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Protein

Transcription elongation factor A protein 2

Gene

Tcea2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri257 – 29741TFIIS-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor A protein 2
Alternative name(s):
Protein S-II-T1
Testis-specific S-II
Transcription elongation factor S-II protein 2
Transcription elongation factor TFIIS.l
Gene namesi
Name:Tcea2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:107368. Tcea2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Transcription elongation factor A protein 2PRO_0000121450Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki57 – 57Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei59 – 591PhosphoserineBy similarity
Modified residuei100 – 1001PhosphoserineBy similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9QVN7.
PaxDbiQ9QVN7.
PRIDEiQ9QVN7.

PTM databases

PhosphoSiteiQ9QVN7.

Expressioni

Tissue specificityi

Testis and ovary specific. Specific to testicular germ cells.

Developmental stagei

Expressed in testicular germ cells during and after meiosis in the course of spermatogenesis, while it is not expressed in premeiotic or early meiotic testicular germ cells.

Gene expression databases

BgeeiQ9QVN7.
CleanExiMM_TCEA2.
ExpressionAtlasiQ9QVN7. baseline and differential.
GenevisibleiQ9QVN7. MM.

Interactioni

Subunit structurei

Interacts with the basal transcription factor GTF2B. Interacts with REXO1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099331.

Structurei

3D structure databases

ProteinModelPortaliQ9QVN7.
SMRiQ9QVN7. Positions 4-80, 131-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 8278TFIIS N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini138 – 254117TFIIS centralPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TFS-II family.Curated
Contains 1 TFIIS central domain.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri257 – 29741TFIIS-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1105. Eukaryota.
COG1594. LUCA.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiQ9QVN7.
KOiK03145.
OMAiVTICAYC.
OrthoDBiEOG7GQXWQ.
PhylomeDBiQ9QVN7.
TreeFamiTF314970.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QVN7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKEEEIARI ARRLDKMVTR KNAEGAMDLL RELKNMPITL HLLQSTRVGM
60 70 80 90 100
SVNALRKQSS DEELIALAKS LIKSWKKLLD VSDGKSRNQG RGTPLPTSSS
110 120 130 140 150
KDASRTTDLS CKKPDPPRTP STPRITTFPQ VPITCDAVRN KCREMLTLAL
160 170 180 190 200
QTDHDHVAVG VNCEHLSSQI EECIFLDVGN TDMKYKNRVR SRISNLKDAK
210 220 230 240 250
NPGLRRNVLC GAITPQQIAV MTSEEMASDE LKEIRKAMTK EAIREHQMAR
260 270 280 290
TGGTQTDLFT CNKCRKKNCT YTQVQTRSSD EPMTTYVVCN ECGNRWKFC
Length:299
Mass (Da):33,663
Last modified:February 12, 2003 - v2
Checksum:iF89A30B7984EEDDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86081 mRNA. Translation: BAA19752.1.
AB010271 mRNA. Translation: BAA82313.1.
CCDSiCCDS17219.1.
PIRiJC5430.
RefSeqiNP_033352.1. NM_009326.2.
UniGeneiMm.24245.

Genome annotation databases

EnsembliENSMUST00000103042; ENSMUSP00000099331; ENSMUSG00000059540.
GeneIDi21400.
KEGGimmu:21400.
UCSCiuc012cmu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86081 mRNA. Translation: BAA19752.1.
AB010271 mRNA. Translation: BAA82313.1.
CCDSiCCDS17219.1.
PIRiJC5430.
RefSeqiNP_033352.1. NM_009326.2.
UniGeneiMm.24245.

3D structure databases

ProteinModelPortaliQ9QVN7.
SMRiQ9QVN7. Positions 4-80, 131-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000099331.

PTM databases

PhosphoSiteiQ9QVN7.

Proteomic databases

EPDiQ9QVN7.
PaxDbiQ9QVN7.
PRIDEiQ9QVN7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103042; ENSMUSP00000099331; ENSMUSG00000059540.
GeneIDi21400.
KEGGimmu:21400.
UCSCiuc012cmu.2. mouse.

Organism-specific databases

CTDi6919.
MGIiMGI:107368. Tcea2.

Phylogenomic databases

eggNOGiKOG1105. Eukaryota.
COG1594. LUCA.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiQ9QVN7.
KOiK03145.
OMAiVTICAYC.
OrthoDBiEOG7GQXWQ.
PhylomeDBiQ9QVN7.
TreeFamiTF314970.

Miscellaneous databases

PROiQ9QVN7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QVN7.
CleanExiMM_TCEA2.
ExpressionAtlasiQ9QVN7. baseline and differential.
GenevisibleiQ9QVN7. MM.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Restricted expression of a member of the transcription elongation factor S-II family in testicular germ cells during and after meiosis."
    Umehara T., Kida S., Hasegawa S., Fujimoto H., Horikoshi M.
    J. Biochem. 121:598-603(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Gene organization and chromosome mapping of the testis-specific S-II."
    Ito T., Seldin M.F., Taketo M.M., Kubo T., Natori S.
    Mamm. Genome 9:915-917(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ddY.
    Tissue: Testis.
  3. "Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II."
    Ito T., Xu Q., Takeuchi H., Kubo T., Natori S.
    FEBS Lett. 385:21-24(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 80-190.

Entry informationi

Entry nameiTCEA2_MOUSE
AccessioniPrimary (citable) accession number: Q9QVN7
Secondary accession number(s): O08667
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: February 12, 2003
Last modified: July 6, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.