Reviewed, UniProtKB/Swiss-Prot Q9QSP0 (L_ABLVB)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Australian bat lyssavirus (isolate Bat/AUS/1996) (ABLV) [Complete proteome]

Taxonomic identifier

446561 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus

Virus host

Pteropus alecto (Black flying fox) [TaxID: 9402]
Pteropus poliocephalus (Grey-headed flying fox) [TaxID: 9403]
Homo sapiens (Human) [TaxID: 9606]
Pteropus scapulatus (Little red flying fox) [TaxID: 94117]
Pteropus conspicillatus (Spectacled flying fox) [TaxID: 328804]
Saccolaimus [TaxID: 446909]

Protein attributes

Sequence length	2128 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-7-N-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein to form the functional polymerase By similarity.
Subcellular location	Virion. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviruses protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2128	2128	Large structural protein		PRO_0000294415
Regions
	Domain	612 – 800	189	RdRp catalytic
	Region	1563 – 2128	566	Interaction with P protein By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QSP0-1 [UniParc]. Last modified March 1, 2002. Version 2. Checksum: C7AB91F58AF2B948	FASTA	2,128	242,984
	10 20 30 40 50 60 MIDPGEVYDD PIDPVEPEPE LKTNNAVIPN ILRNSDYNLN SPLIEHPSRL MLEWLTTGNK 70 80 90 100 110 120 PMRLTLTDNC IRSYKTLKCY FRKVDIGSVK VGGPAAQAMT SLWLHGEHSE SNRSRKCLSD 130 140 150 160 170 180 LTQFYQKSSP IEKLLNYTLG NRGLRIPPEG VLLCLKKVDY DRSFGRYLAN IYSSYLFFHV 190 200 210 220 230 240 IILYMNALDW DEEKTILALW RDLNSVDIKK DQVKFRDSIW GSLIVTKEFV YSQNSNCLFD 250 260 270 280 290 300 RNYTLMLKDL FLSRFNSLLI LLSPPEPRYS DDLISLLCQL YIAGDHVLSM CGNSGYDVIK 310 320 330 340 350 360 MLEPYVINSL VQRAEEFRPL IHSLGDFPLF IREKVGQLEG TFGPSARRFF QVLDQVDNIH 370 380 390 400 410 420 DLVFVYGCYR HWGHPYIDYR KGLLKLYDQV HVKKMIDGAY QECLASDLAK RILRWGFDKY 430 440 450 460 470 480 SKWYLDPKLL APDHPLAPYI KTQTWPPKHI VDLVGNTWHK LPITQIFEIP ESMDPSEILD 490 500 510 520 530 540 DKSHSFTRTK LASWLAENRG GPVPSEKVII TALSKPPVNP REFLKSIDLG GLPDDDLIIG 550 560 570 580 590 600 LKPKERELKI EGRFFALMSW NLRLYFVITE KLLANYILPL FDALTMTDNL NKVFKKLIDR 610 620 630 640 650 660 VTGQGLQDYS RVTYAFHLDY EKWNNHQRLE STKDVFPVLD QVFGLKKVFS RTHEFFQKSW 670 680 690 700 710 720 IYYSDRSDLI GLWEDQIYCL DMSDGPTCWN GQDGGLEGLR QKGWSLVSLL MIDRESQTRN 730 740 750 760 770 780 TRTKDFAQGD NQVLCPTYML SSGLSHEGLL YELESISRNA LSIYRAIEDG ASKLGLIIKK 790 800 810 820 830 840 EETMCSYDFL IYGKTPLFRG NILVPESKRW ARVSCISNDQ IVNLANIMST VSTNALTVAQ 850 860 870 880 890 900 HSQSLIKPMR GFLLMAVQAV FHYLLFSPIL KGRVYKILSA EGDDFLLAMS RIVYLDPSLG 910 920 930 940 950 960 GVSGMSLGRF HIRQFSDPVS EGLSFWKEIW TSSSESWIHS LCQEAGNPDL GDRSLEKFTR 970 980 990 1000 1010 1020 LLEDPTTLNI RGGASPTILL KDAIRKALYD EVDKVENSEF REAILLSRHH RDNFILFLKS 1030 1040 1050 1060 1070 1080 IEPLFPRFLS ELFSSSFLGI PESIIGLIQN SRTIRRQFRR SLSRSLEESF YNSEIHGISR 1090 1100 1110 1120 1130 1140 MTQVPQRIGR VWSCSSERAH LLREISWGRK RVGTTVPHPS EMLALLPKSS ISCPCGLTGS 1150 1160 1170 1180 1190 1200 ENPRVSISVL PSIYQSFFSR GPLKGYLGSS TSMSTQTFHA WEKVTNVHVV KRALSLKESI 1210 1220 1230 1240 1250 1260 NWFISRNSNL AQTLIRNIIS LTGPQFPLEE APVFKRTGSA LHRFKSARYS EGGYSSICPN 1270 1280 1290 1300 1310 1320 LLSHISVSTD TMSDLTHDGR NFDFMFQPLM LYAQTWTSEL VQKDIRLKDS TFHWHLRCPK 1330 1340 1350 1360 1370 1380 CIRSIDDVIL VHPQVFDFPD VSKRISRMVS GAVPSSQILP EVNLLPGHFE SLCGRDKSRH 1390 1400 1410 1420 1430 1440 IGSAQGLLYS ILVATHDPGY NDGTIFPVNI YSKISPKDYL RGLARGILIG SSICLLTRMT 1450 1460 1470 1480 1490 1500 NININRPLEL ISGVITYILL RLDNHPSLYV MLREPSLRSE IFSIPQKIPA AYPTTMKEGN 1510 1520 1530 1540 1550 1560 RSVLCYLQHV LRYEREIITS SPENDWLWIF SDFRSMKMTY LTLITYQSHI FLQRIERSLS 1570 1580 1590 1600 1610 1620 KKMRADLRQL SSLMRQVLGG HGEDTLDSGE DIQRLLRDAI QRTKWVDQEV RHAAKTMTND 1630 1640 1650 1660 1670 1680 HSPSKKTSRK VGCSEWICSA QQVSISTSSN PAPVSEMDVR TLSRKLQNPL ISGLRVVQCA 1690 1700 1710 1720 1730 1740 TGAHYKLKPI LDNLNTYPSF CLVVGDGSGG ISRTVLNMFP DAKLVFNSLL EVSDLMASGT 1750 1760 1770 1780 1790 1800 HPLPPSAIMS GGEDITSRVI DFESIWEKPS DLRNLSTWRY FQSVQSQLNM SYDLIICDAE 1810 1820 1830 1840 1850 1860 VTDIASVNKI TLLMSDFVLS IDGPVDLIFK SYGSMLVDPD YKAIQHLSRA FPQVTGYITQ 1870 1880 1890 1900 1910 1920 LTSSFSSELY LRFSKRGKFF RDAEYLTSST LREMSLVLFN CSSPKSELQR ARSLNYQDLV 1930 1940 1950 1960 1970 1980 RGFPEEIVSN PYNEMIITLI DSEVESFLVH KMVDDLELQR GTLSRMSIII AIVIVYSNRV 1990 2000 2010 2020 2030 2040 FNVSKPLNDP LFYPPSDPKI LRHFNICCST MMYLSTVLGD VPNFARQHEL YNGPITYYFK 2050 2060 2070 2080 2090 2100 RQIIRGSIYL SWSWSDDTSV FKRVSCNSNL SLSSHWIRLI YKIVKTTRLT GSPVDLSKEV 2110 2120 EKHLRGYNRW ITLNDVKSRS SLLDYSCL

References

[1]	"Characterisation of an Australian bat lyssavirus variant isolated from an insectivorous bat." Gould A.R., Kattenbelt J.A., Gumley S.G., Lunt R.A. Virus Res. 89:1-28(2002) [PubMed: 12367747] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF081020 Genomic RNA. Translation: AAD47900.2.
RefSeq	NP_478343.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	926729.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR017234. RNA-dir_pol_rhabdovirus. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_ABLVB

Accession

Primary (citable) accession number: Q9QSP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	March 1, 2002
Last modified:	January 19, 2010
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)