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Q9QUQ5 (TRPC4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Short transient receptor potential channel 4
Short name=TrpC4
Alternative name(s):
Capacitative calcium entry channel Trp4
Receptor-activated cation channel TRP4
Gene names
Name:Trpc4
Synonyms:Trrp4
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length974 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective By similarity. May also be activated by intracellular calcium store depletion. Trpc4 deficient mice lack a store-operated calcium entry in endothelial cells. Ref.6

Subunit structure

Homotetramer. Heterotetramer with TRPC1 and/or TRPC5 By similarity. Isoform alpha but not isoform beta associates with inositol-1,4,5-triphosphate receptor (ITPR) By similarity. Interacts with NHERF. Interacts with MX1 and RNF24 By similarity. Interacts (via CIRB domain) with SESTD1 (via the spectrin 1 repeat) and SPTBN5 (via C-terminus) By similarity. Interacts with CDH5 and CTNNB1 By similarity. Interacts (via protein 4.1-binding domain) with EPB41L2 By similarity. Interacts with TRPC4AP. Ref.7 Ref.8

Subcellular location

Membrane; Multi-pass membrane protein Probable. Cell membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Abundantly expressed in brain (hippocampal CA1 pyramidal neurons, dentate gyrus granule cells, and cerebral cortical neurons, and in the septal nuclei and the mitral layer of olfactory bulb). Lower levels are detected in other tissues.

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily. [View classification]

Contains 4 ANK repeats.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha (identifier: Q9QUQ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Beta (identifier: Q9QUQ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     781-864: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 974974Short transient receptor potential channel 4
PRO_0000215315

Regions

Topological domain1 – 329329Cytoplasmic Potential
Transmembrane330 – 35021Helical; Potential
Topological domain351 – 36212Extracellular Potential
Transmembrane363 – 38321Helical; Potential
Topological domain384 – 43653Cytoplasmic Potential
Transmembrane437 – 45721Helical; Potential
Topological domain458 – 46912Extracellular Potential
Transmembrane470 – 49021Helical; Potential
Topological domain491 – 51121Cytoplasmic Potential
Transmembrane512 – 53221Helical; Potential
Topological domain533 – 59967Extracellular Potential
Transmembrane600 – 62021Helical; Potential
Topological domain621 – 974354Cytoplasmic Potential
Repeat31 – 6030ANK 1
Repeat69 – 9729ANK 2
Repeat98 – 12427ANK 3
Repeat141 – 17030ANK 4
Region87 – 17286Multimerization domain
Region254 – 30451Multimerization domain
Region615 – 974360Binds to ITPR1, ITPR2 and ITPR3 By similarity
Region972 – 9743PDZ-binding domain By similarity
Coiled coil223 – 26038 Potential

Amino acid modifications

Modified residue1931Phosphoserine By similarity
Modified residue1951Phosphoserine By similarity
Modified residue9561Phosphotyrosine; by FYN By similarity
Modified residue9691Phosphotyrosine; by FYN By similarity

Natural variations

Alternative sequence781 – 86484Missing in isoform Beta.
VSP_006570

Experimental info

Sequence conflict7801E → K in AAF01469. Ref.3
Sequence conflict8901R → L in AAF01469. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 2D0BB2F235F5F8D1

FASTA974111,575
        10         20         30         40         50         60 
MAQFYYKRNV NAPYRDRIPL RIVRAESELS PSEKAYLNAV EKGDYASVKK SLEEAEIYFK 

        70         80         90        100        110        120 
ININCIDPLG RTALLIAIEN ENLELIELLL SFNVYVGDAL LHAIRKEVVG AVELLLNHKK 

       130        140        150        160        170        180 
PSGEKQVPPI LLDKQFSEFT PDITPIILAA HTNNYEIIKL LVQKGVSVPR PHEVRCNCVE 

       190        200        210        220        230        240 
CVSSSDVDSL RHSRSRLNIY KALASPSLIA LSSEDPFLTA FQLSWELQEL SKVENEFKSE 

       250        260        270        280        290        300 
YEELSRQCKQ FAKDLLDQTR SSRELEIILN YRDDNSLIEE QSGNDLARLK LAIKYRQKEF 

       310        320        330        340        350        360 
VAQPNCQQLL ASRWYDEFPG WRRRHWAVKM VTCFIIGLLF PVFSVCYLIA PKSPLGLFIR 

       370        380        390        400        410        420 
KPFIKFICHT ASYLTFLFLL LLASQHIDRS DLNRQGPPPT IVEWMILPWV LGFIWGEIKQ 

       430        440        450        460        470        480 
MWDGGLQDYI HDWWNLMDFV MNSLYLATIS LKIVAFVKYS ALNPRESWDM WHPTLVAEAL 

       490        500        510        520        530        540 
FAIANIFSSL RLISLFTANS HLGPLQISLG RMLLDILKFL FIYCLVLLAF ANGLNQLYFY 

       550        560        570        580        590        600 
YEETKGLSCK GIRCEKQNNA FSTLFETLQS LFWSIFGLIN LYVTNVKAQH EFTEFVGATM 

       610        620        630        640        650        660 
FGTYNVISLV VLLNMLIAMM NNSYQLIADH ADIEWKFART KLWMSYFEEG GTLPTPFNVI 

       670        680        690        700        710        720 
PSPKSLWYLV KWIWTHLCKK KMRRKPESFG TIGRRAADNL RRHHQYQEVM RNLVKRYVAA 

       730        740        750        760        770        780 
MIREAKTEEG LTEENVKELK QDISSFRFEV LGLLRGSKLS TIQSANAASS ADSDEKSQSE 

       790        800        810        820        830        840 
GNGKDKRKNL SLFDLTTLIH PRSAAIASER HNLSNGSALV VQEPPREKQR KVNFVADIKN 

       850        860        870        880        890        900 
FGLFHRRSKQ NAAEQNANQI FSVSEEITRQ QAAGALERNI ELESKGLASR GDRSIPGLNE 

       910        920        930        940        950        960 
QCVLVDHRER NTDTLGLQVG KRVCSTFKSE KVVVEDTVPI IPKEKHAHEE DSSIDYDLSP 

       970 
TDTAAHEDYV TTRL

« Hide

Isoform Beta [UniParc].

Checksum: 26901B5A81068694
Show »

FASTA890102,181

References

[1]"Trp4 is involved in capacitative calcium entry in murine cells."
Zhu X., Boulay G., Jiang M., Birnbaumer L.
Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
Tissue: Brain.
[2]Qian F., Philipson L.H.
Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
[3]"Differential distribution of TRP Ca2+ channel isoforms in mouse brain."
Mori Y., Takada N., Okada T., Wakamori M., Imoto K., Wanifuchi H., Oka H., Oba A., Ikenaka K., Kurosaki T.
NeuroReport 9:507-515(1998) [PubMed: 9512398] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
Tissue: Brain.
[4]"Association of mammalian trp4 and phospholipase C isozymes with a PDZ domain-containing protein, NHERF."
Tang Y., Tang J., Chen Z., Trost C., Flockerzi V., Li M., Ramesh V., Zhu M.X.
J. Biol. Chem. 275:37559-37564(2000) [PubMed: 10980202] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
[5]"Putative capacitative calcium entry channels: expression of Drosophila trp and evidence for the existence of vertebrate homologues."
Petersen C.C.H., Berridge M.J., Borgese M.F., Bennett D.L.
Biochem. J. 311:41-44(1995) [PubMed: 7575478] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 505-642.
Tissue: Brain.
[6]"Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4-/- mice."
Freichel M., Suh S.H., Pfeifer A., Schweig U., Trost C., Weissgerber P., Biel M., Philipp S., Freise D., Droogmans G., Hofmann F., Flockerzi V., Nilius B.
Nat. Cell Biol. 3:121-127(2001) [PubMed: 11175743] [Abstract]
Cited for: FUNCTION.
[7]"The self-association of two N-terminal interaction domains plays an important role in the tetramerization of TRPC4."
Lepage P.K., Lussier M.P., McDuff F.O., Lavigne P., Boulay G.
Cell Calcium 45:251-259(2009) [PubMed: 19070363] [Abstract]
Cited for: SUBUNIT.
[8]"TRUSS, TNF-R1, and TRPC ion channels synergistically reverse endoplasmic reticulum Ca2+ storage reduction in response to m1 muscarinic acetylcholine receptor signaling."
Mace K.E., Lussier M.P., Boulay G., Terry-Powers J.L., Parfrey H., Perraud A.L., Riches D.W.H.
J. Cell. Physiol. 225:444-453(2010) [PubMed: 20458742] [Abstract]
Cited for: INTERACTION WITH TRPC4AP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF011543 mRNA. Translation: AAD10167.1.
U50922 mRNA. Translation: AAC05179.1.
AF190646 mRNA. Translation: AAF01469.1.
U50921 mRNA. Translation: AAC05178.1.
AF019663 mRNA. Translation: AAD10168.1.
X90697 mRNA. Translation: CAA62230.1.
IPIIPI00132045.
IPI00229745.
PIRS59128.
RefSeqNP_058680.1. NM_016984.2.
UniGeneMm.10100.

3D structure databases

ProteinModelPortalQ9QUQ5.
SMRQ9QUQ5. Positions 33-174.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-144626.
STRINGQ9QUQ5.

PTM databases

PhosphoSiteQ9QUQ5.

Proteomic databases

PRIDEQ9QUQ5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029311; ENSMUSP00000029311; ENSMUSG00000027748.
ENSMUST00000108005; ENSMUSP00000103640; ENSMUSG00000027748.
GeneID22066.
KEGGmmu:22066.
UCSCuc008pff.1. mouse.

Organism-specific databases

CTD7223.
MGIMGI:109525. Trpc4.

Phylogenomic databases

GeneTreeENSGT00550000074237.
HOGENOMHBG355804.
HOVERGENHBG068337.
InParanoidQ9QUQ5.
OMANMAQFYY.
OrthoDBEOG4V6ZG2.
PhylomeDBQ9QUQ5.

Gene expression databases

ArrayExpressQ9QUQ5.
BgeeQ9QUQ5.
GenevestigatorQ9QUQ5.
GermOnlineENSMUSG00000027748. Mus musculus.

Family and domain databases

InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans.
IPR013555. TRP_2.
IPR004729. TRP_channel.
IPR005460. TRPC4_channel.
IPR002153. TRPC_channel.
[Graphical view]
Gene3DG3DSA:1.25.40.20. ANK. 1 hit.
KOK04967.
PfamPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSPR01097. TRNSRECEPTRP.
PR01645. TRPCHANNEL4.
SMARTSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMSSF48403. ANK. 1 hit.
TIGRFAMsTIGR00870. Trp. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio301884.
SOURCESearch...

Entry information

Entry nameTRPC4_MOUSE
AccessionPrimary (citable) accession number: Q9QUQ5
Secondary accession number(s): Q62350, Q9QUQ9, Q9QZC0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: November 16, 2011
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents