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Q9QUR8 (SEM7A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Semaphorin-7A
Alternative name(s):
Semaphorin-K1
Short name=Sema K1
Semaphorin-L
Short name=Sema L
CD_antigen=CD108
Gene names
Name:Sema7a
Synonyms:Cd108, Semal, Semk1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length664 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. Ref.5 Ref.6 Ref.7

Subunit structure

Interacts with PLXNC1 By similarity. Interacts with ITGA1 and ITGB1. Ref.7

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor; Extracellular side Ref.7.

Tissue specificity

Highly expressed in activated T-cells (at protein level). Highest expression in brain. Lower in heart, thymus, spleen, testis and ovary. The expression increases in late embryonic and postnatal stages. Detected in T-cells. Ref.6 Ref.7

Disruption phenotype

No visible phenotype. Mice display normal levels of lymphocytes in spleen, and normal activation of T-cells by antigenic stimuli. In contrast, production of proinflammatory cytokines by macrophages is much reduced. The effect on contact hypersensitivity and experimental autoimmune encephalomyelitis is controversial. Reduced size of the lateral olfactory tract. Ref.5 Ref.6 Ref.7

Sequence similarities

Belongs to the semaphorin family.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 PSI domain.

Contains 1 Sema domain.

Caution

The exact role in regulating T-cell function is under debate. According to (Ref.6), SEMA7A-deficient mice are highly susceptible to contact hypersensitivity and experimental autoimmune encephalomyelitis. According to (Ref.7) mice do not develop contact hypersensitivity, and are highly resistant to experimental autoimmune encephalomyelitis.

Ontologies

Keywords
   Biological processDifferentiation
Inflammatory response
Neurogenesis
   Cellular componentCell membrane
Membrane
   DomainImmunoglobulin domain
Signal
   Molecular functionDevelopmental protein
   PTMDisulfide bond
Glycoprotein
GPI-anchor
Lipoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaxon extension

Inferred from direct assay Ref.5. Source: MGI

inflammatory response

Inferred from electronic annotation. Source: UniProtKB-KW

integrin-mediated signaling pathway

Inferred from direct assay Ref.7. Source: UniProtKB

neuron projection development

Inferred from mutant phenotype Ref.5. Source: MGI

olfactory lobe development

Inferred from direct assay Ref.5. Source: MGI

positive regulation of ERK1 and ERK2 cascade

Inferred from direct assay Ref.7. Source: UniProtKB

positive regulation of axon extension

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of macrophage cytokine production

Inferred from direct assay Ref.7. Source: UniProtKB

positive regulation of protein phosphorylation

Inferred from direct assay Ref.5. Source: MGI

regulation of inflammatory response

Inferred from direct assay Ref.7. Source: UniProtKB

   Cellular_componentanchored component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

external side of plasma membrane

Inferred from direct assay Ref.7. Source: UniProtKB

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionintegrin binding

Inferred from physical interaction Ref.7. Source: UniProtKB

receptor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4444 Potential
Chain45 – 646602Semaphorin-7A
PRO_0000032349
Propeptide647 – 66418Removed in mature form Potential
PRO_0000032350

Regions

Domain53 – 488436Sema
Domain542 – 62786Ig-like C2-type
Region265 – 2673Interaction with integrins By similarity
Motif265 – 2673Cell attachment site Potential

Amino acid modifications

Lipidation6461GPI-anchor amidated alanine Potential
Glycosylation1021N-linked (GlcNAc...) Potential
Glycosylation1541N-linked (GlcNAc...) Potential
Glycosylation2561N-linked (GlcNAc...) Potential
Glycosylation3281N-linked (GlcNAc...) Potential
Glycosylation6001N-linked (GlcNAc...) Potential
Disulfide bond117 ↔ 123 By similarity
Disulfide bond140 ↔ 149 By similarity
Disulfide bond264 ↔ 364 By similarity
Disulfide bond289 ↔ 333 By similarity
Disulfide bond491 ↔ 509 By similarity
Disulfide bond498 ↔ 539 By similarity
Disulfide bond501 ↔ 516 By similarity
Disulfide bond564 ↔ 611 By similarity
Disulfide bond585 ↔ 594 By similarity

Experimental info

Sequence conflict3571P → S in AAC34262. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9QUR8 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 1804A31C3C55B715

FASTA66474,994
        10         20         30         40         50         60 
MTPPPPGRAA PSAPRARVLS LPARFGLPLR LRLLLVFWVA AASAQGHSRS GPRISAVWKG 

        70         80         90        100        110        120 
QDHVDFSQPE PHTVLFHEPG SFSVWVGGRG KVYHFNFPEG KNASVRTVNI GSTKGSCQDK 

       130        140        150        160        170        180 
QDCGNYITLL ERRGNGLLVC GTNARKPSCW NLVNDSVVMS LGEMKGYAPF SPDENSLVLF 

       190        200        210        220        230        240 
EGDEVYSTIR KQEYNGKIPR FRRIRGESEL YTSDTVMQNP QFIKATIVHQ DQAYDDKIYY 

       250        260        270        280        290        300 
FFREDNPDKN PEAPLNVSRV AQLCRGDQGG ESSLSVSKWN TFLKAMLVCS DAATNRNFNR 

       310        320        330        340        350        360 
LQDVFLLPDP SGQWRDTRVY GVFSNPWNYS AVCVYSLGDI DRVFRTSSLK GYHMGLPNPR 

       370        380        390        400        410        420 
PGMCLPKKQP IPTETFQVAD SHPEVAQRVE PMGPLKTPLF HSKYHYQKVV VHRMQASNGE 

       430        440        450        460        470        480 
TFHVLYLTTD RGTIHKVVES GDQDHSFVFN IMEIQPFHRA AAIQAISLDA DRRKLYVTSQ 

       490        500        510        520        530        540 
WEVSQVPLDM CEVYSGGCHG CLMSRDPYCG WDQDRCVSIY SSQRSVLQSI NPAEPHRECP 

       550        560        570        580        590        600 
NPKPDEAPLQ KVSLARNSRY YLTCPMESRH ATYLWRHEEN VEQSCEPGHQ SPSCILFIEN 

       610        620        630        640        650        660 
LTARQYGHYR CEAQEGSYLR EAQHWELLPE DRALAEQLMG HARALAASFW LGVLPTLILG 


LLVH 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of murine homologue of semaphorin K1 gene."
Sato Y., Takahashi H.
Biochim. Biophys. Acta 1443:419-422(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"New eukaryotic semaphorins with close homology to semaphorins of DNA viruses."
Lange C., Liehr T., Goen M., Gebhart E., Fleckenstein B., Ensser A.
Genomics 51:340-350(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[3]"CDw108 expression during T-cell development."
Mine T., Harada K., Matsumoto T., Yamana H., Shirouzu K., Itoh K., Yamada A.
Tissue Antigens 55:429-436(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
Tissue: Embryo.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: 129.
Tissue: Mammary gland.
[5]"Semaphorin 7A promotes axon outgrowth through integrins and MAPKs."
Pasterkamp R.J., Peschon J.J., Spriggs M.K., Kolodkin A.L.
Nature 424:398-405(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
[6]"Semaphorin 7A is a negative regulator of T cell responses."
Czopik A.K., Bynoe M.S., Palm N., Raine C.S., Medzhitov R.
Immunity 24:591-600(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY.
[7]"Semaphorin 7A initiates T-cell-mediated inflammatory responses through alpha1beta1 integrin."
Suzuki K., Okuno T., Yamamoto M., Pasterkamp R.J., Takegahara N., Takamatsu H., Kitao T., Takagi J., Rennert P.D., Kolodkin A.L., Kumanogoh A., Kikutani H.
Nature 446:680-684(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INTERACTION WITH ITGA1 AND ITGB1, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB017532 mRNA. Translation: BAA75665.1.
AF030699 mRNA. Translation: AAC34262.1.
AF176670 mRNA. Translation: AAD53118.1.
BC057875 mRNA. Translation: AAH57875.1.
CCDSCCDS23235.1.
RefSeqNP_035482.1. NM_011352.2.
UniGeneMm.335187.

3D structure databases

ProteinModelPortalQ9QUR8.
SMRQ9QUR8. Positions 46-631.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9QUR8. 1 interaction.
MINTMINT-4134084.

PTM databases

PhosphoSiteQ9QUR8.

Proteomic databases

PaxDbQ9QUR8.
PRIDEQ9QUR8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043059; ENSMUSP00000042211; ENSMUSG00000038264.
GeneID20361.
KEGGmmu:20361.
UCSCuc009pvy.1. mouse.

Organism-specific databases

CTD8482.
MGIMGI:1306826. Sema7a.

Phylogenomic databases

eggNOGNOG277924.
GeneTreeENSGT00690000101974.
HOGENOMHOG000154284.
HOVERGENHBG079171.
InParanoidQ9QUR8.
KOK06529.
OMAEPMGPLK.
OrthoDBEOG754HNX.
PhylomeDBQ9QUR8.
TreeFamTF333698.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.

Gene expression databases

BgeeQ9QUR8.
GenevestigatorQ9QUR8.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR016201. Plexin-like_fold.
IPR002165. Plexin_repeat.
IPR001627. Semap_dom.
IPR027231. Semaphorin.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERPTHR11036. PTHR11036. 1 hit.
PfamPF01437. PSI. 1 hit.
PF01403. Sema. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
SM00423. PSI. 1 hit.
SM00630. Sema. 1 hit.
[Graphical view]
SUPFAMSSF101912. SSF101912. 1 hit.
SSF103575. SSF103575. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS51004. SEMA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298249.
PROQ9QUR8.
SOURCESearch...

Entry information

Entry nameSEM7A_MOUSE
AccessionPrimary (citable) accession number: Q9QUR8
Secondary accession number(s): O88371
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot