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Protein

Vesicle-fusing ATPase

Gene

Nsf

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi550 – 5501MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi505 – 5106ATPBy similarity
Nucleotide bindingi545 – 5528ATPBy similarity

GO - Molecular functioni

  1. ATPase activity Source: UniProtKB
  2. ATP binding Source: UniProtKB-KW
  3. ATP-dependent protein binding Source: RGD
  4. D1 dopamine receptor binding Source: RGD
  5. metal ion binding Source: UniProtKB-KW
  6. protein C-terminus binding Source: UniProtKB
  7. Rab GTPase binding Source: RGD

GO - Biological processi

  1. Golgi to plasma membrane protein transport Source: GO_Central
  2. Golgi vesicle docking Source: GO_Central
  3. intra-Golgi vesicle-mediated transport Source: GO_Central
  4. membrane fusion Source: UniProtKB
  5. metabolic process Source: GOC
  6. neurotransmitter secretion Source: UniProtKB
  7. protein transport Source: RGD
  8. regulation of exocytosis Source: RGD
  9. vesicle fusion Source: UniProtKB
  10. vesicle-mediated transport Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-fusing ATPase (EC:3.6.4.6)
Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Short name:
NEM-sensitive fusion protein
Vesicular-fusion protein NSF
Gene namesi
Name:Nsf
Synonyms:Erg1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621594. Nsf.

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. dendritic shaft Source: UniProtKB
  2. Golgi stack Source: RGD
  3. postsynaptic density Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 744744Vesicle-fusing ATPasePRO_0000263087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei105 – 1051N6-acetyllysineBy similarity
Modified residuei259 – 2591PhosphotyrosineBy similarity
Modified residuei569 – 5691Phosphoserine; by CDK16By similarity

Post-translational modificationi

Phosphorylation at Ser-569 interferes with homohexamerization.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9QUL6.
PRIDEiQ9QUL6.

2D gel databases

World-2DPAGE0004:Q9QUL6.

PTM databases

PhosphoSiteiQ9QUL6.

Expressioni

Tissue specificityi

Detected in brain (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ9QUL6.

Interactioni

Subunit structurei

Homohexamer. Interacts with GABARAP and GABARAPL2 (By similarity). Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity (By similarity). Interacts with CDK16.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gria2P194915EBI-925794,EBI-77718

Protein-protein interaction databases

BioGridi248797. 1 interaction.
IntActiQ9QUL6. 4 interactions.
MINTiMINT-4567036.

Structurei

3D structure databases

ProteinModelPortaliQ9QUL6.
SMRiQ9QUL6. Positions 1-201, 489-735.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0464.
HOGENOMiHOG000198544.
HOVERGENiHBG000324.
InParanoidiQ9QUL6.
KOiK06027.
PhylomeDBiQ9QUL6.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QUL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGRTMQAAR CPTDELSLSN CAVVNEKDYQ SGQHVMVRTS PNHKYIFTLR
60 70 80 90 100
THPSVVPGCI AFSLPQRKWA GLSIGQDIEV ALYSFDKAKQ CIGTMTIEID
110 120 130 140 150
FLQKKNIDSN PYDTDKMAAE FIQQFNHQAF SVGQQLVFSF NDKLFGLLVK
160 170 180 190 200
DIEAMDPSIL KGEPASGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK
210 220 230 240 250
TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC
260 270 280 290 300
KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA
310 320 330 340 350
NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV
360 370 380 390 400
VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE
410 420 430 440 450
KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS
460 470 480 490 500
TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA
510 520 530 540 550
SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT
560 570 580 590 600
ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV
610 620 630 640 650
VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD
660 670 680 690 700
VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG
710 720 730 740
KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALMREEGASP LDFD
Length:744
Mass (Da):82,652
Last modified:May 1, 2000 - v1
Checksum:i82978187FC3E0E03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142097 mRNA. Translation: AAD39485.1.
AF189019 mRNA. Translation: AAF01051.1.
RefSeqiNP_068516.1. NM_021748.1.
UniGeneiRn.13345.

Genome annotation databases

GeneIDi60355.
KEGGirno:60355.
UCSCiRGD:621594. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142097 mRNA. Translation: AAD39485.1.
AF189019 mRNA. Translation: AAF01051.1.
RefSeqiNP_068516.1. NM_021748.1.
UniGeneiRn.13345.

3D structure databases

ProteinModelPortaliQ9QUL6.
SMRiQ9QUL6. Positions 1-201, 489-735.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248797. 1 interaction.
IntActiQ9QUL6. 4 interactions.
MINTiMINT-4567036.

PTM databases

PhosphoSiteiQ9QUL6.

2D gel databases

World-2DPAGE0004:Q9QUL6.

Proteomic databases

PaxDbiQ9QUL6.
PRIDEiQ9QUL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi60355.
KEGGirno:60355.
UCSCiRGD:621594. rat.

Organism-specific databases

CTDi4905.
RGDi621594. Nsf.

Phylogenomic databases

eggNOGiCOG0464.
HOGENOMiHOG000198544.
HOVERGENiHBG000324.
InParanoidiQ9QUL6.
KOiK06027.
PhylomeDBiQ9QUL6.

Miscellaneous databases

NextBioi612027.
PROiQ9QUL6.

Gene expression databases

GenevestigatoriQ9QUL6.

Family and domain databases

Gene3Di3.10.330.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR009010. Asp_de-COase-like_dom.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR004201. Cdc48_dom2.
IPR029067. CDC48_domain_2-like.
IPR003338. CDC4_N-term_subdom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 2 hits.
PF02933. CDC48_2. 1 hit.
PF02359. CDC48_N. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM01072. CDC48_2. 1 hit.
SM01073. CDC48_N. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF52540. SSF52540. 2 hits.
SSF54585. SSF54585. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Guan Z., Lu L.R., Zheng Z.C., Liu X.Y.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. Viswanathan V., Vincent S.R.
    Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. Lubec G., Afjehi-Sadat L., Diao W.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 11-27; 69-87; 170-187; 218-232; 255-266; 316-335; 338-357; 404-413; 435-446; 534-549; 556-566; 595-631 AND 708-725, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.
  4. "Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis."
    Liu Y., Cheng K., Gong K., Fu A.K., Ip N.Y.
    J. Biol. Chem. 281:9852-9858(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDK16, TISSUE SPECIFICITY.
  5. "Plk2 attachment to NSF induces homeostatic removal of GluA2 during chronic overexcitation."
    Evers D.M., Matta J.A., Hoe H.S., Zarkowsky D., Lee S.H., Isaac J.T., Pak D.T.
    Nat. Neurosci. 13:1199-1207(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRIA2 AND NSF.

Entry informationi

Entry nameiNSF_RAT
AccessioniPrimary (citable) accession number: Q9QUL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: May 1, 2000
Last modified: March 4, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.