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Protein

Polyhomeotic-like protein 2

Gene

Phc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri625 – 659FCS-typePROSITE-ProRule annotationAdd BLAST35

GO - Molecular functioni

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • spermatogenesis Source: MGI

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-2559580 Oxidative Stress Induced Senescence
R-MMU-3108214 SUMOylation of DNA damage response and repair proteins
R-MMU-4551638 SUMOylation of chromatin organization proteins
R-MMU-4570464 SUMOylation of RNA binding proteins
R-MMU-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known
R-MMU-8943724 Regulation of PTEN gene transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Polyhomeotic-like protein 2
Short name:
mPH2
Alternative name(s):
Early development regulatory protein 2
p36
Gene namesi
Name:Phc2
Synonyms:Edr2, Ph2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1860454 Phc2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile but show posterior transformations of the axial skeleton and premature senescence of mouse embryonic fibroblast associated with derepression of Hox cluster genes and Cdkn2a genes, respectively. Mice lacking Phc2 and Phc1 die at an early gestational stage. Mice mutant for Phc1 and/or Phc2 demonstrate that Phc1 and Phc2 mutations affect synergistically the survival of embryos in a gene dosage-dependent manner and thus show functional redundancy of Phc1 and Phc2.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762871 – 850Polyhomeotic-like protein 2Add BLAST850

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki590Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki592Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei611PhosphothreonineBy similarity1
Modified residuei613PhosphoserineBy similarity1
Cross-linki624Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki694Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei743PhosphoserineBy similarity1
Cross-linki839Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9QWH1
PeptideAtlasiQ9QWH1
PRIDEiQ9QWH1

PTM databases

iPTMnetiQ9QWH1
PhosphoSitePlusiQ9QWH1

Expressioni

Tissue specificityi

Isoform 2 is ubiquitously expressed in embryos and adult tissues at much higher level than isoform 1.1 Publication

Developmental stagei

Detected at E11.5 in the developing brain, in the ventricular zones of the cortex and ganglionic eminences as well as in adult, in mature structures such as the granule cell layer of the dentate gyrus and cerebellum.1 Publication

Gene expression databases

BgeeiENSMUSG00000028796
CleanExiMM_PHC2
ExpressionAtlasiQ9QWH1 baseline and differential
GenevisibleiQ9QWH1 MM

Interactioni

Subunit structurei

Component of a PRC1-like complex. Interacts with CBX4 (By similarity). Interacts with BMI1, PCGF2, PHC1 and RNF2 (PubMed:9627119, PubMed:16024804, PubMed:27827373). Interacts with CHTOP (PubMed:22872859). Interacts with the N-terminal region of the SP1 transcription factor and with MAPKAPK2 (By similarity).By similarity4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207636, 9 interactors
IntActiQ9QWH1, 9 interactors
MINTiQ9QWH1
STRINGi10090.ENSMUSP00000030588

Structurei

Secondary structure

1850
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi560 – 565Combined sources6
Beta strandi568 – 573Combined sources6
Turni578 – 580Combined sources3
Helixi583 – 586Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NA1NMR-A557-590[»]
ProteinModelPortaliQ9QWH1
SMRiQ9QWH1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini786 – 850SAMPROSITE-ProRule annotationAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 79Intrinsically disordered1 PublicationAdd BLAST79
Regioni33 – 56Interaction with BMI11 PublicationAdd BLAST24

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi550 – 579HD1Add BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi34 – 41Poly-Gly8
Compositional biasi58 – 114Gln-richAdd BLAST57

Domaini

HD1 motif interacts with SAM domain of PHC1.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri625 – 659FCS-typePROSITE-ProRule annotationAdd BLAST35

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IDZN Eukaryota
ENOG4111F0Y LUCA
GeneTreeiENSGT00550000074459
HOGENOMiHOG000236275
HOVERGENiHBG039325
InParanoidiQ9QWH1
KOiK11457
OMAiNELPVPH
OrthoDBiEOG091G02VS
PhylomeDBiQ9QWH1
TreeFamiTF331299

Family and domain databases

Gene3Di3.30.60.160, 1 hit
InterProiView protein in InterPro
IPR001660 SAM
IPR013761 SAM/pointed_sf
IPR012313 Znf_FCS
IPR038603 Znf_FCS_sf
PfamiView protein in Pfam
PF00536 SAM_1, 1 hit
SMARTiView protein in SMART
SM00454 SAM, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
PROSITEiView protein in PROSITE
PS50105 SAM_DOMAIN, 1 hit
PS51024 ZF_FCS, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QWH1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENELPVPHT SNRASVTTNT SGTNSSSGCI SSSGGGGGSG GRPTAPQISV
60 70 80 90 100
YSGIPDRQTV QVIQQALHRQ PSTAAQYLQQ MYAAQQQHLM LQTAALQQQH
110 120 130 140 150
LSSAQLQSLA AVQQASLVAN RQGSTPGSSV SSQAPAQSSS LNLAASPAAA
160 170 180 190 200
QLINRAQSVN SAAASGLAQQ AVLLGNTSSP ALTASQAQMY LRAQMLIFTP
210 220 230 240 250
TATVATVQPE LCTGSPARPP TPAQVQNLTL RTQQTPAAAA SGPPPTQPVL
260 270 280 290 300
PSLALKPTPS SSQPLPAPPQ GRTMAQGSPA GAKPSGTDNA PETLKAGDGN
310 320 330 340 350
CNMEGRPGPG RAVPAVATHP LIAPAYAHLQ SHQLLPQPPA KHPQPQFVAQ
360 370 380 390 400
QQPQPPRPAP QVQSQPQLAS VSPSLALQSS PEDHALPLGS VTQALPLQCS
410 420 430 440 450
TTHVHKPGNS QQCHLPTLDT GSQNGHPEGG SHPPQRRFQH TSAVILQVQP
460 470 480 490 500
ASPVTPQQCA PDDWKEVVPA EKSVPVARPG PSPHQQAIIP AIPGGLPGPK
510 520 530 540 550
SPNIQQCPAH ETGQGIVHAL TDLSSPGMTS GNGNSASSIA GTAPQNGENK
560 570 580 590 600
PPQAIVKPQI LTHVIEGFVI QEGAEPFPVG RSSLLVGNLK KKYAQGFLPE
610 620 630 640 650
KPPQQDHTTT TDSEMEEPYL QESKEEGTPL KLKCELCGRV DFAYKFKRSK
660 670 680 690 700
RFCSMACAKR YNVGCTKRVG LFHSDRSKLQ KAGTTTHNRR RASKASLPTL
710 720 730 740 750
TKDTKKQPSG TVPLSVTAAL QLAHSQEDSS RCSDNSSYEE PLSPISASSS
760 770 780 790 800
TSRRRQGQRD LDLPDMHMRD LVGVGHHFLP SEPTKWNVED VYEFIRSLPG
810 820 830 840 850
CQEIAEEFRA QEIDGQALLL LKEDHLMSAM NIKLGPALKI YARISMLKDS
Length:850
Mass (Da):89,799
Last modified:May 1, 2000 - v1
Checksum:i87746AFD7808DC7B
GO
Isoform 2 (identifier: Q9QWH1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-527: Missing.

Show »
Length:323
Mass (Da):35,748
Checksum:iECEA73FEA72710E1
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0169191 – 527Missing in isoform 2. 2 PublicationsAdd BLAST527

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB062362 mRNA Translation: BAB93527.1
U81491 mRNA Translation: AAD00519.1
AL611969, AL611983 Genomic DNA Translation: CAM23052.1
AL611983 Genomic DNA Translation: CAM25248.1
AL611983, AL611969 Genomic DNA Translation: CAM25252.1
BC057571 mRNA Translation: AAH57571.1
BC071246 mRNA Translation: AAH71246.1
AF060076 mRNA Translation: AAC23570.1
CCDSiCCDS18674.1 [Q9QWH1-1]
CCDS57297.1 [Q9QWH1-2]
RefSeqiNP_001182012.1, NM_001195083.1 [Q9QWH1-2]
NP_001182059.1, NM_001195130.1 [Q9QWH1-1]
NP_061244.1, NM_018774.4 [Q9QWH1-1]
XP_006503300.1, XM_006503237.3 [Q9QWH1-2]
XP_006503301.1, XM_006503238.1 [Q9QWH1-2]
XP_017175809.1, XM_017320320.1 [Q9QWH1-1]
UniGeneiMm.473738
Mm.490300

Genome annotation databases

EnsembliENSMUST00000030588; ENSMUSP00000030588; ENSMUSG00000028796 [Q9QWH1-1]
ENSMUST00000106079; ENSMUSP00000101689; ENSMUSG00000028796 [Q9QWH1-2]
ENSMUST00000106080; ENSMUSP00000101690; ENSMUSG00000028796 [Q9QWH1-1]
GeneIDi54383
KEGGimmu:54383
UCSCiuc008uvh.2 mouse [Q9QWH1-1]
uc008uvk.2 mouse [Q9QWH1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPHC2_MOUSE
AccessioniPrimary (citable) accession number: Q9QWH1
Secondary accession number(s): B1AS98
, B1ASA4, O88463, Q8K5D9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health