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Protein

Carbohydrate sulfotransferase 5

Gene

Chst5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. May also have activity toward O-linked sugars of mucin-type acceptors.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 557PAPSBy similarity
Nucleotide bindingi202 – 2109PAPSBy similarity

GO - Molecular functioni

  1. N-acetylglucosamine 6-O-sulfotransferase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. keratan sulfate biosynthetic process Source: UniProtKB
  3. N-acetylglucosamine metabolic process Source: UniProtKB
  4. sulfur compound metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiREACT_346228. Keratan sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 5 (EC:2.8.2.-)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4
Short name:
GST4
Intestinal N-acetylglucosamine-6-O-sulfotransferase
Short name:
I-GlcNAc6ST
Short name:
Intestinal GlcNAc-6-sulfotransferase
Short name:
mIGn6ST
N-acetylglucosamine 6-O-sulfotransferase 3
Short name:
GlcNAc6ST-3
Short name:
Gn6st-3
Gene namesi
Name:Chst5
Synonyms:Gst4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1931825. Chst5.

Subcellular locationi

Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
Note: Golgi membrane, early secretory pathway.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2619Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini27 – 395369LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Carbohydrate sulfotransferase 5PRO_0000085196Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9QUP4.
PRIDEiQ9QUP4.

PTM databases

PhosphoSiteiQ9QUP4.

Expressioni

Tissue specificityi

Expressed in cornea.1 Publication

Gene expression databases

BgeeiQ9QUP4.
GenevestigatoriQ9QUP4.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034430.

Structurei

3D structure databases

ProteinModelPortaliQ9QUP4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300333.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG050949.
InParanoidiQ9QUP4.
KOiK09671.
OMAiFPRGAIS.
OrthoDBiEOG7RZ5S0.
PhylomeDBiQ9QUP4.
TreeFamiTF342871.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9QUP4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLPRFSSTV MLSLLMVQTG ILVFLVSRQV PSSPAGLGER VHVLVLSSWR
60 70 80 90 100
SGSSFVGQLF SQHPDVFYLM EPAWHVWDTL SQGSAPALHM AVRDLIRSVF
110 120 130 140 150
LCDMDVFDAY LPWRRNISDL FQWAVSRALC SPPVCEAFAR GNISSEEVCK
160 170 180 190 200
PLCATRPFGL AQEACSSYSH VVLKEVRFFN LQVLYPLLSD PALNLRIVHL
210 220 230 240 250
VRDPRAVLRS REQTAKALAR DNGIVLGTNG TWVEADPRLR VVNEVCRSHV
260 270 280 290 300
RIAEAALHKP PPFLQDRYRL VRYEDLARDP LTVIRELYAF TGLGLTPQLQ
310 320 330 340 350
TWIHNITHGS GPGARREAFK TTSRDALSVS QAWRHTLPFA KIRRVQELCG
360 370 380 390
GALQLLGYRS VHSELEQRDL SLDLLLPRGM DSFKWASSTE KQPES
Length:395
Mass (Da):44,537
Last modified:April 30, 2000 - v1
Checksum:i3FDF71E43ED383BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176840 mRNA. Translation: AAD56002.1.
AF176841 Genomic DNA. Translation: AAD56003.1.
CCDSiCCDS22682.1.
RefSeqiNP_064334.1. NM_019950.2.
UniGeneiMm.432506.

Genome annotation databases

EnsembliENSMUST00000034430; ENSMUSP00000034430; ENSMUSG00000031952.
GeneIDi56773.
KEGGimmu:56773.
UCSCiuc009nmz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176840 mRNA. Translation: AAD56002.1.
AF176841 Genomic DNA. Translation: AAD56003.1.
CCDSiCCDS22682.1.
RefSeqiNP_064334.1. NM_019950.2.
UniGeneiMm.432506.

3D structure databases

ProteinModelPortaliQ9QUP4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034430.

PTM databases

PhosphoSiteiQ9QUP4.

Proteomic databases

MaxQBiQ9QUP4.
PRIDEiQ9QUP4.

Protocols and materials databases

DNASUi56773.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034430; ENSMUSP00000034430; ENSMUSG00000031952.
GeneIDi56773.
KEGGimmu:56773.
UCSCiuc009nmz.1. mouse.

Organism-specific databases

CTDi23563.
MGIiMGI:1931825. Chst5.

Phylogenomic databases

eggNOGiNOG300333.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG050949.
InParanoidiQ9QUP4.
KOiK09671.
OMAiFPRGAIS.
OrthoDBiEOG7RZ5S0.
PhylomeDBiQ9QUP4.
TreeFamiTF342871.

Enzyme and pathway databases

ReactomeiREACT_346228. Keratan sulfate biosynthesis.

Miscellaneous databases

NextBioi313302.
PROiQ9QUP4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QUP4.
GenevestigatoriQ9QUP4.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue."
    Lee J.K., Bhakta S., Rosen S.D., Hemmerich S.
    Biochem. Biophys. Res. Commun. 263:543-549(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: C57BL/6.
    Tissue: Intestine.
  2. "Human corneal GlcNAc 6-O-sulfotransferase and mouse intestinal GlcNAc 6-O-sulfotransferase both produce keratan sulfate."
    Akama T.O., Nakayama J., Nishida K., Hiraoka N., Suzuki M., McAuliffe J., Hindsgaul O., Fukuda M., Fukuda M.N.
    J. Biol. Chem. 276:16271-16278(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, SUBSTRATE SPECIFICITY.
  3. "Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate."
    Akama T.O., Misra A.K., Hindsgaul O., Fukuda M.N.
    J. Biol. Chem. 277:42505-42513(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBSTRATE SPECIFICITY.

Entry informationi

Entry nameiCHST5_MOUSE
AccessioniPrimary (citable) accession number: Q9QUP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 14, 2005
Last sequence update: April 30, 2000
Last modified: March 31, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In human, there are 2 related proteins, CHST5 and CHST6, the latter mediating sulfation of keratan in cornea. In mouse however, there is no CHST6 protein, CHST5 functioning as a corneal keratan sulfotransferase and mediates such function.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.