Reviewed, UniProtKB/Swiss-Prot Q9QWG5 (INP4B_RAT)

Names and origin

Protein names

Recommended name:
    Type II inositol-3,4-bisphosphate 4-phosphatase
    EC=3.1.3.66
Alternative name(s):
    Inositol polyphosphate 4-phosphatase type II

Gene names

Name:

Inpp4b

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	928 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate.
Catalytic activity	1-phosphatidyl-myo-inositol 3,4-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate.
Enzyme regulation	Strongly inhibited by inositol hexakisphosphate. Ref.1
Pathway	Signal transduction; phosphatidylinositol signaling pathway.
Sequence similarities	Belongs to the inositol-3,4-bisphosphate 4-phosphatase family. Contains 1 C2 domain.
biophysicochemical properties	Kinetic parameters: KM=39 µM for inositol 3,4-bisphosphate KM=34 µM for inositol 1,3,4-trisphosphate Vmax=26 µmol/min/mg enzyme with inositol 3,4-bisphosphate as substrate Vmax=22 µmol/min/mg enzyme with inositol 1,3,4-trisphosphate as substrate pH dependence: Optimum pH is 7-8.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase
Gene Ontology (GO)
Molecular function	phosphatidyl-inositol-4,5-bisphosphate 4-phosphatase activity Inferred from electronic annotation. Source: EC phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9QWG5-1)

Also known as: Alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9QWG5-2)

Also known as: Beta;

The sequence of this isoform differs from the canonical sequence as follows:
     885-928: REGCRIENVL...PEGTYGKADT → SRQTQGALNE...SLALLLAKYQ

Note: Inactive.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 928	928	Type II inositol-3,4-bisphosphate 4-phosphatase		PRO_0000190239
Regions
	Domain	40 – 164	125	C2
Natural variations
	Alternative sequence	885 – 928	44	REGCR…GKADT → SRQTQGALNESDDPETGCLS DNKPTSRHFYPVALLLVSSH LLVVWLILSLALLLAKYQ in isoform 2.		VSP_015249

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Alpha) [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 973EB2EEA58E6430	FASTA	928	105,245
	10 20 30 40 50 60 MEIKEEGTSE EGQHFLPAAQ ANDPEDIQFT SIQKSPNEPQ LEFILACKDL VAPVSDRKLN 70 80 90 100 110 120 TVVQVSVIHP VEQTLTRYSS TEIVEGTKDP LFLTGVTFPS DYPIYEETRI KLTVYDVKDK 130 140 150 160 170 180 PHDTIRTSVL PEHKDPPPEV ARSFLGCASF KVGELLKSKE QLLSLSLRTS DGGKVVGTIE 190 200 210 220 230 240 VSLVKMGEIE DGDTDHITTD VQGQKCALVY DSTAPESLSG KENLPFMNAV LRNPVCKLYR 250 260 270 280 290 300 FPTSDNKWMR IREQMSESIL SFHIPKELIS LHIKEDLCRN QELKELGDLS PHWDNLRNNV 310 320 330 340 350 360 LSHCDQMVTM YQDILTELSK ETGSSFKSSS SKGEKTLEFV PINLHLQRMQ VHSPHLKDAL 370 380 390 400 410 420 YDVITVGAPA AHFQGFKNGG LRKLLHRFET ERRNTGYQFI YYSPENTAKA KEVLSSINQL 430 440 450 460 470 480 QPLVATHADL LLTSASQHSP DSLRSSLKLL SEKTELFVHA FKDQLVRSAL LALYTARPGG 490 500 510 520 530 540 ILRKPPSPKV STEEKSSQHD SPQQLRRQDS IPHHSDYDEE EWDRVWANVG KSLNCIIAKV 550 560 570 580 590 600 DKLIERDSRN DKSTGGDSSK DGDADPNLED SLTSHPREDW YEQLHPLILT LKECMAEVVN 610 620 630 640 650 660 RAKQSLTFVL LQELAYSLPQ CLMLTLRRDI VFSQALAGLV CGFIIKLHTS LHDPGFLQQL 670 680 690 700 710 720 HTVGLIVQYE GLLSTYSDEI GMLEDMAVGI SDLRKVAFKI TEATSNDVLP VLTGRREHYV 730 740 750 760 770 780 VEVKLPATVF ESLPLQIKEG QLLHVYPVLF NVGINEQQTL AERFGDVSLQ ESINQENFEL 790 800 810 820 830 840 VQEYYSIFME KMPPDYISHF QEQNDLKGLL DNLHQNIQAK KRKNVEIMWL AATICRKLNG 850 860 870 880 890 900 IRFTCCKSAK DRTSMSVTLE QCSILRDEHQ LHKDFFIRAL DCMRREGCRI ENVLKNIKCR 910 920 RYAFNMLQLM AFPKCYRPPE GTYGKADT
	Isoform 2 (Beta). Checksum: 65B9CB28D3AAC6E3	FASTA	942	106,485
	10 20 30 40 50 60 MEIKEEGTSE EGQHFLPAAQ ANDPEDIQFT SIQKSPNEPQ LEFILACKDL VAPVSDRKLN 70 80 90 100 110 120 TVVQVSVIHP VEQTLTRYSS TEIVEGTKDP LFLTGVTFPS DYPIYEETRI KLTVYDVKDK 130 140 150 160 170 180 PHDTIRTSVL PEHKDPPPEV ARSFLGCASF KVGELLKSKE QLLSLSLRTS DGGKVVGTIE 190 200 210 220 230 240 VSLVKMGEIE DGDTDHITTD VQGQKCALVY DSTAPESLSG KENLPFMNAV LRNPVCKLYR 250 260 270 280 290 300 FPTSDNKWMR IREQMSESIL SFHIPKELIS LHIKEDLCRN QELKELGDLS PHWDNLRNNV 310 320 330 340 350 360 LSHCDQMVTM YQDILTELSK ETGSSFKSSS SKGEKTLEFV PINLHLQRMQ VHSPHLKDAL 370 380 390 400 410 420 YDVITVGAPA AHFQGFKNGG LRKLLHRFET ERRNTGYQFI YYSPENTAKA KEVLSSINQL 430 440 450 460 470 480 QPLVATHADL LLTSASQHSP DSLRSSLKLL SEKTELFVHA FKDQLVRSAL LALYTARPGG 490 500 510 520 530 540 ILRKPPSPKV STEEKSSQHD SPQQLRRQDS IPHHSDYDEE EWDRVWANVG KSLNCIIAKV 550 560 570 580 590 600 DKLIERDSRN DKSTGGDSSK DGDADPNLED SLTSHPREDW YEQLHPLILT LKECMAEVVN 610 620 630 640 650 660 RAKQSLTFVL LQELAYSLPQ CLMLTLRRDI VFSQALAGLV CGFIIKLHTS LHDPGFLQQL 670 680 690 700 710 720 HTVGLIVQYE GLLSTYSDEI GMLEDMAVGI SDLRKVAFKI TEATSNDVLP VLTGRREHYV 730 740 750 760 770 780 VEVKLPATVF ESLPLQIKEG QLLHVYPVLF NVGINEQQTL AERFGDVSLQ ESINQENFEL 790 800 810 820 830 840 VQEYYSIFME KMPPDYISHF QEQNDLKGLL DNLHQNIQAK KRKNVEIMWL AATICRKLNG 850 860 870 880 890 900 IRFTCCKSAK DRTSMSVTLE QCSILRDEHQ LHKDFFIRAL DCMRSRQTQG ALNESDDPET 910 920 930 940 GCLSDNKPTS RHFYPVALLL VSSHLLVVWL ILSLALLLAK YQ

References

[1]

"The cDNA cloning and characterization of inositol polyphosphate 4-phosphatase type II. Evidence for conserved alternative splicing in the 4-phosphatase family." Norris F.A., Atkins R.C., Majerus P.W. J. Biol. Chem. 272:23859-23864(1997) [PubMed: 9295334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U96920 mRNA. Translation: AAB72151.1. U96921 mRNA. Translation: AAB72152.1.
IPI	IPI00212160. IPI00213063.
RefSeq	NP_446369.1.
UniGene	Rn.10829
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q9QWG5.
Genome annotation databases
Ensembl	ENSRNOG00000018382. Rattus norvegicus. [Contig view]
GeneID	116699.
KEGG	rno:116699.
Organism-specific databases
RGD	620470. Inpp4b.
Phylogenomic databases
HOVERGEN	Q9QWG5.
OMA	Q9QWG5. RMQVHSP.
Enzyme and pathway databases
BRENDA	3.1.3.66. 248.
Gene expression databases
ArrayExpress	Q9QWG5.
GermOnline	ENSRNOG00000018382. Rattus norvegicus.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. [Graphical view]
SMART	SM00239. C2. 1 hit. [Graphical view]
PROSITE	PS50004. C2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	619604.

Entry information

Entry name

INP4B_RAT

Accession

Primary (citable) accession number: Q9QWG5
Secondary accession number(s): O35825

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)