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Q9QUI6 (ACKR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Duffy antigen/chemokine receptor
Alternative name(s):
CD_antigen=CD234
Gene names
Name:Darc
Synonyms:Ackr1, Dfy, Fy
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1 and TARC. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms: when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes; when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels By similarity.

Subcellular location

Early endosome By similarity. Recycling endosome By similarity. Membrane; Multi-pass membrane protein. Note: Predominantly localizes to endocytic vesicles, and upon stimulation by the ligand is internalized via caveolae. Once internalized, the ligand dissociates from the receptor, and is targeted to degradation while the receptor is recycled back to the cell membrane By similarity.

Tissue specificity

Expressed in liver and brain.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Atypical chemokine receptor subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Duffy antigen/chemokine receptor
PRO_0000152587

Regions

Topological domain1 – 6161Extracellular Potential
Transmembrane62 – 8221Helical; Name=1; Potential
Topological domain83 – 9311Cytoplasmic Potential
Transmembrane94 – 11421Helical; Name=2; Potential
Topological domain115 – 12713Extracellular Potential
Transmembrane128 – 15124Helical; Name=3; Potential
Topological domain152 – 16413Cytoplasmic Potential
Transmembrane165 – 18521Helical; Name=4; Potential
Topological domain186 – 20520Extracellular Potential
Transmembrane206 – 22621Helical; Name=5; Potential
Topological domain227 – 24216Cytoplasmic Potential
Transmembrane243 – 26321Helical; Name=6; Potential
Topological domain264 – 28522Extracellular Potential
Transmembrane286 – 30621Helical; Name=7; Potential
Topological domain307 – 33428Cytoplasmic Potential

Amino acid modifications

Glycosylation161N-linked (GlcNAc...) Potential
Glycosylation261N-linked (GlcNAc...) Potential
Glycosylation321N-linked (GlcNAc...) Potential
Glycosylation2851N-linked (GlcNAc...) Potential
Disulfide bond49 ↔ 274 By similarity
Disulfide bond127 ↔ 193 By similarity

Natural variations

Natural variant101T → N in strain: BLG2/Msf, C57BL/10SnJ, HMI/Msf, MSM/Msf, NJL/Msf and SWN/Msf.
Natural variant561D → G in strain: BLG2/Msf, C57BL/10SnJ, HMI/Msf, MSM/Msf, NJL/Msf and SWN/Msf.
Natural variant611P → L in strain: BLG2/Msf.
Natural variant761S → G in strain: BLG2/Msf, C57BL/10SnJ, CAST/Ei, HMI/Msf, MSM/Msf, NJL/Msf and SWN/Msf.
Natural variant1781S → L in strain: BLG2/Msf, C57BL/10SnJ, CAST/Ei, HMI/Msf, MSM/Msf, NJL/Msf, pgn2 and SWN/Msf.
Natural variant1881V → A in strain: BLG2/Msf, C57BL/10SnJ, CAST/Ei, HMI/Msf, MSM/Msf, NJL/Msf and SWN/Msf.
Natural variant1941T → A in strain: BLG2/Msf, C57BL/10SnJ, HMI/Msf, MSM/Msf, NJL/Msf and SWN/Msf.
Natural variant2071T → M in strain: C57BL/10SnJ, HMI/Msf, MSM/Msf and SWN/Msf.
Natural variant2211L → P in strain: BLG2/Msf, C57BL/10SnJ, HMI/Msf, MSM/Msf, NJL/Msf and SWN/Msf.
Natural variant2561M → I in strain: NJL/Msf.
Natural variant2681T → I in strain: BLG2/Msf, C57BL/10SnJ, HMI/Msf, MSM/Msf, NJL/Msf and SWN/Msf.

Sequences

Sequence LengthMass (Da)Tools
Q9QUI6 [UniParc].

Last modified January 10, 2003. Version 2.
Checksum: 21AC4B6147D840C8

FASTA33436,694
        10         20         30         40         50         60 
MGNCLYPVET LSLDKNGTQF TFDSWNYSFE DNYSYELSSD YSLTPAAPCY SCNLLDRSSL 

        70         80         90        100        110        120 
PFFMLTSVLG MLASGSILFA ILRPFFHWQI CPSWPILAEL AVGSALFSIA VPILAPGLHS 

       130        140        150        160        170        180 
AHSTALCNLG YWVWYTSAFA QALLIGCYAC LNPRLNIGQL RGFTLGLSVG LWGAAALSGL 

       190        200        210        220        230        240 
PVALASDVYN GFCTFPSSRD MEALKYTHYA ICFTIFTVLP LTLLAAKGLK IALSKGPGPW 

       250        260        270        280        290        300 
VSVLWIWFIF WWPHGMVLIF DALVRSKTVL LYTCQSQKIL DAMLNVTEAL SMLHCVATPL 

       310        320        330 
LLALFCHQTT RRSLSSLSLP TRQASQMDAL AGKS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, characterization, and mapping of a murine promiscuous chemokine receptor gene: homolog of the human Duffy gene."
Luo H., Chaudhuri A., Johnson K.R., Neote K., Zbrzezna V., He Y., Pogo A.O.
Genome Res. 7:932-941(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]"Murine skeletal muscle duffy antigen/receptor for chemokine glycoprotein (DARC)."
Tournamille C., Colin Y.
Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Skeletal muscle.
[3]"Molecular cloning and characterization of a mouse gene with homology to the Duffy-antigen receptor for chemokines."
Tang T., Owen J.D., Du J., Walker C.L., Richmond A.
DNA Seq. 9:129-143(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Conspicuous differences among gene genealogies of 21 nuclear genes of five Mus musculus subspecies."
Liu Y., Kitano T., Koide T., Shiroishi T., Moriwaki K., Saitou N.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: BFM/2Msf, BLG2/Msf, C57BL/10SnJ, CAST/Ei, HMI/Msf, MSM/Msf, NJL/Msf, Pgn2 and SWN/Msf.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF016697 Genomic DNA. Translation: AAC53360.1.
AF016584 mRNA. Translation: AAC53354.1.
AF109159 mRNA. Translation: AAF14225.1.
U88431 Genomic DNA. Translation: AAF08328.1.
AB039074 Genomic DNA. Translation: BAB68598.1.
AB039075 Genomic DNA. Translation: BAB68599.1.
AB039076 Genomic DNA. Translation: BAB68600.1.
AB039077 Genomic DNA. Translation: BAB68601.1.
AB039078 Genomic DNA. Translation: BAB68602.1.
AB039079 Genomic DNA. Translation: BAB68603.1.
AB039080 Genomic DNA. Translation: BAB68604.1.
AB039081 Genomic DNA. Translation: BAB68605.1.
AB039082 Genomic DNA. Translation: BAB68606.1.
BC005583 mRNA. Translation: AAH05583.1.
RefSeqNP_034175.2. NM_010045.2.
UniGeneMm.6393.

3D structure databases

ProteinModelPortalQ9QUI6.
SMRQ9QUI6. Positions 59-89.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000045134.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9QUI6.

Proteomic databases

PRIDEQ9QUI6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000038227; ENSMUSP00000045134; ENSMUSG00000037872.
GeneID13349.
KEGGmmu:13349.

Organism-specific databases

CTD2532.
MGIMGI:1097689. Darc.

Phylogenomic databases

eggNOGNOG28176.
GeneTreeENSGT00390000006372.
HOVERGENHBG051419.
InParanoidQ9QUI6.
KOK06574.
OrthoDBEOG7RFTHW.
PhylomeDBQ9QUI6.
TreeFamTF105419.

Gene expression databases

BgeeQ9QUI6.
CleanExMM_DARC.
GenevestigatorQ9QUI6.

Family and domain databases

InterProIPR005384. Duffy_chemokine_rcpt.
[Graphical view]
PANTHERPTHR14181. PTHR14181. 1 hit.
PRINTSPR01559. DUFFYANTIGEN.
ProtoNetSearch...

Other

NextBio283672.
PROQ9QUI6.
SOURCESearch...

Entry information

Entry nameACKR1_MOUSE
AccessionPrimary (citable) accession number: Q9QUI6
Secondary accession number(s): O35970 expand/collapse secondary AC list , Q91VB6, Q920X0, Q920X1, Q920X2, Q920X3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: January 10, 2003
Last modified: April 16, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries