Q9QUM0 (ITA2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Integrin alpha-IIb

Alternative name(s):
GPalpha IIb
Short name=GPIIb
Platelet membrane glycoprotein IIb
CD_antigen=CD41

Cleaved into the following 2 chains:

1. Integrin alpha-IIb heavy chain
2. Integrin alpha-IIb light chain

Gene names

Name:

Itga2b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1033 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
Subunit structure	Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-IIb associates with beta-3. Directly interacts with RNF181 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding
Molecular function	Integrin Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell-matrix adhesion Inferred from mutant phenotype PubMed 15031111. Source: MGI integrin-mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	external side of plasma membrane Inferred from direct assay PubMed 12900455PubMed 18971422PubMed 20228271. Source: MGI focal adhesion Inferred from direct assay PubMed 15031111. Source: MGI integrin complex Inferred from electronic annotation. Source: InterPro
Molecular_function	extracellular matrix binding Inferred from mutant phenotype PubMed 15031111. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	By similarity
	Chain	32 – 1033	1002	Integrin alpha-IIb		PRO_0000016278
	Chain	32 – ?		Integrin alpha-IIb heavy chain By similarity		PRO_0000016279
	Chain	? – 1033		Integrin alpha-IIb light chain By similarity		PRO_0000016280
Regions
	Topological domain	32 – 988	957	Extracellular Potential
	Transmembrane	989 – 1014	26	Helical; Potential
	Topological domain	1015 – 1033	19	Cytoplasmic Potential
	Repeat	35 – 96	62	FG-GAP 1
	Repeat	109 – 173	65	FG-GAP 2
	Repeat	184 – 237	54	FG-GAP 3
	Repeat	252 – 309	58	FG-GAP 4
	Repeat	310 – 370	61	FG-GAP 5
	Repeat	372 – 431	60	FG-GAP 6
	Repeat	433 – 495	63	FG-GAP 7
	Calcium binding	273 – 281	9	Potential
	Calcium binding	327 – 335	9	Potential
	Calcium binding	395 – 403	9	Potential
	Calcium binding	456 – 464	9	Potential
	Motif	1017 – 1021	5	GFFKR motif
Amino acid modifications
	Modified residue	5	1	Phosphoserine Ref.8
	Modified residue	10	1	Phosphoserine Ref.8
	Glycosylation	46	1	N-linked (GlcNAc...) Potential
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Glycosylation	600	1	N-linked (GlcNAc...) Potential
	Glycosylation	710	1	N-linked (GlcNAc...) Potential
	Glycosylation	957	1	N-linked (GlcNAc...) Potential
	Disulfide bond	87 ↔ 96		By similarity
	Disulfide bond	138 ↔ 161		By similarity
	Disulfide bond	177 ↔ 197		By similarity
	Disulfide bond	503 ↔ 514		By similarity
	Disulfide bond	520 ↔ 575		By similarity
	Disulfide bond	632 ↔ 638		By similarity
	Disulfide bond	704 ↔ 717		By similarity
	Disulfide bond	856 ↔ 905		Interchain (between heavy and light chains) By similarity
	Disulfide bond	911 ↔ 916		By similarity
Experimental info
	Sequence conflict	236	1	S → T in AAF06996. Ref.1
	Sequence conflict	236	1	S → T in AAD56216. Ref.1
	Sequence conflict	270	1	A → S in AAF06996. Ref.1
	Sequence conflict	270	1	A → S in AAD56216. Ref.1
	Sequence conflict	464	1	D → G in AAF43997. Ref.7
	Sequence conflict	471	1	G → W in AAF06996. Ref.1
	Sequence conflict	471	1	G → W in AAD56216. Ref.1
	Sequence conflict	483	1	V → G in AAF06996. Ref.1
	Sequence conflict	483	1	V → G in AAD56216. Ref.1
	Sequence conflict	805	1	R → S in AAF06996. Ref.1
	Sequence conflict	805	1	R → S in AAD56216. Ref.1
	Sequence conflict	834	1	P → H in AAD02339. Ref.6
	Sequence conflict	848 – 849	2	VQ → LR in AAD02339. Ref.6
	Sequence conflict	865	1	D → E in AAB23054. Ref.5
	Sequence conflict	933	1	V → A in AAF06996. Ref.1
	Sequence conflict	933	1	V → A in AAD56216. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9QUM0 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 7B4826E32B9130B1	FASTA	1,033	112,678
	10 20 30 40 50 60 MARASCAWHS LWLLQWTPLF LGPSAVPPVW ALNLDSEKFS VYAGPNGSHF GFSVDFHKDK 70 80 90 100 110 120 HGSVSIVVGA PRALNASQEE TGAVFLCPWK ANGGKCNPLL FDLRDETRNL GFQIFQTFKT 130 140 150 160 170 180 GQGLGASVVS WNDVIVACAP WQHWNVLEKR DEAEKTPVGG CFLAQLQSGG RAEYSPCRAN 190 200 210 220 230 240 TMSSVYAESF RGDKRYCEAG FSLAVTQAGE LVLGAPGGYF FLGLLARVPI ENIISSYRPG 250 260 270 280 290 300 TLLWHVSNQR FTYDNSNPVF FDGYRGYSVA VGEFDGDPST TEYVSGAPTW SWTLGAVEIL 310 320 330 340 350 360 DSYYQPLHRL HGEQMASYFG HSVAVTDVNG DGRHDLLVGA PLYMESRADR KLAEVGRVYL 370 380 390 400 410 420 FLQPKGPQAL STPTLLLTGT QLYGRFGSAI APLGDLNRDG YNDIAVAAPY GGPSGQGQVL 430 440 450 460 470 480 IFLGQSEGLS PRPSQVLDSP FPTGSGFGFS LRGAVDIDDN GYPDLIVGAY GASKVAVYRA 490 500 510 520 530 540 QPVVMATVQL MVQDSLNPTL KNCVLDQTKT PVSCFNIQMC VGATGHNIPQ KLHLKAELQL 550 560 570 580 590 600 DLQKPRQGRR VLLLASQQAS LTLSLDLGGR DKPICHTTGA FLRDEADFRD KLSPIVLSLN 610 620 630 640 650 660 VSLPPEETGG APAVVLHGET HVQEQTRIIL DCGEDDLCVP QLRLTATAGD SPLLIGADNV 670 680 690 700 710 720 LELKIEAAND GEGAYEAELA VHLPPGAHYM RALSNIEGFE RLVCTQKKEN ESRVALCELG 730 740 750 760 770 780 NPMKKDTRIG ITMLVSVENL EEAGESVSFQ LQVRSKNSQN PNSKVVMLPV AIQAEATVEL 790 800 810 820 830 840 RGNSFPASLV VAAEEGDREQ EDLDRWVSRL EHTYELHNIG PGTVNGLRLL IHIPGQSQPS 850 860 870 880 890 900 DLLYILDVQP QGGLLCSTQP SPKVDWKLST PSPSSIRPVH HQRERRQAFL QGPKPGQQDP 910 920 930 940 950 960 VLVSCDGSAS CTVVECELRE MVRGQRAMVT VQVMLGLSSL RQRPQEQFVL QSHAWFNVSS 970 980 990 1000 1010 1020 LPYSVPVVSL PSGQARVQTQ LLRALEERAI PVWWVLVGVL GGLLLLTLLV LAMWKAGFFK 1030 RNRPPLEEDE EEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the murine platelet alphaIIb gene and encoded cDNA." Thornton M.A., Poncz M. Blood 94:3947-3950(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Heart.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Identification of the alpha IIb beta 3 integrin in murine tumor cells." Chen Y.Q., Gao X., Timar J., Tang D., Grossi I.M., Chelladurai M., Kunicki T.J., Fligiel S.E., Taylor J.D., Honn K.V. J. Biol. Chem. 267:17314-17320(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 805-865.
[6]	Rout U.K., Armant D.R. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 790-1022.
[7]	"Multiple discontinuous ligand-mimetic antibody binding sites define a ligand binding pocket in integrin alphaIIbbeta3." Puzon-McLaughlin W., Kamata T., Takada Y. J. Biol. Chem. 275:7795-7802(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-484.
[8]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5 AND SER-10, MASS SPECTROMETRY. Tissue: Embryonic brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF169829 Genomic DNA. Translation: AAF06996.1. AF170316 mRNA. Translation: AAD56216.1. AK142289 mRNA. Translation: BAE25013.1. AK154619 mRNA. Translation: BAE32718.1. AL596258 Genomic DNA. Translation: CAM18010.1. BC120493 mRNA. Translation: AAI20494.1. S43388 mRNA. Translation: AAB23054.2. AF045019 mRNA. Translation: AAD02339.1. AF166384 mRNA. Translation: AAF43997.1.
IPI	IPI00315155.
PIR	A43430.
RefSeq	NP_034705.2. NM_010575.2.
UniGene	Mm.26646.
3D structure databases
ProteinModelPortal	Q9QUM0.
SMR	Q9QUM0. Positions 32-1024.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4098793.
PTM databases
PhosphoSite	Q9QUM0.
Proteomic databases
PaxDb	Q9QUM0.
PRIDE	Q9QUM0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000103086; ENSMUSP00000099375; ENSMUSG00000034664.
GeneID	16399.
KEGG	mmu:16399.
UCSC	uc007lrx.2. mouse.
Organism-specific databases
CTD	3674.
MGI	MGI:96601. Itga2b.
Phylogenomic databases
eggNOG	NOG26407.
GeneTree	ENSGT00700000104007.
HOGENOM	HOG000231603.
HOVERGEN	HBG006186.
InParanoid	Q3U3R7.
KO	K06476.
OMA	WSWTLGA.
OrthoDB	EOG483D3S.
Gene expression databases
Bgee	Q9QUM0.
CleanEx	MM_ITGA2B.
Genevestigator	Q9QUM0.
GermOnline	ENSMUSG00000034664. Mus musculus.
Family and domain databases
InterPro	IPR013517. FG-GAP. IPR013519. Int_alpha_beta-p. IPR000413. Integrin_alpha. IPR013649. Integrin_alpha-2. IPR018184. Integrin_alpha_C_CS. [Graphical view]
Pfam	PF01839. FG-GAP. 1 hit. PF00357. Integrin_alpha. 1 hit. PF08441. Integrin_alpha2. 1 hit. [Graphical view]
PRINTS	PR01185. INTEGRINA.
SMART	SM00191. Int_alpha. 5 hits. [Graphical view]
PROSITE	PS51470. FG_GAP. 7 hits. PS00242. INTEGRIN_ALPHA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL5253.
NextBio	289549.
SOURCE	Search...

Entry information

Entry name

ITA2B_MOUSE

Accession

Primary (citable) accession number: Q9QUM0
Secondary accession number(s): Q3U3R7, Q64229, Q9Z2M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 115 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program