Q9QUH6 (SYGP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras GTPase-activating protein SynGAP

Alternative name(s):
Neuronal RasGAP
Synaptic Ras GTPase-activating protein 1
Short name=Synaptic Ras-GAP 1
p135 SynGAP

Gene names

Name:

Syngap1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1308 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits. Ref.5 Ref.6 Ref.9
Subunit structure	Isoforms containing the PDZ-binding domain associate with DLG4 and DLG3 to form the PSD protein complex colocalized with GRIN2B at synapses. Interacts with MPDZ, KLHL17 CAMK2A and CAMK2B. Ref.3 Ref.5 Ref.7
Subcellular location	Membrane; Peripheral membrane protein. Cell junction › synapse. Note: Mostly in excitatory glutamatergic synapses.
Tissue specificity	Highly expressed in brain; predominantly in the cortex, hippocampus and olfactory bulb. Present in the postsynaptic density of central excitatory synapses. Ref.8
Domain	The PDZ-binding domain interacts with all three PDZ domains of DGL4.
Post-translational modification	Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption. Phosphorylation by PLK2 promotes its activity. Ref.5 Ref.10
Sequence similarities	Contains 1 C2 domain. Contains 1 PH domain. Contains 1 Ras-GAP domain.
Caution	It is uncertain whether Met-1 or Met-16 is the initiator methionine.
Sequence caution	The sequence AAC08071.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell junction Membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	SH3-binding
Molecular function	GTPase activation
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of Ras protein signal transduction Inferred from direct assay Ref.10. Source: UniProtKB regulation of synaptic plasticity Inferred from direct assay Ref.10. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from Biological aspect of Ancestor. Source: RefGenome intrinsic to internal side of plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome postsynaptic density Inferred from direct assay PubMed 14970204PubMed 21736925. Source: RGD
Molecular_function	Ras GTPase activator activity Inferred from direct assay PubMed 14970204PubMed 16427633Ref.10. Source: RGD phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9QUH6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9QUH6-2)

Also known as: SynGAP-a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.
     1296-1308: RGSFPPWVQQTRV → LLIR

Isoform 3 (identifier: Q9QUH6-3)

Also known as: SynGAP-b;

The sequence of this isoform differs from the canonical sequence as follows:
     1-98: MSRSRASIHR...PVEGRPHGEH → MGLRPPTPTP...RRCSSCCFPG

Isoform 4 (identifier: Q9QUH6-4)

Also known as: SynGAP-c;

The sequence of this isoform differs from the canonical sequence as follows:
     1-173: Missing.
     1296-1308: RGSFPPWVQQTRV → VERQLPPLGPTNPRVTLAPPWNGLAPPAPPPPPRLQITENGEFRNTADH

Isoform 5 (identifier: Q9QUH6-5)

Also known as: SynGAP-d; SynGAP-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: MSRSRASIHR...GGKQYSMEAA → MEYF
     1195-1196: Missing.
     1265-1308: RLMLVEEELRRDHPAMAEPLPEPKKRLLDAQRGSFPPWVQQTRV → SPSLQADAGGGGAAPGPPRHG

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1308

1308

Ras GTPase-activating protein SynGAP

PRO_0000056655

Regions

Domain

150 – 251

102

PH

Domain

249 – 347

99

C2

Domain

443 – 635

193

Ras-GAP

Region

1197 – 1308

112

Interaction with MPDZ

Motif

785 – 815

31

SH3-binding Potential

Motif

1305 – 1308

4

PDZ-binding Potential

Amino acid modifications

Modified residue

379

1

Phosphoserine; by PLK2 Ref.10

Modified residue

385

1

Phosphoserine; by PLK2 Ref.10

Modified residue

449

1

Phosphoserine; by PLK2 Ref.10

Modified residue

466

1

Phosphoserine; by PLK2 Ref.10

Modified residue

836

1

Phosphoserine; by PLK2 Ref.10

Modified residue

840

1

Phosphoserine; by PLK2 Ref.10

Modified residue

842

1

Phosphoserine; by PLK2 Ref.10

Modified residue

895

1

Phosphoserine; by PLK2 Ref.10

Natural variations

Alternative sequence

1 – 173

173

Missing in isoform 4.

