Reviewed, UniProtKB/Swiss-Prot Q9QJT4 (L_SHRV)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Snakehead rhabdovirus (SHRV)

Taxonomic identifier

103603 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Novirhabdovirus

Virus host

Gobiosoma bosc (naked goby) [TaxID: 203314]

Protein attributes

Sequence length	1983 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1983	1983	Large structural protein		PRO_0000297840
Regions
	Domain	559 – 756	198	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q9QJT4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 3158F671B780B50D	FASTA	1,983	225,025
	10 20 30 40 50 60 MEFFDLDLEV SQERLPAECS LNAPLNLSLS LQLFGRIEPK TENIRRQSRR ITKVLRERHN 70 80 90 100 110 120 GYRLQDLIID STRTQANLIP HLVSSASGDL NTPILEHWEM LSKYYQSLGY SLPSLDKFDF 130 140 150 160 170 180 KESAGYWNAA CSFRDMLLKS QKVEKKVNKQ QTYVIYDITF EFVEGVVFIH GGEDGFNDGF 190 200 210 220 230 240 LAGGAIAAMT YTELLALFKI LNQRAQALLM CNISKGLEPD MVPSPSTIHS IYAEADHMLR 250 260 270 280 290 300 MAGQGAIDLL KLWEPLVLTQ LGDVLGDRFG LEDDFKLTIR TEAALLADQL NLKRSFARMT 310 320 330 340 350 360 ELIKSETRKQ PLFQLFGLFK HFAYPRVYSR DTINTILGVS DKPSANDPEE YLHDQCEIRK 370 380 390 400 410 420 EFYTRYVKAY HRAPQLDLGG LSPGSYLRRA LEAGKMPNEK SPLYTNLEWF FVKFKKSIEW 430 440 450 460 470 480 PLSDTLSTFL SDKAITQNRS TWLDNETSSR DNSEKRLLLK FIKENEDSVA RVVAQAKEIY 490 500 510 520 530 540 DNEDDRIIAL KVKEMELKLK GRGFGLMTFK PRLLQVLRES IAKKTSKLFP EITMTASDLD 550 560 570 580 590 600 LKKRKFLVSR KSDDRRGYVH MSKSLDINKF CTSQRQFNSQ AVFQCLDELL GTGALFSRVH 610 620 630 640 650 660 EIFEKTWIVD GSASDPPNLK KFKDRYQKLK DLGIDAPHTW GDGVFSGLMG GIEGLCQYVW 670 680 690 700 710 720 TICLLLRVER VLSKTSLTHF VMAQGDNVII NLIIPIEIER DGSISPSEHR RVKSLSTSID 730 740 750 760 770 780 TQLAMELEKS GLTLKIEETL SSEHISIYGK DLHCPHHLTL SLKKAGSASI ISSEQYQDVP 790 800 810 820 830 840 TFLAGLSTSI ETISECVNDK VSAHLFGVIL AHAGWKSLCV SQTWKGWEYP YQKDETINRV 850 860 870 880 890 900 RSQGIKLTEG EQVTVEKRLE RNPDKRCLEW ILATSFLGSA LGMLPFPTPV DLEKRGVGDY 910 920 930 940 950 960 ITHRLALTKK ALSSRVLPRR IEKLIRSMVN LPHSRETDLA KLFDSPFSLN LATEEDATSV 970 980 990 1000 1010 1020 IKRLARSTLR DLDIKNERLR AHIDIMDRGL QDLDRELGDS ETINPRVAHL IRDITDEKES 1030 1040 1050 1060 1070 1080 EMFVTKFATA RTMRTVALEN PQDVSVVSLL NKKSRAKETY TIWRSKRAPA EDWECSTQRA 1090 1100 1110 1120 1130 1140 KIERDSSWGK NVIGVTSPSP VEAMSYRLVD PSTWEEEKKD QDFTINYYLS KPSLISHQAR 1150 1160 1170 1180 1190 1200 LERGPLVPYY GTQTQPLIAK AYNELKGNPK TNKALMLLSL RETIVKSGSN LDKLIMRLCE 1210 1220 1230 1240 1250 1260 RALDLDLNTL PSLRAQEEAS SGEGIRGGIK ESMSPVGPDN FYTNITHKVF NRRWLSGFHI 1270 1280 1290 1300 1310 1320 NIADFIIWGL TCTRKSITTQ GTLSGNLPIC IPACRGCLRR KEREFLDIDN PPRWECKEGV 1330 1340 1350 1360 1370 1380 KDKAYMYFTT WCDLPRLSTL PSLDPQDAVF MIGRQLACSK GQDSGAATKF YNVSPESLGL 1390 1400 1410 1420 1430 1440 LHPRMLLLGY SEGLIFSYLR SQHIVLGCLY HPTITELLPS LEKYTLETID QHARQLGYLF 1450 1460 1470 1480 1490 1500 QEEETAKELL NAGLCPYTPR AIPLTITELK NAVCITVSRS ISVTLETKKS IHLMPESGIS 1510 1520 1530 1540 1550 1560 EEEVVAGRHA ARTLGGLLNI KVPNLVYIDC DLTKGLLPWE PELPSEVLQS ENFKIDGKRV 1570 1580 1590 1600 1610 1620 TLYAKSQKRD NSIWEERGWT CSNSREILAK GVKTKSLFIH QSVPSHLDID PALIVVIGGG 1630 1640 1650 1660 1670 1680 LGGCVVPYLQ HWRRPPVIFT TLFSERERIS EDGDLIIPPE LLVRGLSGRM VERELLEAEL 1690 1700 1710 1720 1730 1740 CDVTVPGNRK AIVDAVKRRI KPNESVLLID EIENRGDAED VLQQSISTLL QSLEKHCSLT 1750 1760 1770 1780 1790 1800 SVHTIRESNV KHFTQRLNIL RRGRYEANLF WNRYNRRDQY EALIVIPSES RMTECTFSVA 1810 1820 1830 1840 1850 1860 SVQAAFQKID DGIEVEAKLE AHSWGLPELP PREKKILLGY VSSVFLKLGL VVIERHMSST 1870 1880 1890 1900 1910 1920 KLIDLLESAG PQMISWEEKQ THRSWASTDS IKEKGVTQDR IMALLCFAWT LKGLKHGVWD 1930 1940 1950 1960 1970 1980 TNMDAVVEKT VYITHGPRLC ALDEKPRVQY AEFKLQSKKR VEDLKGYLGA LLHLETFFPL GDR

References

[1]	"Production of recombinant snakehead rhabdovirus: the NV protein is not required for viral replication." Johnson M.C., Simon B.E., Kim C.H., Leong J.A. J. Virol. 74:2343-2350(2000) [PubMed: 10666265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF147498 Genomic RNA. Translation: AAD56771.1.
RefSeq	NP_050585.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1457774.
Enzyme and pathway databases
BRENDA	2.1.1.56. 308009. 2.7.7.48. 308009.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR001016. RNA_pol_L_viral. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_SHRV

Accession

Primary (citable) accession number: Q9QJT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	May 1, 2000
Last modified:	January 19, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)