Reviewed, UniProtKB/Swiss-Prot Q9QUL6 (NSF_RAT)

Names and origin

Protein names

Recommended name:
    Vesicle-fusing ATPase
    EC=3.6.4.6
Alternative name(s):
    N-ethylmaleimide-sensitive fusion protein
      Short name=NEM-sensitive fusion protein
    Vesicular-fusion protein NSF

Gene names

Name:	Nsf
Synonyms:	Erg1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homohexamer. Interacts with GABARAP and GABARAPL2 By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AAA ATPase family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	neurotransmitter secretion Traceable author statement. Source: UniProtKB protein transport Inferred from mutant phenotype. Source: RGD proteolysis Inferred from electronic annotation. Source: InterPro regulation of exocytosis Inferred from mutant phenotype. Source: RGD vesicle fusion Traceable author statement. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell dendritic shaft Inferred from direct assay. Source: UniProtKB postsynaptic density Traceable author statement. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent peptidase activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein C-terminus binding Inferred from physical interaction. Source: UniProtKB serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Gabarap	P60517	1	EBI-925794,EBI-925812
Gria2	P19491	4	EBI-925794,EBI-77718

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 744	744	Vesicle-fusing ATPase		PRO_0000263087
Regions
	Nucleotide binding	260 – 267	8	ATP Potential
	Nucleotide binding	543 – 550	8	ATP Potential
Sites
	Metal binding	550	1	Magnesium By similarity
Amino acid modifications
	Modified residue	259	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QUL6-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 82978187FC3E0E03	FASTA	744	82,652
	10 20 30 40 50 60 MAGRTMQAAR CPTDELSLSN CAVVNEKDYQ SGQHVMVRTS PNHKYIFTLR THPSVVPGCI 70 80 90 100 110 120 AFSLPQRKWA GLSIGQDIEV ALYSFDKAKQ CIGTMTIEID FLQKKNIDSN PYDTDKMAAE 130 140 150 160 170 180 FIQQFNHQAF SVGQQLVFSF NDKLFGLLVK DIEAMDPSIL KGEPASGKRQ KIEVGLVVGN 190 200 210 220 230 240 SQVAFEKAEN SSLNLIGKAK TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF 250 260 270 280 290 300 PPEIVEQMGC KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA 310 320 330 340 350 360 NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV VNQLLSKIDG 370 380 390 400 410 420 VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE KGRLQILHIH TARMRGHQLL 430 440 450 460 470 480 SADVDIKELA VETKNFSGAE LEGLVRAAQS TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF 490 500 510 520 530 540 LASLENDIKP AFGTNQEDYA SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL 550 560 570 580 590 600 LEGPPHSGKT ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV 610 620 630 640 650 660 VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD VLQEMEMLNA 670 680 690 700 710 720 FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG KKVWIGIKKL LMLIEMSLQM 730 740 DPEYRVRKFL ALMREEGASP LDFD

References

[1]	Guan Z., Lu L.R., Zheng Z.C., Liu X.Y. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	Viswanathan V., Vincent S.R. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[3]	Lubec G., Afjehi-Sadat L., Diao W. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 11-27; 69-87; 170-187; 218-232; 255-266; 316-335; 338-357; 404-413; 435-446; 534-549; 556-566; 595-631 AND 708-725, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF142097 mRNA. Translation: AAD39485.1. AF189019 mRNA. Translation: AAF01051.1.
IPI	IPI00210635.
RefSeq	NP_068516.1.
UniGene	Rn.13345
3D structure databases
HSSP	HSSP built from PDB template 1NSF based on UniProtKB P18708.
SMR	Q9QUL6. Positions 1-201, 489-735.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9QUL6. 3 interactions.
PTM databases
PhosphoSite	Q9QUL6.
Proteomic databases
PRIDE	Q9QUL6.
Genome annotation databases
Ensembl	ENSRNOG00000003905. Rattus norvegicus. [Contig view]
GeneID	60355.
KEGG	rno:60355.
Organism-specific databases
RGD	621594. Nsf.
Phylogenomic databases
HOVERGEN	Q9QUL6.
Enzyme and pathway databases
BRENDA	3.6.4.6. 248.
Gene expression databases
ArrayExpress	Q9QUL6.
GermOnline	ENSRNOG00000003905. Rattus norvegicus.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR003338. ATPase_AAA_VAT_N. IPR004201. Cell_division_protein_CDC48_2. IPR001984. Peptidase_S16_C. [Graphical view]
Pfam	PF00004. AAA. 2 hits. PF02933. CDC48_2. 1 hit. PF02359. CDC48_N. 1 hit. [Graphical view]
PRINTS	PR00830. ENDOLAPTASE.
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	612027.

Entry information

Entry name

NSF_RAT

Accession

Primary (citable) accession number: Q9QUL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)