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Q9QUJ7

- ACSL4_MOUSE

UniProt

Q9QUJ7 - ACSL4_MOUSE

Protein

Long-chain-fatty-acid--CoA ligase 4

Gene

Acsl4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

    Cofactori

    Magnesium.By similarity

    GO - Molecular functioni

    1. arachidonate-CoA ligase activity Source: Ensembl
    2. ATP binding Source: UniProtKB-KW
    3. long-chain fatty acid-CoA ligase activity Source: MGI
    4. very long-chain fatty acid-CoA ligase activity Source: Ensembl

    GO - Biological processi

    1. dendritic spine development Source: Ensembl
    2. embryonic process involved in female pregnancy Source: MGI
    3. fatty acid transport Source: Ensembl
    4. lipid biosynthetic process Source: Ensembl
    5. negative regulation of prostaglandin secretion Source: Ensembl
    6. neuron differentiation Source: MGI
    7. positive regulation of cell growth Source: Ensembl
    8. regulation of fatty acid metabolic process Source: MGI
    9. response to interleukin-15 Source: Ensembl
    10. response to nutrient Source: Ensembl
    11. triglyceride metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi6.2.1.3. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long-chain-fatty-acid--CoA ligase 4 (EC:6.2.1.3)
    Alternative name(s):
    Long-chain acyl-CoA synthetase 4
    Short name:
    LACS 4
    Short name:
    mACS4
    Gene namesi
    Name:Acsl4
    Synonyms:Acs4, Facl4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1354713. Acsl4.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. lipid particle Source: Ensembl
    4. mitochondrial outer membrane Source: UniProtKB-SubCell
    5. mitochondrion Source: MGI
    6. neuronal cell body Source: Ensembl
    7. peroxisomal membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 711711Long-chain-fatty-acid--CoA ligase 4PRO_0000193110Add
    BLAST

    Proteomic databases

    MaxQBiQ9QUJ7.
    PaxDbiQ9QUJ7.
    PRIDEiQ9QUJ7.

    PTM databases

    PhosphoSiteiQ9QUJ7.

    Expressioni

    Tissue specificityi

    Abundant in steroidogenic tissues, also found in the kidney, brain and liver.

    Inductioni

    Induced by adrenocorticotropic hormone (ACTH) and suppressed by glucocorticoid.

    Gene expression databases

    ArrayExpressiQ9QUJ7.
    BgeeiQ9QUJ7.
    CleanExiMM_ACSL4.
    GenevestigatoriQ9QUJ7.

    Interactioni

    Protein-protein interaction databases

    BioGridi206122. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QUJ7.
    SMRiQ9QUJ7. Positions 122-598.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini29 – 711683CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821Helical; Signal-anchor for type III membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1022.
    GeneTreeiENSGT00690000102168.
    HOGENOMiHOG000159459.
    HOVERGENiHBG106947.
    InParanoidiQ9QUJ7.
    KOiK01897.
    OMAiNAMKLER.
    OrthoDBiEOG7P2XRD.
    TreeFamiTF314012.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q9QUJ7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNLKLNVLTI ILLPVHLLIT IYSALIFIPW YFLTNAKKKN AMAKRIKAKP    50
    TSDKPGSPYR SVTHFDSLAV IDIPGADTLD KLFDHAVAKF GKKDSLGTRE 100
    ILSEENEMQP NGKVFKKLIL GNYKWINYLE VNCRVNNFGS GLTALGLKPK 150
    NTIAIFCETR AEWMIAAQTC FKYNFPLVTL YATLGREAVV HGLNESEASY 200
    LITSVELLES KLKAALVDIN CVKHIIYVDN KTINRAEYPE GLEIHSMQSV 250
    EELGAKPENL SVPPSRPTPS DMAIVMYTSG STGRPKGVMM HHSNLIAGMT 300
    GQCERIPGLG PKDTYIGYLP LAHVLELTAE ISCFTYGCRI GYSSPLTLSD 350
    QSSKIKKGSK GDCTVLKPTL MAAVPEIMDR IYKNVMSKVQ EMNYVQKTLF 400
    KIGYDYKLEQ IKKGYDAPLC NLILFKKVKA LLGGNVRMML SGGAPLSPQT 450
    HRFMNVCFCC PIGQGYGLTE SCGAGTVTEV TDYTTGRVGA PLICCEIKLK 500
    DWQEGGYTVH DKPNPRGEIV IGGQNISMGY FKNEEKTAED YCVDENGQRW 550
    FCTGDIGEFH PDGCLQIIDR KKDLVKLQAG EYVSLGKVEA ALKNCPLIDN 600
    ICAFAKSDQS YVISFVVPNQ KKLTLLAQQK GVEGSWVDIC NNPAMEAEIL 650
    KEIREAANAM KLERFEIPIK VRLSPEPWTP ETGLVTDAFK LKRKELKNHY 700
    LKDIERMYGG K 711
    Length:711
    Mass (Da):79,077
    Last modified:July 27, 2011 - v2
    Checksum:i84ACA29CADDD6A3A
    GO
    Isoform Short (identifier: Q9QUJ7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-41: Missing.

    Show »
    Length:670
    Mass (Da):74,339
    Checksum:i017C3441FA031F48
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti288 – 2881V → L in BAA85929. (PubMed:10924347)Curated
    Sequence conflicti288 – 2881V → L in BAA85930. (PubMed:10924347)Curated
    Sequence conflicti288 – 2881V → L in BAA85931. (PubMed:10924347)Curated
    Sequence conflicti293 – 2931S → T in BAA85929. (PubMed:10924347)Curated
    Sequence conflicti293 – 2931S → T in BAA85930. (PubMed:10924347)Curated
    Sequence conflicti293 – 2931S → T in BAA85931. (PubMed:10924347)Curated
    Sequence conflicti430 – 4323ALL → DLV in BAA85929. (PubMed:10924347)Curated
    Sequence conflicti430 – 4323ALL → DLV in BAA85930. (PubMed:10924347)Curated
    Sequence conflicti430 – 4323ALL → DLV in BAA85931. (PubMed:10924347)Curated
    Sequence conflicti441 – 4411S → Y in BAA85929. (PubMed:10924347)Curated
    Sequence conflicti441 – 4411S → Y in BAA85930. (PubMed:10924347)Curated
    Sequence conflicti441 – 4411S → Y in BAA85931. (PubMed:10924347)Curated
    Sequence conflicti458 – 4581F → L in BAA85929. (PubMed:10924347)Curated
    Sequence conflicti458 – 4581F → L in BAA85930. (PubMed:10924347)Curated
    Sequence conflicti458 – 4581F → L in BAA85931. (PubMed:10924347)Curated
    Sequence conflicti584 – 5841S → F in BAA85929. (PubMed:10924347)Curated
    Sequence conflicti584 – 5841S → F in BAA85930. (PubMed:10924347)Curated
    Sequence conflicti584 – 5841S → F in BAA85931. (PubMed:10924347)Curated
    Sequence conflicti704 – 7041I → V in BAA85929. (PubMed:10924347)Curated
    Sequence conflicti704 – 7041I → V in BAA85930. (PubMed:10924347)Curated
    Sequence conflicti704 – 7041I → V in BAA85931. (PubMed:10924347)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4141Missing in isoform Short. 2 PublicationsVSP_000239Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB033887 mRNA. Translation: BAA85931.1.
    AB033886 mRNA. Translation: BAA85930.1.
    AB033885 mRNA. Translation: BAA85929.1.
    AJ243502 mRNA. Translation: CAB95965.1.
    AL731672 Genomic DNA. Translation: CAM15883.1.
    BC058663 mRNA. Translation: AAH58663.1.
    CCDSiCCDS30448.1. [Q9QUJ7-1]
    CCDS41156.1. [Q9QUJ7-2]
    RefSeqiNP_001028772.1. NM_001033600.1. [Q9QUJ7-2]
    NP_062350.3. NM_019477.3. [Q9QUJ7-2]
    NP_997508.1. NM_207625.2. [Q9QUJ7-1]
    XP_006528969.1. XM_006528906.1. [Q9QUJ7-2]
    XP_006528970.1. XM_006528907.1. [Q9QUJ7-2]
    UniGeneiMm.391337.

    Genome annotation databases

    EnsembliENSMUST00000033634; ENSMUSP00000033634; ENSMUSG00000031278. [Q9QUJ7-1]
    ENSMUST00000112903; ENSMUSP00000108524; ENSMUSG00000031278. [Q9QUJ7-2]
    ENSMUST00000112904; ENSMUSP00000108525; ENSMUSG00000031278. [Q9QUJ7-2]
    ENSMUST00000112907; ENSMUSP00000108528; ENSMUSG00000031278. [Q9QUJ7-1]
    GeneIDi50790.
    KEGGimmu:50790.
    UCSCiuc009ulu.1. mouse. [Q9QUJ7-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB033887 mRNA. Translation: BAA85931.1 .
    AB033886 mRNA. Translation: BAA85930.1 .
    AB033885 mRNA. Translation: BAA85929.1 .
    AJ243502 mRNA. Translation: CAB95965.1 .
    AL731672 Genomic DNA. Translation: CAM15883.1 .
    BC058663 mRNA. Translation: AAH58663.1 .
    CCDSi CCDS30448.1. [Q9QUJ7-1 ]
    CCDS41156.1. [Q9QUJ7-2 ]
    RefSeqi NP_001028772.1. NM_001033600.1. [Q9QUJ7-2 ]
    NP_062350.3. NM_019477.3. [Q9QUJ7-2 ]
    NP_997508.1. NM_207625.2. [Q9QUJ7-1 ]
    XP_006528969.1. XM_006528906.1. [Q9QUJ7-2 ]
    XP_006528970.1. XM_006528907.1. [Q9QUJ7-2 ]
    UniGenei Mm.391337.

    3D structure databases

    ProteinModelPortali Q9QUJ7.
    SMRi Q9QUJ7. Positions 122-598.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 206122. 2 interactions.

    PTM databases

    PhosphoSitei Q9QUJ7.

    Proteomic databases

    MaxQBi Q9QUJ7.
    PaxDbi Q9QUJ7.
    PRIDEi Q9QUJ7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033634 ; ENSMUSP00000033634 ; ENSMUSG00000031278 . [Q9QUJ7-1 ]
    ENSMUST00000112903 ; ENSMUSP00000108524 ; ENSMUSG00000031278 . [Q9QUJ7-2 ]
    ENSMUST00000112904 ; ENSMUSP00000108525 ; ENSMUSG00000031278 . [Q9QUJ7-2 ]
    ENSMUST00000112907 ; ENSMUSP00000108528 ; ENSMUSG00000031278 . [Q9QUJ7-1 ]
    GeneIDi 50790.
    KEGGi mmu:50790.
    UCSCi uc009ulu.1. mouse. [Q9QUJ7-1 ]

    Organism-specific databases

    CTDi 2182.
    MGIi MGI:1354713. Acsl4.

    Phylogenomic databases

    eggNOGi COG1022.
    GeneTreei ENSGT00690000102168.
    HOGENOMi HOG000159459.
    HOVERGENi HBG106947.
    InParanoidi Q9QUJ7.
    KOi K01897.
    OMAi NAMKLER.
    OrthoDBi EOG7P2XRD.
    TreeFami TF314012.

    Enzyme and pathway databases

    BRENDAi 6.2.1.3. 3474.

    Miscellaneous databases

    ChiTaRSi ACSL4. mouse.
    NextBioi 307771.
    PROi Q9QUJ7.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9QUJ7.
    Bgeei Q9QUJ7.
    CleanExi MM_ACSL4.
    Genevestigatori Q9QUJ7.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Regulation by adrenocorticotropic hormone and arachidonate of the expression of acyl-CoA synthetase 4, an arachidonate-preferring enzyme expressed in steroidogenic tissues."
      Cho Y.-Y., Kang M.-J., Ogawa S., Yamashita Y., Fujino T., Yamamoto T.T.
      Biochem. Biophys. Res. Commun. 274:741-745(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT).
    2. "Identification and characterization of mouse orthologs of the AMMECR1 and FACL4 genes deleted in AMME syndrome: orthology of Xq22.3 and MmuXF1-F3."
      Vitelli F., Meloni I., Fineschi S., Favara F., Tiziana Storlazzi C., Rocchi M., Renieri A.
      Cytogenet. Cell Genet. 88:259-263(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
      Tissue: Embryo.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiACSL4_MOUSE
    AccessioniPrimary (citable) accession number: Q9QUJ7
    Secondary accession number(s): Q5D071, Q9JHT4, Q9R0H3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3