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Q9QSK1

- TOP2_IIV6

UniProt

Q9QSK1 - TOP2_IIV6

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Protein

DNA topoisomerase 2

Gene

TOP2

Organism
Invertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Can introduce negative superhelical turns into double-stranded circular DNA.

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.PROSITE-ProRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681ATPBy similarity
Binding sitei100 – 1001ATPBy similarity
Metal bindingi448 – 4481Magnesium 1; catalyticPROSITE-ProRule annotation
Metal bindingi538 – 5381Magnesium 1; catalyticPROSITE-ProRule annotation
Metal bindingi538 – 5381Magnesium 2PROSITE-ProRule annotation
Metal bindingi540 – 5401Magnesium 2PROSITE-ProRule annotation
Sitei802 – 8021Transition state stabilizerBy similarity
Active sitei803 – 8031O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi137 – 1393ATPBy similarity
Nucleotide bindingi150 – 1578ATPBy similarity
Nucleotide bindingi363 – 3653ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 2 (EC:5.99.1.3)
Alternative name(s):
DNA topoisomerase II
Gene namesi
Name:TOP2
ORF Names:IIV6-045L
OrganismiInvertebrate iridescent virus 6 (IIV-6) (Chilo iridescent virus)
Taxonomic identifieri176652 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeIridovirus
Virus hostiAcheta domesticus (House cricket) [TaxID: 6997]
Chilo suppressalis (striped riceborer) [TaxID: 168631]
Gryllus bimaculatus (Two-spotted cricket) [TaxID: 6999]
Gryllus campestris [TaxID: 58607]
Spodoptera frugiperda (Fall armyworm) [TaxID: 7108]
ProteomesiUP000001359: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11321132DNA topoisomerase 2PRO_0000377771Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9QSK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini442 – 577136ToprimPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type II topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProiIPR024946. Arg_repress_C-like.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEiPS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QSK1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATSNHKKQY TTLSDIDHVL LRPEVIIGST VPMEQTEYVV DENFSEILEK
60 70 80 90 100
QVFVSEALIR IFVEVLANAV DNIHRSKGSS TPCKSIKIKI EDDGTTMIHN
110 120 130 140 150
DGQVIKISKE ENDGIEINGK KCSVYNHELV FGHLRSSSNY DDTVVRQTSG
160 170 180 190 200
KNGLGVKCTN ILSKSFKVIG VDPENKKKFV QEWTNNMKET SGPKVTTSSS
210 220 230 240 250
KTGYTEVIYC PDFERLGNFS SDIIGIFRKH VLDVAMLASS EGVSVYFNDK
260 270 280 290 300
KLPIKKFSDY TKLFKLEKES ISITEEDGSS EVTIAFVERD GKKKPISFVN
310 320 330 340 350
GLFTKHGGQH VDGWTKPFFA NLLSVLNKPS KTYKDLKLSL KDVTPYFRFF
360 370 380 390 400
VKSTVDKPQF DCQNKNMLKS PCLKYTIPDK IITKVCKWTS VQELKQSSFE
410 420 430 440 450
KLLKGKDNNI IKTLNDKKRR PKVSVKEYDP ANKAGTTLSE KCTLIVCEGL
460 470 480 490 500
SAKTYAVAGI STGISFTGTD TKKGRDWFGI LPLRGKFLNV RNANNDKMIK
510 520 530 540 550
NTVVTDLVNA MGLTFGMDYS TQKSRKSLNY GSIIIIPDPD EDGIHIEGLV
560 570 580 590 600
LNFFHHHFSS LFNPVVPNLK DFPFISSMKI PIIKVIEKPL KGRREIEFFT
610 620 630 640 650
HEAFHKFKNE NKWHKNHEIK YFKGLGTTKH EDVPKIFGKK MVVFNIDDNA
660 670 680 690 700
HENMEKAFKD EEADERKEWL KQYNPNARTF DLDSPNKILQ LNISTFINEE
710 720 730 740 750
LIKFSIEDCK RSLPHLIDGL KESQRKVIFG LKQWNGKNNI KVAQLGAFVA
760 770 780 790 800
QKTDYKHGEQ NLFDTIIKMA QTFVGANNIP LLEEDGQFGT RLSGGKDAAS
810 820 830 840 850
PRYIFVNQPP ILNKIFRPED DPILNYTLDG EPVFYAPVIP LICINGSVGV
860 870 880 890 900
GTGFSCNIPM FNPSEIIDGL KQWINMREQG EKYVFKYKPW YKGFTGKIEK
910 920 930 940 950
NGSGRYISYG TLSNEASNNG MYQYTVSELP INMWTDKFKE MCDQYRESNL
960 970 980 990 1000
IKTCDNYSEV TTVNFKISSE HELTIENLKL RSYIHTSNMV MFDHEGKIHK
1010 1020 1030 1040 1050
YETLNGIMQR FCKERIKIYE KRRQYMLQKL NKSLTISENK KRFMEDVMNQ
1060 1070 1080 1090 1100
KIKILLQEDE VVHQQLLDMN YYKDDEGEFD YLLNMNIGGF RKKNVDKLIS
1110 1120 1130
KIDDLKKDIM WYTNTNEGQM WLKDILELEP FI
Length:1,132
Mass (Da):129,877
Last modified:May 1, 2000 - v1
Checksum:i4626121A5FCC6201
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF303741 Genomic DNA. Translation: AAD48151.1.
RefSeqiNP_149508.1. NC_003038.1.

Genome annotation databases

GeneIDi921682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF303741 Genomic DNA. Translation: AAD48151.1 .
RefSeqi NP_149508.1. NC_003038.1.

3D structure databases

ProteinModelPortali Q9QSK1.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 921682.

Family and domain databases

Gene3Di 1.10.268.10. 1 hit.
3.30.1360.40. 1 hit.
3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
3.90.199.10. 1 hit.
InterProi IPR024946. Arg_repress_C-like.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013757. Topo_IIA_A_a.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR006171. Toprim_domain.
[Graphical view ]
Pfami PF00204. DNA_gyraseB. 1 hit.
PF00521. DNA_topoisoIV. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00418. TPI2FAMILY.
SMARTi SM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
SM00434. TOP4c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
PROSITEi PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the first complete DNA sequence of an invertebrate iridovirus: coding strategy of the genome of Chilo iridescent virus."
    Jakob N.J., Mueller K., Bahr U., Darai G.
    Virology 286:182-196(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Comparative genomic analysis of the family Iridoviridae: re-annotating and defining the core set of iridovirus genes."
    Eaton H.E., Metcalf J., Penny E., Tcherepanov V., Upton C., Brunetti C.R.
    Virol. J. 4:11-11(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.

Entry informationi

Entry nameiTOP2_IIV6
AccessioniPrimary (citable) accession number: Q9QSK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3