VSP_007976

Alternative sequence

1 – 121

121

MSRSR…SMEAA → MEYF in isoform 5.

VSP_007975

Alternative sequence

1 – 98

98

MSRSR…PHGEH → MGLRPPTPTPSGGSGSGSLP PPSHRQPLRRRCSSCCFPG in isoform 3.

VSP_007974

Alternative sequence

1 – 15

15

Missing in isoform 2.

VSP_026378

Alternative sequence

1195 – 1196

2

Missing in isoform 5.

VSP_007977

Alternative sequence

1265 – 1308

44

RLMLV…QQTRV → SPSLQADAGGGGAAPGPPRH G in isoform 5.

VSP_007978

Alternative sequence

1296 – 1308

13

RGSFP…QQTRV → LLIR in isoform 2.

VSP_007979

Alternative sequence

1296 – 1308

13

RGSFP…QQTRV → VERQLPPLGPTNPRVTLAPP WNGLAPPAPPPPPRLQITEN GEFRNTADH in isoform 4.

VSP_007980

Experimental info

Mutagenesis

385

1

S → A: Affects stimulation by PLK2. Ref.10

Mutagenesis

449

1

S → A: Affects stimulation by PLK2. Ref.10

Mutagenesis

840

1

S → A: Blocks the gel mobility shift induced by PLK2 and affects stimulation by PLK2. Ref.10

Mutagenesis

842

1

S → A: Blocks the gel mobility shift induced by PLK2. Ref.10

Sequence conflict

308 – 309

2

WG → GY AA sequence Ref.1

Sequence conflict

316

1

N → M AA sequence Ref.1

Sequence conflict

427 – 429

3

HYR → GQK AA sequence Ref.1

Sequence conflict

937 – 939

3

DGP → ADG AA sequence Ref.1

Sequence conflict

1002

1

H → G AA sequence Ref.1

Sequence conflict

1088

1

S → Q AA sequence Ref.1

Sequence conflict

1277

1

H → L AA sequence Ref.1

Secondary structure

1

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1308

Helix Strand Turn

Details...

Beta strand

257 – 262

6

Beta strand

403 – 408

6

Helix

414 – 417

4

Helix

418 – 425

8

Helix

428 – 438

11

Helix

441 – 457

17

Helix

463 – 475

13

Beta strand

477 – 481

5

Beta strand

486 – 488

3

Helix

489 – 501

13

Helix

503 – 519

17

Turn

528 – 530

3

Turn

533 – 535

3

Helix

536 – 555

20

Helix

556 – 560

5

Helix

563 – 578

16

Helix

582 – 593

12

Turn

594 – 597

4

Helix

598 – 603

6

Turn

605 – 609

5

Helix

617 – 634

18

Turn

642 – 645

4

Helix

647 – 649

3

Helix

650 – 666

17

Helix

685 – 699

15

Helix

700 – 702

3

Helix

705 – 710

6

Turn

711 – 713

3

Helix

714 – 724

11

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: CA2536782C8C4DCB	FASTA	1,308	144,722
	10 20 30 40 50 60 MSRSRASIHR GSIPAMSYAP FRDVRGPPMH RTQYVHSPYD RPGWNPRFCI ISGNQLLMLD 70 80 90 100 110 120 EDEIHPLLIR DRRSESSRNK LLRRTVSVPV EGRPHGEHEY HLGRSRRKSV PGGKQYSMEA 130 140 150 160 170 180 APAAPFRPSQ GFLSRRLKSS IKRTKSQPKL DRTSSFRQIL PRFRSADHDR ARLMQSFKES 190 200 210 220 230 240 HSHESLLSPS SAAEALELNL DEDSIIKPVH SSILGQEFCF EVTTSSGTKC FACRSAAERD 250 260 270 280 290 300 KWIENLQRAV KPNKDNSRRV DNVLKLWIIE ARELPPKKRY YCELCLDDML YARTTSKPRS 310 320 330 340 350 360 ASGDTVFWGE HFEFNNLPAV RALRLHLYRD SDKKRKKDKA GYVGLVTVPV ATLAGRHFTE 370 380 390 400 410 420 QWYPVTLPTG SGGSGGMGSG GGGGSGGGSG GKGKGGCPAV RLKARYQTMS ILPMELYKEF 430 440 450 460 470 480 AEYVTNHYRM LCAVLEPALN VKGKEEVASA LVHILQSTGK AKDFLSDMAM SEVDRFMERE 490 500 510 520 530 540 HLIFRENTLA TKAIEEYMRL IGQKYLKDAI GEFIRALYES EENCEVDPIK CTASSLAEHQ 550 560 570 580 590 600 ANLRMCCELA LCKVVNSHCV FPRELKEVFA SWRLRCAERG REDIADRLIS ASLFLRFLCP 610 620 630 640 650 660 AIMSPSLFGL MQEYPDEQTS RTLTLIAKVI QNLANFSKFT SKEDFLGFMN EFLELEWGSM 670 680 690 700 710 720 QQFLYEISNL DTLTNSSSFE GYIDLGRELS TLHALLWEVL PQLSKEALLK LGPLPRLLSD 730 740 750 760 770 780 ISTALRNPNI QRQPSRQSER ARSQPMVLRG PSAEMQGYMM RDLNSSIDLQ SFMARGLNSS 790 800 810 820 830 840 MDMARLPSPT KEKPPPPPPG GGKDLFYVSR PPLARSSPAY CTSSSDITEP EQKMLSVNKS 850 860 870 880 890 900 VSMLDLQGDG PGGRLNSSSV SNLAAVGDLL HSSQASLTAA LGLRPAPAGR LSQGSGSSIT 910 920 930 940 950 960 AAGMRLSQMG VTTDGVPAQQ LRIPLSFQNP LFHMAADGPG PPAGHGGSSG HGPPSSHHHH 970 980 990 1000 1010 1020 HHHHHHRGGE PPGDTFAPFH GYSKSEDLST GVPKPPAASI LHSHSYSDEF GPSGTDFTRR 1030 1040 1050 1060 1070 1080 QLSLQDNLQH MLSPPQITIG PQRPAPSGPG GGSGGGSGGG GGGQPPPLQR GKSQQLTVSA 1090 1100 1110 1120 1130 1140 AQKPRPSSGN LLQSPEPSYG PARPRQQSLS KEGSIGGSGG SGGGGGGGLK PSITKQHSQT 1150 1160 1170 1180 1190 1200 PSTLNPTMPA SERTVAWVSN MPHLSADIES AHIEREEYKL KEYSKSMDES RLDRVKEYEE 1210 1220 1230 1240 1250 1260 EIHSLKERLH MSNRKLEEYE RRLLSQEEQT SKILMQYQAR LEQSEKRLRQ QQVEKDSQIK 1270 1280 1290 1300 SIIGRLMLVE EELRRDHPAM AEPLPEPKKR LLDAQRGSFP PWVQQTRV
	Isoform 2 (SynGAP-a) [UniParc]. Checksum: FA9F3DC3D874C0C8	FASTA	1,284	142,070
	10 20 30 40 50 60 MSYAPFRDVR GPPMHRTQYV HSPYDRPGWN PRFCIISGNQ LLMLDEDEIH PLLIRDRRSE 70 80 90 100 110 120 SSRNKLLRRT VSVPVEGRPH GEHEYHLGRS RRKSVPGGKQ YSMEAAPAAP FRPSQGFLSR 130 140 150 160 170 180 RLKSSIKRTK SQPKLDRTSS FRQILPRFRS ADHDRARLMQ SFKESHSHES LLSPSSAAEA 190 200 210 220 230 240 LELNLDEDSI IKPVHSSILG QEFCFEVTTS SGTKCFACRS AAERDKWIEN LQRAVKPNKD 250 260 270 280 290 300 NSRRVDNVLK LWIIEARELP PKKRYYCELC LDDMLYARTT SKPRSASGDT VFWGEHFEFN 310 320 330 340 350 360 NLPAVRALRL HLYRDSDKKR KKDKAGYVGL VTVPVATLAG RHFTEQWYPV TLPTGSGGSG 370 380 390 400 410 420 GMGSGGGGGS GGGSGGKGKG GCPAVRLKAR YQTMSILPME LYKEFAEYVT NHYRMLCAVL 430 440 450 460 470 480 EPALNVKGKE EVASALVHIL QSTGKAKDFL SDMAMSEVDR FMEREHLIFR ENTLATKAIE 490 500 510 520 530 540 EYMRLIGQKY LKDAIGEFIR ALYESEENCE VDPIKCTASS LAEHQANLRM CCELALCKVV 550 560 570 580 590 600 NSHCVFPREL KEVFASWRLR CAERGREDIA DRLISASLFL RFLCPAIMSP SLFGLMQEYP 610 620 630 640 650 660 DEQTSRTLTL IAKVIQNLAN FSKFTSKEDF LGFMNEFLEL EWGSMQQFLY EISNLDTLTN 670 680 690 700 710 720 SSSFEGYIDL GRELSTLHAL LWEVLPQLSK EALLKLGPLP RLLSDISTAL RNPNIQRQPS 730 740 750 760 770 780 RQSERARSQP MVLRGPSAEM QGYMMRDLNS SIDLQSFMAR GLNSSMDMAR LPSPTKEKPP 790 800 810 820 830 840 PPPPGGGKDL FYVSRPPLAR SSPAYCTSSS DITEPEQKML SVNKSVSMLD LQGDGPGGRL 850 860 870 880 890 900 NSSSVSNLAA VGDLLHSSQA SLTAALGLRP APAGRLSQGS GSSITAAGMR LSQMGVTTDG 910 920 930 940 950 960 VPAQQLRIPL SFQNPLFHMA ADGPGPPAGH GGSSGHGPPS SHHHHHHHHH HRGGEPPGDT 970 980 990 1000 1010 1020 FAPFHGYSKS EDLSTGVPKP PAASILHSHS YSDEFGPSGT DFTRRQLSLQ DNLQHMLSPP 1030 1040 1050 1060 1070 1080 QITIGPQRPA PSGPGGGSGG GSGGGGGGQP PPLQRGKSQQ LTVSAAQKPR PSSGNLLQSP 1090 1100 1110 1120 1130 1140 EPSYGPARPR QQSLSKEGSI GGSGGSGGGG GGGLKPSITK QHSQTPSTLN PTMPASERTV 1150 1160 1170 1180 1190 1200 AWVSNMPHLS ADIESAHIER EEYKLKEYSK SMDESRLDRV KEYEEEIHSL KERLHMSNRK 1210 1220 1230 1240 1250 1260 LEEYERRLLS QEEQTSKILM QYQARLEQSE KRLRQQQVEK DSQIKSIIGR LMLVEEELRR 1270 1280 DHPAMAEPLP EPKKRLLDAQ LLIR
	Isoform 3 (SynGAP-b) [UniParc]. Checksum: DC549FCF1624BD8A	FASTA	1,249	137,359
