Q9QUP4 (CHST5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbohydrate sulfotransferase 5
EC=2.8.2.-

Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4
Short name=GST4
Intestinal N-acetylglucosamine-6-O-sulfotransferase
Short name=I-GlcNAc6ST
Short name=Intestinal GlcNAc-6-sulfotransferase
Short name=mIGn6ST
N-acetylglucosamine 6-O-sulfotransferase 3
Short name=GlcNAc6ST-3
Short name=Gn6st-3

Gene names

Name:	Chst5
Synonyms:	Gst4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. May also have activity toward O-linked sugars of mucin-type acceptors. Ref.3
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Note: Golgi membrane, early secretory pathway By similarity.
Tissue specificity	Expressed in cornea. Ref.2
Miscellaneous	In human, there are 2 related proteins, CHST5 and CHST6, the latter mediating sulfation of keratan in cornea. In mouse however, there is no CHST6 protein, CHST5 functioning as a corneal keratan sulfotransferase and mediates such function.
Sequence similarities	Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-acetylglucosamine metabolic process Inferred from direct assay Ref.2Ref.3. Source: UniProtKB carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW keratan sulfate biosynthetic process Inferred from direct assay Ref.2. Source: UniProtKB
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	N-acetylglucosamine 6-O-sulfotransferase activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Carbohydrate sulfotransferase 5		PRO_0000085196
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 26	19	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	27 – 395	369	Lumenal Potential
	Nucleotide binding	49 – 55	7	PAPS By similarity
	Nucleotide binding	202 – 210	9	PAPS By similarity
Amino acid modifications
	Glycosylation	116	1	N-linked (GlcNAc...) Potential
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	229	1	N-linked (GlcNAc...) Potential
	Glycosylation	305	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9QUP4 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 3FDF71E43ED383BE	FASTA	395	44,537
	10 20 30 40 50 60 MRLPRFSSTV MLSLLMVQTG ILVFLVSRQV PSSPAGLGER VHVLVLSSWR SGSSFVGQLF 70 80 90 100 110 120 SQHPDVFYLM EPAWHVWDTL SQGSAPALHM AVRDLIRSVF LCDMDVFDAY LPWRRNISDL 130 140 150 160 170 180 FQWAVSRALC SPPVCEAFAR GNISSEEVCK PLCATRPFGL AQEACSSYSH VVLKEVRFFN 190 200 210 220 230 240 LQVLYPLLSD PALNLRIVHL VRDPRAVLRS REQTAKALAR DNGIVLGTNG TWVEADPRLR 250 260 270 280 290 300 VVNEVCRSHV RIAEAALHKP PPFLQDRYRL VRYEDLARDP LTVIRELYAF TGLGLTPQLQ 310 320 330 340 350 360 TWIHNITHGS GPGARREAFK TTSRDALSVS QAWRHTLPFA KIRRVQELCG GALQLLGYRS 370 380 390 VHSELEQRDL SLDLLLPRGM DSFKWASSTE KQPES

References

[1]	"Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue." Lee J.K., Bhakta S., Rosen S.D., Hemmerich S. Biochem. Biophys. Res. Commun. 263:543-549(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: C57BL/6. Tissue: Intestine.
[2]	"Human corneal GlcNAc 6-O-sulfotransferase and mouse intestinal GlcNAc 6-O-sulfotransferase both produce keratan sulfate." Akama T.O., Nakayama J., Nishida K., Hiraoka N., Suzuki M., McAuliffe J., Hindsgaul O., Fukuda M., Fukuda M.N. J. Biol. Chem. 276:16271-16278(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, SUBSTRATE SPECIFICITY.
[3]	"Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate." Akama T.O., Misra A.K., Hindsgaul O., Fukuda M.N. J. Biol. Chem. 277:42505-42513(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBSTRATE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF176840 mRNA. Translation: AAD56002.1. AF176841 Genomic DNA. Translation: AAD56003.1.
IPI	IPI00131990.
RefSeq	NP_064334.1. NM_019950.2.
UniGene	Mm.432506.
3D structure databases
ProteinModelPortal	Q9QUP4.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000034430.
PTM databases
PhosphoSite	Q9QUP4.
Proteomic databases
PRIDE	Q9QUP4.
Protocols and materials databases
DNASU	56773.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034430; ENSMUSP00000034430; ENSMUSG00000031952.
GeneID	56773.
KEGG	mmu:56773.
UCSC	uc009nmz.1. mouse.
Organism-specific databases
CTD	23563.
MGI	MGI:1931825. Chst5.
Phylogenomic databases
eggNOG	NOG300333.
GeneTree	ENSGT00530000062902.
HOGENOM	HOG000261614.
HOVERGEN	HBG050949.
InParanoid	Q9QUP4.
KO	K09671.
OMA	FSWASST.
OrthoDB	EOG4RXZ09.
Gene expression databases
Bgee	Q9QUP4.
Genevestigator	Q9QUP4.
GermOnline	ENSMUSG00000031952. Mus musculus.
Family and domain databases
InterPro	IPR016469. Carbohydrate_sulfotransferase. IPR000863. Sulfotransferase_dom. [Graphical view]
Pfam	PF00685. Sulfotransfer_1. 1 hit. [Graphical view]
PIRSF	PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
ProtoNet	Search...
Other
NextBio	313302.
SOURCE	Search...

Entry information

Entry name

CHST5_MOUSE

Accession

Primary (citable) accession number: Q9QUP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program