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Protein

Short transient receptor potential channel 4

Gene

Trpc4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. Trpc4 deficient mice lack a store-operated calcium entry in endothelial cells.By similarity1 Publication

GO - Molecular functioni

  • beta-catenin binding Source: MGI
  • cadherin binding Source: MGI
  • inositol 1,4,5 trisphosphate binding Source: BHF-UCL
  • store-operated calcium channel activity Source: MGI

GO - Biological processi

  • calcium ion import Source: MGI
  • gamma-aminobutyric acid secretion Source: MGI
  • manganese ion transport Source: GO_Central
  • oligodendrocyte differentiation Source: MGI
  • regulation of cytosolic calcium ion concentration Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Ion channel

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Short transient receptor potential channel 4
Short name:
TrpC4
Alternative name(s):
Capacitative calcium entry channel Trp4
Receptor-activated cation channel TRP4
Gene namesi
Name:Trpc4
Synonyms:Trrp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:109525. Trpc4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 329CytoplasmicSequence analysisAdd BLAST329
Transmembranei330 – 350HelicalSequence analysisAdd BLAST21
Topological domaini351 – 362ExtracellularSequence analysisAdd BLAST12
Transmembranei363 – 383HelicalSequence analysisAdd BLAST21
Topological domaini384 – 436CytoplasmicSequence analysisAdd BLAST53
Transmembranei437 – 457HelicalSequence analysisAdd BLAST21
Topological domaini458 – 469ExtracellularSequence analysisAdd BLAST12
Transmembranei470 – 490HelicalSequence analysisAdd BLAST21
Topological domaini491 – 511CytoplasmicSequence analysisAdd BLAST21
Transmembranei512 – 532HelicalSequence analysisAdd BLAST21
Topological domaini533 – 599ExtracellularSequence analysisAdd BLAST67
Transmembranei600 – 620HelicalSequence analysisAdd BLAST21
Topological domaini621 – 974CytoplasmicSequence analysisAdd BLAST354

GO - Cellular componenti

  • basolateral plasma membrane Source: MGI
  • calcium channel complex Source: MGI
  • caveola Source: MGI
  • cell-cell junction Source: Ensembl
  • cell surface Source: MGI
  • cortical cytoskeleton Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • membrane raft Source: MGI
  • plasma membrane Source: BHF-UCL
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1741219.
GuidetoPHARMACOLOGYi489.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153151 – 974Short transient receptor potential channel 4Add BLAST974

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei956Phosphotyrosine; by FYNBy similarity1
Modified residuei969Phosphotyrosine; by FYNBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QUQ5.
PRIDEiQ9QUQ5.

PTM databases

iPTMnetiQ9QUQ5.
PhosphoSitePlusiQ9QUQ5.

Expressioni

Tissue specificityi

Abundantly expressed in brain (hippocampal CA1 pyramidal neurons, dentate gyrus granule cells, and cerebral cortical neurons, and in the septal nuclei and the mitral layer of olfactory bulb). Lower levels are detected in other tissues.

Gene expression databases

BgeeiENSMUSG00000027748.
ExpressionAtlasiQ9QUQ5. baseline and differential.
GenevisibleiQ9QUQ5. MM.

Interactioni

Subunit structurei

Homotetramer. Heterotetramer with TRPC1 and/or TRPC5 (By similarity). Isoform alpha but not isoform beta associates with inositol 1,4,5-trisphosphate receptor (ITPR) (By similarity). Interacts with NHERF. Interacts with MX1 and RNF24 (By similarity). Interacts (via CIRB domain) with SESTD1 (via the spectrin 1 repeat) and SPTBN5 (via C-terminus) (By similarity). Interacts with CDH5 and CTNNB1 (By similarity). Interacts (via protein 4.1-binding domain) with EPB41L2 (By similarity). Interacts with TRPC4AP.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204330. 5 interactors.
DIPiDIP-40848N.
IntActiQ9QUQ5. 2 interactors.
MINTiMINT-144626.
STRINGi10090.ENSMUSP00000029311.

Chemistry databases

BindingDBiQ9QUQ5.

Structurei

3D structure databases

ProteinModelPortaliQ9QUQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati31 – 60ANK 1Add BLAST30
Repeati69 – 97ANK 2Add BLAST29
Repeati98 – 124ANK 3Add BLAST27
Repeati141 – 170ANK 4Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni87 – 172Multimerization domainAdd BLAST86
Regioni254 – 304Multimerization domainAdd BLAST51
Regioni615 – 974Binds to ITPR1, ITPR2 and ITPR3By similarityAdd BLAST360
Regioni972 – 974PDZ-binding domainBy similarity3

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili223 – 260Sequence analysisAdd BLAST38

Sequence similaritiesi

Contains 4 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3609. Eukaryota.
ENOG410XQ0Y. LUCA.
GeneTreeiENSGT00760000119180.
HOGENOMiHOG000020589.
HOVERGENiHBG068337.
InParanoidiQ9QUQ5.
KOiK04967.
OMAiCILVDHR.
OrthoDBiEOG091G029I.
PhylomeDBiQ9QUQ5.
TreeFamiTF313147.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR005460. TRPC4_channel.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERiPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF25. PTHR10117:SF25. 1 hit.
PfamiPF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSiPR01097. TRNSRECEPTRP.
PR01645. TRPCHANNEL4.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha (identifier: Q9QUQ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQFYYKRNV NAPYRDRIPL RIVRAESELS PSEKAYLNAV EKGDYASVKK
60 70 80 90 100
SLEEAEIYFK ININCIDPLG RTALLIAIEN ENLELIELLL SFNVYVGDAL
110 120 130 140 150
LHAIRKEVVG AVELLLNHKK PSGEKQVPPI LLDKQFSEFT PDITPIILAA
160 170 180 190 200
HTNNYEIIKL LVQKGVSVPR PHEVRCNCVE CVSSSDVDSL RHSRSRLNIY
210 220 230 240 250
KALASPSLIA LSSEDPFLTA FQLSWELQEL SKVENEFKSE YEELSRQCKQ
260 270 280 290 300
FAKDLLDQTR SSRELEIILN YRDDNSLIEE QSGNDLARLK LAIKYRQKEF
310 320 330 340 350
VAQPNCQQLL ASRWYDEFPG WRRRHWAVKM VTCFIIGLLF PVFSVCYLIA
360 370 380 390 400
PKSPLGLFIR KPFIKFICHT ASYLTFLFLL LLASQHIDRS DLNRQGPPPT
410 420 430 440 450
IVEWMILPWV LGFIWGEIKQ MWDGGLQDYI HDWWNLMDFV MNSLYLATIS
460 470 480 490 500
LKIVAFVKYS ALNPRESWDM WHPTLVAEAL FAIANIFSSL RLISLFTANS
510 520 530 540 550
HLGPLQISLG RMLLDILKFL FIYCLVLLAF ANGLNQLYFY YEETKGLSCK
560 570 580 590 600
GIRCEKQNNA FSTLFETLQS LFWSIFGLIN LYVTNVKAQH EFTEFVGATM
610 620 630 640 650
FGTYNVISLV VLLNMLIAMM NNSYQLIADH ADIEWKFART KLWMSYFEEG
660 670 680 690 700
GTLPTPFNVI PSPKSLWYLV KWIWTHLCKK KMRRKPESFG TIGRRAADNL
710 720 730 740 750
RRHHQYQEVM RNLVKRYVAA MIREAKTEEG LTEENVKELK QDISSFRFEV
760 770 780 790 800
LGLLRGSKLS TIQSANAASS ADSDEKSQSE GNGKDKRKNL SLFDLTTLIH
810 820 830 840 850
PRSAAIASER HNLSNGSALV VQEPPREKQR KVNFVADIKN FGLFHRRSKQ
860 870 880 890 900
NAAEQNANQI FSVSEEITRQ QAAGALERNI ELESKGLASR GDRSIPGLNE
910 920 930 940 950
QCVLVDHRER NTDTLGLQVG KRVCSTFKSE KVVVEDTVPI IPKEKHAHEE
960 970
DSSIDYDLSP TDTAAHEDYV TTRL
Length:974
Mass (Da):111,575
Last modified:May 1, 2000 - v1
Checksum:i2D0BB2F235F5F8D1
GO
Isoform Beta (identifier: Q9QUQ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     781-864: Missing.

Show »
Length:890
Mass (Da):102,181
Checksum:i26901B5A81068694
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti780E → K in AAF01469 (PubMed:9512398).Curated1
Sequence conflicti890R → L in AAF01469 (PubMed:9512398).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006570781 – 864Missing in isoform Beta. 4 PublicationsAdd BLAST84

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011543 mRNA. Translation: AAD10167.1.
U50922 mRNA. Translation: AAC05179.1.
AF190646 mRNA. Translation: AAF01469.1.
U50921 mRNA. Translation: AAC05178.1.
AF019663 mRNA. Translation: AAD10168.1.
X90697 mRNA. Translation: CAA62230.1.
CCDSiCCDS17350.1. [Q9QUQ5-1]
CCDS79904.1. [Q9QUQ5-2]
PIRiS59128.
RefSeqiNP_001240611.1. NM_001253682.1. [Q9QUQ5-2]
NP_058680.1. NM_016984.3. [Q9QUQ5-1]
XP_006501359.1. XM_006501296.3. [Q9QUQ5-1]
UniGeneiMm.10100.
Mm.489865.

Genome annotation databases

EnsembliENSMUST00000029311; ENSMUSP00000029311; ENSMUSG00000027748. [Q9QUQ5-1]
ENSMUST00000200048; ENSMUSP00000143593; ENSMUSG00000027748. [Q9QUQ5-2]
GeneIDi22066.
KEGGimmu:22066.
UCSCiuc008pfd.2. mouse. [Q9QUQ5-1]
uc008pff.2. mouse. [Q9QUQ5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011543 mRNA. Translation: AAD10167.1.
U50922 mRNA. Translation: AAC05179.1.
AF190646 mRNA. Translation: AAF01469.1.
U50921 mRNA. Translation: AAC05178.1.
AF019663 mRNA. Translation: AAD10168.1.
X90697 mRNA. Translation: CAA62230.1.
CCDSiCCDS17350.1. [Q9QUQ5-1]
CCDS79904.1. [Q9QUQ5-2]
PIRiS59128.
RefSeqiNP_001240611.1. NM_001253682.1. [Q9QUQ5-2]
NP_058680.1. NM_016984.3. [Q9QUQ5-1]
XP_006501359.1. XM_006501296.3. [Q9QUQ5-1]
UniGeneiMm.10100.
Mm.489865.

3D structure databases

ProteinModelPortaliQ9QUQ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204330. 5 interactors.
DIPiDIP-40848N.
IntActiQ9QUQ5. 2 interactors.
MINTiMINT-144626.
STRINGi10090.ENSMUSP00000029311.

Chemistry databases

BindingDBiQ9QUQ5.
ChEMBLiCHEMBL1741219.
GuidetoPHARMACOLOGYi489.

PTM databases

iPTMnetiQ9QUQ5.
PhosphoSitePlusiQ9QUQ5.

Proteomic databases

PaxDbiQ9QUQ5.
PRIDEiQ9QUQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029311; ENSMUSP00000029311; ENSMUSG00000027748. [Q9QUQ5-1]
ENSMUST00000200048; ENSMUSP00000143593; ENSMUSG00000027748. [Q9QUQ5-2]
GeneIDi22066.
KEGGimmu:22066.
UCSCiuc008pfd.2. mouse. [Q9QUQ5-1]
uc008pff.2. mouse. [Q9QUQ5-2]

Organism-specific databases

CTDi7223.
MGIiMGI:109525. Trpc4.

Phylogenomic databases

eggNOGiKOG3609. Eukaryota.
ENOG410XQ0Y. LUCA.
GeneTreeiENSGT00760000119180.
HOGENOMiHOG000020589.
HOVERGENiHBG068337.
InParanoidiQ9QUQ5.
KOiK04967.
OMAiCILVDHR.
OrthoDBiEOG091G029I.
PhylomeDBiQ9QUQ5.
TreeFamiTF313147.

Enzyme and pathway databases

ReactomeiR-MMU-3295583. TRP channels.

Miscellaneous databases

PROiQ9QUQ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027748.
ExpressionAtlasiQ9QUQ5. baseline and differential.
GenevisibleiQ9QUQ5. MM.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR005460. TRPC4_channel.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERiPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF25. PTHR10117:SF25. 1 hit.
PfamiPF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSiPR01097. TRNSRECEPTRP.
PR01645. TRPCHANNEL4.
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
TIGRFAMsiTIGR00870. trp. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPC4_MOUSE
AccessioniPrimary (citable) accession number: Q9QUQ5
Secondary accession number(s): Q62350, Q9QUQ9, Q9QZC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.