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Q9QUL6

- NSF_RAT

UniProt

Q9QUL6 - NSF_RAT

Protein

Vesicle-fusing ATPase

Gene

Nsf

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi550 – 5501MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi505 – 5106ATPBy similarity
    Nucleotide bindingi545 – 5528ATPBy similarity

    GO - Molecular functioni

    1. ATPase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. ATP-dependent protein binding Source: RGD
    4. D1 dopamine receptor binding Source: RGD
    5. metal ion binding Source: UniProtKB-KW
    6. protein binding Source: IntAct
    7. protein C-terminus binding Source: UniProtKB
    8. Rab GTPase binding Source: RGD

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. membrane fusion Source: UniProtKB
    3. neurotransmitter secretion Source: UniProtKB
    4. protein transport Source: RGD
    5. regulation of exocytosis Source: RGD
    6. vesicle fusion Source: UniProtKB
    7. vesicle-mediated transport Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vesicle-fusing ATPase (EC:3.6.4.6)
    Alternative name(s):
    N-ethylmaleimide-sensitive fusion protein
    Short name:
    NEM-sensitive fusion protein
    Vesicular-fusion protein NSF
    Gene namesi
    Name:Nsf
    Synonyms:Erg1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621594. Nsf.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. dendritic shaft Source: UniProtKB
    2. Golgi stack Source: RGD
    3. postsynaptic density Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 744744Vesicle-fusing ATPasePRO_0000263087Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei105 – 1051N6-acetyllysineBy similarity
    Modified residuei259 – 2591PhosphotyrosineBy similarity
    Modified residuei569 – 5691Phosphoserine; by CDK16By similarity

    Post-translational modificationi

    Phosphorylation at Ser-569 interferes with homohexamerization.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9QUL6.
    PRIDEiQ9QUL6.

    2D gel databases

    World-2DPAGE0004:Q9QUL6.

    PTM databases

    PhosphoSiteiQ9QUL6.

    Expressioni

    Tissue specificityi

    Detected in brain (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriQ9QUL6.

    Interactioni

    Subunit structurei

    Homohexamer. Interacts with GABARAP and GABARAPL2 By similarity. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity By similarity. Interacts with CDK16.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Gria2P194915EBI-925794,EBI-77718

    Protein-protein interaction databases

    BioGridi248797. 1 interaction.
    IntActiQ9QUL6. 4 interactions.
    MINTiMINT-4567036.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QUL6.
    SMRiQ9QUL6. Positions 1-201, 489-735.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0464.
    HOGENOMiHOG000198544.
    HOVERGENiHBG000324.
    InParanoidiQ9QUL6.
    KOiK06027.
    PhylomeDBiQ9QUL6.

    Family and domain databases

    Gene3Di3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9QUL6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGRTMQAAR CPTDELSLSN CAVVNEKDYQ SGQHVMVRTS PNHKYIFTLR    50
    THPSVVPGCI AFSLPQRKWA GLSIGQDIEV ALYSFDKAKQ CIGTMTIEID 100
    FLQKKNIDSN PYDTDKMAAE FIQQFNHQAF SVGQQLVFSF NDKLFGLLVK 150
    DIEAMDPSIL KGEPASGKRQ KIEVGLVVGN SQVAFEKAEN SSLNLIGKAK 200
    TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF PPEIVEQMGC 250
    KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA 300
    NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV 350
    VNQLLSKIDG VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE 400
    KGRLQILHIH TARMRGHQLL SADVDIKELA VETKNFSGAE LEGLVRAAQS 450
    TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA 500
    SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL LEGPPHSGKT 550
    ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV 600
    VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD 650
    VLQEMEMLNA FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG 700
    KKVWIGIKKL LMLIEMSLQM DPEYRVRKFL ALMREEGASP LDFD 744
    Length:744
    Mass (Da):82,652
    Last modified:May 1, 2000 - v1
    Checksum:i82978187FC3E0E03
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF142097 mRNA. Translation: AAD39485.1.
    AF189019 mRNA. Translation: AAF01051.1.
    RefSeqiNP_068516.1. NM_021748.1.
    UniGeneiRn.13345.

    Genome annotation databases

    GeneIDi60355.
    KEGGirno:60355.
    UCSCiRGD:621594. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF142097 mRNA. Translation: AAD39485.1 .
    AF189019 mRNA. Translation: AAF01051.1 .
    RefSeqi NP_068516.1. NM_021748.1.
    UniGenei Rn.13345.

    3D structure databases

    ProteinModelPortali Q9QUL6.
    SMRi Q9QUL6. Positions 1-201, 489-735.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 248797. 1 interaction.
    IntActi Q9QUL6. 4 interactions.
    MINTi MINT-4567036.

    PTM databases

    PhosphoSitei Q9QUL6.

    2D gel databases

    World-2DPAGE 0004:Q9QUL6.

    Proteomic databases

    PaxDbi Q9QUL6.
    PRIDEi Q9QUL6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 60355.
    KEGGi rno:60355.
    UCSCi RGD:621594. rat.

    Organism-specific databases

    CTDi 4905.
    RGDi 621594. Nsf.

    Phylogenomic databases

    eggNOGi COG0464.
    HOGENOMi HOG000198544.
    HOVERGENi HBG000324.
    InParanoidi Q9QUL6.
    KOi K06027.
    PhylomeDBi Q9QUL6.

    Miscellaneous databases

    NextBioi 612027.
    PROi Q9QUL6.

    Gene expression databases

    Genevestigatori Q9QUL6.

    Family and domain databases

    Gene3Di 3.10.330.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR009010. Asp_de-COase-like_dom.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR004201. Cdc48_dom2.
    IPR029067. CDC48_domain_2-like.
    IPR003338. CDC4_N-term_subdom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00004. AAA. 2 hits.
    PF02933. CDC48_2. 1 hit.
    PF02359. CDC48_N. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    SM01072. CDC48_2. 1 hit.
    SM01073. CDC48_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF54585. SSF54585. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Guan Z., Lu L.R., Zheng Z.C., Liu X.Y.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.
    2. Viswanathan V., Vincent S.R.
      Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
    3. Lubec G., Afjehi-Sadat L., Diao W.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 11-27; 69-87; 170-187; 218-232; 255-266; 316-335; 338-357; 404-413; 435-446; 534-549; 556-566; 595-631 AND 708-725, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.
    4. "Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis."
      Liu Y., Cheng K., Gong K., Fu A.K., Ip N.Y.
      J. Biol. Chem. 281:9852-9858(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDK16, TISSUE SPECIFICITY.
    5. "Plk2 attachment to NSF induces homeostatic removal of GluA2 during chronic overexcitation."
      Evers D.M., Matta J.A., Hoe H.S., Zarkowsky D., Lee S.H., Isaac J.T., Pak D.T.
      Nat. Neurosci. 13:1199-1207(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GRIA2 AND NSF.

    Entry informationi

    Entry nameiNSF_RAT
    AccessioniPrimary (citable) accession number: Q9QUL6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3