Vesicle-fusing ATPase - Rattus norvegicus (Rat)

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Q9QUL6 (NSF_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 91. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vesicle-fusing ATPase
EC=3.6.4.6

Alternative name(s):
N-ethylmaleimide-sensitive fusion protein
Short name=NEM-sensitive fusion protein
Vesicular-fusion protein NSF

Gene names

Name:	Nsf
Synonyms:	Erg1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	744 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homohexamer. Interacts with GABARAP and GABARAPL2 By similarity. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity By similarity. Interacts with CDK16. Ref.4 Ref.5
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Detected in brain (at protein level). Ref.4
Post-translational modification	Phosphorylation at Ser-569 interferes with homohexamerization By similarity.
Sequence similarities	Belongs to the AAA ATPase family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	neurotransmitter secretion Traceable author statement PubMed 9697854. Source: UniProtKB protein transport Inferred from mutant phenotype PubMed 11245593. Source: RGD regulation of exocytosis Inferred from mutant phenotype PubMed 11245593. Source: RGD vesicle fusion Traceable author statement PubMed 9697854. Source: UniProtKB
Cellular_component	Golgi stack Inferred from direct assay PubMed 11062069. Source: RGD dendritic shaft Inferred from direct assay PubMed 1041498. Source: UniProtKB postsynaptic density Traceable author statement PubMed 9697854. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATPase activity Traceable author statement PubMed 9697854. Source: UniProtKB Rab GTPase binding Inferred from direct assay PubMed 11062069. Source: RGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Gria2	P19491	5	EBI-925794,EBI-77718

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 744

744

Vesicle-fusing ATPase

PRO_0000263087

Regions

Nucleotide binding

505 – 510

ATP By similarity

Nucleotide binding

545 – 552

ATP By similarity

Sites

Metal binding

550

Magnesium By similarity

Amino acid modifications

Modified residue

259

Phosphotyrosine By similarity

Modified residue

569

Phosphoserine; by CDK16 By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9QUL6 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 82978187FC3E0E03
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FASTA

744

82,652

        10         20         30         40         50         60 
MAGRTMQAAR CPTDELSLSN CAVVNEKDYQ SGQHVMVRTS PNHKYIFTLR THPSVVPGCI 

        70         80         90        100        110        120 
AFSLPQRKWA GLSIGQDIEV ALYSFDKAKQ CIGTMTIEID FLQKKNIDSN PYDTDKMAAE 

       130        140        150        160        170        180 
FIQQFNHQAF SVGQQLVFSF NDKLFGLLVK DIEAMDPSIL KGEPASGKRQ KIEVGLVVGN 

       190        200        210        220        230        240 
SQVAFEKAEN SSLNLIGKAK TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF 

       250        260        270        280        290        300 
PPEIVEQMGC KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA 

       310        320        330        340        350        360 
NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV VNQLLSKIDG 

       370        380        390        400        410        420 
VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE KGRLQILHIH TARMRGHQLL 

       430        440        450        460        470        480 
SADVDIKELA VETKNFSGAE LEGLVRAAQS TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF 

       490        500        510        520        530        540 
LASLENDIKP AFGTNQEDYA SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL 

       550        560        570        580        590        600 
LEGPPHSGKT ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV 

       610        620        630        640        650        660 
VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD VLQEMEMLNA 

       670        680        690        700        710        720 
FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG KKVWIGIKKL LMLIEMSLQM 

       730        740 
DPEYRVRKFL ALMREEGASP LDFD

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References

[1]	Guan Z., Lu L.R., Zheng Z.C., Liu X.Y. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[2]	Viswanathan V., Vincent S.R. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[3]	Lubec G., Afjehi-Sadat L., Diao W. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 11-27; 69-87; 170-187; 218-232; 255-266; 316-335; 338-357; 404-413; 435-446; 534-549; 556-566; 595-631 AND 708-725, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus and Spinal cord.
[4]	"Pctaire1 phosphorylates N-ethylmaleimide-sensitive fusion protein: implications in the regulation of its hexamerization and exocytosis." Liu Y., Cheng K., Gong K., Fu A.K., Ip N.Y. J. Biol. Chem. 281:9852-9858(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CDK16, TISSUE SPECIFICITY.
[5]	"Plk2 attachment to NSF induces homeostatic removal of GluA2 during chronic overexcitation." Evers D.M., Matta J.A., Hoe H.S., Zarkowsky D., Lee S.H., Isaac J.T., Pak D.T. Nat. Neurosci. 13:1199-1207(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GRIA2 AND NSF.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF142097 mRNA. Translation: AAD39485.1. AF189019 mRNA. Translation: AAF01051.1.
IPI	IPI00210635.
RefSeq	NP_068516.1. NM_021748.1.
UniGene	Rn.13345.
3D structure databases
HSSP	HSSP built from PDB template 1NSF based on UniProtKB P18708.
ProteinModelPortal	Q9QUL6.
SMR	Q9QUL6. Positions 1-201, 489-735.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9QUL6. 3 interactions.
PTM databases
PhosphoSite	Q9QUL6.
2D gel databases
World-2DPAGE	0004:Q9QUL6.
Proteomic databases
PaxDb	Q9QUL6.
PRIDE	Q9QUL6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	60355.
KEGG	rno:60355.
UCSC	RGD:621594. rat.
Organism-specific databases
CTD	4905.
RGD	621594. Nsf.
Phylogenomic databases
eggNOG	COG0464.
HOGENOM	HOG000198544.
HOVERGEN	HBG000324.
InParanoid	Q9QUL6.
KO	K06027.
OrthoDB	EOG4DZ1TR.
Gene expression databases
ArrayExpress	Q9QUL6.
Genevestigator	Q9QUL6.
GermOnline	ENSRNOG00000003905. Rattus norvegicus.
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR009010. Asp_de-COase-like_dom. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR004201. Cdc48_dom2. IPR003338. CDC4_N-term_subdom. [Graphical view]
Pfam	PF00004. AAA. 2 hits. PF02933. CDC48_2. 1 hit. PF02359. CDC48_N. 1 hit. [Graphical view]
SMART	SM00382. AAA. 2 hits. SM01072. CDC48_2. 1 hit. SM01073. CDC48_N. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	612027.

Entry information

Entry name

NSF_RAT

Accession

Primary (citable) accession number: Q9QUL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents