Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9QWH1 (PHC2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polyhomeotic-like protein 2

Short name=mPH2
Alternative name(s):
Early development regulatory protein 2
p36
Gene names
Name:Phc2
Synonyms:Edr2, Ph2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length850 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Ref.7

Subunit structure

Interacts with N-terminal region of the SP1 transcription factor and with MAPKAPK2. Component of a PRC1-like complex. Interacts with CBX4 By similarity. Interacts with BMI1, PCGF2, PHC1 and RNF2. Interacts with CHTOP. Ref.5 Ref.7 Ref.8

Subcellular location

Nucleus Probable.

Tissue specificity

Isoform 2 is ubiquitously expressed in embryos and adult tissues at much higher level than isoform 1. Ref.1

Developmental stage

Detected at E11.5 in the developing brain, in the ventricular zones of the cortex and ganglionic eminences as well as in adult, in mature structures such as the granule cell layer of the dentate gyrus and cerebellum. Ref.6

Domain

HD1 motif interacts with SAM domain of PHC1. Ref.1

Disruption phenotype

Mice are viable and fertile but show posterior transformations of the axial skeleton and premature senescence of mouse embryonic fibroblast associated with derepression of Hox cluster genes and Cdkn2a genes, respectively. Mice lacking Phc2 and Phc1 dies at an early gestational stage. Mice mutant for Phc1 and/or Phc2 demonstrate that Phc1 and Phc2 mutations affect synergistically the survival of embryos in a gene dosage-dependent manner and thus show functional redundancy of Phc1 and Phc2. Ref.7

Sequence similarities

Contains 1 FCS-type zinc finger.

Contains 1 SAM (sterile alpha motif) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9QWH1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9QWH1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-527: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 850850Polyhomeotic-like protein 2
PRO_0000076287

Regions

Domain786 – 85065SAM
Zinc finger625 – 65935FCS-type
Motif550 – 57930HD1
Compositional bias34 – 418Poly-Gly
Compositional bias58 – 11457Gln-rich

Amino acid modifications

Modified residue6131Phosphoserine By similarity

Natural variations

Alternative sequence1 – 527527Missing in isoform 2.
VSP_016919

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 87746AFD7808DC7B

FASTA85089,799
        10         20         30         40         50         60 
MENELPVPHT SNRASVTTNT SGTNSSSGCI SSSGGGGGSG GRPTAPQISV YSGIPDRQTV 

        70         80         90        100        110        120 
QVIQQALHRQ PSTAAQYLQQ MYAAQQQHLM LQTAALQQQH LSSAQLQSLA AVQQASLVAN 

       130        140        150        160        170        180 
RQGSTPGSSV SSQAPAQSSS LNLAASPAAA QLINRAQSVN SAAASGLAQQ AVLLGNTSSP 

       190        200        210        220        230        240 
ALTASQAQMY LRAQMLIFTP TATVATVQPE LCTGSPARPP TPAQVQNLTL RTQQTPAAAA 

       250        260        270        280        290        300 
SGPPPTQPVL PSLALKPTPS SSQPLPAPPQ GRTMAQGSPA GAKPSGTDNA PETLKAGDGN 

       310        320        330        340        350        360 
CNMEGRPGPG RAVPAVATHP LIAPAYAHLQ SHQLLPQPPA KHPQPQFVAQ QQPQPPRPAP 

       370        380        390        400        410        420 
QVQSQPQLAS VSPSLALQSS PEDHALPLGS VTQALPLQCS TTHVHKPGNS QQCHLPTLDT 

       430        440        450        460        470        480 
GSQNGHPEGG SHPPQRRFQH TSAVILQVQP ASPVTPQQCA PDDWKEVVPA EKSVPVARPG 

       490        500        510        520        530        540 
PSPHQQAIIP AIPGGLPGPK SPNIQQCPAH ETGQGIVHAL TDLSSPGMTS GNGNSASSIA 

       550        560        570        580        590        600 
GTAPQNGENK PPQAIVKPQI LTHVIEGFVI QEGAEPFPVG RSSLLVGNLK KKYAQGFLPE 

       610        620        630        640        650        660 
KPPQQDHTTT TDSEMEEPYL QESKEEGTPL KLKCELCGRV DFAYKFKRSK RFCSMACAKR 

       670        680        690        700        710        720 
YNVGCTKRVG LFHSDRSKLQ KAGTTTHNRR RASKASLPTL TKDTKKQPSG TVPLSVTAAL 

       730        740        750        760        770        780 
QLAHSQEDSS RCSDNSSYEE PLSPISASSS TSRRRQGQRD LDLPDMHMRD LVGVGHHFLP 

       790        800        810        820        830        840 
SEPTKWNVED VYEFIRSLPG CQEIAEEFRA QEIDGQALLL LKEDHLMSAM NIKLGPALKI 

       850 
YARISMLKDS 

« Hide

Isoform 2 [UniParc].

Checksum: ECEA73FEA72710E1
Show »

FASTA32335,748

References

« Hide 'large scale' references
[1]"The mouse Edr2 (Mph2) gene has two forms of mRNA encoding 90- and 36-kDa polypeptides."
Yamaki M., Isono K., Takada Y., Abe K., Akasaka T., Tanzawa H., Koseki H.
Gene 288:103-110(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), DOMAIN HD1 MOTIF, TISSUE SPECIFICITY.
[2]"The basic helix-loop-helix protein Th1 interacts with polycomb-group proteins."
Howard T.L., Ingermann A.R., Hollenberg S.M.
Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: NIH Swiss.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6 and FVB/N.
Tissue: Brain and Mammary tumor.
[5]"The Bmi-1 oncoprotein interacts with dinG and MPh2: the role of RING finger domains."
Hemenway C.S., Halligan B.W., Levy L.S.
Oncogene 16:2541-2547(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 419-850 (ISOFORM 1), INTERACTION WITH BMI1 AND RNF2.
[6]"Mph2 expression in germinal zones of the mouse brain."
Kim M.H., Gunnersen J.M., Tan S.S.
Dev. Dyn. 232:209-215(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[7]"Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes."
Isono K., Fujimura Y., Shinga J., Yamaki M., O-Wang J., Takihara Y., Murahashi Y., Takada Y., Mizutani-Koseki Y., Koseki H.
Mol. Cell. Biol. 25:6694-6706(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH PCGF2; PHC1 AND RNF2.
[8]"Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation."
Fanis P., Gillemans N., Aghajanirefah A., Pourfarzad F., Demmers J., Esteghamat F., Vadlamudi R.K., Grosveld F., Philipsen S., van Dijk T.B.
Mol. Cell. Proteomics 11:1263-1273(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CHTOP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB062362 mRNA. Translation: BAB93527.1.
U81491 mRNA. Translation: AAD00519.1.
AL611969, AL611983 Genomic DNA. Translation: CAM23052.1.
AL611983 Genomic DNA. Translation: CAM25248.1.
AL611983, AL611969 Genomic DNA. Translation: CAM25252.1.
BC057571 mRNA. Translation: AAH57571.1.
BC071246 mRNA. Translation: AAH71246.1.
AF060076 mRNA. Translation: AAC23570.1.
CCDSCCDS18674.1. [Q9QWH1-1]
CCDS57297.1. [Q9QWH1-2]
RefSeqNP_001182012.1. NM_001195083.1. [Q9QWH1-2]
NP_001182059.1. NM_001195130.1. [Q9QWH1-1]
NP_061244.1. NM_018774.4. [Q9QWH1-1]
XP_006503300.1. XM_006503237.1. [Q9QWH1-2]
XP_006503301.1. XM_006503238.1. [Q9QWH1-2]
UniGeneMm.473738.
Mm.490300.

3D structure databases

ProteinModelPortalQ9QWH1.
SMRQ9QWH1. Positions 632-662, 784-844.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207636. 9 interactions.
IntActQ9QWH1. 9 interactions.
MINTMINT-1728977.

PTM databases

PhosphoSiteQ9QWH1.

Proteomic databases

MaxQBQ9QWH1.
PaxDbQ9QWH1.
PRIDEQ9QWH1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030588; ENSMUSP00000030588; ENSMUSG00000028796. [Q9QWH1-1]
ENSMUST00000106079; ENSMUSP00000101689; ENSMUSG00000028796. [Q9QWH1-2]
ENSMUST00000106080; ENSMUSP00000101690; ENSMUSG00000028796. [Q9QWH1-1]
GeneID54383.
KEGGmmu:54383.
UCSCuc008uvh.2. mouse. [Q9QWH1-1]
uc008uvk.2. mouse. [Q9QWH1-2]

Organism-specific databases

CTD1912.
MGIMGI:1860454. Phc2.

Phylogenomic databases

eggNOGNOG145736.
GeneTreeENSGT00550000074459.
HOGENOMHOG000236275.
HOVERGENHBG039325.
InParanoidB1ASA4.
KOK11457.
OMAYPEGLSH.
OrthoDBEOG789C9Q.
PhylomeDBQ9QWH1.
TreeFamTF331299.

Gene expression databases

ArrayExpressQ9QWH1.
BgeeQ9QWH1.
CleanExMM_PHC2.
GenevestigatorQ9QWH1.

Family and domain databases

Gene3D1.10.150.50. 1 hit.
InterProIPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR012313. Znf_FCS.
[Graphical view]
PfamPF00536. SAM_1. 1 hit.
[Graphical view]
SMARTSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMSSF47769. SSF47769. 1 hit.
PROSITEPS50105. SAM_DOMAIN. 1 hit.
PS51024. ZF_FCS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPHC2. mouse.
NextBio311212.
PROQ9QWH1.
SOURCESearch...

Entry information

Entry namePHC2_MOUSE
AccessionPrimary (citable) accession number: Q9QWH1
Secondary accession number(s): B1AS98 expand/collapse secondary AC list , B1ASA4, O88463, Q8K5D9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot