Reviewed, UniProtKB/Swiss-Prot Q9QUR6 (PPCE_MOUSE)

Names and origin

Protein names

Recommended name:
    Prolyl endopeptidase
      Short name=PE
    EC=3.4.21.26
Alternative name(s):
    Post-proline cleaving enzyme

Gene names

Name:	Prep
Synonyms:	Pep

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	710 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.
Catalytic activity	Hydrolysis of Pro-|-Xaa >> Ala-|-Xaa in oligopeptides.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the peptidase S9A family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Serine protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: MGI nucleus Ref.1 Inferred from direct assay. Source: MGI
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 710	710	Prolyl endopeptidase		PRO_0000122402
Sites
	Active site	554	1	Charge relay system By similarity
	Active site	641	1	Charge relay system By similarity
	Active site	680	1	Charge relay system By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QUR6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 1B010D5D6CA73C0E	FASTA	710	80,752
	10 20 30 40 50 60 MLSFQYPDVY RDETSVQEYH GHKICDPYSW LEDPDSEQTK AFVEAQNKIT VPFLEQCPIR 70 80 90 100 110 120 GLYKERMTEL YDYPKYSCHF KKGKRYFYFY NTGLQNQRVL YVQDSLEGEA RVFLDPNTLS 130 140 150 160 170 180 DDGTVALRGY AFSEDGEYFA YGLSASGSDW VTIKFMKVDG AKELPDVLER VKFTCMAWTH 190 200 210 220 230 240 DGKGMFYNSY PQQDGKSDGT ETSTNLHQKL CYHVLGTDQS EDILCAEFPD EPKWMGGAEL 250 260 270 280 290 300 SDDGRYVLLS IWEGCDPVNR LWYCDLQQEP NGITGILKWV KLIDNFEGEY DYVTNEGTVF 310 320 330 340 350 360 TFKTNRNSPN YRLINIDFTD PDESKWKVLV PEHEKDVLEW VACVRSNFLV LCYLHDVKNI 370 380 390 400 410 420 LQLHDLTTGA LLKTFPLDVG SVVGYSGRKK DSEIFYQFTS FLSPGVIYHC DLTKEELEPM 430 440 450 460 470 480 VFREVTVKGI DAADYQTIQI FYPSKDGTKI PMFIVHKKGI KLDGSHPAFL YGYGGFNISI 490 500 510 520 530 540 TPNYSVSRLI FVRHMGGVLA VANIRGGGEY GETWHKGGIL ANKQNCFDDF QCAAEYLIKE 550 560 570 580 590 600 GYTSPKRLTI NGGSNGGLLV AACANQRPDL FGCVIAQVGV MDMLKFHKFT IGHAWTTDYG 610 620 630 640 650 660 CSDTKQHFEW LLKYSPLHNV KLPEADDIQY PSMLLLTADH DDRVVPLHSL KFIATLQYIV 670 680 690 700 710 GRSRKQSNPL LIHVDTKAGH GAGKPTAKVI EEVSDMFAFI ARCLNIEWIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of mouse prolyl endopeptidase and its involvement in DNA synthesis by Swiss 3T3 cells." Ishino T., Ohtsuki S., Homma K., Natori S. J. Biochem. 123:540-545(1998) [PubMed: 9538240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Structure and localization of the mouse prolyl oligopeptidase gene." Kimura A., Yoshida I., Takagi N., Takahashi T. J. Biol. Chem. 274:24047-24053(1999) [PubMed: 10446174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Limb and Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB007631 mRNA. Translation: BAA88239.1. AB022053 Genomic DNA. Translation: BAA83071.1. BC012869 mRNA. Translation: AAH12869.1. BC050830 mRNA. Translation: AAH50830.2.
IPI	IPI00132089.
PIR	JW0080.
RefSeq	NP_035286.1.
UniGene	Mm.37294
3D structure databases
HSSP	HSSP built from PDB template 1O6G based on UniProtKB P23687.
SMR	Q9QUR6. Positions 1-709.
ModBase	Search...
Protein family/group databases
MEROPS	S09.001.
PTM databases
PhosphoSite	Q9QUR6.
Proteomic databases
PRIDE	Q9QUR6.
Genome annotation databases
Ensembl	ENSMUSG00000019849. Mus musculus. [Contig view]
GeneID	19072.
KEGG	mmu:19072.
Organism-specific databases
MGI	MGI:1270863. Prep.
Phylogenomic databases
HOVERGEN	Q9QUR6.
Enzyme and pathway databases
BRENDA	3.4.21.26. 244.
Gene expression databases
ArrayExpress	Q9QUR6.
Bgee	Q9QUR6.
CleanEx	MM_PREP.
GermOnline	ENSMUSG00000019849. Mus musculus.
Family and domain databases
InterPro	IPR002471. Pept_S9_AS. IPR001375. Peptidase_S9. IPR002470. Peptidase_S9A. IPR004106. Peptidase_S9A_N. [Graphical view]
PANTHER	PTHR11757. Peptidase_S9A. 1 hit.
Pfam	PF00326. Peptidase_S9. 1 hit. PF02897. Peptidase_S9_N. 1 hit. [Graphical view]
PRINTS	PR00862. PROLIGOPTASE.
PROSITE	PS00708. PRO_ENDOPEP_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	295594.
SOURCE	Search...

Entry information

Entry name

PPCE_MOUSE

Accession

Primary (citable) accession number: Q9QUR6
Secondary accession number(s): Q80YS1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	February 10, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)