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Q9QUJ7 (ACSL4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Long-chain-fatty-acid--CoA ligase 4

EC=6.2.1.3
Alternative name(s):
Long-chain acyl-CoA synthetase 4
Short name=LACS 4
Short name=mACS4
Gene names
Name:Acsl4
Synonyms:Acs4, Facl4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.

Catalytic activity

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactor

Magnesium By similarity.

Subcellular location

Mitochondrion outer membrane; Single-pass type III membrane protein By similarity. Peroxisome membrane; Single-pass type III membrane protein By similarity. Microsome membrane; Single-pass type III membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass type III membrane protein By similarity.

Tissue specificity

Abundant in steroidogenic tissues, also found in the kidney, brain and liver.

Induction

Induced by adrenocorticotropic hormone (ACTH) and suppressed by glucocorticoid.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
   Biological processFatty acid metabolism
Lipid metabolism
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
Mitochondrion
Mitochondrion outer membrane
Peroxisome
   Coding sequence diversityAlternative splicing
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandATP-binding
Magnesium
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdendritic spine development

Inferred from electronic annotation. Source: Ensembl

embryonic process involved in female pregnancy

Inferred from mutant phenotype PubMed 11409893. Source: MGI

fatty acid transport

Inferred from electronic annotation. Source: Ensembl

lipid biosynthetic process

Inferred from electronic annotation. Source: Ensembl

negative regulation of prostaglandin secretion

Inferred from electronic annotation. Source: Ensembl

neuron differentiation

Inferred from mutant phenotype PubMed 22366036. Source: MGI

positive regulation of cell growth

Inferred from electronic annotation. Source: Ensembl

regulation of fatty acid metabolic process

Traceable author statement PubMed 10669417. Source: MGI

response to interleukin-15

Inferred from electronic annotation. Source: Ensembl

response to nutrient

Inferred from electronic annotation. Source: Ensembl

triglyceride metabolic process

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

lipid particle

Inferred from electronic annotation. Source: Ensembl

mitochondrial outer membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

mitochondrion

Inferred from direct assay PubMed 18614015PubMed 23159612. Source: MGI

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

peroxisomal membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

arachidonate-CoA ligase activity

Inferred from electronic annotation. Source: Ensembl

long-chain fatty acid-CoA ligase activity

Inferred from direct assay PubMed 23159612. Source: MGI

very long-chain fatty acid-CoA ligase activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q9QUJ7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q9QUJ7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711Long-chain-fatty-acid--CoA ligase 4
PRO_0000193110

Regions

Transmembrane8 – 2821Helical; Signal-anchor for type III membrane protein; Potential
Topological domain29 – 711683Cytoplasmic Potential

Natural variations

Alternative sequence1 – 4141Missing in isoform Short.
VSP_000239

Experimental info

Sequence conflict2881V → L in BAA85929. Ref.1
Sequence conflict2881V → L in BAA85930. Ref.1
Sequence conflict2881V → L in BAA85931. Ref.1
Sequence conflict2931S → T in BAA85929. Ref.1
Sequence conflict2931S → T in BAA85930. Ref.1
Sequence conflict2931S → T in BAA85931. Ref.1
Sequence conflict430 – 4323ALL → DLV in BAA85929. Ref.1
Sequence conflict430 – 4323ALL → DLV in BAA85930. Ref.1
Sequence conflict430 – 4323ALL → DLV in BAA85931. Ref.1
Sequence conflict4411S → Y in BAA85929. Ref.1
Sequence conflict4411S → Y in BAA85930. Ref.1
Sequence conflict4411S → Y in BAA85931. Ref.1
Sequence conflict4581F → L in BAA85929. Ref.1
Sequence conflict4581F → L in BAA85930. Ref.1
Sequence conflict4581F → L in BAA85931. Ref.1
Sequence conflict5841S → F in BAA85929. Ref.1
Sequence conflict5841S → F in BAA85930. Ref.1
Sequence conflict5841S → F in BAA85931. Ref.1
Sequence conflict7041I → V in BAA85929. Ref.1
Sequence conflict7041I → V in BAA85930. Ref.1
Sequence conflict7041I → V in BAA85931. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 84ACA29CADDD6A3A

FASTA71179,077
        10         20         30         40         50         60 
MNLKLNVLTI ILLPVHLLIT IYSALIFIPW YFLTNAKKKN AMAKRIKAKP TSDKPGSPYR 

        70         80         90        100        110        120 
SVTHFDSLAV IDIPGADTLD KLFDHAVAKF GKKDSLGTRE ILSEENEMQP NGKVFKKLIL 

       130        140        150        160        170        180 
GNYKWINYLE VNCRVNNFGS GLTALGLKPK NTIAIFCETR AEWMIAAQTC FKYNFPLVTL 

       190        200        210        220        230        240 
YATLGREAVV HGLNESEASY LITSVELLES KLKAALVDIN CVKHIIYVDN KTINRAEYPE 

       250        260        270        280        290        300 
GLEIHSMQSV EELGAKPENL SVPPSRPTPS DMAIVMYTSG STGRPKGVMM HHSNLIAGMT 

       310        320        330        340        350        360 
GQCERIPGLG PKDTYIGYLP LAHVLELTAE ISCFTYGCRI GYSSPLTLSD QSSKIKKGSK 

       370        380        390        400        410        420 
GDCTVLKPTL MAAVPEIMDR IYKNVMSKVQ EMNYVQKTLF KIGYDYKLEQ IKKGYDAPLC 

       430        440        450        460        470        480 
NLILFKKVKA LLGGNVRMML SGGAPLSPQT HRFMNVCFCC PIGQGYGLTE SCGAGTVTEV 

       490        500        510        520        530        540 
TDYTTGRVGA PLICCEIKLK DWQEGGYTVH DKPNPRGEIV IGGQNISMGY FKNEEKTAED 

       550        560        570        580        590        600 
YCVDENGQRW FCTGDIGEFH PDGCLQIIDR KKDLVKLQAG EYVSLGKVEA ALKNCPLIDN 

       610        620        630        640        650        660 
ICAFAKSDQS YVISFVVPNQ KKLTLLAQQK GVEGSWVDIC NNPAMEAEIL KEIREAANAM 

       670        680        690        700        710 
KLERFEIPIK VRLSPEPWTP ETGLVTDAFK LKRKELKNHY LKDIERMYGG K 

« Hide

Isoform Short [UniParc].

Checksum: 017C3441FA031F48
Show »

FASTA67074,339

References

« Hide 'large scale' references
[1]"Regulation by adrenocorticotropic hormone and arachidonate of the expression of acyl-CoA synthetase 4, an arachidonate-preferring enzyme expressed in steroidogenic tissues."
Cho Y.-Y., Kang M.-J., Ogawa S., Yamashita Y., Fujino T., Yamamoto T.T.
Biochem. Biophys. Res. Commun. 274:741-745(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
[2]"Identification and characterization of mouse orthologs of the AMMECR1 and FACL4 genes deleted in AMME syndrome: orthology of Xq22.3 and MmuXF1-F3."
Vitelli F., Meloni I., Fineschi S., Favara F., Tiziana Storlazzi C., Rocchi M., Renieri A.
Cytogenet. Cell Genet. 88:259-263(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
Tissue: Embryo.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB033887 mRNA. Translation: BAA85931.1.
AB033886 mRNA. Translation: BAA85930.1.
AB033885 mRNA. Translation: BAA85929.1.
AJ243502 mRNA. Translation: CAB95965.1.
AL731672 Genomic DNA. Translation: CAM15883.1.
BC058663 mRNA. Translation: AAH58663.1.
CCDSCCDS30448.1. [Q9QUJ7-1]
CCDS41156.1. [Q9QUJ7-2]
RefSeqNP_001028772.1. NM_001033600.1. [Q9QUJ7-2]
NP_062350.3. NM_019477.3. [Q9QUJ7-2]
NP_997508.1. NM_207625.2. [Q9QUJ7-1]
XP_006528969.1. XM_006528906.1. [Q9QUJ7-2]
XP_006528970.1. XM_006528907.1. [Q9QUJ7-2]
UniGeneMm.391337.

3D structure databases

ProteinModelPortalQ9QUJ7.
SMRQ9QUJ7. Positions 122-598.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid206122. 2 interactions.

PTM databases

PhosphoSiteQ9QUJ7.

Proteomic databases

MaxQBQ9QUJ7.
PaxDbQ9QUJ7.
PRIDEQ9QUJ7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033634; ENSMUSP00000033634; ENSMUSG00000031278. [Q9QUJ7-1]
ENSMUST00000112903; ENSMUSP00000108524; ENSMUSG00000031278. [Q9QUJ7-2]
ENSMUST00000112904; ENSMUSP00000108525; ENSMUSG00000031278. [Q9QUJ7-2]
ENSMUST00000112907; ENSMUSP00000108528; ENSMUSG00000031278. [Q9QUJ7-1]
GeneID50790.
KEGGmmu:50790.
UCSCuc009ulu.1. mouse. [Q9QUJ7-1]

Organism-specific databases

CTD2182.
MGIMGI:1354713. Acsl4.

Phylogenomic databases

eggNOGCOG1022.
GeneTreeENSGT00690000102168.
HOGENOMHOG000159459.
HOVERGENHBG106947.
InParanoidQ9QUJ7.
KOK01897.
OMANAMKLER.
OrthoDBEOG7P2XRD.
TreeFamTF314012.

Enzyme and pathway databases

BRENDA6.2.1.3. 3474.

Gene expression databases

ArrayExpressQ9QUJ7.
BgeeQ9QUJ7.
CleanExMM_ACSL4.
GenevestigatorQ9QUJ7.

Family and domain databases

InterProIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSACSL4. mouse.
NextBio307771.
PROQ9QUJ7.
SOURCESearch...

Entry information

Entry nameACSL4_MOUSE
AccessionPrimary (citable) accession number: Q9QUJ7
Secondary accession number(s): Q5D071, Q9JHT4, Q9R0H3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot