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Q9QSK1

- TOP2_IIV6

UniProt

Q9QSK1 - TOP2_IIV6

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Protein
DNA topoisomerase 2
Gene
TOP2, IIV6-045L
Organism
Invertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Can introduce negative superhelical turns into double-stranded circular DNA.

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.

Cofactori

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681ATP By similarity
Binding sitei100 – 1001ATP By similarity
Metal bindingi448 – 4481Magnesium 1; catalytic By similarity
Metal bindingi538 – 5381Magnesium 1; catalytic By similarity
Metal bindingi538 – 5381Magnesium 2 By similarity
Metal bindingi540 – 5401Magnesium 2 By similarity
Sitei802 – 8021Transition state stabilizer By similarity
Active sitei803 – 8031O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi137 – 1393ATP By similarity
Nucleotide bindingi150 – 1578ATP By similarity
Nucleotide bindingi363 – 3653ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 2 (EC:5.99.1.3)
Alternative name(s):
DNA topoisomerase II
Gene namesi
Name:TOP2
ORF Names:IIV6-045L
OrganismiInvertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Taxonomic identifieri176652 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeIridovirus
Virus hostiAcheta domesticus (House cricket) [TaxID: 6997]
Chilo suppressalis (striped riceborer) [TaxID: 168631]
Gryllus bimaculatus (Two-spotted cricket) [TaxID: 6999]
Gryllus campestris [TaxID: 58607]
Spodoptera frugiperda (Fall armyworm) [TaxID: 7108]
ProteomesiUP000001359: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11321132DNA topoisomerase 2
PRO_0000377771Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9QSK1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini442 – 577136Toprim
Add
BLAST

Sequence similaritiesi

Contains 1 Toprim domain.

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEiPS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QSK1-1 [UniParc]FASTAAdd to Basket

« Hide

MATSNHKKQY TTLSDIDHVL LRPEVIIGST VPMEQTEYVV DENFSEILEK     50
QVFVSEALIR IFVEVLANAV DNIHRSKGSS TPCKSIKIKI EDDGTTMIHN 100
DGQVIKISKE ENDGIEINGK KCSVYNHELV FGHLRSSSNY DDTVVRQTSG 150
KNGLGVKCTN ILSKSFKVIG VDPENKKKFV QEWTNNMKET SGPKVTTSSS 200
KTGYTEVIYC PDFERLGNFS SDIIGIFRKH VLDVAMLASS EGVSVYFNDK 250
KLPIKKFSDY TKLFKLEKES ISITEEDGSS EVTIAFVERD GKKKPISFVN 300
GLFTKHGGQH VDGWTKPFFA NLLSVLNKPS KTYKDLKLSL KDVTPYFRFF 350
VKSTVDKPQF DCQNKNMLKS PCLKYTIPDK IITKVCKWTS VQELKQSSFE 400
KLLKGKDNNI IKTLNDKKRR PKVSVKEYDP ANKAGTTLSE KCTLIVCEGL 450
SAKTYAVAGI STGISFTGTD TKKGRDWFGI LPLRGKFLNV RNANNDKMIK 500
NTVVTDLVNA MGLTFGMDYS TQKSRKSLNY GSIIIIPDPD EDGIHIEGLV 550
LNFFHHHFSS LFNPVVPNLK DFPFISSMKI PIIKVIEKPL KGRREIEFFT 600
HEAFHKFKNE NKWHKNHEIK YFKGLGTTKH EDVPKIFGKK MVVFNIDDNA 650
HENMEKAFKD EEADERKEWL KQYNPNARTF DLDSPNKILQ LNISTFINEE 700
LIKFSIEDCK RSLPHLIDGL KESQRKVIFG LKQWNGKNNI KVAQLGAFVA 750
QKTDYKHGEQ NLFDTIIKMA QTFVGANNIP LLEEDGQFGT RLSGGKDAAS 800
PRYIFVNQPP ILNKIFRPED DPILNYTLDG EPVFYAPVIP LICINGSVGV 850
GTGFSCNIPM FNPSEIIDGL KQWINMREQG EKYVFKYKPW YKGFTGKIEK 900
NGSGRYISYG TLSNEASNNG MYQYTVSELP INMWTDKFKE MCDQYRESNL 950
IKTCDNYSEV TTVNFKISSE HELTIENLKL RSYIHTSNMV MFDHEGKIHK 1000
YETLNGIMQR FCKERIKIYE KRRQYMLQKL NKSLTISENK KRFMEDVMNQ 1050
KIKILLQEDE VVHQQLLDMN YYKDDEGEFD YLLNMNIGGF RKKNVDKLIS 1100
KIDDLKKDIM WYTNTNEGQM WLKDILELEP FI 1132
Length:1,132
Mass (Da):129,877
Last modified:May 1, 2000 - v1
Checksum:i4626121A5FCC6201
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF303741 Genomic DNA. Translation: AAD48151.1.
RefSeqiNP_149508.1. NC_003038.1.

Genome annotation databases

GeneIDi921682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF303741 Genomic DNA. Translation: AAD48151.1 .
RefSeqi NP_149508.1. NC_003038.1.

3D structure databases

ProteinModelPortali Q9QSK1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 921682.

Family and domain databases

Gene3Di 1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProi IPR024946. Arg_repress_C-like.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR006171. Toprim_domain.
[Graphical view ]
Pfami PF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00418. TPI2FAMILY.
SMARTi SM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEi PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus."
    Jakob N.J., Mueller K., Bahr U., Darai G.
    Virology 286:182-196(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes."
    Eaton H.E., Metcalf J., Penny E., Tcherepanov V., Upton C., Brunetti C.R.
    Virol. J. 4:11-11(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.

Entry informationi

Entry nameiTOP2_IIV6
AccessioniPrimary (citable) accession number: Q9QSK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3