Q9QTF2 (RIR2_RSIV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase small chain
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase small subunit

Gene names

Name:

RR-2

Organism

Red sea bream iridovirus (RSIV)

Taxonomic identifier

65424 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Iridoviridae › Megalocytivirus › unclassified Megalocytivirus

Virus host

Epinephelus [TaxID: 94231]
Lateolabrax [TaxID: 8163]
Seriola [TaxID: 8160]
Thunnus thynnus (Atlantic bluefin tuna) (Scomber thynnus) [TaxID: 8237]

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small chain By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Ribonucleoside-diphosphate reductase small chain		PRO_0000190493
Sites
	Active site	101	1	By similarity
	Metal binding	62	1	Iron 1 By similarity
	Metal binding	94	1	Iron 1 By similarity
	Metal binding	94	1	Iron 2 By similarity
	Metal binding	97	1	Iron 1 By similarity
	Metal binding	156	1	Iron 2 By similarity
	Metal binding	190	1	Iron 2 By similarity
	Metal binding	193	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QTF2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7D3E43D5F4F48B0B	FASTA	312	35,653
	10 20 30 40 50 60 MDRYVLRPND PHLWAMYKKA VASFWTVEEV DLSTDVRHWT DKLTEAERRF MSHILAFFAM 70 80 90 100 110 120 ADGIVGDNLV CNFAKELDHI PEARAFYGFQ VAIENIHAEM YTQLIITLVA KENQAALFSA 130 140 150 160 170 180 AEDMPCVKAK AQWAAQWLNA THKPIATRLL AFAAVEGVMF SGSFAAIFWL KKRSLMPGLA 190 200 210 220 230 240 FSNELISRDE GLHCDFACML FRQMANKPCQ DDAHQIISDA VDIETAFFRE ALKTPLLGMN 250 260 270 280 290 300 SDSMKLYIQF VADRLLDALG YAKMYNVSNP FDFMDNISIE GKTNFFERRV SEYQRMGVFT 310 PSSMEFTMNE EF

References

[1]

"7.1kbp DNA sequence of red sea bream iridovirus (RSIV) containing largest subunit of the DNA-dependent RNA polymerase (RPO-1) gene, RAD2 homolog gene and ribonucleotide reductase small subunit (RR-2) gene." Kurita J., Nakajima K., Hirono I., Aoki T. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Ehime-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB018418 Genomic DNA. Translation: BAA82755.1.
3D structure databases
ProteinModelPortal	Q9QTF2.
SMR	Q9QTF2. Positions 2-275.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_RSIV

Accession

Primary (citable) accession number: Q9QTF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program