Q9QSK1 (TOP2_IIV6) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 80.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: DNA topoisomerase 2 EC=5.99.1.3 Alternative name(s): DNA topoisomerase II | ||||
| Gene names |
| ||||
| Organism | Invertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus) [Reference proteome] | ||||
| Taxonomic identifier | 176652 [NCBI] | ||||
| Taxonomic lineage | Viruses › dsDNA viruses, no RNA stage › Iridoviridae › Iridovirus | ||||
| Virus host | Acheta domesticus (House cricket) [TaxID: 6997] Chilo suppressalis (striped riceborer) [TaxID: 168631] Gryllus bimaculatus (Two-spotted cricket) [TaxID: 6999] Gryllus campestris [TaxID: 58607] Spodoptera frugiperda (Fall armyworm) [TaxID: 7108] |
Protein attributes
| Sequence length | 1132 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Can introduce negative superhelical turns into double-stranded circular DNA. |
| Catalytic activity | ATP-dependent breakage, passage and rejoining of double-stranded DNA. |
| Cofactor | Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity. |
| Sequence similarities | Belongs to the type II topoisomerase family. Contains 1 Toprim domain. |
Ontologies
| Keywords | |
|---|---|
| Ligand | ATP-binding DNA-binding Magnesium Metal-binding Nucleotide-binding |
| Molecular function | Isomerase Topoisomerase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | DNA topological change Inferred from electronic annotation. Source: InterPro |
| Cellular_component | host chromosome Inferred from electronic annotation. Source: InterPro |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type II (ATP-hydrolyzing) activityInferred from electronic annotation. Source: EC metal ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1132 | 1132 | DNA topoisomerase 2 | PRO_0000377771 | |||||
Regions | |||||||||
| Domain | 442 – 577 | 136 | Toprim | ||||||
| Nucleotide binding | 137 – 139 | 3 | ATP By similarity | ||||||
| Nucleotide binding | 150 – 157 | 8 | ATP By similarity | ||||||
| Nucleotide binding | 363 – 365 | 3 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 803 | 1 | O-(5'-phospho-DNA)-tyrosine intermediate By similarity | ||||||
| Metal binding | 448 | 1 | Magnesium 1; catalytic By similarity | ||||||
| Metal binding | 538 | 1 | Magnesium 1; catalytic By similarity | ||||||
| Metal binding | 538 | 1 | Magnesium 2 By similarity | ||||||
| Metal binding | 540 | 1 | Magnesium 2 By similarity | ||||||
| Binding site | 68 | 1 | ATP By similarity | ||||||
| Binding site | 100 | 1 | ATP By similarity | ||||||
| Site | 802 | 1 | Transition state stabilizer By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus." Jakob N.J., Mueller K., Bahr U., Darai G. Virology 286:182-196(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [2] | "Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes." Eaton H.E., Metcalf J., Penny E., Tcherepanov V., Upton C., Brunetti C.R. Virol. J. 4:11-11(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF303741 Genomic DNA. Translation: AAD48151.1. |
| RefSeq | NP_149508.1. NC_003038.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1BJT based on UniProtKB P06786. |
| ProteinModelPortal | Q9QSK1. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 921682. |
Phylogenomic databases | |
| ProtClustDB | CLSP2510750. |
Family and domain databases | |
| Gene3D | 1.10.268.10. 1 hit. 3.30.1360.40. 1 hit. 3.30.230.10. 1 hit. 3.30.565.10. 1 hit. 3.40.50.670. 1 hit. 3.90.199.10. 1 hit. |
| InterPro | IPR024946. Arg_repress_C-like. IPR003594. HATPase_ATP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013506. Topo_IIA_bsu_dom2. IPR013759. Topo_IIA_cen_dom. IPR013760. Topo_IIA_like_dom. IPR006171. Toprim_domain. [Graphical view] |
| Pfam | PF00204. DNA_gyraseB. 1 hit. PF00521. DNA_topoisoIV. 1 hit. PF02518. HATPase_c. 1 hit. PF01751. Toprim. 1 hit. [Graphical view] |
| PRINTS | PR00418. TPI2FAMILY. |
| SMART | SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. SM00434. TOP4c. 1 hit. [Graphical view] |
| SUPFAM | SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit. |
| PROSITE | PS50880. TOPRIM. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | TOP2_IIV6 | ||||||||
| Accession | Primary (citable) accession number: Q9QSK1 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Viral Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |