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Protein

Type II inositol 3,4-bisphosphate 4-phosphatase

Gene

Inpp4b

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 1,4-bisphosphate.

Catalytic activityi

1-phosphatidyl-myo-inositol 3,4-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate.

Enzyme regulationi

Strongly inhibited by inositol hexakisphosphate.1 Publication

Kineticsi

  1. KM=39 µM for inositol 3,4-bisphosphate1 Publication
  2. KM=34 µM for inositol 1,3,4-trisphosphate1 Publication
  1. Vmax=26 µmol/min/mg enzyme with inositol 3,4-bisphosphate as substrate1 Publication
  2. Vmax=22 µmol/min/mg enzyme with inositol 1,3,4-trisphosphate as substrate1 Publication

pH dependencei

Optimum pH is 7-8.1 Publication

Pathwayi: phosphatidylinositol signaling pathway

This protein is involved in the pathway phosphatidylinositol signaling pathway, which is part of Signal transduction.
View all proteins of this organism that are known to be involved in the pathway phosphatidylinositol signaling pathway and in Signal transduction.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.1.3.66. 5301.
ReactomeiR-RNO-1660499. Synthesis of PIPs at the plasma membrane.
R-RNO-1660516. Synthesis of PIPs at the early endosome membrane.
R-RNO-1855183. Synthesis of IP2, IP, and Ins in the cytosol.
UniPathwayiUPA00944.

Chemistry

SwissLipidsiSLP:000000896.

Names & Taxonomyi

Protein namesi
Recommended name:
Type II inositol 3,4-bisphosphate 4-phosphatase (EC:3.1.3.66)
Alternative name(s):
Inositol polyphosphate 4-phosphatase type II
Gene namesi
Name:Inpp4b
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi620470. Inpp4b.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 928928Type II inositol 3,4-bisphosphate 4-phosphatasePRO_0000190239Add
BLAST

Proteomic databases

PaxDbiQ9QWG5.
PRIDEiQ9QWG5.

PTM databases

iPTMnetiQ9QWG5.
PhosphoSiteiQ9QWG5.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018382.
GenevisibleiQ9QWG5. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025013.

Structurei

3D structure databases

ProteinModelPortaliQ9QWG5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 164125C2Add
BLAST

Sequence similaritiesi

Contains 1 C2 domain.Curated

Phylogenomic databases

eggNOGiKOG4428. Eukaryota.
ENOG410YV83. LUCA.
GeneTreeiENSGT00390000002033.
HOGENOMiHOG000113075.
HOVERGENiHBG081796.
InParanoidiQ9QWG5.
KOiK01109.
OMAiEFSLACK.
OrthoDBiEOG091G02IJ.
PhylomeDBiQ9QWG5.
TreeFamiTF325637.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QWG5-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEIKEEGTSE EGQHFLPAAQ ANDPEDIQFT SIQKSPNEPQ LEFILACKDL
60 70 80 90 100
VAPVSDRKLN TVVQVSVIHP VEQTLTRYSS TEIVEGTKDP LFLTGVTFPS
110 120 130 140 150
DYPIYEETRI KLTVYDVKDK PHDTIRTSVL PEHKDPPPEV ARSFLGCASF
160 170 180 190 200
KVGELLKSKE QLLSLSLRTS DGGKVVGTIE VSLVKMGEIE DGDTDHITTD
210 220 230 240 250
VQGQKCALVY DSTAPESLSG KENLPFMNAV LRNPVCKLYR FPTSDNKWMR
260 270 280 290 300
IREQMSESIL SFHIPKELIS LHIKEDLCRN QELKELGDLS PHWDNLRNNV
310 320 330 340 350
LSHCDQMVTM YQDILTELSK ETGSSFKSSS SKGEKTLEFV PINLHLQRMQ
360 370 380 390 400
VHSPHLKDAL YDVITVGAPA AHFQGFKNGG LRKLLHRFET ERRNTGYQFI
410 420 430 440 450
YYSPENTAKA KEVLSSINQL QPLVATHADL LLTSASQHSP DSLRSSLKLL
460 470 480 490 500
SEKTELFVHA FKDQLVRSAL LALYTARPGG ILRKPPSPKV STEEKSSQHD
510 520 530 540 550
SPQQLRRQDS IPHHSDYDEE EWDRVWANVG KSLNCIIAKV DKLIERDSRN
560 570 580 590 600
DKSTGGDSSK DGDADPNLED SLTSHPREDW YEQLHPLILT LKECMAEVVN
610 620 630 640 650
RAKQSLTFVL LQELAYSLPQ CLMLTLRRDI VFSQALAGLV CGFIIKLHTS
660 670 680 690 700
LHDPGFLQQL HTVGLIVQYE GLLSTYSDEI GMLEDMAVGI SDLRKVAFKI
710 720 730 740 750
TEATSNDVLP VLTGRREHYV VEVKLPATVF ESLPLQIKEG QLLHVYPVLF
760 770 780 790 800
NVGINEQQTL AERFGDVSLQ ESINQENFEL VQEYYSIFME KMPPDYISHF
810 820 830 840 850
QEQNDLKGLL DNLHQNIQAK KRKNVEIMWL AATICRKLNG IRFTCCKSAK
860 870 880 890 900
DRTSMSVTLE QCSILRDEHQ LHKDFFIRAL DCMRREGCRI ENVLKNIKCR
910 920
RYAFNMLQLM AFPKCYRPPE GTYGKADT
Length:928
Mass (Da):105,245
Last modified:May 1, 2000 - v1
Checksum:i973EB2EEA58E6430
GO
Isoform 2 (identifier: Q9QWG5-2) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     885-928: REGCRIENVL...PEGTYGKADT → SRQTQGALNE...SLALLLAKYQ

