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Protein

Short transient receptor potential channel 4

Gene

Trpc4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. Trpc4 deficient mice lack a store-operated calcium entry in endothelial cells.By similarity1 Publication

GO - Molecular functioni

  • beta-catenin binding Source: MGI
  • cadherin binding Source: MGI
  • inositol 1,4,5 trisphosphate binding Source: BHF-UCL
  • store-operated calcium channel activity Source: MGI

GO - Biological processi

  • calcium ion import Source: MGI
  • gamma-aminobutyric acid secretion Source: MGI
  • oligodendrocyte differentiation Source: MGI
  • regulation of calcium ion transport Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Short transient receptor potential channel 4
Short name:
TrpC4
Alternative name(s):
Capacitative calcium entry channel Trp4
Receptor-activated cation channel TRP4
Gene namesi
Name:Trpc4
Synonyms:Trrp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:109525. Trpc4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 329329CytoplasmicSequence analysisAdd
BLAST
Transmembranei330 – 35021HelicalSequence analysisAdd
BLAST
Topological domaini351 – 36212ExtracellularSequence analysisAdd
BLAST
Transmembranei363 – 38321HelicalSequence analysisAdd
BLAST
Topological domaini384 – 43653CytoplasmicSequence analysisAdd
BLAST
Transmembranei437 – 45721HelicalSequence analysisAdd
BLAST
Topological domaini458 – 46912ExtracellularSequence analysisAdd
BLAST
Transmembranei470 – 49021HelicalSequence analysisAdd
BLAST
Topological domaini491 – 51121CytoplasmicSequence analysisAdd
BLAST
Transmembranei512 – 53221HelicalSequence analysisAdd
BLAST
Topological domaini533 – 59967ExtracellularSequence analysisAdd
BLAST
Transmembranei600 – 62021HelicalSequence analysisAdd
BLAST
Topological domaini621 – 974354CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: MGI
  • calcium channel complex Source: MGI
  • caveola Source: MGI
  • cell-cell junction Source: Ensembl
  • cell surface Source: MGI
  • cortical cytoskeleton Source: MGI
  • integral component of plasma membrane Source: Ensembl
  • membrane raft Source: MGI
  • plasma membrane Source: BHF-UCL
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1741219.
GuidetoPHARMACOLOGYi489.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 974974Short transient receptor potential channel 4PRO_0000215315Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei956 – 9561Phosphotyrosine; by FYNBy similarity
Modified residuei969 – 9691Phosphotyrosine; by FYNBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9QUQ5.
PaxDbiQ9QUQ5.
PRIDEiQ9QUQ5.

PTM databases

PhosphoSiteiQ9QUQ5.

Expressioni

Tissue specificityi

Abundantly expressed in brain (hippocampal CA1 pyramidal neurons, dentate gyrus granule cells, and cerebral cortical neurons, and in the septal nuclei and the mitral layer of olfactory bulb). Lower levels are detected in other tissues.

Gene expression databases

BgeeiQ9QUQ5.
ExpressionAtlasiQ9QUQ5. baseline and differential.
GenevisibleiQ9QUQ5. MM.

Interactioni

Subunit structurei

Homotetramer. Heterotetramer with TRPC1 and/or TRPC5 (By similarity). Isoform alpha but not isoform beta associates with inositol 1,4,5-trisphosphate receptor (ITPR) (By similarity). Interacts with NHERF. Interacts with MX1 and RNF24 (By similarity). Interacts (via CIRB domain) with SESTD1 (via the spectrin 1 repeat) and SPTBN5 (via C-terminus) (By similarity). Interacts with CDH5 and CTNNB1 (By similarity). Interacts (via protein 4.1-binding domain) with EPB41L2 (By similarity). Interacts with TRPC4AP.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204330. 5 interactions.
DIPiDIP-40848N.
IntActiQ9QUQ5. 2 interactions.
MINTiMINT-144626.
STRINGi10090.ENSMUSP00000029311.

Chemistry

BindingDBiQ9QUQ5.

Structurei

3D structure databases

ProteinModelPortaliQ9QUQ5.
SMRiQ9QUQ5. Positions 97-172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati31 – 6030ANK 1Add
BLAST
Repeati69 – 9729ANK 2Add
BLAST
Repeati98 – 12427ANK 3Add
BLAST
Repeati141 – 17030ANK 4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 17286Multimerization domainAdd
BLAST
Regioni254 – 30451Multimerization domainAdd
BLAST
Regioni615 – 974360Binds to ITPR1, ITPR2 and ITPR3By similarityAdd
BLAST
Regioni972 – 9743PDZ-binding domainBy similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili223 – 26038Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 4 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3609. Eukaryota.
ENOG410XQ0Y. LUCA.
GeneTreeiENSGT00760000119180.
HOGENOMiHOG000020589.
HOVERGENiHBG068337.
InParanoidiQ9QUQ5.
KOiK04967.
OMAiCILVDHR.
OrthoDBiEOG72G17P.
PhylomeDBiQ9QUQ5.
TreeFamiTF313147.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR005460. TRPC4_channel.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERiPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF25. PTHR10117:SF25. 1 hit.
PfamiPF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSiPR01097. TRNSRECEPTRP.
PR01645. TRPCHANNEL4.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q9QUQ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQFYYKRNV NAPYRDRIPL RIVRAESELS PSEKAYLNAV EKGDYASVKK
60 70 80 90 100
SLEEAEIYFK ININCIDPLG RTALLIAIEN ENLELIELLL SFNVYVGDAL
110 120 130 140 150
LHAIRKEVVG AVELLLNHKK PSGEKQVPPI LLDKQFSEFT PDITPIILAA
160 170 180 190 200
HTNNYEIIKL LVQKGVSVPR PHEVRCNCVE CVSSSDVDSL RHSRSRLNIY
210 220 230 240 250
KALASPSLIA LSSEDPFLTA FQLSWELQEL SKVENEFKSE YEELSRQCKQ
260 270 280 290 300
FAKDLLDQTR SSRELEIILN YRDDNSLIEE QSGNDLARLK LAIKYRQKEF
310 320 330 340 350
VAQPNCQQLL ASRWYDEFPG WRRRHWAVKM VTCFIIGLLF PVFSVCYLIA
360 370 380 390 400
PKSPLGLFIR KPFIKFICHT ASYLTFLFLL LLASQHIDRS DLNRQGPPPT
410 420 430 440 450
IVEWMILPWV LGFIWGEIKQ MWDGGLQDYI HDWWNLMDFV MNSLYLATIS
460 470 480 490 500
LKIVAFVKYS ALNPRESWDM WHPTLVAEAL FAIANIFSSL RLISLFTANS
510 520 530 540 550
HLGPLQISLG RMLLDILKFL FIYCLVLLAF ANGLNQLYFY YEETKGLSCK
560 570 580 590 600
GIRCEKQNNA FSTLFETLQS LFWSIFGLIN LYVTNVKAQH EFTEFVGATM
610 620 630 640 650
FGTYNVISLV VLLNMLIAMM NNSYQLIADH ADIEWKFART KLWMSYFEEG
660 670 680 690 700
GTLPTPFNVI PSPKSLWYLV KWIWTHLCKK KMRRKPESFG TIGRRAADNL
710 720 730 740 750
RRHHQYQEVM RNLVKRYVAA MIREAKTEEG LTEENVKELK QDISSFRFEV
760 770 780 790 800
LGLLRGSKLS TIQSANAASS ADSDEKSQSE GNGKDKRKNL SLFDLTTLIH
810 820 830 840 850
PRSAAIASER HNLSNGSALV VQEPPREKQR KVNFVADIKN FGLFHRRSKQ
860 870 880 890 900
NAAEQNANQI FSVSEEITRQ QAAGALERNI ELESKGLASR GDRSIPGLNE
910 920 930 940 950
QCVLVDHRER NTDTLGLQVG KRVCSTFKSE KVVVEDTVPI IPKEKHAHEE
960 970
DSSIDYDLSP TDTAAHEDYV TTRL
Length:974
Mass (Da):111,575
Last modified:May 1, 2000 - v1
Checksum:i2D0BB2F235F5F8D1
GO
Isoform Beta (identifier: Q9QUQ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     781-864: Missing.

Show »
Length:890
Mass (Da):102,181
Checksum:i26901B5A81068694
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti780 – 7801E → K in AAF01469 (PubMed:9512398).Curated
Sequence conflicti890 – 8901R → L in AAF01469 (PubMed:9512398).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei781 – 86484Missing in isoform Beta. 4 PublicationsVSP_006570Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011543 mRNA. Translation: AAD10167.1.
U50922 mRNA. Translation: AAC05179.1.
AF190646 mRNA. Translation: AAF01469.1.
U50921 mRNA. Translation: AAC05178.1.
AF019663 mRNA. Translation: AAD10168.1.
X90697 mRNA. Translation: CAA62230.1.
CCDSiCCDS17350.1. [Q9QUQ5-1]
CCDS79904.1. [Q9QUQ5-2]
PIRiS59128.
RefSeqiNP_001240611.1. NM_001253682.1. [Q9QUQ5-2]
NP_058680.1. NM_016984.3. [Q9QUQ5-1]
XP_006501359.1. XM_006501296.2. [Q9QUQ5-1]
UniGeneiMm.10100.
Mm.489865.

Genome annotation databases

EnsembliENSMUST00000029311; ENSMUSP00000029311; ENSMUSG00000027748. [Q9QUQ5-1]
ENSMUST00000200048; ENSMUSP00000143593; ENSMUSG00000027748. [Q9QUQ5-2]
GeneIDi22066.
KEGGimmu:22066.
UCSCiuc008pfd.2. mouse. [Q9QUQ5-1]
uc008pff.2. mouse. [Q9QUQ5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011543 mRNA. Translation: AAD10167.1.
U50922 mRNA. Translation: AAC05179.1.
AF190646 mRNA. Translation: AAF01469.1.
U50921 mRNA. Translation: AAC05178.1.
AF019663 mRNA. Translation: AAD10168.1.
X90697 mRNA. Translation: CAA62230.1.
CCDSiCCDS17350.1. [Q9QUQ5-1]
CCDS79904.1. [Q9QUQ5-2]
PIRiS59128.
RefSeqiNP_001240611.1. NM_001253682.1. [Q9QUQ5-2]
NP_058680.1. NM_016984.3. [Q9QUQ5-1]
XP_006501359.1. XM_006501296.2. [Q9QUQ5-1]
UniGeneiMm.10100.
Mm.489865.

3D structure databases

ProteinModelPortaliQ9QUQ5.
SMRiQ9QUQ5. Positions 97-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204330. 5 interactions.
DIPiDIP-40848N.
IntActiQ9QUQ5. 2 interactions.
MINTiMINT-144626.
STRINGi10090.ENSMUSP00000029311.

Chemistry

BindingDBiQ9QUQ5.
ChEMBLiCHEMBL1741219.
GuidetoPHARMACOLOGYi489.

PTM databases

PhosphoSiteiQ9QUQ5.

Proteomic databases

MaxQBiQ9QUQ5.
PaxDbiQ9QUQ5.
PRIDEiQ9QUQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029311; ENSMUSP00000029311; ENSMUSG00000027748. [Q9QUQ5-1]
ENSMUST00000200048; ENSMUSP00000143593; ENSMUSG00000027748. [Q9QUQ5-2]
GeneIDi22066.
KEGGimmu:22066.
UCSCiuc008pfd.2. mouse. [Q9QUQ5-1]
uc008pff.2. mouse. [Q9QUQ5-2]

Organism-specific databases

CTDi7223.
MGIiMGI:109525. Trpc4.

Phylogenomic databases

eggNOGiKOG3609. Eukaryota.
ENOG410XQ0Y. LUCA.
GeneTreeiENSGT00760000119180.
HOGENOMiHOG000020589.
HOVERGENiHBG068337.
InParanoidiQ9QUQ5.
KOiK04967.
OMAiCILVDHR.
OrthoDBiEOG72G17P.
PhylomeDBiQ9QUQ5.
TreeFamiTF313147.

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.

Miscellaneous databases

NextBioi301884.
PROiQ9QUQ5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QUQ5.
ExpressionAtlasiQ9QUQ5. baseline and differential.
GenevisibleiQ9QUQ5. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR005460. TRPC4_channel.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERiPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF25. PTHR10117:SF25. 1 hit.
PfamiPF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSiPR01097. TRNSRECEPTRP.
PR01645. TRPCHANNEL4.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Trp4 is involved in capacitative calcium entry in murine cells."
    Zhu X., Boulay G., Jiang M., Birnbaumer L.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
    Tissue: Brain.
  2. Qian F., Philipson L.H.
    Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
  3. "Differential distribution of TRP Ca2+ channel isoforms in mouse brain."
    Mori Y., Takada N., Okada T., Wakamori M., Imoto K., Wanifuchi H., Oka H., Oba A., Ikenaka K., Kurosaki T.
    NeuroReport 9:507-515(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
    Tissue: Brain.
  4. "Association of mammalian trp4 and phospholipase C isozymes with a PDZ domain-containing protein, NHERF."
    Tang Y., Tang J., Chen Z., Trost C., Flockerzi V., Li M., Ramesh V., Zhu M.X.
    J. Biol. Chem. 275:37559-37564(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA).
  5. "Putative capacitative calcium entry channels: expression of Drosophila trp and evidence for the existence of vertebrate homologues."
    Petersen C.C.H., Berridge M.J., Borgese M.F., Bennett D.L.
    Biochem. J. 311:41-44(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 505-642.
    Tissue: Brain.
  6. "Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4-/- mice."
    Freichel M., Suh S.H., Pfeifer A., Schweig U., Trost C., Weissgerber P., Biel M., Philipp S., Freise D., Droogmans G., Hofmann F., Flockerzi V., Nilius B.
    Nat. Cell Biol. 3:121-127(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The self-association of two N-terminal interaction domains plays an important role in the tetramerization of TRPC4."
    Lepage P.K., Lussier M.P., McDuff F.O., Lavigne P., Boulay G.
    Cell Calcium 45:251-259(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  8. "TRUSS, TNF-R1, and TRPC ion channels synergistically reverse endoplasmic reticulum Ca2+ storage reduction in response to m1 muscarinic acetylcholine receptor signaling."
    Mace K.E., Lussier M.P., Boulay G., Terry-Powers J.L., Parfrey H., Perraud A.L., Riches D.W.H.
    J. Cell. Physiol. 225:444-453(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRPC4AP.

Entry informationi

Entry nameiTRPC4_MOUSE
AccessioniPrimary (citable) accession number: Q9QUQ5
Secondary accession number(s): Q62350, Q9QUQ9, Q9QZC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 148 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.