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Protein

Dynactin subunit 4

Gene

Dctn4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-RNO-2132295. MHC class II antigen presentation.
R-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Names & Taxonomyi

Protein namesi
Recommended name:
Dynactin subunit 4
Alternative name(s):
Dynactin subunit p62
Gene namesi
Name:Dctn4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi71023. Dctn4.

Subcellular locationi

  • Cytoplasmcell cortex 1 Publication
  • Cytoplasmcytoskeleton 1 Publication

  • Note: In skeletal muscles, demonstrates a punctate distribution along costameres (By similarity). Overexpression in cultured mammalian cells revealed colocalization with cortical actin, stress fibers, and focal adhesion sites, sites of potential interaction between microtubules and the cell cortex. Also colocalized with polymers of overexpressed wild-type or barbed-end-mutant Arp1, but not with a pointed-end mutant.By similarity

GO - Cellular componenti

  • cell cortex Source: UniProtKB-SubCell
  • centrosome Source: Ensembl
  • cytoplasmic dynein complex Source: RGD
  • dynactin complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 467466Dynactin subunit 4PRO_0000079826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei203 – 2031PhosphoserineBy similarity
Modified residuei414 – 4141PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9QUR2.
PRIDEiQ9QUR2.

PTM databases

iPTMnetiQ9QUR2.
PhosphoSiteiQ9QUR2.

Expressioni

Gene expression databases

ExpressionAtlasiQ9QUR2. baseline.

Interactioni

Subunit structurei

Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10 (By similarity). Interacts with ATP7B, but not ATP7A, in a copper-dependent manner (By similarity). Interacts with ANK2; this interaction is required for localization at costameres (By similarity). Binds directly to the ACTR1A subunit of dynactin.By similarity

Protein-protein interaction databases

IntActiQ9QUR2. 1 interaction.
STRINGi10116.ENSRNOP00000026394.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili152 – 17221Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the dynactin subunit 4 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3896. Eukaryota.
ENOG410ZCK8. LUCA.
GeneTreeiENSGT00390000006954.
HOGENOMiHOG000007947.
HOVERGENiHBG051324.
InParanoidiQ9QUR2.
KOiK10426.
OMAiPIRPMEE.
OrthoDBiEOG7SXW2X.
PhylomeDBiQ9QUR2.
TreeFamiTF105249.

Family and domain databases

InterProiIPR008603. DCTN4.
[Graphical view]
PANTHERiPTHR13034. PTHR13034. 1 hit.
PfamiPF05502. Dynactin_p62. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QUR2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLLQSERV LYLVQGEKKV RAPLSQLYFC RYCSELRSLE CVSHEVDSHY
60 70 80 90 100
CPSCLENMPS AEAKLKKNRC ANCFDCPGCM HTLSTRATSI STQLPDDPAK
110 120 130 140 150
TTMKKAYYLA CGFCRWTSRD VGMADKSVAS GGWQEPENPH AQRMNKLIEY
160 170 180 190 200
YQQLAQKEKV ERDRKKLARR RNYMPLAFSQ HTIHVVDKYS LGTRLQRPRA
210 220 230 240 250
GASISTLAGL SLREGEDQKE VKIEPAQAVA EVEPLPEDYY TRPVNLTEVT
260 270 280 290 300
TLQQRLLQPD LQPVSASQLY PRHKHLLIKR SLRCRKCEHN LSKPEFNPTS
310 320 330 340 350
IKFKIQLVAV NYIPEVRIMS IPNLRYMKES QVLLTLTNPV ENLTHVTLLE
360 370 380 390 400
CEEGDPDNIN STAKVVVPPK ELILAGKDAA AEYDELAEPQ DFQDDPDIVA
410 420 430 440 450
FRKANKVGIF IKVTPQREEG DVTVCFKMKH DFKNLAAPIR PMEESDQGTE
460
VIWLTQHVEL SFGPLLP
Length:467
Mass (Da):53,089
Last modified:May 1, 2000 - v1
Checksum:i2134F40E21108E08
GO
Isoform 2 (identifier: Q9QUR2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     180-186: Missing.

Show »
Length:460
Mass (Da):52,274
Checksum:i70CAF165AF9BA903
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei180 – 1867Missing in isoform 2. 1 PublicationVSP_013574

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF190798 mRNA. Translation: AAF05618.1.
AF192493 mRNA. Translation: AAF03421.1.
AF192494 mRNA. Translation: AAF03422.1.
RefSeqiNP_445856.1. NM_053404.2. [Q9QUR2-1]
XP_006254845.1. XM_006254783.2. [Q9QUR2-2]
UniGeneiRn.23941.

Genome annotation databases

EnsembliENSRNOT00000026334; ENSRNOP00000026334; ENSRNOG00000019298. [Q9QUR2-2]
ENSRNOT00000026394; ENSRNOP00000026394; ENSRNOG00000019298. [Q9QUR2-1]
GeneIDi84428.
KEGGirno:84428.
UCSCiRGD:71023. rat. [Q9QUR2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF190798 mRNA. Translation: AAF05618.1.
AF192493 mRNA. Translation: AAF03421.1.
AF192494 mRNA. Translation: AAF03422.1.
RefSeqiNP_445856.1. NM_053404.2. [Q9QUR2-1]
XP_006254845.1. XM_006254783.2. [Q9QUR2-2]
UniGeneiRn.23941.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QUR2. 1 interaction.
STRINGi10116.ENSRNOP00000026394.

PTM databases

iPTMnetiQ9QUR2.
PhosphoSiteiQ9QUR2.

Proteomic databases

PaxDbiQ9QUR2.
PRIDEiQ9QUR2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026334; ENSRNOP00000026334; ENSRNOG00000019298. [Q9QUR2-2]
ENSRNOT00000026394; ENSRNOP00000026394; ENSRNOG00000019298. [Q9QUR2-1]
GeneIDi84428.
KEGGirno:84428.
UCSCiRGD:71023. rat. [Q9QUR2-1]

Organism-specific databases

CTDi51164.
RGDi71023. Dctn4.

Phylogenomic databases

eggNOGiKOG3896. Eukaryota.
ENOG410ZCK8. LUCA.
GeneTreeiENSGT00390000006954.
HOGENOMiHOG000007947.
HOVERGENiHBG051324.
InParanoidiQ9QUR2.
KOiK10426.
OMAiPIRPMEE.
OrthoDBiEOG7SXW2X.
PhylomeDBiQ9QUR2.
TreeFamiTF105249.

Enzyme and pathway databases

ReactomeiR-RNO-2132295. MHC class II antigen presentation.
R-RNO-6807878. COPI-mediated anterograde transport.
R-RNO-6811436. COPI-independent Golgi-to-ER retrograde traffic.

Miscellaneous databases

PROiQ9QUR2.

Gene expression databases

ExpressionAtlasiQ9QUR2. baseline.

Family and domain databases

InterProiIPR008603. DCTN4.
[Graphical view]
PANTHERiPTHR13034. PTHR13034. 1 hit.
PfamiPF05502. Dynactin_p62. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of dynactin subcomplexes reveals a novel actin-related protein associated with the Arp1 minifilament pointed end."
    Eckley D.M., Gill S.R., Melkonian K.A., Bingham J.B., Goodson H.V., Heuser J.E., Schroer T.A.
    J. Cell Biol. 147:307-320(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Interaction of the p62 subunit of dynactin with Arp1 and the cortical actin cytoskeleton."
    Garces J.A., Clark I.B., Meyer D.I., Vallee R.B.
    Curr. Biol. 9:1497-1500(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, POSSIBLE FUNCTION.
    Tissue: Brain.
  3. "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."
    Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.
    Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-414, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDCTN4_RAT
AccessioniPrimary (citable) accession number: Q9QUR2
Secondary accession number(s): Q9QXP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.