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Protein

Semaphorin-7A

Gene

Sema7a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.3 Publications

GO - Molecular functioni

  1. integrin binding Source: UniProtKB
  2. receptor activity Source: InterPro

GO - Biological processi

  1. axon extension Source: MGI
  2. inflammatory response Source: UniProtKB-KW
  3. integrin-mediated signaling pathway Source: UniProtKB
  4. neuron projection development Source: MGI
  5. olfactory lobe development Source: MGI
  6. osteoblast differentiation Source: MGI
  7. positive regulation of axon extension Source: UniProtKB
  8. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  9. positive regulation of macrophage cytokine production Source: UniProtKB
  10. positive regulation of protein phosphorylation Source: MGI
  11. regulation of inflammatory response Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Inflammatory response, Neurogenesis

Enzyme and pathway databases

ReactomeiREACT_280605. Other semaphorin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Semaphorin-7A
Alternative name(s):
Semaphorin-K1
Short name:
Sema K1
Semaphorin-L
Short name:
Sema L
CD_antigen: CD108
Gene namesi
Name:Sema7a
Synonyms:Cd108, Semal, Semk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1306826. Sema7a.

Subcellular locationi

Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication; Extracellular side 1 Publication

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. external side of plasma membrane Source: UniProtKB
  3. membrane Source: MGI
  4. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice display normal levels of lymphocytes in spleen, and normal activation of T-cells by antigenic stimuli. In contrast, production of proinflammatory cytokines by macrophages is much reduced. The effect on contact hypersensitivity and experimental autoimmune encephalomyelitis is controversial. Reduced size of the lateral olfactory tract.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4444Sequence AnalysisAdd
BLAST
Chaini45 – 646602Semaphorin-7APRO_0000032349Add
BLAST
Propeptidei647 – 66418Removed in mature formSequence AnalysisPRO_0000032350Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi102 – 1021N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi117 ↔ 123By similarity
Disulfide bondi140 ↔ 149By similarity
Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi264 ↔ 364By similarity
Disulfide bondi289 ↔ 333By similarity
Glycosylationi328 – 3281N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi491 ↔ 509By similarity
Disulfide bondi498 ↔ 539By similarity
Disulfide bondi501 ↔ 516By similarity
Disulfide bondi564 ↔ 611By similarity
Disulfide bondi585 ↔ 594By similarity
Glycosylationi600 – 6001N-linked (GlcNAc...)Sequence Analysis
Lipidationi646 – 6461GPI-anchor amidated alanineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiQ9QUR8.
PaxDbiQ9QUR8.
PRIDEiQ9QUR8.

PTM databases

PhosphoSiteiQ9QUR8.

Expressioni

Tissue specificityi

Highly expressed in activated T-cells (at protein level). Highest expression in brain. Lower in heart, thymus, spleen, testis and ovary. The expression increases in late embryonic and postnatal stages. Detected in T-cells.2 Publications

Gene expression databases

BgeeiQ9QUR8.
GenevestigatoriQ9QUR8.

Interactioni

Subunit structurei

Interacts with PLXNC1 (By similarity). Interacts with ITGA1 and ITGB1.By similarity1 Publication

Protein-protein interaction databases

IntActiQ9QUR8. 1 interaction.
MINTiMINT-4134084.

Structurei

3D structure databases

ProteinModelPortaliQ9QUR8.
SMRiQ9QUR8. Positions 46-631.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 488436SemaPROSITE-ProRule annotationAdd
BLAST
Domaini542 – 62786Ig-like C2-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni265 – 2673Interaction with integrinsBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi265 – 2673Cell attachment siteSequence Analysis

Sequence similaritiesi

Belongs to the semaphorin family.Curated
Contains 1 PSI domain.Curated
Contains 1 Sema domain.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Signal

Phylogenomic databases

eggNOGiNOG277924.
GeneTreeiENSGT00760000119134.
HOGENOMiHOG000154284.
HOVERGENiHBG079171.
InParanoidiQ9QUR8.
KOiK06529.
OMAiEPMGPLK.
OrthoDBiEOG754HNX.
PhylomeDBiQ9QUR8.
TreeFamiTF333698.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR016201. Plexin-like_fold.
IPR002165. Plexin_repeat.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR11036. PTHR11036. 1 hit.
PfamiPF01437. PSI. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QUR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPPPPGRAA PSAPRARVLS LPARFGLPLR LRLLLVFWVA AASAQGHSRS
60 70 80 90 100
GPRISAVWKG QDHVDFSQPE PHTVLFHEPG SFSVWVGGRG KVYHFNFPEG
110 120 130 140 150
KNASVRTVNI GSTKGSCQDK QDCGNYITLL ERRGNGLLVC GTNARKPSCW
160 170 180 190 200
NLVNDSVVMS LGEMKGYAPF SPDENSLVLF EGDEVYSTIR KQEYNGKIPR
210 220 230 240 250
FRRIRGESEL YTSDTVMQNP QFIKATIVHQ DQAYDDKIYY FFREDNPDKN
260 270 280 290 300
PEAPLNVSRV AQLCRGDQGG ESSLSVSKWN TFLKAMLVCS DAATNRNFNR
310 320 330 340 350
LQDVFLLPDP SGQWRDTRVY GVFSNPWNYS AVCVYSLGDI DRVFRTSSLK
360 370 380 390 400
GYHMGLPNPR PGMCLPKKQP IPTETFQVAD SHPEVAQRVE PMGPLKTPLF
410 420 430 440 450
HSKYHYQKVV VHRMQASNGE TFHVLYLTTD RGTIHKVVES GDQDHSFVFN
460 470 480 490 500
IMEIQPFHRA AAIQAISLDA DRRKLYVTSQ WEVSQVPLDM CEVYSGGCHG
510 520 530 540 550
CLMSRDPYCG WDQDRCVSIY SSQRSVLQSI NPAEPHRECP NPKPDEAPLQ
560 570 580 590 600
KVSLARNSRY YLTCPMESRH ATYLWRHEEN VEQSCEPGHQ SPSCILFIEN
610 620 630 640 650
LTARQYGHYR CEAQEGSYLR EAQHWELLPE DRALAEQLMG HARALAASFW
660
LGVLPTLILG LLVH
Length:664
Mass (Da):74,994
Last modified:April 30, 2000 - v1
Checksum:i1804A31C3C55B715
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti357 – 3571P → S in AAC34262 (PubMed:9721204).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017532 mRNA. Translation: BAA75665.1.
AF030699 mRNA. Translation: AAC34262.1.
AF176670 mRNA. Translation: AAD53118.1.
BC057875 mRNA. Translation: AAH57875.1.
CCDSiCCDS23235.1.
RefSeqiNP_035482.1. NM_011352.2.
UniGeneiMm.335187.

Genome annotation databases

EnsembliENSMUST00000043059; ENSMUSP00000042211; ENSMUSG00000038264.
GeneIDi20361.
KEGGimmu:20361.
UCSCiuc009pvy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017532 mRNA. Translation: BAA75665.1.
AF030699 mRNA. Translation: AAC34262.1.
AF176670 mRNA. Translation: AAD53118.1.
BC057875 mRNA. Translation: AAH57875.1.
CCDSiCCDS23235.1.
RefSeqiNP_035482.1. NM_011352.2.
UniGeneiMm.335187.

3D structure databases

ProteinModelPortaliQ9QUR8.
SMRiQ9QUR8. Positions 46-631.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QUR8. 1 interaction.
MINTiMINT-4134084.

PTM databases

PhosphoSiteiQ9QUR8.

Proteomic databases

MaxQBiQ9QUR8.
PaxDbiQ9QUR8.
PRIDEiQ9QUR8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043059; ENSMUSP00000042211; ENSMUSG00000038264.
GeneIDi20361.
KEGGimmu:20361.
UCSCiuc009pvy.1. mouse.

Organism-specific databases

CTDi8482.
MGIiMGI:1306826. Sema7a.

Phylogenomic databases

eggNOGiNOG277924.
GeneTreeiENSGT00760000119134.
HOGENOMiHOG000154284.
HOVERGENiHBG079171.
InParanoidiQ9QUR8.
KOiK06529.
OMAiEPMGPLK.
OrthoDBiEOG754HNX.
PhylomeDBiQ9QUR8.
TreeFamiTF333698.

Enzyme and pathway databases

ReactomeiREACT_280605. Other semaphorin interactions.

Miscellaneous databases

NextBioi298249.
PROiQ9QUR8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QUR8.
GenevestigatoriQ9QUR8.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR016201. Plexin-like_fold.
IPR002165. Plexin_repeat.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR11036. PTHR11036. 1 hit.
PfamiPF01437. PSI. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMiSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of murine homologue of semaphorin K1 gene."
    Sato Y., Takahashi H.
    Biochim. Biophys. Acta 1443:419-422(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "New eukaryotic semaphorins with close homology to semaphorins of DNA viruses."
    Lange C., Liehr T., Goen M., Gebhart E., Fleckenstein B., Ensser A.
    Genomics 51:340-350(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: 129.
    Tissue: Mammary gland.
  5. "Semaphorin 7A promotes axon outgrowth through integrins and MAPKs."
    Pasterkamp R.J., Peschon J.J., Spriggs M.K., Kolodkin A.L.
    Nature 424:398-405(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  6. "Semaphorin 7A is a negative regulator of T cell responses."
    Czopik A.K., Bynoe M.S., Palm N., Raine C.S., Medzhitov R.
    Immunity 24:591-600(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY.
  7. "Semaphorin 7A initiates T-cell-mediated inflammatory responses through alpha1beta1 integrin."
    Suzuki K., Okuno T., Yamamoto M., Pasterkamp R.J., Takegahara N., Takamatsu H., Kitao T., Takagi J., Rennert P.D., Kolodkin A.L., Kumanogoh A., Kikutani H.
    Nature 446:680-684(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INTERACTION WITH ITGA1 AND ITGB1, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSEM7A_MOUSE
AccessioniPrimary (citable) accession number: Q9QUR8
Secondary accession number(s): O88371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 19, 2001
Last sequence update: April 30, 2000
Last modified: March 31, 2015
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

The exact role in regulating T-cell function is under debate. According to (PubMed:16713976), SEMA7A-deficient mice are highly susceptible to contact hypersensitivity and experimental autoimmune encephalomyelitis. According to (PubMed:17377534) mice do not develop contact hypersensitivity, and are highly resistant to experimental autoimmune encephalomyelitis.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.