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Protein

GTP-binding protein SAR1b

Gene

SAR1B

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Activated by the guanine nucleotide exchange factor PREB. Involved in the selection of the protein cargo and the assembly of the COPII coat complex.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Magnesium1
Metal bindingi75Magnesium1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi32 – 39GTPBy similarity8
Nucleotide bindingi75 – 78GTPBy similarity4
Nucleotide bindingi134 – 137GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein SAR1b
Short name:
Sar1
Gene namesi
Name:SAR1B
Synonyms:SAR1, SARA2
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5F → D: Decreases recruitment to the endoplasmic reticulum. 1 Publication1
Mutagenesisi15 – 19VLQFL → AAAA: Blocks export of proteins from the ER to the Golgi. No effect on nucleotide binding. Abolishes localization to the endoplasmic reticulum. 1 Publication5
Mutagenesisi39T → N: Lowers affinity for GTP, but not for GDP. Inhibits export of proteins from the ER to the Golgi. 1 Publication1
Mutagenesisi158T → A: Blocks export of proteins from the ER to the Golgi. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002062601 – 198GTP-binding protein SAR1bAdd BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei164PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer. Binds PREB. Part of the COPII coat complex. Binds to the cytoplasmic tails of target proteins in the endoplasmic reticulum.1 Publication

Structurei

Secondary structure

1198
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 18Combined sources5
Beta strandi26 – 33Combined sources8
Helixi38 – 45Combined sources8
Beta strandi62 – 66Combined sources5
Beta strandi69 – 76Combined sources8
Beta strandi79 – 81Combined sources3
Helixi85 – 92Combined sources8
Beta strandi94 – 101Combined sources8
Helixi105 – 107Combined sources3
Helixi108 – 119Combined sources12
Helixi122 – 124Combined sources3
Beta strandi129 – 134Combined sources6
Helixi144 – 151Combined sources8
Turni154 – 156Combined sources3
Turni165 – 167Combined sources3
Beta strandi173 – 177Combined sources5
Turni180 – 183Combined sources4
Helixi186 – 194Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F6BX-ray1.70A/B1-198[»]
2FA9X-ray2.50A/B10-198[»]
2FMXX-ray1.82A/B10-198[»]
ProteinModelPortaliQ9QVY3.
SMRiQ9QVY3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QVY3.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. SAR1 family.Curated

Phylogenomic databases

HOVERGENiHBG104997.
KOiK07953.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR006689. Small_GTPase_ARF/SAR.
IPR006687. Small_GTPase_SAR1.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51422. SAR1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QVY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFIFDWIYS GFSSVLQFLG LYKKTGKLVF LGLDNAGKTT LLHMLKDDRL
60 70 80 90 100
GQHVPTLHPT SEELTIAGMT FTTFDLGGHI QARRVWKNYL PAINGIVFLV
110 120 130 140 150
DCADHERLLE SKEELDSLMT DETIANVPIL ILGNKIDRPE AISEERLREM
160 170 180 190
FGLYGQTTGK GSVSLKELNA RPLEVFMCSV LKRQGYGEGF RWMAQYID
Length:198
Mass (Da):22,410
Last modified:May 1, 2000 - v1
Checksum:i6056769070CE91FA
GO

Sequence databases

RefSeqiXP_003498539.1. XM_003498491.3.
XP_007611226.1. XM_007613036.2.
XP_007611227.1. XM_007613037.1.
XP_007626894.1. XM_007628704.2.

Genome annotation databases

GeneIDi100758826.
KEGGicge:100758826.

Cross-referencesi

Sequence databases

RefSeqiXP_003498539.1. XM_003498491.3.
XP_007611226.1. XM_007613036.2.
XP_007611227.1. XM_007613037.1.
XP_007626894.1. XM_007628704.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F6BX-ray1.70A/B1-198[»]
2FA9X-ray2.50A/B10-198[»]
2FMXX-ray1.82A/B10-198[»]
ProteinModelPortaliQ9QVY3.
SMRiQ9QVY3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100758826.
KEGGicge:100758826.

Organism-specific databases

CTDi51128.

Phylogenomic databases

HOVERGENiHBG104997.
KOiK07953.

Miscellaneous databases

EvolutionaryTraceiQ9QVY3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR006689. Small_GTPase_ARF/SAR.
IPR006687. Small_GTPase_SAR1.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51422. SAR1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAR1B_CRIGR
AccessioniPrimary (citable) accession number: Q9QVY3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.