Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Long-chain-fatty-acid--CoA ligase 4

Gene

Acsl4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

  • dendritic spine development Source: Ensembl
  • embryonic process involved in female pregnancy Source: MGI
  • fatty acid transport Source: Ensembl
  • lipid metabolic process Source: MGI
  • neuron differentiation Source: MGI
  • regulation of fatty acid metabolic process Source: MGI
  • response to interleukin-15 Source: Ensembl
  • response to nutrient Source: Ensembl
  • triglyceride metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.3. 3474.
ReactomeiR-MMU-75876. Synthesis of very long-chain fatty acyl-CoAs.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 4 (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase 4
Short name:
LACS 4
Short name:
mACS4
Gene namesi
Name:Acsl4
Synonyms:Acs4, Facl4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1354713. Acsl4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei8 – 28Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini29 – 711CytoplasmicSequence analysisAdd BLAST683

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931101 – 711Long-chain-fatty-acid--CoA ligase 4Add BLAST711

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei447PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QUJ7.
PRIDEiQ9QUJ7.

PTM databases

iPTMnetiQ9QUJ7.
PhosphoSitePlusiQ9QUJ7.

Expressioni

Tissue specificityi

Abundant in steroidogenic tissues, also found in the kidney, brain and liver.

Inductioni

Induced by adrenocorticotropic hormone (ACTH) and suppressed by glucocorticoid.

Gene expression databases

BgeeiENSMUSG00000031278.
CleanExiMM_ACSL4.
ExpressionAtlasiQ9QUJ7. baseline and differential.
GenevisibleiQ9QUJ7. MM.

Interactioni

Protein-protein interaction databases

BioGridi206122. 2 interactors.
STRINGi10090.ENSMUSP00000033634.

Structurei

3D structure databases

ProteinModelPortaliQ9QUJ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1256. Eukaryota.
COG1022. LUCA.
GeneTreeiENSGT00690000101725.
HOGENOMiHOG000159459.
HOVERGENiHBG106947.
InParanoidiQ9QUJ7.
KOiK01897.
OMAiRRVNNFG.
OrthoDBiEOG091G02F4.
TreeFamiTF314012.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: Q9QUJ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNLKLNVLTI ILLPVHLLIT IYSALIFIPW YFLTNAKKKN AMAKRIKAKP
60 70 80 90 100
TSDKPGSPYR SVTHFDSLAV IDIPGADTLD KLFDHAVAKF GKKDSLGTRE
110 120 130 140 150
ILSEENEMQP NGKVFKKLIL GNYKWINYLE VNCRVNNFGS GLTALGLKPK
160 170 180 190 200
NTIAIFCETR AEWMIAAQTC FKYNFPLVTL YATLGREAVV HGLNESEASY
210 220 230 240 250
LITSVELLES KLKAALVDIN CVKHIIYVDN KTINRAEYPE GLEIHSMQSV
260 270 280 290 300
EELGAKPENL SVPPSRPTPS DMAIVMYTSG STGRPKGVMM HHSNLIAGMT
310 320 330 340 350
GQCERIPGLG PKDTYIGYLP LAHVLELTAE ISCFTYGCRI GYSSPLTLSD
360 370 380 390 400
QSSKIKKGSK GDCTVLKPTL MAAVPEIMDR IYKNVMSKVQ EMNYVQKTLF
410 420 430 440 450
KIGYDYKLEQ IKKGYDAPLC NLILFKKVKA LLGGNVRMML SGGAPLSPQT
460 470 480 490 500
HRFMNVCFCC PIGQGYGLTE SCGAGTVTEV TDYTTGRVGA PLICCEIKLK
510 520 530 540 550
DWQEGGYTVH DKPNPRGEIV IGGQNISMGY FKNEEKTAED YCVDENGQRW
560 570 580 590 600
FCTGDIGEFH PDGCLQIIDR KKDLVKLQAG EYVSLGKVEA ALKNCPLIDN
610 620 630 640 650
ICAFAKSDQS YVISFVVPNQ KKLTLLAQQK GVEGSWVDIC NNPAMEAEIL
660 670 680 690 700
KEIREAANAM KLERFEIPIK VRLSPEPWTP ETGLVTDAFK LKRKELKNHY
710
LKDIERMYGG K
Length:711
Mass (Da):79,077
Last modified:July 27, 2011 - v2
Checksum:i84ACA29CADDD6A3A
GO
Isoform Short (identifier: Q9QUJ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: Missing.

Show »
Length:670
Mass (Da):74,339
Checksum:i017C3441FA031F48
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti288V → L in BAA85929 (PubMed:10924347).Curated1
Sequence conflicti288V → L in BAA85930 (PubMed:10924347).Curated1
Sequence conflicti288V → L in BAA85931 (PubMed:10924347).Curated1
Sequence conflicti293S → T in BAA85929 (PubMed:10924347).Curated1
Sequence conflicti293S → T in BAA85930 (PubMed:10924347).Curated1
Sequence conflicti293S → T in BAA85931 (PubMed:10924347).Curated1
Sequence conflicti430 – 432ALL → DLV in BAA85929 (PubMed:10924347).Curated3
Sequence conflicti430 – 432ALL → DLV in BAA85930 (PubMed:10924347).Curated3
Sequence conflicti430 – 432ALL → DLV in BAA85931 (PubMed:10924347).Curated3
Sequence conflicti441S → Y in BAA85929 (PubMed:10924347).Curated1
Sequence conflicti441S → Y in BAA85930 (PubMed:10924347).Curated1
Sequence conflicti441S → Y in BAA85931 (PubMed:10924347).Curated1
Sequence conflicti458F → L in BAA85929 (PubMed:10924347).Curated1
Sequence conflicti458F → L in BAA85930 (PubMed:10924347).Curated1
Sequence conflicti458F → L in BAA85931 (PubMed:10924347).Curated1
Sequence conflicti584S → F in BAA85929 (PubMed:10924347).Curated1
Sequence conflicti584S → F in BAA85930 (PubMed:10924347).Curated1
Sequence conflicti584S → F in BAA85931 (PubMed:10924347).Curated1
Sequence conflicti704I → V in BAA85929 (PubMed:10924347).Curated1
Sequence conflicti704I → V in BAA85930 (PubMed:10924347).Curated1
Sequence conflicti704I → V in BAA85931 (PubMed:10924347).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0002391 – 41Missing in isoform Short. 2 PublicationsAdd BLAST41

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB033887 mRNA. Translation: BAA85931.1.
AB033886 mRNA. Translation: BAA85930.1.
AB033885 mRNA. Translation: BAA85929.1.
AJ243502 mRNA. Translation: CAB95965.1.
AL731672 Genomic DNA. Translation: CAM15883.1.
BC058663 mRNA. Translation: AAH58663.1.
CCDSiCCDS30448.1. [Q9QUJ7-1]
CCDS41156.1. [Q9QUJ7-2]
RefSeqiNP_001028772.1. NM_001033600.1. [Q9QUJ7-2]
NP_062350.3. NM_019477.3. [Q9QUJ7-2]
NP_997508.1. NM_207625.2. [Q9QUJ7-1]
XP_011246144.1. XM_011247842.2. [Q9QUJ7-2]
XP_011246145.1. XM_011247843.2. [Q9QUJ7-2]
UniGeneiMm.391337.

Genome annotation databases

EnsembliENSMUST00000033634; ENSMUSP00000033634; ENSMUSG00000031278. [Q9QUJ7-1]
ENSMUST00000112903; ENSMUSP00000108524; ENSMUSG00000031278. [Q9QUJ7-2]
ENSMUST00000112904; ENSMUSP00000108525; ENSMUSG00000031278. [Q9QUJ7-2]
ENSMUST00000112907; ENSMUSP00000108528; ENSMUSG00000031278. [Q9QUJ7-1]
GeneIDi50790.
KEGGimmu:50790.
UCSCiuc009ulu.1. mouse. [Q9QUJ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB033887 mRNA. Translation: BAA85931.1.
AB033886 mRNA. Translation: BAA85930.1.
AB033885 mRNA. Translation: BAA85929.1.
AJ243502 mRNA. Translation: CAB95965.1.
AL731672 Genomic DNA. Translation: CAM15883.1.
BC058663 mRNA. Translation: AAH58663.1.
CCDSiCCDS30448.1. [Q9QUJ7-1]
CCDS41156.1. [Q9QUJ7-2]
RefSeqiNP_001028772.1. NM_001033600.1. [Q9QUJ7-2]
NP_062350.3. NM_019477.3. [Q9QUJ7-2]
NP_997508.1. NM_207625.2. [Q9QUJ7-1]
XP_011246144.1. XM_011247842.2. [Q9QUJ7-2]
XP_011246145.1. XM_011247843.2. [Q9QUJ7-2]
UniGeneiMm.391337.

3D structure databases

ProteinModelPortaliQ9QUJ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206122. 2 interactors.
STRINGi10090.ENSMUSP00000033634.

PTM databases

iPTMnetiQ9QUJ7.
PhosphoSitePlusiQ9QUJ7.

Proteomic databases

PaxDbiQ9QUJ7.
PRIDEiQ9QUJ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033634; ENSMUSP00000033634; ENSMUSG00000031278. [Q9QUJ7-1]
ENSMUST00000112903; ENSMUSP00000108524; ENSMUSG00000031278. [Q9QUJ7-2]
ENSMUST00000112904; ENSMUSP00000108525; ENSMUSG00000031278. [Q9QUJ7-2]
ENSMUST00000112907; ENSMUSP00000108528; ENSMUSG00000031278. [Q9QUJ7-1]
GeneIDi50790.
KEGGimmu:50790.
UCSCiuc009ulu.1. mouse. [Q9QUJ7-1]

Organism-specific databases

CTDi2182.
MGIiMGI:1354713. Acsl4.

Phylogenomic databases

eggNOGiKOG1256. Eukaryota.
COG1022. LUCA.
GeneTreeiENSGT00690000101725.
HOGENOMiHOG000159459.
HOVERGENiHBG106947.
InParanoidiQ9QUJ7.
KOiK01897.
OMAiRRVNNFG.
OrthoDBiEOG091G02F4.
TreeFamiTF314012.

Enzyme and pathway databases

BRENDAi6.2.1.3. 3474.
ReactomeiR-MMU-75876. Synthesis of very long-chain fatty acyl-CoAs.

Miscellaneous databases

ChiTaRSiAcsl4. mouse.
PROiQ9QUJ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031278.
CleanExiMM_ACSL4.
ExpressionAtlasiQ9QUJ7. baseline and differential.
GenevisibleiQ9QUJ7. MM.

Family and domain databases

InterProiIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACSL4_MOUSE
AccessioniPrimary (citable) accession number: Q9QUJ7
Secondary accession number(s): Q5D071, Q9JHT4, Q9R0H3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.