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Protein

Inner capsid protein VP2

Gene
N/A
Organism
Rotavirus A (strain Human/Japan/KU/1995 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand (By similarity).By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Inner capsid protein VP2
OrganismiRotavirus A (strain Human/Japan/KU/1995 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Taxonomic identifieri10952 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000001458: Genome

Subcellular locationi

Virion Curated
Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

GO - Cellular componenti

  1. viral inner capsid Source: UniProtKB-KW
  2. viral nucleocapsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Inner capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Inner capsid protein VP2PRO_0000368057Add
BLAST

Interactioni

Subunit structurei

Dimer (Potential). Interacts with VP1; this interaction presumably activates VP1. Interacts with VP3. Interacts with VP6. Interacts with NSP5 (By similarity).By similarityCurated

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 102102Interaction with VP1 and VP3By similarityAdd
BLAST
Regioni406 – 42621HydrophobicBy similarityAdd
BLAST
Regioni434 – 45421HydrophobicBy similarityAdd
BLAST
Regioni548 – 56922Leucine-zipper 1Sequence AnalysisAdd
BLAST
Regioni677 – 69822Leucine-zipper 2Sequence AnalysisAdd
BLAST

Domaini

The N-terminus is involved in VP1 binding.By similarity

Sequence similaritiesi

Belongs to the rotavirus VP2 family.Curated

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR007779. Rotavirus_VP2.
[Graphical view]
PfamiPF05087. Rota_VP2. 1 hit.
[Graphical view]
ProDomiPD008866. Rota_VP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Q9QNB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYRKRGAKR EDLSQQHERL QEKEIENNTD VTMENKNNNN NRKQRLSDKV
60 70 80 90 100
LSQKEEIITD VQDDIKIADE VKKSSKEESK QLLEILKTKE EHQKEVQYEI
110 120 130 140 150
LQKTIPTFEP KESILKKLED IRPEQAKKQM KLFRIFEPRQ LPIYRTNGEK
160 170 180 190 200
ELRNRWYWKL KKDTLPDGDY DVREYFLNLY DQILIEMPDY LLLKDMAVEN
210 220 230 240 250
KNSRDAGKVV DSETANICDA IFQDEETEGV IRRFIADMRQ QVQSDRNIVN
260 270 280 290 300
YPSILHPIDH AFNEYFLNHQ LVEPLNNEII FNYIPERIRN DVNYILNMDM
310 320 330 340 350
NLPSTARYIR PNLLQDRLNL HDNFESLWDT ITTSNYVLAR SVVPDLKEKE
360 370 380 390 400
LVSTEAQIQK MSQDLQLEAL TIQSETQFLA GINSQAANDC FKTLIAAMLS
410 420 430 440 450
QRTMSLDFVT TNYMSLISGM WLLTVIPNDM FLRESLVACE LAIINTIVYP
460 470 480 490 500
AFGMQRMHYR NGDPQTPFQI AEQQIQNFQV ANWLHFINNN RFRQVVIDGV
510 520 530 540 550
LNQTLNDNIR NGQVINQLME ALMQLSRQQF PTMPVDYKRS IQRGILLLSN
560 570 580 590 600
RLGQLVDLTR LVSYNYETLM ACITMNMQHV QTLTTEKLQL TSVTSLCMLI
610 620 630 640 650
GNTTVIPSPQ TLFHYYNVSV NFHSNYNERI NDAVAIITAA NRLNLYQKKM
660 670 680 690 700
KSIVEDFLKR LQIFDVPRVP DDQMYRLRDR LRLLPVERRR LDIFNLILMN
710 720 730 740 750
MEQIERASDK IAQGVLIAYR DMQLERDEMY GFVNIARNLD GYQQINLEEL
760 770 780 790 800
MRTGDYGQIT NMLLNNQPVA LVGALPFVTD SSVISLIAKL DATVFAQIVK
810 820 830 840 850
LRKVDTLKPI LYKINSDSND FYLVANYDWI PTSTTKVYKQ VPQPFDFRAS
860 870 880 890
MHMLTSNLTF TVYSDLLSFV SADTVEPINA IAFDNMRIMN EL
Length:892
Mass (Da):104,135
Last modified:May 1, 2000 - v1
Checksum:i717BB8DCC285375E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022766 mRNA. Translation: BAA84963.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022766 mRNA. Translation: BAA84963.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR007779. Rotavirus_VP2.
[Graphical view]
PfamiPF05087. Rota_VP2. 1 hit.
[Graphical view]
ProDomiPD008866. Rota_VP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

  1. Taniguchi K.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiVP2_ROTHK
AccessioniPrimary (citable) accession number: Q9QNB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: May 1, 2000
Last modified: January 7, 2015
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.