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Q9QWR8

- NAGAB_MOUSE

UniProt

Q9QWR8 - NAGAB_MOUSE

Protein

Alpha-N-acetylgalactosaminidase

Gene

Naga

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids By similarity.By similarity

    Catalytic activityi

    Cleavage of non-reducing alpha-(1->3)-N-acetylgalactosamine residues from human blood group A and AB mucin glycoproteins, Forssman hapten and blood group A lacto series glycolipids.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei154 – 1541SubstrateBy similarity
    Active sitei156 – 1561NucleophileBy similarity
    Binding sitei188 – 1881SubstrateBy similarity
    Binding sitei213 – 2131SubstrateBy similarity
    Active sitei217 – 2171Proton donorBy similarity
    Binding sitei217 – 2171SubstrateBy similarity

    GO - Molecular functioni

    1. alpha-galactosidase activity Source: RefGenome
    2. alpha-N-acetylgalactosaminidase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate catabolic process Source: UniProtKB
    2. glycolipid catabolic process Source: UniProtKB
    3. glycoside catabolic process Source: RefGenome
    4. glycosylceramide catabolic process Source: RefGenome
    5. oligosaccharide metabolic process Source: RefGenome

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH27. Glycoside Hydrolase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-N-acetylgalactosaminidase (EC:3.2.1.49)
    Alternative name(s):
    Alpha-galactosidase B
    Gene namesi
    Name:Naga
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1261422. Naga.

    Subcellular locationi

    Lysosome By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717By similarityAdd
    BLAST
    Chaini18 – 415398Alpha-N-acetylgalactosaminidasePRO_0000001019Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi38 ↔ 80By similarity
    Disulfide bondi42 ↔ 49By similarity
    Disulfide bondi127 ↔ 158By similarity
    Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi187 ↔ 209By similarity
    Glycosylationi201 – 2011N-linked (GlcNAc...)Sequence Analysis
    Modified residuei322 – 3221PhosphoserineBy similarity
    Modified residuei332 – 3321PhosphoserineBy similarity
    Glycosylationi385 – 3851N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ9QWR8.
    PaxDbiQ9QWR8.
    PRIDEiQ9QWR8.

    PTM databases

    PhosphoSiteiQ9QWR8.

    Expressioni

    Gene expression databases

    BgeeiQ9QWR8.
    CleanExiMM_NAGA.
    GenevestigatoriQ9QWR8.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiQ9QWR8. 1 interaction.
    MINTiMINT-1849631.
    STRINGi10090.ENSMUSP00000023088.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QWR8.
    SMRiQ9QWR8. Positions 18-404.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni78 – 792Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 27 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG68897.
    GeneTreeiENSGT00390000008751.
    HOGENOMiHOG000161224.
    HOVERGENiHBG001989.
    InParanoidiQ9QWR8.
    KOiK01204.
    OMAiTNFTVVI.
    OrthoDBiEOG7F24SV.
    PhylomeDBiQ9QWR8.
    TreeFamiTF312909.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02065. Melibiase. 1 hit.
    [Graphical view]
    PRINTSiPR00740. GLHYDRLASE27.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9QWR8-1 [UniParc]FASTAAdd to Basket

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    MLQKTVLLLA LVAQVLMLEN GLLRTPPMGW LAWERFRCNI DCVEDPKNCI    50
    SERLFMEMAD RLAQDGWRDL GYVYLNIDDC WIGGRDASGR LIPDPKRFPH 100
    GIAFLADYAH SLGLKLGIYE DMGKMTCMGY PGTTLDKVEL DAETFAEWKV 150
    DMLKLDGCFS SSRERAEGYP KMAAALNATG RPIAFSCSWP AYEGGLPPKV 200
    NYTEVSRVCN LWRNYKDIQD SWKSVLSILD WFVRHQDVLQ PVAGPGHWND 250
    PDMLLIGNFG LSFDESRAQM ALWTVLAAPL LMSTDLRTIS PQNMDILQNP 300
    LMIKINQDPL GIQGRRILKS KSHIEVFKRY LSNQASALVF FSRRTDMPFR 350
    FHCSLLELNY PKGRVYEGQN VFTGDIFSGL QTEVNFTVII NPSGVVMWYL 400
    YPIKDLGIST MMSHW 415
    Length:415
    Mass (Da):47,235
    Last modified:June 7, 2005 - v2
    Checksum:i8BBA9BE03E1350C8
    GO

    Sequence cautioni

    The sequence AAL87527.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 532ER → DG in CAA11703. (PubMed:9602128)Curated
    Sequence conflicti239 – 2391L → P in AAH21631. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF079458 mRNA. Translation: AAC28851.1.
    AJ223966 Genomic DNA. Translation: CAA11703.1.
    AY079439 Genomic DNA. Translation: AAL87527.1. Different initiation.
    AY079440 Genomic DNA. Translation: AAL87528.1.
    AK077116 mRNA. Translation: BAC36620.1.
    BC021631 mRNA. Translation: AAH21631.1.
    CCDSiCCDS27686.1.
    RefSeqiNP_032695.3. NM_008669.4.
    UniGeneiMm.20325.

    Genome annotation databases

    EnsembliENSMUST00000023088; ENSMUSP00000023088; ENSMUSG00000022453.
    GeneIDi17939.
    KEGGimmu:17939.
    UCSCiuc007wyw.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF079458 mRNA. Translation: AAC28851.1 .
    AJ223966 Genomic DNA. Translation: CAA11703.1 .
    AY079439 Genomic DNA. Translation: AAL87527.1 . Different initiation.
    AY079440 Genomic DNA. Translation: AAL87528.1 .
    AK077116 mRNA. Translation: BAC36620.1 .
    BC021631 mRNA. Translation: AAH21631.1 .
    CCDSi CCDS27686.1.
    RefSeqi NP_032695.3. NM_008669.4.
    UniGenei Mm.20325.

    3D structure databases

    ProteinModelPortali Q9QWR8.
    SMRi Q9QWR8. Positions 18-404.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9QWR8. 1 interaction.
    MINTi MINT-1849631.
    STRINGi 10090.ENSMUSP00000023088.

    Protein family/group databases

    CAZyi GH27. Glycoside Hydrolase Family 27.

    PTM databases

    PhosphoSitei Q9QWR8.

    Proteomic databases

    MaxQBi Q9QWR8.
    PaxDbi Q9QWR8.
    PRIDEi Q9QWR8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023088 ; ENSMUSP00000023088 ; ENSMUSG00000022453 .
    GeneIDi 17939.
    KEGGi mmu:17939.
    UCSCi uc007wyw.2. mouse.

    Organism-specific databases

    CTDi 4668.
    MGIi MGI:1261422. Naga.

    Phylogenomic databases

    eggNOGi NOG68897.
    GeneTreei ENSGT00390000008751.
    HOGENOMi HOG000161224.
    HOVERGENi HBG001989.
    InParanoidi Q9QWR8.
    KOi K01204.
    OMAi TNFTVVI.
    OrthoDBi EOG7F24SV.
    PhylomeDBi Q9QWR8.
    TreeFami TF312909.

    Miscellaneous databases

    NextBioi 292845.
    PROi Q9QWR8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9QWR8.
    CleanExi MM_NAGA.
    Genevestigatori Q9QWR8.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02065. Melibiase. 1 hit.
    [Graphical view ]
    PRINTSi PR00740. GLHYDRLASE27.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Murine alpha-N-acetylgalactosaminidase: isolation and expression of a full-length cDNA and genomic organization: further evidence of an alpha-galactosidase gene family."
      Wang A.M., Ioannou Y.A., Zeidner K.M., Desnick R.J.
      Mol. Genet. Metab. 65:165-173(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular cloning, structural organization, sequence, chromosomal assignment, and expression of the mouse alpha-N-acetylgalactosaminidase gene."
      Herrmann T., Schindler D., Tabe H., Onodera O., Igarashi S., Polack A., Zehnpfennig D., Tsuji S.
      Gene 211:205-214(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
      Tissue: Liver.
    3. "High-throughput sequence identification of gene coding variants within alcohol-related QTLs."
      Ehringer M.A., Thompson J., Conroy O., Xu Y., Yang F., Canniff J., Beeson M., Gordon L., Bennett B., Johnson T.E., Sikela J.M.
      Mamm. Genome 12:657-663(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ILS and ISS.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiNAGAB_MOUSE
    AccessioniPrimary (citable) accession number: Q9QWR8
    Secondary accession number(s): O88620, Q8R437, Q8VDK2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3