Note: Inactive.
Show »
Length:942
Mass (Da):106,485
Checksum:i65B9CB28D3AAC6E3
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei885 – 92844REGCR…GKADT → SRQTQGALNESDDPETGCLS DNKPTSRHFYPVALLLVSSH LLVVWLILSLALLLAKYQ in isoform 2. 1 PublicationVSP_015249Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96920 mRNA. Translation: AAB72151.1.
U96921 mRNA. Translation: AAB72152.1.
RefSeqiNP_446369.1. NM_053917.1. [Q9QWG5-2]
XP_008770684.1. XM_008772462.1. [Q9QWG5-2]
XP_008770685.1. XM_008772463.1. [Q9QWG5-1]
XP_008770686.1. XM_008772464.1. [Q9QWG5-1]
UniGeneiRn.10829.

Genome annotation databases

EnsembliENSRNOT00000024981; ENSRNOP00000024981; ENSRNOG00000018382. [Q9QWG5-1]
ENSRNOT00000025013; ENSRNOP00000025013; ENSRNOG00000018382. [Q9QWG5-2]
GeneIDi116699.
KEGGirno:116699.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96920 mRNA. Translation: AAB72151.1.
U96921 mRNA. Translation: AAB72152.1.
RefSeqiNP_446369.1. NM_053917.1. [Q9QWG5-2]
XP_008770684.1. XM_008772462.1. [Q9QWG5-2]
XP_008770685.1. XM_008772463.1. [Q9QWG5-1]
XP_008770686.1. XM_008772464.1. [Q9QWG5-1]
UniGeneiRn.10829.

3D structure databases

ProteinModelPortaliQ9QWG5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000025013.

Chemistry

SwissLipidsiSLP:000000896.

PTM databases

iPTMnetiQ9QWG5.
PhosphoSiteiQ9QWG5.

Proteomic databases

PaxDbiQ9QWG5.
PRIDEiQ9QWG5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024981; ENSRNOP00000024981; ENSRNOG00000018382. [Q9QWG5-1]
ENSRNOT00000025013; ENSRNOP00000025013; ENSRNOG00000018382. [Q9QWG5-2]
GeneIDi116699.
KEGGirno:116699.

Organism-specific databases

CTDi8821.
RGDi620470. Inpp4b.

Phylogenomic databases

eggNOGiKOG4428. Eukaryota.
ENOG410YV83. LUCA.
GeneTreeiENSGT00390000002033.
HOGENOMiHOG000113075.
HOVERGENiHBG081796.
InParanoidiQ9QWG5.
KOiK01109.
OMAiEFSLACK.
OrthoDBiEOG091G02IJ.
PhylomeDBiQ9QWG5.
TreeFamiTF325637.

Enzyme and pathway databases

UniPathwayiUPA00944.
BRENDAi3.1.3.66. 5301.
ReactomeiR-RNO-1660499. Synthesis of PIPs at the plasma membrane.
R-RNO-1660516. Synthesis of PIPs at the early endosome membrane.
R-RNO-1855183. Synthesis of IP2, IP, and Ins in the cytosol.

Miscellaneous databases

PROiQ9QWG5.

Gene expression databases

BgeeiENSRNOG00000018382.
GenevisibleiQ9QWG5. RN.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiINP4B_RAT
AccessioniPrimary (citable) accession number: Q9QWG5
Secondary accession number(s): O35825
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.