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Q9QSK1 (TOP2_IIV6) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 2

EC=5.99.1.3
Alternative name(s):
DNA topoisomerase II
Gene names
Name:TOP2
ORF Names:IIV6-045L
OrganismInvertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus) [Reference proteome]
Taxonomic identifier176652 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageIridoviridaeIridovirus
Virus hostAcheta domesticus (House cricket) [TaxID: 6997]
Chilo suppressalis (striped riceborer) [TaxID: 168631]
Gryllus bimaculatus (Two-spotted cricket) [TaxID: 6999]
Gryllus campestris [TaxID: 58607]
Spodoptera frugiperda (Fall armyworm) [TaxID: 7108]

Protein attributes

Sequence length1132 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Can introduce negative superhelical turns into double-stranded circular DNA.

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA.

Cofactor

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity.

Sequence similarities

Belongs to the type II topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11321132DNA topoisomerase 2
PRO_0000377771

Regions

Domain442 – 577136Toprim
Nucleotide binding137 – 1393ATP By similarity
Nucleotide binding150 – 1578ATP By similarity
Nucleotide binding363 – 3653ATP By similarity

Sites

Active site8031O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding4481Magnesium 1; catalytic By similarity
Metal binding5381Magnesium 1; catalytic By similarity
Metal binding5381Magnesium 2 By similarity
Metal binding5401Magnesium 2 By similarity
Binding site681ATP By similarity
Binding site1001ATP By similarity
Site8021Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9QSK1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 4626121A5FCC6201

FASTA1,132129,877
        10         20         30         40         50         60 
MATSNHKKQY TTLSDIDHVL LRPEVIIGST VPMEQTEYVV DENFSEILEK QVFVSEALIR 

        70         80         90        100        110        120 
IFVEVLANAV DNIHRSKGSS TPCKSIKIKI EDDGTTMIHN DGQVIKISKE ENDGIEINGK 

       130        140        150        160        170        180 
KCSVYNHELV FGHLRSSSNY DDTVVRQTSG KNGLGVKCTN ILSKSFKVIG VDPENKKKFV 

       190        200        210        220        230        240 
QEWTNNMKET SGPKVTTSSS KTGYTEVIYC PDFERLGNFS SDIIGIFRKH VLDVAMLASS 

       250        260        270        280        290        300 
EGVSVYFNDK KLPIKKFSDY TKLFKLEKES ISITEEDGSS EVTIAFVERD GKKKPISFVN 

       310        320        330        340        350        360 
GLFTKHGGQH VDGWTKPFFA NLLSVLNKPS KTYKDLKLSL KDVTPYFRFF VKSTVDKPQF 

       370        380        390        400        410        420 
DCQNKNMLKS PCLKYTIPDK IITKVCKWTS VQELKQSSFE KLLKGKDNNI IKTLNDKKRR 

       430        440        450        460        470        480 
PKVSVKEYDP ANKAGTTLSE KCTLIVCEGL SAKTYAVAGI STGISFTGTD TKKGRDWFGI 

       490        500        510        520        530        540 
LPLRGKFLNV RNANNDKMIK NTVVTDLVNA MGLTFGMDYS TQKSRKSLNY GSIIIIPDPD 

       550        560        570        580        590        600 
EDGIHIEGLV LNFFHHHFSS LFNPVVPNLK DFPFISSMKI PIIKVIEKPL KGRREIEFFT 

       610        620        630        640        650        660 
HEAFHKFKNE NKWHKNHEIK YFKGLGTTKH EDVPKIFGKK MVVFNIDDNA HENMEKAFKD 

       670        680        690        700        710        720 
EEADERKEWL KQYNPNARTF DLDSPNKILQ LNISTFINEE LIKFSIEDCK RSLPHLIDGL 

       730        740        750        760        770        780 
KESQRKVIFG LKQWNGKNNI KVAQLGAFVA QKTDYKHGEQ NLFDTIIKMA QTFVGANNIP 

       790        800        810        820        830        840 
LLEEDGQFGT RLSGGKDAAS PRYIFVNQPP ILNKIFRPED DPILNYTLDG EPVFYAPVIP 

       850        860        870        880        890        900 
LICINGSVGV GTGFSCNIPM FNPSEIIDGL KQWINMREQG EKYVFKYKPW YKGFTGKIEK 

       910        920        930        940        950        960 
NGSGRYISYG TLSNEASNNG MYQYTVSELP INMWTDKFKE MCDQYRESNL IKTCDNYSEV 

       970        980        990       1000       1010       1020 
TTVNFKISSE HELTIENLKL RSYIHTSNMV MFDHEGKIHK YETLNGIMQR FCKERIKIYE 

      1030       1040       1050       1060       1070       1080 
KRRQYMLQKL NKSLTISENK KRFMEDVMNQ KIKILLQEDE VVHQQLLDMN YYKDDEGEFD 

      1090       1100       1110       1120       1130 
YLLNMNIGGF RKKNVDKLIS KIDDLKKDIM WYTNTNEGQM WLKDILELEP FI 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus."
Jakob N.J., Mueller K., Bahr U., Darai G.
Virology 286:182-196(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes."
Eaton H.E., Metcalf J., Penny E., Tcherepanov V., Upton C., Brunetti C.R.
Virol. J. 4:11-11(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF303741 Genomic DNA. Translation: AAD48151.1.
RefSeqNP_149508.1. NC_003038.1.

3D structure databases

ProteinModelPortalQ9QSK1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID921682.

Family and domain databases

Gene3D1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProIPR024946. Arg_repress_C-like.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR006171. Toprim_domain.
[Graphical view]
PfamPF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEPS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP2_IIV6
AccessionPrimary (citable) accession number: Q9QSK1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families