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Reviewed, UniProtKB/Swiss-Prot Q9QUJ7 (ACSL4_MOUSE)

Last modified January 19, 2010. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Long-chain-fatty-acid--CoA ligase 4
    EC=6.2.1.3
Alternative name(s):
    Long-chain acyl-CoA synthetase 4
      Short name=LACS 4
      Short name=mACS4
Gene names
Name: Acsl4
Synonyms: Acs4, Facl4
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.

Catalytic activity

ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactor

Magnesium By similarity.

Subcellular location

Mitochondrion outer membrane; Single-pass type III membrane protein By similarity. Peroxisome membrane; Single-pass type III membrane protein By similarity. Microsome membrane; Single-pass type III membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity.

Tissue specificity

Abundant in steroidogenic tissues, also found in the kidney, brain and liver.

Induction

Induced by adrenocorticotropic hormone (ACTH) and suppressed by glucocorticoid.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q9QUJ7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q9QUJ7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711Long-chain-fatty-acid--CoA ligase 4
PRO_0000193110

Regions

Transmembrane8 – 2821Signal-anchor for type III membrane protein Potential
Topological domain29 – 711683Cytoplasmic Potential

Amino acid modifications

Modified residue891N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 4141Missing in isoform Short.
VSP_000239

Experimental info

Sequence conflict2881L → V in CAB95965. Ref.2
Sequence conflict2931T → S in CAB95965. Ref.2
Sequence conflict430 – 4323DLV → ALL in CAB95965. Ref.2
Sequence conflict4411Y → S in CAB95965. Ref.2
Sequence conflict4581L → F in CAB95965. Ref.2
Sequence conflict5841F → S in CAB95965. Ref.2
Sequence conflict7041V → I in CAB95965. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: A90EAD489B36C1BB

FASTA71179,223
        10         20         30         40         50         60 
MNLKLNVLTI ILLPVHLLIT IYSALIFIPW YFLTNAKKKN AMAKRIKAKP TSDKPGSPYR 

        70         80         90        100        110        120 
SVTHFDSLAV IDIPGADTLD KLFDHAVAKF GKKDSLGTRE ILSEENEMQP NGKVFKKLIL 

       130        140        150        160        170        180 
GNYKWINYLE VNCRVNNFGS GLTALGLKPK NTIAIFCETR AEWMIAAQTC FKYNFPLVTL 

       190        200        210        220        230        240 
YATLGREAVV HGLNESEASY LITSVELLES KLKAALVDIN CVKHIIYVDN KTINRAEYPE 

       250        260        270        280        290        300 
GLEIHSMQSV EELGAKPENL SVPPSRPTPS DMAIVMYTSG STGRPKGLMM HHTNLIAGMT 

       310        320        330        340        350        360 
GQCERIPGLG PKDTYIGYLP LAHVLELTAE ISCFTYGCRI GYSSPLTLSD QSSKIKKGSK 

       370        380        390        400        410        420 
GDCTVLKPTL MAAVPEIMDR IYKNVMSKVQ EMNYVQKTLF KIGYDYKLEQ IKKGYDAPLC 

       430        440        450        460        470        480 
NLILFKKVKD LVGGNVRMML YGGAPLSPQT HRFMNVCLCC PIGQGYGLTE SCGAGTVTEV 

       490        500        510        520        530        540 
TDYTTGRVGA PLICCEIKLK DWQEGGYTVH DKPNPRGEIV IGGQNISMGY FKNEEKTAED 

       550        560        570        580        590        600 
YCVDENGQRW FCTGDIGEFH PDGCLQIIDR KKDLVKLQAG EYVFLGKVEA ALKNCPLIDN 

       610        620        630        640        650        660 
ICAFAKSDQS YVISFVVPNQ KKLTLLAQQK GVEGSWVDIC NNPAMEAEIL KEIREAANAM 

       670        680        690        700        710 
KLERFEIPIK VRLSPEPWTP ETGLVTDAFK LKRKELKNHY LKDVERMYGG K

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Isoform Short.

Checksum: 2CDE3B95CCE8B4C9
Show »

FASTA67074,485

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References

[1]"Regulation by adrenocorticotropic hormone and arachidonate of the expression of acyl-CoA synthetase 4, an arachidonate-preferring enzyme expressed in steroidogenic tissues."
Cho Y.-Y., Kang M.-J., Ogawa S., Yamashita Y., Fujino T., Yamamoto T.T.
Biochem. Biophys. Res. Commun. 274:741-745(2000) [PubMed: 10924347] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[2]"Identification and characterization of mouse orthologs of the AMMECR1 and FACL4 genes deleted in AMME syndrome: orthology of Xq22.3 and MmuXF1-F3."
Vitelli F., Meloni I., Fineschi S., Favara F., Tiziana Storlazzi C., Rocchi M., Renieri A.
Cytogenet. Cell Genet. 88:259-263(2000) [PubMed: 10828604] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
Tissue: Embryo.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB033887 mRNA. Translation: BAA85931.1.
AB033886 mRNA. Translation: BAA85930.1.
AB033885 mRNA. Translation: BAA85929.1.
AJ243502 mRNA. Translation: CAB95965.1.
IPIIPI00131701.
IPI00230141.
RefSeqNP_001028772.1.
NP_062350.3.
NP_997508.1.
UniGeneMm.391337

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ9QUJ7.

PTM databases

PhosphoSiteQ9QUJ7.

Proteomic databases

PRIDEQ9QUJ7.

Genome annotation databases

EnsemblENSMUST00000033634; ENSMUSP00000033634; ENSMUSG00000031278; Mus musculus. [Genome view]
ENSMUST00000112907; ENSMUSP00000108528; ENSMUSG00000031278; Mus musculus. [Genome view]
GeneID50790.
KEGGmmu:50790.

Organism-specific databases

CTD50790.
MGIMGI:1354713. Acsl4.

Phylogenomic databases

HOGENOMHBG721674.
HOVERGENQ9QUJ7.
InParanoidQ9QUJ7.
PhylomeDBQ9QUJ7.

Enzyme and pathway databases

BRENDA6.2.1.3. 244.

Gene expression databases

ArrayExpressQ9QUJ7.
BgeeQ9QUJ7.
CleanExMM_ACSL4.
GenevestigatorQ9QUJ7.
GermOnlineENSMUSG00000031278. Mus musculus.

Family and domain databases

InterProIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio307771.
SOURCESearch...

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Entry information

Entry nameACSL4_MOUSE
AccessionPrimary (citable) accession number: Q9QUJ7
Secondary accession number(s): Q9JHT4, Q9R0H3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: January 19, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents