Q9QSP4 (NCAP_ABLVB) Reviewed, UniProtKB/Swiss-Prot
Last modified April 3, 2013. Version 40. History...
Names and origin
|Protein names||Recommended name:|
Short name=Protein N
|Organism||Australian bat lyssavirus (isolate Bat/AUS/1996) (ABLV) [Reference proteome]|
|Taxonomic identifier||446561 [NCBI]|
|Taxonomic lineage||Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus ›|
|Virus host||Homo sapiens (Human) [TaxID: 9606]|
Pteropus alecto (Black flying fox) [TaxID: 9402]
Pteropus conspicillatus (Spectacled flying fox) [TaxID: 328804]
Pteropus poliocephalus (Grey-headed flying fox) [TaxID: 9403]
Pteropus scapulatus (Little red flying fox) [TaxID: 94117]
Saccolaimus [TaxID: 446909]
|Sequence length||450 AA.|
|Protein existence||Inferred from homology|
General annotation (Comments)
Encapsidates the genome in a ratio of one protein N per nine ribonucleotides, protecting it from nucleases. If expressed without protein P it binds non-specifically RNA and therefore can bind it's own mRNA. Interaction with protein P abolishes any non-specific RNA binding, and prevents phosphorylation. The soluble N-P complex encapsidates specifically the genomic RNA, with protein N protecting the genome like a pearl necklace. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for viral transcription and replication. Protein N binds protein P in the NC through a different interaction, and can be phosphorylated. Subsequent viral replication is dependent on intracellular concentration of newly synthesized protein N. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases By similarity.
Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. In nucleocapsid, binds protein P and thereby positions the polymerase on the template. Protein P acts as a chaperone on free protein N to prevent it from aggregation before encapsidating genomic RNA By similarity.
Phosphorylated by host CK2. Unphosphorylated protein N seems to have a better affinity for leader viral promoter encapsidation. Phosphorylation of protein N in ribonucleocapsid may stabilize the interaction with protein P, thereby playing an important role in viral transcription/replication By similarity.
Displays a superantigen activity in human and mouse, activating mostly V-beta-8 subtypes of T-cell receptor By similarity.
Belongs to the lyssavirus nucleocapsid protein family.
|Cellular component||Host cytoplasm|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Cellular_component||host cell cytoplasm|
Inferred from electronic annotation. Source: UniProtKB-SubCellribonucleoprotein complex
Inferred from electronic annotation. Source: UniProtKB-KWviral nucleocapsid
Inferred from electronic annotation. Source: InterPro
Inferred from electronic annotation. Source: UniProtKB-KW
|Complete GO annotation...|
Sequence annotation (Features)
|AF081020 Genomic RNA. Translation: AAD47896.1.|
|RefSeq||NP_478339.1. NC_003243.1. |
3D structure databases
|SMR||Q9QSP4. Positions 5-448. |
Protocols and materials databases
Genome annotation databases
Family and domain databases
|Gene3D||1.10.3570.10. 1 hit. |
1.10.3610.10. 1 hit.
|InterPro||IPR000448. Rhabdo_ncapsid. |
|Pfam||PF00945. Rhabdo_ncap. 1 hit. |
|ProDom||PD002087. Rhabd_nucleocap. 1 hit. |
[Graphical view] [Entries sharing at least one domain]
|SUPFAM||SSF140809. SSF140809. 1 hit. |
|Accession||Primary (citable) accession number: Q9QSP4|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|
Index of protein domains and families