	10 20 30 40 50 60 MGLRPPTPTP SGGSGSGSLP PPSHRQPLRR RCSSCCFPGE YHLGRSRRKS VPGGKQYSME 70 80 90 100 110 120 AAPAAPFRPS QGFLSRRLKS SIKRTKSQPK LDRTSSFRQI LPRFRSADHD RARLMQSFKE 130 140 150 160 170 180 SHSHESLLSP SSAAEALELN LDEDSIIKPV HSSILGQEFC FEVTTSSGTK CFACRSAAER 190 200 210 220 230 240 DKWIENLQRA VKPNKDNSRR VDNVLKLWII EARELPPKKR YYCELCLDDM LYARTTSKPR 250 260 270 280 290 300 SASGDTVFWG EHFEFNNLPA VRALRLHLYR DSDKKRKKDK AGYVGLVTVP VATLAGRHFT 310 320 330 340 350 360 EQWYPVTLPT GSGGSGGMGS GGGGGSGGGS GGKGKGGCPA VRLKARYQTM SILPMELYKE 370 380 390 400 410 420 FAEYVTNHYR MLCAVLEPAL NVKGKEEVAS ALVHILQSTG KAKDFLSDMA MSEVDRFMER 430 440 450 460 470 480 EHLIFRENTL ATKAIEEYMR LIGQKYLKDA IGEFIRALYE SEENCEVDPI KCTASSLAEH 490 500 510 520 530 540 QANLRMCCEL ALCKVVNSHC VFPRELKEVF ASWRLRCAER GREDIADRLI SASLFLRFLC 550 560 570 580 590 600 PAIMSPSLFG LMQEYPDEQT SRTLTLIAKV IQNLANFSKF TSKEDFLGFM NEFLELEWGS 610 620 630 640 650 660 MQQFLYEISN LDTLTNSSSF EGYIDLGREL STLHALLWEV LPQLSKEALL KLGPLPRLLS 670 680 690 700 710 720 DISTALRNPN IQRQPSRQSE RARSQPMVLR GPSAEMQGYM MRDLNSSIDL QSFMARGLNS 730 740 750 760 770 780 SMDMARLPSP TKEKPPPPPP GGGKDLFYVS RPPLARSSPA YCTSSSDITE PEQKMLSVNK 790 800 810 820 830 840 SVSMLDLQGD GPGGRLNSSS VSNLAAVGDL LHSSQASLTA ALGLRPAPAG RLSQGSGSSI 850 860 870 880 890 900 TAAGMRLSQM GVTTDGVPAQ QLRIPLSFQN PLFHMAADGP GPPAGHGGSS GHGPPSSHHH 910 920 930 940 950 960 HHHHHHHRGG EPPGDTFAPF HGYSKSEDLS TGVPKPPAAS ILHSHSYSDE FGPSGTDFTR 970 980 990 1000 1010 1020 RQLSLQDNLQ HMLSPPQITI GPQRPAPSGP GGGSGGGSGG GGGGQPPPLQ RGKSQQLTVS 1030 1040 1050 1060 1070 1080 AAQKPRPSSG NLLQSPEPSY GPARPRQQSL SKEGSIGGSG GSGGGGGGGL KPSITKQHSQ 1090 1100 1110 1120 1130 1140 TPSTLNPTMP ASERTVAWVS NMPHLSADIE SAHIEREEYK LKEYSKSMDE SRLDRVKEYE 1150 1160 1170 1180 1190 1200 EEIHSLKERL HMSNRKLEEY ERRLLSQEEQ TSKILMQYQA RLEQSEKRLR QQQVEKDSQI 1210 1220 1230 1240 KSIIGRLMLV EEELRRDHPA MAEPLPEPKK RLLDAQRGSF PPWVQQTRV
	Isoform 4 (SynGAP-c) [UniParc]. Checksum: A4C6BF477EEF6A61	FASTA	1,171	128,489
	10 20 30 40 50 60 MQSFKESHSH ESLLSPSSAA EALELNLDED SIIKPVHSSI LGQEFCFEVT TSSGTKCFAC 70 80 90 100 110 120 RSAAERDKWI ENLQRAVKPN KDNSRRVDNV LKLWIIEARE LPPKKRYYCE LCLDDMLYAR 130 140 150 160 170 180 TTSKPRSASG DTVFWGEHFE FNNLPAVRAL RLHLYRDSDK KRKKDKAGYV GLVTVPVATL 190 200 210 220 230 240 AGRHFTEQWY PVTLPTGSGG SGGMGSGGGG GSGGGSGGKG KGGCPAVRLK ARYQTMSILP 250 260 270 280 290 300 MELYKEFAEY VTNHYRMLCA VLEPALNVKG KEEVASALVH ILQSTGKAKD FLSDMAMSEV 310 320 330 340 350 360 DRFMEREHLI FRENTLATKA IEEYMRLIGQ KYLKDAIGEF IRALYESEEN CEVDPIKCTA 370 380 390 400 410 420 SSLAEHQANL RMCCELALCK VVNSHCVFPR ELKEVFASWR LRCAERGRED IADRLISASL 430 440 450 460 470 480 FLRFLCPAIM SPSLFGLMQE YPDEQTSRTL TLIAKVIQNL ANFSKFTSKE DFLGFMNEFL 490 500 510 520 530 540 ELEWGSMQQF LYEISNLDTL TNSSSFEGYI DLGRELSTLH ALLWEVLPQL SKEALLKLGP 550 560 570 580 590 600 LPRLLSDIST ALRNPNIQRQ PSRQSERARS QPMVLRGPSA EMQGYMMRDL NSSIDLQSFM 610 620 630 640 650 660 ARGLNSSMDM ARLPSPTKEK PPPPPPGGGK DLFYVSRPPL ARSSPAYCTS SSDITEPEQK 670 680 690 700 710 720 MLSVNKSVSM LDLQGDGPGG RLNSSSVSNL AAVGDLLHSS QASLTAALGL RPAPAGRLSQ 730 740 750 760 770 780 GSGSSITAAG MRLSQMGVTT DGVPAQQLRI PLSFQNPLFH MAADGPGPPA GHGGSSGHGP 790 800 810 820 830 840 PSSHHHHHHH HHHRGGEPPG DTFAPFHGYS KSEDLSTGVP KPPAASILHS HSYSDEFGPS 850 860 870 880 890 900 GTDFTRRQLS LQDNLQHMLS PPQITIGPQR PAPSGPGGGS GGGSGGGGGG QPPPLQRGKS 910 920 930 940 950 960 QQLTVSAAQK PRPSSGNLLQ SPEPSYGPAR PRQQSLSKEG SIGGSGGSGG GGGGGLKPSI 970 980 990 1000 1010 1020 TKQHSQTPST LNPTMPASER TVAWVSNMPH LSADIESAHI EREEYKLKEY SKSMDESRLD 1030 1040 1050 1060 1070 1080 RVKEYEEEIH SLKERLHMSN RKLEEYERRL LSQEEQTSKI LMQYQARLEQ SEKRLRQQQV 1090 1100 1110 1120 1130 1140 EKDSQIKSII GRLMLVEEEL RRDHPAMAEP LPEPKKRLLD AQVERQLPPL GPTNPRVTLA 1150 1160 1170 PPWNGLAPPA PPPPPRLQIT ENGEFRNTAD H
	Isoform 5 (SynGAP-d) (SynGAP-beta) [UniParc]. Checksum: 5804EE4163AE4950	FASTA	1,166	127,745
	10 20 30 40 50 60 MEYFPAAPFR PSQGFLSRRL KSSIKRTKSQ PKLDRTSSFR QILPRFRSAD HDRARLMQSF 70 80 90 100 110 120 KESHSHESLL SPSSAAEALE LNLDEDSIIK PVHSSILGQE FCFEVTTSSG TKCFACRSAA 130 140 150 160 170 180 ERDKWIENLQ RAVKPNKDNS RRVDNVLKLW IIEARELPPK KRYYCELCLD DMLYARTTSK 190 200 210 220 230 240 PRSASGDTVF WGEHFEFNNL PAVRALRLHL YRDSDKKRKK DKAGYVGLVT VPVATLAGRH 250 260 270 280 290 300 FTEQWYPVTL PTGSGGSGGM GSGGGGGSGG GSGGKGKGGC PAVRLKARYQ TMSILPMELY 310 320 330 340 350 360 KEFAEYVTNH YRMLCAVLEP ALNVKGKEEV ASALVHILQS TGKAKDFLSD MAMSEVDRFM 370 380 390 400 410 420 EREHLIFREN TLATKAIEEY MRLIGQKYLK DAIGEFIRAL YESEENCEVD PIKCTASSLA 430 440 450 460 470 480 EHQANLRMCC ELALCKVVNS HCVFPRELKE VFASWRLRCA ERGREDIADR LISASLFLRF 490 500 510 520 530 540 LCPAIMSPSL FGLMQEYPDE QTSRTLTLIA KVIQNLANFS KFTSKEDFLG FMNEFLELEW 550 560 570 580 590 600 GSMQQFLYEI SNLDTLTNSS SFEGYIDLGR ELSTLHALLW EVLPQLSKEA LLKLGPLPRL 610 620 630 640 650 660 LSDISTALRN PNIQRQPSRQ SERARSQPMV LRGPSAEMQG YMMRDLNSSI DLQSFMARGL 670 680 690 700 710 720 NSSMDMARLP SPTKEKPPPP PPGGGKDLFY VSRPPLARSS PAYCTSSSDI TEPEQKMLSV 730 740 750 760 770 780 NKSVSMLDLQ GDGPGGRLNS SSVSNLAAVG DLLHSSQASL TAALGLRPAP AGRLSQGSGS 790 800 810 820 830 840 SITAAGMRLS QMGVTTDGVP AQQLRIPLSF QNPLFHMAAD GPGPPAGHGG SSGHGPPSSH 850 860 870 880 890 900 HHHHHHHHHR GGEPPGDTFA PFHGYSKSED LSTGVPKPPA ASILHSHSYS DEFGPSGTDF 910 920 930 940 950 960 TRRQLSLQDN LQHMLSPPQI TIGPQRPAPS GPGGGSGGGS GGGGGGQPPP LQRGKSQQLT 970 980 990 1000 1010 1020 VSAAQKPRPS SGNLLQSPEP SYGPARPRQQ SLSKEGSIGG SGGSGGGGGG GLKPSITKQH 1030 1040 1050 1060 1070 1080 SQTPSTLNPT MPASERTVAW VSNMPHLSAD IESAHIEREE YKLKEYSKSM DESRLDREYE 1090 1100 1110 1120 1130 1140 EEIHSLKERL HMSNRKLEEY ERRLLSQEEQ TSKILMQYQA RLEQSEKRLR QQQVEKDSQI 1150 1160 KSIIGSPSLQ ADAGGGGAAP GPPRHG

References

[1]	"A synaptic Ras-GTPase activating protein (p135 SynGAP) inhibited by CaM kinase II." Chen H.-J., Rojas-Soto M., Oguni A., Kennedy M.B. Neuron 20:895-904(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 16-276 (ISOFORM 3), NUCLEOTIDE SEQUENCE [MRNA] OF 1264-1290 (ISOFORM 5), PROTEIN SEQUENCE OF 32-47; 242-248; 259-272; 300-321; 325-329; 340-354; 419-429; 588-596; 804-815; 923-940; 968-1002; 1086-1102 AND 1276-1286. Strain: Sprague-Dawley.
[2]	Erratum Oh J.S., Chen H.-J., Rojas-Soto M., Oguni A., Kennedy M.B. Neuron 33:151-151(2002)
[3]	"SynGAP: a synaptic RasGAP that associates with the PSD-95/SAP90 protein family." Kim J.H., Liao D., Lau L.-F., Huganir R.L. Neuron 20:683-691(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2; 3 AND 4), INTERACTION WITH DLG3 AND DLG4. Tissue: Hippocampus.
[4]	"Characterization of a novel synGAP isoform, synGAP-beta." Li W., Okano A., Tian Q.B., Nakayama K., Furihata T., Nawa H., Suzuki T. J. Biol. Chem. 276:21417-21424(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5). Strain: Sprague-Dawley.
[5]	"SynGAP-MUPP1-CaMKII synaptic complexes regulate p38 MAP kinase activity and NMDA receptor-dependent synaptic AMPA receptor potentiation." Krapivinsky G., Medina I., Krapivinsky L., Gapon S., Clapham D.E. Neuron 43:563-574(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MPDZ; DLG4; CAMK2A AND CAMK2B, PHOSPHORYLATION, FUNCTION.
[6]	"Impaired SynGAP expression and long-term spatial learning and memory in hippocampal CA1 area from rats previously exposed to perinatal hypoxia-induced insults: beneficial effects of A68930." Yang S.-N., Huang C.-B., Yang C.-H., Lai M.-C., Chen W.-F., Wang C.-L., Wu C.-L., Huang L.-T. Neurosci. Lett. 371:73-78(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"Interactions between CAP70 and actinfilin are important for integrity of actin cytoskeleton structures in neurons." Chen Y., Li M. Neuropharmacology 49:1026-1041(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH KLHL17.
[8]	"Relative and absolute quantification of postsynaptic density proteome isolated from rat forebrain and cerebellum." Cheng D., Hoogenraad C.C., Rush J., Ramm E., Schlager M.A., Duong D.M., Xu P., Wijayawardana S.R., Hanfelt J., Nakagawa T., Sheng M., Peng J. Mol. Cell. Proteomics 5:1158-1170(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[9]	"SynGAP regulates synaptic strength and mitogen-activated protein kinases in cultured neurons." Rumbaugh G., Adams J.P., Kim J.H., Huganir R.L. Proc. Natl. Acad. Sci. U.S.A. 103:4344-4351(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"Requirement for Plk2 in orchestrated ras and rap signaling, homeostatic structural plasticity, and memory." Lee K.J., Lee Y., Rozeboom A., Lee J.Y., Udagawa N., Hoe H.S., Pak D.T. Neuron 69:957-973(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-379; SER-385; SER-449; SER-466; SER-836; SER-840; SER-842 AND SER-895, MUTAGENESIS OF SER-385; SER-449; SER-840 AND SER-842.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF048976 mRNA. Translation: AAC08071.1. Different initiation.
AF053938 mRNA. Translation: AAC23491.1.
AF055883 mRNA. Translation: AAC23492.1.
AF058789 mRNA. Translation: AAC63510.2.
AF058790 mRNA. Translation: AAC63511.1.
AF050183 mRNA. Translation: AAC40082.2.
AB016962 mRNA. Translation: BAA74972.1.

IPI

IPI00212566.
IPI00339023.
IPI00339026.
IPI00339027.
IPI00391507.

PIR

T13958.
T14259.
T14270.

UniGene

Rn.9908.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3BXJ	X-ray	3.00	A/B	252-734	[»]

ProteinModelPortal

Q9QUH6.

SMR

Q9QUH6. Positions 401-734.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9QUH6. 1 interaction.

MINT

MINT-1778299.

PTM databases

PhosphoSite

Q9QUH6.

Proteomic databases

PaxDb

Q9QUH6.

PRIDE

Q9QUH6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

UCSC

RGD:621090. rat.

Organism-specific databases

RGD

621090. Syngap1.

Phylogenomic databases

eggNOG

NOG245428.

HOVERGEN

HBG006492.

InParanoid

Q9QUH6.

OrthoDB

EOG49ZXNH.

Gene expression databases

ArrayExpress

Q9QUH6.

Genevestigator

Q9QUH6.

GermOnline

ENSRNOG00000000483. Rattus norvegicus.

Family and domain databases

Gene3D

1.10.506.10. 1 hit.
2.20.170.10. 1 hit.
2.30.29.30. 2 hits.

InterPro

IPR000008. C2_Ca-dep.
IPR008973. C2_Ca/lipid-bd_dom_CaLB.
IPR021887. DUF3498.
IPR011993. PH_like_dom.
IPR001849. Pleckstrin_homology.
IPR001936. RasGAP.
IPR023152. RasGAP_CS.
IPR008936. Rho_GTPase_activation_prot.
IPR023315. SynGAP_C2_N.
[Graphical view]

Pfam

PF00168. C2. 1 hit.
PF12004. DUF3498. 1 hit.
PF00616. RasGAP. 1 hit.
[Graphical view]

SMART

SM00239. C2. 1 hit.
SM00233. PH. 1 hit.
SM00323. RasGAP. 1 hit.
[Graphical view]

SUPFAM

SSF49562. C2_CaLB. 1 hit.
SSF48350. Rho_GAP. 1 hit.

PROSITE

PS50004. C2. False negative.
PS50003. PH_DOMAIN. 1 hit.
PS00509. RAS_GTPASE_ACTIV_1. 1 hit.
PS50018. RAS_GTPASE_ACTIV_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q9QUH6.

Entry information

Entry name

SYGP1_RAT

Accession

Primary (citable) accession number: Q9QUH6
Secondary accession number(s): O88449 , Q9ESK6, Q9ET81, Q9QX02, Q9QX06, Q9QX12

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	June 26, 2007
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program