Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9QSK1

- TOP2_IIV6

UniProt

Q9QSK1 - TOP2_IIV6

Protein

DNA topoisomerase 2

Gene

TOP2

Organism
Invertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Can introduce negative superhelical turns into double-stranded circular DNA.

    Catalytic activityi

    ATP-dependent breakage, passage and rejoining of double-stranded DNA.PROSITE-ProRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei68 – 681ATPBy similarity
    Binding sitei100 – 1001ATPBy similarity
    Metal bindingi448 – 4481Magnesium 1; catalyticPROSITE-ProRule annotation
    Metal bindingi538 – 5381Magnesium 1; catalyticPROSITE-ProRule annotation
    Metal bindingi538 – 5381Magnesium 2PROSITE-ProRule annotation
    Metal bindingi540 – 5401Magnesium 2PROSITE-ProRule annotation
    Sitei802 – 8021Transition state stabilizerBy similarity
    Active sitei803 – 8031O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi137 – 1393ATPBy similarity
    Nucleotide bindingi150 – 1578ATPBy similarity
    Nucleotide bindingi363 – 3653ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 2 (EC:5.99.1.3)
    Alternative name(s):
    DNA topoisomerase II
    Gene namesi
    Name:TOP2
    ORF Names:IIV6-045L
    OrganismiInvertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
    Taxonomic identifieri176652 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeIridovirus
    Virus hostiAcheta domesticus (House cricket) [TaxID: 6997]
    Chilo suppressalis (striped riceborer) [TaxID: 168631]
    Gryllus bimaculatus (Two-spotted cricket) [TaxID: 6999]
    Gryllus campestris [TaxID: 58607]
    Spodoptera frugiperda (Fall armyworm) [TaxID: 7108]
    ProteomesiUP000001359: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11321132DNA topoisomerase 2PRO_0000377771Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QSK1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini442 – 577136ToprimPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the type II topoisomerase family.Curated
    Contains 1 Toprim domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.10.268.10. 1 hit.
    3.30.1360.40. 1 hit.
    3.30.230.10. 1 hit.
    3.30.565.10. 1 hit.
    3.40.50.670. 1 hit.
    3.90.199.10. 1 hit.
    InterProiIPR024946. Arg_repress_C-like.
    IPR003594. HATPase_ATP-bd.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR001241. Topo_IIA.
    IPR002205. Topo_IIA_A/C.
    IPR013758. Topo_IIA_A/C_ab.
    IPR013757. Topo_IIA_A_a.
    IPR013506. Topo_IIA_bsu_dom2.
    IPR013759. Topo_IIA_cen_dom.
    IPR013760. Topo_IIA_like_dom.
    IPR006171. Toprim_domain.
    [Graphical view]
    PfamiPF00204. DNA_gyraseB. 1 hit.
    PF00521. DNA_topoisoIV. 1 hit.
    PF02518. HATPase_c. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00418. TPI2FAMILY.
    SMARTiSM00387. HATPase_c. 1 hit.
    SM00433. TOP2c. 1 hit.
    SM00434. TOP4c. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    SSF56719. SSF56719. 1 hit.
    PROSITEiPS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9QSK1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATSNHKKQY TTLSDIDHVL LRPEVIIGST VPMEQTEYVV DENFSEILEK     50
    QVFVSEALIR IFVEVLANAV DNIHRSKGSS TPCKSIKIKI EDDGTTMIHN 100
    DGQVIKISKE ENDGIEINGK KCSVYNHELV FGHLRSSSNY DDTVVRQTSG 150
    KNGLGVKCTN ILSKSFKVIG VDPENKKKFV QEWTNNMKET SGPKVTTSSS 200
    KTGYTEVIYC PDFERLGNFS SDIIGIFRKH VLDVAMLASS EGVSVYFNDK 250
    KLPIKKFSDY TKLFKLEKES ISITEEDGSS EVTIAFVERD GKKKPISFVN 300
    GLFTKHGGQH VDGWTKPFFA NLLSVLNKPS KTYKDLKLSL KDVTPYFRFF 350
    VKSTVDKPQF DCQNKNMLKS PCLKYTIPDK IITKVCKWTS VQELKQSSFE 400
    KLLKGKDNNI IKTLNDKKRR PKVSVKEYDP ANKAGTTLSE KCTLIVCEGL 450
    SAKTYAVAGI STGISFTGTD TKKGRDWFGI LPLRGKFLNV RNANNDKMIK 500
    NTVVTDLVNA MGLTFGMDYS TQKSRKSLNY GSIIIIPDPD EDGIHIEGLV 550
    LNFFHHHFSS LFNPVVPNLK DFPFISSMKI PIIKVIEKPL KGRREIEFFT 600
    HEAFHKFKNE NKWHKNHEIK YFKGLGTTKH EDVPKIFGKK MVVFNIDDNA 650
    HENMEKAFKD EEADERKEWL KQYNPNARTF DLDSPNKILQ LNISTFINEE 700
    LIKFSIEDCK RSLPHLIDGL KESQRKVIFG LKQWNGKNNI KVAQLGAFVA 750
    QKTDYKHGEQ NLFDTIIKMA QTFVGANNIP LLEEDGQFGT RLSGGKDAAS 800
    PRYIFVNQPP ILNKIFRPED DPILNYTLDG EPVFYAPVIP LICINGSVGV 850
    GTGFSCNIPM FNPSEIIDGL KQWINMREQG EKYVFKYKPW YKGFTGKIEK 900
    NGSGRYISYG TLSNEASNNG MYQYTVSELP INMWTDKFKE MCDQYRESNL 950
    IKTCDNYSEV TTVNFKISSE HELTIENLKL RSYIHTSNMV MFDHEGKIHK 1000
    YETLNGIMQR FCKERIKIYE KRRQYMLQKL NKSLTISENK KRFMEDVMNQ 1050
    KIKILLQEDE VVHQQLLDMN YYKDDEGEFD YLLNMNIGGF RKKNVDKLIS 1100
    KIDDLKKDIM WYTNTNEGQM WLKDILELEP FI 1132
    Length:1,132
    Mass (Da):129,877
    Last modified:May 1, 2000 - v1
    Checksum:i4626121A5FCC6201
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF303741 Genomic DNA. Translation: AAD48151.1.
    RefSeqiNP_149508.1. NC_003038.1.

    Genome annotation databases

    GeneIDi921682.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF303741 Genomic DNA. Translation: AAD48151.1 .
    RefSeqi NP_149508.1. NC_003038.1.

    3D structure databases

    ProteinModelPortali Q9QSK1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 921682.

    Family and domain databases

    Gene3Di 1.10.268.10. 1 hit.
    3.30.1360.40. 1 hit.
    3.30.230.10. 1 hit.
    3.30.565.10. 1 hit.
    3.40.50.670. 1 hit.
    3.90.199.10. 1 hit.
    InterProi IPR024946. Arg_repress_C-like.
    IPR003594. HATPase_ATP-bd.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR001241. Topo_IIA.
    IPR002205. Topo_IIA_A/C.
    IPR013758. Topo_IIA_A/C_ab.
    IPR013757. Topo_IIA_A_a.
    IPR013506. Topo_IIA_bsu_dom2.
    IPR013759. Topo_IIA_cen_dom.
    IPR013760. Topo_IIA_like_dom.
    IPR006171. Toprim_domain.
    [Graphical view ]
    Pfami PF00204. DNA_gyraseB. 1 hit.
    PF00521. DNA_topoisoIV. 1 hit.
    PF02518. HATPase_c. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00418. TPI2FAMILY.
    SMARTi SM00387. HATPase_c. 1 hit.
    SM00433. TOP2c. 1 hit.
    SM00434. TOP4c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    SSF56719. SSF56719. 1 hit.
    PROSITEi PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus."
      Jakob N.J., Mueller K., Bahr U., Darai G.
      Virology 286:182-196(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes."
      Eaton H.E., Metcalf J., Penny E., Tcherepanov V., Upton C., Brunetti C.R.
      Virol. J. 4:11-11(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.

    Entry informationi

    Entry nameiTOP2_IIV6
    AccessioniPrimary (citable) accession number: Q9QSK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3