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Q9QUP4

- CHST5_MOUSE

UniProt

Q9QUP4 - CHST5_MOUSE

Protein

Carbohydrate sulfotransferase 5

Gene

Chst5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues of keratan. Mediates sulfation of keratan in cornea. Keratan sulfate plays a central role in maintaining corneal transparency. Acts on the non-reducing terminal GlcNAc of short and long carbohydrate substrates that have poly-N-acetyllactosamine structures. May also have activity toward O-linked sugars of mucin-type acceptors.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi49 – 557PAPSBy similarity
    Nucleotide bindingi202 – 2109PAPSBy similarity

    GO - Molecular functioni

    1. N-acetylglucosamine 6-O-sulfotransferase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. keratan sulfate biosynthetic process Source: UniProtKB
    3. N-acetylglucosamine metabolic process Source: UniProtKB
    4. sulfur compound metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Enzyme and pathway databases

    ReactomeiREACT_198578. Keratan sulfate biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbohydrate sulfotransferase 5 (EC:2.8.2.-)
    Alternative name(s):
    Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4
    Short name:
    GST4
    Intestinal N-acetylglucosamine-6-O-sulfotransferase
    Short name:
    I-GlcNAc6ST
    Short name:
    Intestinal GlcNAc-6-sulfotransferase
    Short name:
    mIGn6ST
    N-acetylglucosamine 6-O-sulfotransferase 3
    Short name:
    GlcNAc6ST-3
    Short name:
    Gn6st-3
    Gene namesi
    Name:Chst5
    Synonyms:Gst4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:1931825. Chst5.

    Subcellular locationi

    Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Golgi membrane, early secretory pathway.By similarity

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 395395Carbohydrate sulfotransferase 5PRO_0000085196Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi229 – 2291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ9QUP4.

    PTM databases

    PhosphoSiteiQ9QUP4.

    Expressioni

    Tissue specificityi

    Expressed in cornea.1 Publication

    Gene expression databases

    BgeeiQ9QUP4.
    GenevestigatoriQ9QUP4.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000034430.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QUP4.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77CytoplasmicSequence Analysis
    Topological domaini27 – 395369LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2619Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300333.
    GeneTreeiENSGT00530000062902.
    HOGENOMiHOG000261614.
    HOVERGENiHBG050949.
    InParanoidiQ9QUP4.
    KOiK09671.
    OMAiFPRGAIS.
    OrthoDBiEOG7RZ5S0.
    PhylomeDBiQ9QUP4.
    TreeFamiTF342871.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view]
    PfamiPF00685. Sulfotransfer_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMiSSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q9QUP4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLPRFSSTV MLSLLMVQTG ILVFLVSRQV PSSPAGLGER VHVLVLSSWR    50
    SGSSFVGQLF SQHPDVFYLM EPAWHVWDTL SQGSAPALHM AVRDLIRSVF 100
    LCDMDVFDAY LPWRRNISDL FQWAVSRALC SPPVCEAFAR GNISSEEVCK 150
    PLCATRPFGL AQEACSSYSH VVLKEVRFFN LQVLYPLLSD PALNLRIVHL 200
    VRDPRAVLRS REQTAKALAR DNGIVLGTNG TWVEADPRLR VVNEVCRSHV 250
    RIAEAALHKP PPFLQDRYRL VRYEDLARDP LTVIRELYAF TGLGLTPQLQ 300
    TWIHNITHGS GPGARREAFK TTSRDALSVS QAWRHTLPFA KIRRVQELCG 350
    GALQLLGYRS VHSELEQRDL SLDLLLPRGM DSFKWASSTE KQPES 395
    Length:395
    Mass (Da):44,537
    Last modified:May 1, 2000 - v1
    Checksum:i3FDF71E43ED383BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF176840 mRNA. Translation: AAD56002.1.
    AF176841 Genomic DNA. Translation: AAD56003.1.
    CCDSiCCDS22682.1.
    RefSeqiNP_064334.1. NM_019950.2.
    UniGeneiMm.432506.

    Genome annotation databases

    EnsembliENSMUST00000034430; ENSMUSP00000034430; ENSMUSG00000031952.
    GeneIDi56773.
    KEGGimmu:56773.
    UCSCiuc009nmz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF176840 mRNA. Translation: AAD56002.1 .
    AF176841 Genomic DNA. Translation: AAD56003.1 .
    CCDSi CCDS22682.1.
    RefSeqi NP_064334.1. NM_019950.2.
    UniGenei Mm.432506.

    3D structure databases

    ProteinModelPortali Q9QUP4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000034430.

    PTM databases

    PhosphoSitei Q9QUP4.

    Proteomic databases

    PRIDEi Q9QUP4.

    Protocols and materials databases

    DNASUi 56773.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034430 ; ENSMUSP00000034430 ; ENSMUSG00000031952 .
    GeneIDi 56773.
    KEGGi mmu:56773.
    UCSCi uc009nmz.1. mouse.

    Organism-specific databases

    CTDi 23563.
    MGIi MGI:1931825. Chst5.

    Phylogenomic databases

    eggNOGi NOG300333.
    GeneTreei ENSGT00530000062902.
    HOGENOMi HOG000261614.
    HOVERGENi HBG050949.
    InParanoidi Q9QUP4.
    KOi K09671.
    OMAi FPRGAIS.
    OrthoDBi EOG7RZ5S0.
    PhylomeDBi Q9QUP4.
    TreeFami TF342871.

    Enzyme and pathway databases

    Reactomei REACT_198578. Keratan sulfate biosynthesis.

    Miscellaneous databases

    NextBioi 313302.
    PROi Q9QUP4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9QUP4.
    Genevestigatori Q9QUP4.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view ]
    Pfami PF00685. Sulfotransfer_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMi SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue."
      Lee J.K., Bhakta S., Rosen S.D., Hemmerich S.
      Biochem. Biophys. Res. Commun. 263:543-549(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: C57BL/6.
      Tissue: Intestine.
    2. "Human corneal GlcNAc 6-O-sulfotransferase and mouse intestinal GlcNAc 6-O-sulfotransferase both produce keratan sulfate."
      Akama T.O., Nakayama J., Nishida K., Hiraoka N., Suzuki M., McAuliffe J., Hindsgaul O., Fukuda M., Fukuda M.N.
      J. Biol. Chem. 276:16271-16278(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, SUBSTRATE SPECIFICITY.
    3. "Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate."
      Akama T.O., Misra A.K., Hindsgaul O., Fukuda M.N.
      J. Biol. Chem. 277:42505-42513(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBSTRATE SPECIFICITY.

    Entry informationi

    Entry nameiCHST5_MOUSE
    AccessioniPrimary (citable) accession number: Q9QUP4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In human, there are 2 related proteins, CHST5 and CHST6, the latter mediating sulfation of keratan in cornea. In mouse however, there is no CHST6 protein, CHST5 functioning as a corneal keratan sulfotransferase and mediates such function.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3