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Q9QUP4 (CHST5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carbohydrate sulfotransferase 5

EC=2.8.2.-
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4
Short name=GST4
Intestinal N-acetylglucosamine-6-O-sulfotransferase
Short name=I-GlcNAc6ST
Short name=Intestinal GlcNAc-6-sulfotransferase
Short name=mIGn6ST
N-acetylglucosamine 6-O-sulfotransferase 3
Short name=GlcNAc6ST-3
Short name=Gn6st-3
Gene names
Name:Chst5
Synonyms:Gst4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. May also have activity toward O-linked sugars of mucin-type acceptors. Ref.3

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Note: Golgi membrane, early secretory pathway By similarity.

Tissue specificity

Expressed in cornea. Ref.2

Miscellaneous

In human, there are 2 related proteins, CHST5 and CHST6, the latter mediating sulfation of keratan in cornea. In mouse however, there is no CHST6 protein, CHST5 functioning as a corneal keratan sulfotransferase and mediates such function.

Sequence similarities

Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 395395Carbohydrate sulfotransferase 5
PRO_0000085196

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 2619Helical; Signal-anchor for type II membrane protein; Potential
Topological domain27 – 395369Lumenal Potential
Nucleotide binding49 – 557PAPS By similarity
Nucleotide binding202 – 2109PAPS By similarity

Amino acid modifications

Glycosylation1161N-linked (GlcNAc...) Potential
Glycosylation1421N-linked (GlcNAc...) Potential
Glycosylation2291N-linked (GlcNAc...) Potential
Glycosylation3051N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9QUP4 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 3FDF71E43ED383BE

FASTA39544,537
        10         20         30         40         50         60 
MRLPRFSSTV MLSLLMVQTG ILVFLVSRQV PSSPAGLGER VHVLVLSSWR SGSSFVGQLF 

        70         80         90        100        110        120 
SQHPDVFYLM EPAWHVWDTL SQGSAPALHM AVRDLIRSVF LCDMDVFDAY LPWRRNISDL 

       130        140        150        160        170        180 
FQWAVSRALC SPPVCEAFAR GNISSEEVCK PLCATRPFGL AQEACSSYSH VVLKEVRFFN 

       190        200        210        220        230        240 
LQVLYPLLSD PALNLRIVHL VRDPRAVLRS REQTAKALAR DNGIVLGTNG TWVEADPRLR 

       250        260        270        280        290        300 
VVNEVCRSHV RIAEAALHKP PPFLQDRYRL VRYEDLARDP LTVIRELYAF TGLGLTPQLQ 

       310        320        330        340        350        360 
TWIHNITHGS GPGARREAFK TTSRDALSVS QAWRHTLPFA KIRRVQELCG GALQLLGYRS 

       370        380        390 
VHSELEQRDL SLDLLLPRGM DSFKWASSTE KQPES 

« Hide

References

[1]"Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue."
Lee J.K., Bhakta S., Rosen S.D., Hemmerich S.
Biochem. Biophys. Res. Commun. 263:543-549(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: C57BL/6.
Tissue: Intestine.
[2]"Human corneal GlcNAc 6-O-sulfotransferase and mouse intestinal GlcNAc 6-O-sulfotransferase both produce keratan sulfate."
Akama T.O., Nakayama J., Nishida K., Hiraoka N., Suzuki M., McAuliffe J., Hindsgaul O., Fukuda M., Fukuda M.N.
J. Biol. Chem. 276:16271-16278(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, SUBSTRATE SPECIFICITY.
[3]"Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate."
Akama T.O., Misra A.K., Hindsgaul O., Fukuda M.N.
J. Biol. Chem. 277:42505-42513(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBSTRATE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF176840 mRNA. Translation: AAD56002.1.
AF176841 Genomic DNA. Translation: AAD56003.1.
CCDSCCDS22682.1.
RefSeqNP_064334.1. NM_019950.2.
UniGeneMm.432506.

3D structure databases

ProteinModelPortalQ9QUP4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000034430.

PTM databases

PhosphoSiteQ9QUP4.

Proteomic databases

PRIDEQ9QUP4.

Protocols and materials databases

DNASU56773.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034430; ENSMUSP00000034430; ENSMUSG00000031952.
GeneID56773.
KEGGmmu:56773.
UCSCuc009nmz.1. mouse.

Organism-specific databases

CTD23563.
MGIMGI:1931825. Chst5.

Phylogenomic databases

eggNOGNOG300333.
GeneTreeENSGT00530000062902.
HOGENOMHOG000261614.
HOVERGENHBG050949.
InParanoidQ9QUP4.
KOK09671.
OMAFPRGAIS.
OrthoDBEOG7RZ5S0.
PhylomeDBQ9QUP4.
TreeFamTF342871.

Gene expression databases

BgeeQ9QUP4.
GenevestigatorQ9QUP4.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMSSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

NextBio313302.
PROQ9QUP4.
SOURCESearch...

Entry information

Entry nameCHST5_MOUSE
AccessionPrimary (citable) accession number: Q9QUP4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot