 hit
PRINTSiPR00740 GLHYDRLASE27
SUPFAMiSSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS00512 ALPHA_GALACTOSIDASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QWR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQKTVLLLA LVAQVLMLEN GLLRTPPMGW LAWERFRCNI DCVEDPKNCI
60 70 80 90 100
SERLFMEMAD RLAQDGWRDL GYVYLNIDDC WIGGRDASGR LIPDPKRFPH
110 120 130 140 150
GIAFLADYAH SLGLKLGIYE DMGKMTCMGY PGTTLDKVEL DAETFAEWKV
160 170 180 190 200
DMLKLDGCFS SSRERAEGYP KMAAALNATG RPIAFSCSWP AYEGGLPPKV
210 220 230 240 250
NYTEVSRVCN LWRNYKDIQD SWKSVLSILD WFVRHQDVLQ PVAGPGHWND
260 270 280 290 300
PDMLLIGNFG LSFDESRAQM ALWTVLAAPL LMSTDLRTIS PQNMDILQNP
310 320 330 340 350
LMIKINQDPL GIQGRRILKS KSHIEVFKRY LSNQASALVF FSRRTDMPFR
360 370 380 390 400
FHCSLLELNY PKGRVYEGQN VFTGDIFSGL QTEVNFTVII NPSGVVMWYL
410
YPIKDLGIST MMSHW
Length:415
Mass (Da):47,235
Last modified:June 7, 2005 - v2
Checksum:i8BBA9BE03E1350C8
GO

Sequence cautioni

The sequence AAL87527 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52 – 53ER → DG in CAA11703 (PubMed:9602128).Curated2
Sequence conflicti239L → P in AAH21631 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF079458 mRNA Translation: AAC28851.1
AJ223966 Genomic DNA Translation: CAA11703.1
AY079439 Genomic DNA Translation: AAL87527.1 Different initiation.
AY079440 Genomic DNA Translation: AAL87528.1
AK077116 mRNA Translation: BAC36620.1
BC021631 mRNA Translation: AAH21631.1
CCDSiCCDS27686.1
RefSeqiNP_032695.3, NM_008669.4
UniGeneiMm.20325

Genome annotation databases

EnsembliENSMUST00000023088; ENSMUSP00000023088; ENSMUSG00000022453
GeneIDi17939
KEGGimmu:17939
UCSCiuc007wyw.2 mouse

Similar proteinsi

Entry informationi

Entry nameiNAGAB_MOUSE
AccessioniPrimary (citable) accession number: Q9QWR8
Secondary accession number(s): O88620, Q8R437, Q8VDK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: February 28, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome