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Q9QUL6

- NSF_RAT

UniProt

Q9QUL6 - NSF_RAT

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Protein
Vesicle-fusing ATPase
Gene
Nsf, Erg1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi550 – 5501Magnesium By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi505 – 5106ATP By similarity
Nucleotide bindingi545 – 5528ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent protein binding Source: RGD
  3. ATPase activity Source: UniProtKB
  4. D1 dopamine receptor binding Source: RGD
  5. Rab GTPase binding Source: RGD
  6. metal ion binding Source: UniProtKB-KW
  7. protein C-terminus binding Source: UniProtKB
  8. protein binding Source: IntAct

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. membrane fusion Source: UniProtKB
  3. neurotransmitter secretion Source: UniProtKB
  4. protein transport Source: RGD
  5. regulation of exocytosis Source: RGD
  6. vesicle fusion Source: UniProtKB
  7. vesicle-mediated transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Short name:
NEM-sensitive fusion protein
Vesicular-fusion protein NSF
Gene namesi
Name:Nsf
Synonyms:Erg1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621594. Nsf.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. Golgi stack Source: RGD
  2. dendritic shaft Source: UniProtKB
  3. postsynaptic density Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 744744Vesicle-fusing ATPase
PRO_0000263087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei105 – 1051N6-acetyllysine By similarity
Modified residuei259 – 2591Phosphotyrosine By similarity
Modified residuei569 – 5691Phosphoserine; by CDK16 By similarity

Post-translational modificationi

Phosphorylation at Ser-569 interferes with homohexamerization By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9QUL6.
PRIDEiQ9QUL6.

2D gel databases

World-2DPAGE0004:Q9QUL6.

PTM databases

PhosphoSiteiQ9QUL6.

Expressioni

Tissue specificityi

Detected in brain (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ9QUL6.

Interactioni

Subunit structurei

Homohexamer. Interacts with GABARAP and GABARAPL2 By similarity. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity By similarity. Interacts with CDK16.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gria2P194915EBI-925794,EBI-77718

Protein-protein interaction databases

BioGridi248797. 1 interaction.
IntActiQ9QUL6. 4 interactions.
MINTiMINT-4567036.

Structurei

3D structure databases

ProteinModelPortaliQ9QUL6.
SMRiQ9QUL6. Positions 1-201, 489-735.

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
HOGENOMiHOG000198544.
HOVERGENiHBG000324.
InParanoidiQ9QUL6.
KOiK06027.
PhylomeDBiQ9QUL6.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QUL6-1 [UniParc]FASTAAdd to Basket

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MAGRTMQAAR CPTDELSLSN CAVVNEKDYQ SGQHVMVRTS PNHKYIFTLR    50
THPSVVPGCI AFSLPQRKWA GLSIGQDIEV ALYSFDKAKQ CIGTMTIEID 100
FLQKKNIDSN PYDTDKMAAE FIQQFNHQAF SVGQQLVFSF NDKLFGLLVK 150
DIEAMDPSIL KGEPASGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK 200
TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC 250
KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA 300
NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV 350
VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE 400
KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS 450
TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA 500
SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT 550
ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV 600
VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD 650
VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG 700
KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALMREEGASP LDFD 744
Length:744
Mass (Da):82,652
Last modified:May 1, 2000 - v1
Checksum:i82978187FC3E0E03
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF142097 mRNA. Translation: AAD39485.1.
AF189019 mRNA. Translation: AAF01051.1.
RefSeqiNP_068516.1. NM_021748.1.
UniGeneiRn.13345.

Genome annotation databases

GeneIDi60355.
KEGGirno:60355.
UCSCiRGD:621594. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF142097 mRNA. Translation: AAD39485.1 .
AF189019 mRNA. Translation: AAF01051.1 .
RefSeqi NP_068516.1. NM_021748.1.
UniGenei Rn.13345.

3D structure databases

ProteinModelPortali Q9QUL6.
SMRi Q9QUL6. Positions 1-201, 489-735.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248797. 1 interaction.
IntActi Q9QUL6. 4 interactions.
MINTi MINT-4567036.

PTM databases

PhosphoSitei Q9QUL6.

2D gel databases

World-2DPAGE 0004:Q9QUL6.

Proteomic databases

PaxDbi Q9QUL6.
PRIDEi Q9QUL6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 60355.
KEGGi rno:60355.
UCSCi RGD:621594. rat.

Organism-specific databases

CTDi 4905.
RGDi 621594. Nsf.

Phylogenomic databases

eggNOGi COG0464.
HOGENOMi HOG000198544.
HOVERGENi HBG000324.
InParanoidi Q9QUL6.
KOi K06027.
PhylomeDBi Q9QUL6.

Miscellaneous databases

NextBioi 612027.
PROi Q9QUL6.

Gene expression databases

Genevestigatori Q9QUL6.

Family and domain databases

Gene3Di 3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Guan Z., Lu L.R., Zheng Z.C., Liu X.Y.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. Viswanathan V., Vincent S.R.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. Lubec G., Afjehi-Sadat L., Diao W.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 11-27; 69-87; 170-187; 218-232; 255-266; 316-335; 338-357; 404-413; 435-446; 534-549; 556-566; 595-631 AND 708-725, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.
  4. "Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis."
    Liu Y., Cheng K., Gong K., Fu A.K., Ip N.Y.
    J. Biol. Chem. 281:9852-9858(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDK16, TISSUE SPECIFICITY.
  5. "Plk2 attachment to NSF induces homeostatic removal of GluA2 during chronic overexcitation."
    Evers D.M., Matta J.A., Hoe H.S., Zarkowsky D., Lee S.H., Isaac J.T., Pak D.T.
    Nat. Neurosci. 13:1199-1207(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRIA2 AND NSF.

Entry informationi

Entry nameiNSF_RAT
AccessioniPrimary (citable) accession number: Q9QUL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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