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Protein

Polyhomeotic-like protein 2

Gene

Phc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri625 – 65935FCS-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. multicellular organismal development Source: UniProtKB-KW
  2. spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_188970. Oxidative Stress Induced Senescence.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyhomeotic-like protein 2
Short name:
mPH2
Alternative name(s):
Early development regulatory protein 2
p36
Gene namesi
Name:Phc2
Synonyms:Edr2, Ph2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1860454. Phc2.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. heterochromatin Source: MGI
  2. nucleoplasm Source: MGI
  3. nucleus Source: MGI
  4. PcG protein complex Source: MGI
  5. PRC1 complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile but show posterior transformations of the axial skeleton and premature senescence of mouse embryonic fibroblast associated with derepression of Hox cluster genes and Cdkn2a genes, respectively. Mice lacking Phc2 and Phc1 dies at an early gestational stage. Mice mutant for Phc1 and/or Phc2 demonstrate that Phc1 and Phc2 mutations affect synergistically the survival of embryos in a gene dosage-dependent manner and thus show functional redundancy of Phc1 and Phc2.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 850850Polyhomeotic-like protein 2PRO_0000076287Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei613 – 6131PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9QWH1.
PaxDbiQ9QWH1.
PRIDEiQ9QWH1.

PTM databases

PhosphoSiteiQ9QWH1.

Expressioni

Tissue specificityi

Isoform 2 is ubiquitously expressed in embryos and adult tissues at much higher level than isoform 1.1 Publication

Developmental stagei

Detected at E11.5 in the developing brain, in the ventricular zones of the cortex and ganglionic eminences as well as in adult, in mature structures such as the granule cell layer of the dentate gyrus and cerebellum.1 Publication

Gene expression databases

BgeeiQ9QWH1.
CleanExiMM_PHC2.
ExpressionAtlasiQ9QWH1. baseline and differential.
GenevestigatoriQ9QWH1.

Interactioni

Subunit structurei

Interacts with N-terminal region of the SP1 transcription factor and with MAPKAPK2. Component of a PRC1-like complex. Interacts with CBX4 (By similarity). Interacts with BMI1, PCGF2, PHC1 and RNF2. Interacts with CHTOP.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Bmi1P259163EBI-642357,EBI-927401
MAPKAPK2P491372EBI-642357,EBI-993299From a different organism.
Mapkapk2P491385EBI-642357,EBI-298776
Pcgf2P237985EBI-642357,EBI-926857
Phc1Q640282EBI-642357,EBI-927346
Ring1O357302EBI-642357,EBI-929310
Rnf2Q9CQJ47EBI-642357,EBI-927321

Protein-protein interaction databases

BioGridi207636. 9 interactions.
IntActiQ9QWH1. 9 interactions.
MINTiMINT-1728977.

Structurei

3D structure databases

ProteinModelPortaliQ9QWH1.
SMRiQ9QWH1. Positions 632-662, 782-848.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini786 – 85065SAMPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi550 – 57930HD1Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi34 – 418Poly-Gly
Compositional biasi58 – 11457Gln-richAdd
BLAST

Domaini

HD1 motif interacts with SAM domain of PHC1.1 Publication

Sequence similaritiesi

Contains 1 FCS-type zinc finger.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri625 – 65935FCS-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG145736.
GeneTreeiENSGT00550000074459.
HOGENOMiHOG000236275.
HOVERGENiHBG039325.
InParanoidiQ9QWH1.
KOiK11457.
OMAiYPEGLSH.
OrthoDBiEOG789C9Q.
PhylomeDBiQ9QWH1.
TreeFamiTF331299.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR012313. Znf_FCS.
[Graphical view]
PfamiPF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
PS51024. ZF_FCS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QWH1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENELPVPHT SNRASVTTNT SGTNSSSGCI SSSGGGGGSG GRPTAPQISV
60 70 80 90 100
YSGIPDRQTV QVIQQALHRQ PSTAAQYLQQ MYAAQQQHLM LQTAALQQQH
110 120 130 140 150
LSSAQLQSLA AVQQASLVAN RQGSTPGSSV SSQAPAQSSS LNLAASPAAA
160 170 180 190 200
QLINRAQSVN SAAASGLAQQ AVLLGNTSSP ALTASQAQMY LRAQMLIFTP
210 220 230 240 250
TATVATVQPE LCTGSPARPP TPAQVQNLTL RTQQTPAAAA SGPPPTQPVL
260 270 280 290 300
PSLALKPTPS SSQPLPAPPQ GRTMAQGSPA GAKPSGTDNA PETLKAGDGN
310 320 330 340 350
CNMEGRPGPG RAVPAVATHP LIAPAYAHLQ SHQLLPQPPA KHPQPQFVAQ
360 370 380 390 400
QQPQPPRPAP QVQSQPQLAS VSPSLALQSS PEDHALPLGS VTQALPLQCS
410 420 430 440 450
TTHVHKPGNS QQCHLPTLDT GSQNGHPEGG SHPPQRRFQH TSAVILQVQP
460 470 480 490 500
ASPVTPQQCA PDDWKEVVPA EKSVPVARPG PSPHQQAIIP AIPGGLPGPK
510 520 530 540 550
SPNIQQCPAH ETGQGIVHAL TDLSSPGMTS GNGNSASSIA GTAPQNGENK
560 570 580 590 600
PPQAIVKPQI LTHVIEGFVI QEGAEPFPVG RSSLLVGNLK KKYAQGFLPE
610 620 630 640 650
KPPQQDHTTT TDSEMEEPYL QESKEEGTPL KLKCELCGRV DFAYKFKRSK
660 670 680 690 700
RFCSMACAKR YNVGCTKRVG LFHSDRSKLQ KAGTTTHNRR RASKASLPTL
710 720 730 740 750
TKDTKKQPSG TVPLSVTAAL QLAHSQEDSS RCSDNSSYEE PLSPISASSS
760 770 780 790 800
TSRRRQGQRD LDLPDMHMRD LVGVGHHFLP SEPTKWNVED VYEFIRSLPG
810 820 830 840 850
CQEIAEEFRA QEIDGQALLL LKEDHLMSAM NIKLGPALKI YARISMLKDS
Length:850
Mass (Da):89,799
Last modified:May 1, 2000 - v1
Checksum:i87746AFD7808DC7B
GO
Isoform 2 (identifier: Q9QWH1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-527: Missing.

Show »
Length:323
Mass (Da):35,748
Checksum:iECEA73FEA72710E1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 527527Missing in isoform 2. 2 PublicationsVSP_016919Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB062362 mRNA. Translation: BAB93527.1.
U81491 mRNA. Translation: AAD00519.1.
AL611969, AL611983 Genomic DNA. Translation: CAM23052.1.
AL611983 Genomic DNA. Translation: CAM25248.1.
AL611983, AL611969 Genomic DNA. Translation: CAM25252.1.
BC057571 mRNA. Translation: AAH57571.1.
BC071246 mRNA. Translation: AAH71246.1.
AF060076 mRNA. Translation: AAC23570.1.
CCDSiCCDS18674.1. [Q9QWH1-1]
CCDS57297.1. [Q9QWH1-2]
RefSeqiNP_001182012.1. NM_001195083.1. [Q9QWH1-2]
NP_001182059.1. NM_001195130.1. [Q9QWH1-1]
NP_061244.1. NM_018774.4. [Q9QWH1-1]
XP_006503300.1. XM_006503237.1. [Q9QWH1-2]
XP_006503301.1. XM_006503238.1. [Q9QWH1-2]
UniGeneiMm.473738.
Mm.490300.

Genome annotation databases

EnsembliENSMUST00000030588; ENSMUSP00000030588; ENSMUSG00000028796. [Q9QWH1-1]
ENSMUST00000106079; ENSMUSP00000101689; ENSMUSG00000028796. [Q9QWH1-2]
ENSMUST00000106080; ENSMUSP00000101690; ENSMUSG00000028796. [Q9QWH1-1]
GeneIDi54383.
KEGGimmu:54383.
UCSCiuc008uvh.2. mouse. [Q9QWH1-1]
uc008uvk.2. mouse. [Q9QWH1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB062362 mRNA. Translation: BAB93527.1.
U81491 mRNA. Translation: AAD00519.1.
AL611969, AL611983 Genomic DNA. Translation: CAM23052.1.
AL611983 Genomic DNA. Translation: CAM25248.1.
AL611983, AL611969 Genomic DNA. Translation: CAM25252.1.
BC057571 mRNA. Translation: AAH57571.1.
BC071246 mRNA. Translation: AAH71246.1.
AF060076 mRNA. Translation: AAC23570.1.
CCDSiCCDS18674.1. [Q9QWH1-1]
CCDS57297.1. [Q9QWH1-2]
RefSeqiNP_001182012.1. NM_001195083.1. [Q9QWH1-2]
NP_001182059.1. NM_001195130.1. [Q9QWH1-1]
NP_061244.1. NM_018774.4. [Q9QWH1-1]
XP_006503300.1. XM_006503237.1. [Q9QWH1-2]
XP_006503301.1. XM_006503238.1. [Q9QWH1-2]
UniGeneiMm.473738.
Mm.490300.

3D structure databases

ProteinModelPortaliQ9QWH1.
SMRiQ9QWH1. Positions 632-662, 782-848.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207636. 9 interactions.
IntActiQ9QWH1. 9 interactions.
MINTiMINT-1728977.

PTM databases

PhosphoSiteiQ9QWH1.

Proteomic databases

MaxQBiQ9QWH1.
PaxDbiQ9QWH1.
PRIDEiQ9QWH1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030588; ENSMUSP00000030588; ENSMUSG00000028796. [Q9QWH1-1]
ENSMUST00000106079; ENSMUSP00000101689; ENSMUSG00000028796. [Q9QWH1-2]
ENSMUST00000106080; ENSMUSP00000101690; ENSMUSG00000028796. [Q9QWH1-1]
GeneIDi54383.
KEGGimmu:54383.
UCSCiuc008uvh.2. mouse. [Q9QWH1-1]
uc008uvk.2. mouse. [Q9QWH1-2]

Organism-specific databases

CTDi1912.
MGIiMGI:1860454. Phc2.

Phylogenomic databases

eggNOGiNOG145736.
GeneTreeiENSGT00550000074459.
HOGENOMiHOG000236275.
HOVERGENiHBG039325.
InParanoidiQ9QWH1.
KOiK11457.
OMAiYPEGLSH.
OrthoDBiEOG789C9Q.
PhylomeDBiQ9QWH1.
TreeFamiTF331299.

Enzyme and pathway databases

ReactomeiREACT_188970. Oxidative Stress Induced Senescence.

Miscellaneous databases

ChiTaRSiPhc2. mouse.
NextBioi311212.
PROiQ9QWH1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QWH1.
CleanExiMM_PHC2.
ExpressionAtlasiQ9QWH1. baseline and differential.
GenevestigatoriQ9QWH1.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR021129. SAM_type1.
IPR012313. Znf_FCS.
[Graphical view]
PfamiPF00536. SAM_1. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
PS51024. ZF_FCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse Edr2 (Mph2) gene has two forms of mRNA encoding 90- and 36-kDa polypeptides."
    Yamaki M., Isono K., Takada Y., Abe K., Akasaka T., Tanzawa H., Koseki H.
    Gene 288:103-110(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), DOMAIN HD1 MOTIF, TISSUE SPECIFICITY.
  2. "The basic helix-loop-helix protein Th1 interacts with polycomb-group proteins."
    Howard T.L., Ingermann A.R., Hollenberg S.M.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: NIH Swiss.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6 and FVB/N.
    Tissue: Brain and Mammary tumor.
  5. "The Bmi-1 oncoprotein interacts with dinG and MPh2: the role of RING finger domains."
    Hemenway C.S., Halligan B.W., Levy L.S.
    Oncogene 16:2541-2547(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 419-850 (ISOFORM 1), INTERACTION WITH BMI1 AND RNF2.
  6. "Mph2 expression in germinal zones of the mouse brain."
    Kim M.H., Gunnersen J.M., Tan S.S.
    Dev. Dyn. 232:209-215(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  7. "Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes."
    Isono K., Fujimura Y., Shinga J., Yamaki M., O-Wang J., Takihara Y., Murahashi Y., Takada Y., Mizutani-Koseki Y., Koseki H.
    Mol. Cell. Biol. 25:6694-6706(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH PCGF2; PHC1 AND RNF2.
  8. "Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation."
    Fanis P., Gillemans N., Aghajanirefah A., Pourfarzad F., Demmers J., Esteghamat F., Vadlamudi R.K., Grosveld F., Philipsen S., van Dijk T.B.
    Mol. Cell. Proteomics 11:1263-1273(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CHTOP.

Entry informationi

Entry nameiPHC2_MOUSE
AccessioniPrimary (citable) accession number: Q9QWH1
Secondary accession number(s): B1AS98
, B1ASA4, O88463, Q8K5D9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: May 1, 2000
Last modified: March 4, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.