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Q9QUP4

- CHST5_MOUSE

UniProt

Q9QUP4 - CHST5_MOUSE

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Protein
Carbohydrate sulfotransferase 5
Gene
Chst5, Gst4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. May also have activity toward O-linked sugars of mucin-type acceptors.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 557PAPS By similarity
Nucleotide bindingi202 – 2109PAPS By similarity

GO - Molecular functioni

  1. N-acetylglucosamine 6-O-sulfotransferase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. N-acetylglucosamine metabolic process Source: UniProtKB
  2. carbohydrate metabolic process Source: UniProtKB-KW
  3. keratan sulfate biosynthetic process Source: UniProtKB
  4. sulfur compound metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiREACT_198578. Keratan sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 5 (EC:2.8.2.-)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4
Short name:
GST4
Intestinal N-acetylglucosamine-6-O-sulfotransferase
Short name:
I-GlcNAc6ST
Short name:
Intestinal GlcNAc-6-sulfotransferase
Short name:
mIGn6ST
N-acetylglucosamine 6-O-sulfotransferase 3
Short name:
GlcNAc6ST-3
Short name:
Gn6st-3
Gene namesi
Name:Chst5
Synonyms:Gst4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1931825. Chst5.

Subcellular locationi

Golgi apparatus membrane; Single-pass type II membrane protein By similarity
Note: Golgi membrane, early secretory pathway By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77Cytoplasmic Reviewed prediction
Transmembranei8 – 2619Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini27 – 395369Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Carbohydrate sulfotransferase 5
PRO_0000085196Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi116 – 1161N-linked (GlcNAc...) Reviewed prediction
Glycosylationi142 – 1421N-linked (GlcNAc...) Reviewed prediction
Glycosylationi229 – 2291N-linked (GlcNAc...) Reviewed prediction
Glycosylationi305 – 3051N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9QUP4.

PTM databases

PhosphoSiteiQ9QUP4.

Expressioni

Tissue specificityi

Expressed in cornea.1 Publication

Gene expression databases

BgeeiQ9QUP4.
GenevestigatoriQ9QUP4.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034430.

Structurei

3D structure databases

ProteinModelPortaliQ9QUP4.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300333.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG050949.
InParanoidiQ9QUP4.
KOiK09671.
OMAiFPRGAIS.
OrthoDBiEOG7RZ5S0.
PhylomeDBiQ9QUP4.
TreeFamiTF342871.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9QUP4-1 [UniParc]FASTAAdd to Basket

« Hide

MRLPRFSSTV MLSLLMVQTG ILVFLVSRQV PSSPAGLGER VHVLVLSSWR    50
SGSSFVGQLF SQHPDVFYLM EPAWHVWDTL SQGSAPALHM AVRDLIRSVF 100
LCDMDVFDAY LPWRRNISDL FQWAVSRALC SPPVCEAFAR GNISSEEVCK 150
PLCATRPFGL AQEACSSYSH VVLKEVRFFN LQVLYPLLSD PALNLRIVHL 200
VRDPRAVLRS REQTAKALAR DNGIVLGTNG TWVEADPRLR VVNEVCRSHV 250
RIAEAALHKP PPFLQDRYRL VRYEDLARDP LTVIRELYAF TGLGLTPQLQ 300
TWIHNITHGS GPGARREAFK TTSRDALSVS QAWRHTLPFA KIRRVQELCG 350
GALQLLGYRS VHSELEQRDL SLDLLLPRGM DSFKWASSTE KQPES 395
Length:395
Mass (Da):44,537
Last modified:May 1, 2000 - v1
Checksum:i3FDF71E43ED383BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF176840 mRNA. Translation: AAD56002.1.
AF176841 Genomic DNA. Translation: AAD56003.1.
CCDSiCCDS22682.1.
RefSeqiNP_064334.1. NM_019950.2.
UniGeneiMm.432506.

Genome annotation databases

EnsembliENSMUST00000034430; ENSMUSP00000034430; ENSMUSG00000031952.
GeneIDi56773.
KEGGimmu:56773.
UCSCiuc009nmz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF176840 mRNA. Translation: AAD56002.1 .
AF176841 Genomic DNA. Translation: AAD56003.1 .
CCDSi CCDS22682.1.
RefSeqi NP_064334.1. NM_019950.2.
UniGenei Mm.432506.

3D structure databases

ProteinModelPortali Q9QUP4.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000034430.

PTM databases

PhosphoSitei Q9QUP4.

Proteomic databases

PRIDEi Q9QUP4.

Protocols and materials databases

DNASUi 56773.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034430 ; ENSMUSP00000034430 ; ENSMUSG00000031952 .
GeneIDi 56773.
KEGGi mmu:56773.
UCSCi uc009nmz.1. mouse.

Organism-specific databases

CTDi 23563.
MGIi MGI:1931825. Chst5.

Phylogenomic databases

eggNOGi NOG300333.
GeneTreei ENSGT00530000062902.
HOGENOMi HOG000261614.
HOVERGENi HBG050949.
InParanoidi Q9QUP4.
KOi K09671.
OMAi FPRGAIS.
OrthoDBi EOG7RZ5S0.
PhylomeDBi Q9QUP4.
TreeFami TF342871.

Enzyme and pathway databases

Reactomei REACT_198578. Keratan sulfate biosynthesis.

Miscellaneous databases

NextBioi 313302.
PROi Q9QUP4.
SOURCEi Search...

Gene expression databases

Bgeei Q9QUP4.
Genevestigatori Q9QUP4.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view ]
Pfami PF00685. Sulfotransfer_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMi SSF52540. SSF52540. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue."
    Lee J.K., Bhakta S., Rosen S.D., Hemmerich S.
    Biochem. Biophys. Res. Commun. 263:543-549(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: C57BL/6.
    Tissue: Intestine.
  2. "Human corneal GlcNAc 6-O-sulfotransferase and mouse intestinal GlcNAc 6-O-sulfotransferase both produce keratan sulfate."
    Akama T.O., Nakayama J., Nishida K., Hiraoka N., Suzuki M., McAuliffe J., Hindsgaul O., Fukuda M., Fukuda M.N.
    J. Biol. Chem. 276:16271-16278(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBSTRATE SPECIFICITY.
  3. "Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate."
    Akama T.O., Misra A.K., Hindsgaul O., Fukuda M.N.
    J. Biol. Chem. 277:42505-42513(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBSTRATE SPECIFICITY.

Entry informationi

Entry nameiCHST5_MOUSE
AccessioniPrimary (citable) accession number: Q9QUP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: May 1, 2000
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In human, there are 2 related proteins, CHST5 and CHST6, the latter mediating sulfation of keratan in cornea. In mouse however, there is no CHST6 protein, CHST5 functioning as a corneal keratan sulfotransferase and mediates such function.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi