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Protein

DNA topoisomerase 2

Gene

TOP2

Organism
Invertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Can introduce negative superhelical turns into double-stranded circular DNA.

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.PROSITE-ProRule annotation

Cofactori

Mg2+PROSITE-ProRule annotation, Mn2+PROSITE-ProRule annotation, Ca2+PROSITE-ProRule annotationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68ATPBy similarity1
Binding sitei100ATPBy similarity1
Metal bindingi448Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi538Magnesium 1; catalyticPROSITE-ProRule annotation1
Metal bindingi538Magnesium 2PROSITE-ProRule annotation1
Metal bindingi540Magnesium 2PROSITE-ProRule annotation1
Sitei802Transition state stabilizerBy similarity1
Active sitei803O-(5'-phospho-DNA)-tyrosine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 139ATPBy similarity3
Nucleotide bindingi150 – 157ATPBy similarity8
Nucleotide bindingi363 – 365ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 2 (EC:5.99.1.3)
Alternative name(s):
DNA topoisomerase II
Gene namesi
Name:TOP2
ORF Names:IIV6-045L
OrganismiInvertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Taxonomic identifieri176652 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeBetairidovirinaeIridovirus
Virus hostiAcheta domesticus (House cricket) [TaxID: 6997]
Chilo suppressalis (Asiatic rice borer moth) [TaxID: 168631]
Gryllus bimaculatus (Two-spotted cricket) [TaxID: 6999]
Gryllus campestris [TaxID: 58607]
Spodoptera frugiperda (Fall armyworm) [TaxID: 7108]
Proteomesi
  • UP000001359 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003777711 – 1132DNA topoisomerase 2Add BLAST1132

Proteomic databases

PRIDEiQ9QSK1

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei486Interaction with DNAPROSITE-ProRule annotation1
Sitei489Interaction with DNABy similarity1
Sitei666Interaction with DNABy similarity1
Sitei667Interaction with DNABy similarity1
Sitei721Interaction with DNABy similarity1
Sitei755Interaction with DNABy similarity1
Sitei934Interaction with DNABy similarity1

Structurei

3D structure databases

ProteinModelPortaliQ9QSK1
SMRiQ9QSK1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini442 – 577ToprimPROSITE-ProRule annotationAdd BLAST136

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni327 – 329Interaction with DNABy similarity3
Regioni979 – 988Interaction with DNABy similarity10

Sequence similaritiesi

Belongs to the type II topoisomerase family.Curated

Phylogenomic databases

OrthoDBiVOG0900004O

Family and domain databases

Gene3Di1.10.268.10, 1 hit
3.30.230.10, 1 hit
3.30.565.10, 1 hit
3.40.50.670, 2 hits
3.90.199.10, 3 hits
InterProiView protein in InterPro
IPR036890 HATPase_C_sf
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
IPR001241 Topo_IIA
IPR013760 Topo_IIA-like_dom_sf
IPR002205 Topo_IIA_A/C
IPR013758 Topo_IIA_A/C_ab
IPR013757 Topo_IIA_A_a_sf
IPR013759 Topo_IIA_B_C
IPR013506 Topo_IIA_bsu_dom2
IPR031660 TOPRIM_C
IPR006171 TOPRIM_domain
PANTHERiPTHR10169 PTHR10169, 1 hit
PfamiView protein in Pfam
PF00204 DNA_gyraseB, 1 hit
PF00521 DNA_topoisoIV, 1 hit
PF16898 TOPRIM_C, 1 hit
SMARTiView protein in SMART
SM00433 TOP2c, 1 hit
SM00434 TOP4c, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55874 SSF55874, 1 hit
SSF56719 SSF56719, 1 hit
PROSITEiView protein in PROSITE
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QSK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSNHKKQY TTLSDIDHVL LRPEVIIGST VPMEQTEYVV DENFSEILEK
60 70 80 90 100
QVFVSEALIR IFVEVLANAV DNIHRSKGSS TPCKSIKIKI EDDGTTMIHN
110 120 130 140 150
DGQVIKISKE ENDGIEINGK KCSVYNHELV FGHLRSSSNY DDTVVRQTSG
160 170 180 190 200
KNGLGVKCTN ILSKSFKVIG VDPENKKKFV QEWTNNMKET SGPKVTTSSS
210 220 230 240 250
KTGYTEVIYC PDFERLGNFS SDIIGIFRKH VLDVAMLASS EGVSVYFNDK
260 270 280 290 300
KLPIKKFSDY TKLFKLEKES ISITEEDGSS EVTIAFVERD GKKKPISFVN
310 320 330 340 350
GLFTKHGGQH VDGWTKPFFA NLLSVLNKPS KTYKDLKLSL KDVTPYFRFF
360 370 380 390 400
VKSTVDKPQF DCQNKNMLKS PCLKYTIPDK IITKVCKWTS VQELKQSSFE
410 420 430 440 450
KLLKGKDNNI IKTLNDKKRR PKVSVKEYDP ANKAGTTLSE KCTLIVCEGL
460 470 480 490 500
SAKTYAVAGI STGISFTGTD TKKGRDWFGI LPLRGKFLNV RNANNDKMIK
510 520 530 540 550
NTVVTDLVNA MGLTFGMDYS TQKSRKSLNY GSIIIIPDPD EDGIHIEGLV
560 570 580 590 600
LNFFHHHFSS LFNPVVPNLK DFPFISSMKI PIIKVIEKPL KGRREIEFFT
610 620 630 640 650
HEAFHKFKNE NKWHKNHEIK YFKGLGTTKH EDVPKIFGKK MVVFNIDDNA
660 670 680 690 700
HENMEKAFKD EEADERKEWL KQYNPNARTF DLDSPNKILQ LNISTFINEE
710 720 730 740 750
LIKFSIEDCK RSLPHLIDGL KESQRKVIFG LKQWNGKNNI KVAQLGAFVA
760 770 780 790 800
QKTDYKHGEQ NLFDTIIKMA QTFVGANNIP LLEEDGQFGT RLSGGKDAAS
810 820 830 840 850
PRYIFVNQPP ILNKIFRPED DPILNYTLDG EPVFYAPVIP LICINGSVGV
860 870 880 890 900
GTGFSCNIPM FNPSEIIDGL KQWINMREQG EKYVFKYKPW YKGFTGKIEK
910 920 930 940 950
NGSGRYISYG TLSNEASNNG MYQYTVSELP INMWTDKFKE MCDQYRESNL
960 970 980 990 1000
IKTCDNYSEV TTVNFKISSE HELTIENLKL RSYIHTSNMV MFDHEGKIHK
1010 1020 1030 1040 1050
YETLNGIMQR FCKERIKIYE KRRQYMLQKL NKSLTISENK KRFMEDVMNQ
1060 1070 1080 1090 1100
KIKILLQEDE VVHQQLLDMN YYKDDEGEFD YLLNMNIGGF RKKNVDKLIS
1110 1120 1130
KIDDLKKDIM WYTNTNEGQM WLKDILELEP FI
Length:1,132
Mass (Da):129,877
Last modified:May 1, 2000 - v1
Checksum:i4626121A5FCC6201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF303741 Genomic DNA Translation: AAD48151.1
RefSeqiNP_149508.1, NC_003038.1

Genome annotation databases

GeneIDi921682
KEGGivg:921682

Similar proteinsi

Entry informationi

Entry nameiTOP2_IIV6
AccessioniPrimary (citable) accession number: Q9QSK1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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