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Protein

Large tegument protein deneddylase

Gene

U31

Organism
Human herpesvirus 6B (strain Z29) (HHV-6 variant B) (Human B lymphotropic virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Large tegument protein that plays multiple roles in the viral cycle. During viral entry, remains associated with the capsid while most of the tegument is detached and participates in the capsid transport toward the host nucleus. Plays a role in the routing of the capsid at the nuclear pore complex and subsequent uncoating. Within the host nucleus, acts as a deneddylase and promotes the degradation of nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These modifications prevent host cell cycle S-phase progression and create a favorable environment allowing efficient viral genome replication. Participates later in the secondary envelopment of capsids. Indeed, plays a linker role for the association of the outer viral tegument to the capsids.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 101Important for catalytic activityPROSITE-ProRule annotation
Active sitei23 – 231PROSITE-ProRule annotation
Active sitei156 – 1561PROSITE-ProRule annotation
Active sitei158 – 1581PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Large tegument protein deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Gene namesi
Name:U31
OrganismiHuman herpesvirus 6B (strain Z29) (HHV-6 variant B) (Human B lymphotropic virus)
Taxonomic identifieri36351 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeRoseolovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000006930 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20772077Large tegument protein deneddylasePRO_0000408401Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins. Interacts with U30. Interacts with capsid vertex specific component (CVSC) U50. Interacts with the major capsid protein U57.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9QJ37.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 221219Peptidase C76PROSITE-ProRule annotationAdd
BLAST
Repeati1096 – 111924LRR 1Add
BLAST
Repeati2045 – 206824LRR 2Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1185 – 121430Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi280 – 2834Poly-Lys

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 2 LRR (leucine-rich) repeats.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Leucine-rich repeat, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QJ37-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIITSSTNQ NDSKYGPRAG KQCMSNSFSF LHTVYLNGIN NSLNAGTIDA
60 70 80 90 100
IMEEGYHLDT ASTLALMLDN SDSQDYRLLT EIPRRIHSRY GVTQHELSRP
110 120 130 140 150
FNGTLDTQKI DNEVYFGLID FILYGKTKNC PAFAVITIGV LSRAIFFLNN
160 170 180 190 200
TLYLFDSHPT EREATAAIYI CQDIEEAYEL LTAHGTEGFY YDASFIFFIE
210 220 230 240 250
TSNLSLSSHD AELLILKTYK DPDIAITLDK FSSTEIHDIK KTDDIGSQQD
260 270 280 290 300
LVAAKTTYLE RAPQKRKKNS HSLELELNDK KKKDTASLTY YATEVDLIPS
310 320 330 340 350
FYELRSQFQS LFHDLKSFPI MKSQFNWTIY LQDSPMNPNQ PFATPFLWNR
360 370 380 390 400
VFHLLCQIVD VFVGVGSTND DSSKQKQQTI FINYLLPFKD FSEVFNEALT
410 420 430 440 450
ACQENNLDIL LIYNNYLCKT TTFRTLERIL LSKFLAIADN EHEKHYEWVK
460 470 480 490 500
SWTTQMLQEM PKKLDDIENY LKAYVSQNPV KHFHEFVCLN KAEKHNIAVL
510 520 530 540 550
LNEKRKEIQE DIERDKNIFA QLSNFIDKLG ETPALPIESE NVHKVHTSDI
560 570 580 590 600
TEAIVPRFMT ESIELPNIST LNNTQQLSLE KQISEKLTNT IHTLRNKFTK
610 620 630 640 650
IVQDNYNNLA AGFMPVTELN CLFAYLVNLY FNIEVLKHSG LNINTELLQE
660 670 680 690 700
VEKLYDNTQF LRFGTSHFNI NNLSNFTLSI RKMFVDFYNS QKPSDRASEI
710 720 730 740 750
LAAIESILAD PSKNKTIVNI EMIKSQLEEL GKMEISTTEN KQTAEITKQI
760 770 780 790 800
LGDQELTPIY DFLHHLSAYN LPNTTTVKNL HLHFILEKRP DIAAILHDKI
810 820 830 840 850
QSILDICIDD MLNDITVPEQ TFSTVLFLVD LFPNSTEKTA LFESVLTLRQ
860 870 880 890 900
LAKKCANLKT LEEFDDLAQF ITTNSEQLQN MMKQHFGKKI PTLMDHIKFL
910 920 930 940 950
YSQKIITAEE KNWIQRAKTA VITSPEELTA FLATAPTKHA LQTCKPELDK
960 970 980 990 1000
ALQRHMEEQM KQTAENDKKH ILTIRNTLEK RLNDILLILK DGQFSSLETV
1010 1020 1030 1040 1050
HLNLLETFLK QLQDNDLIIH FTHALLPVLK DIETTISKTI SDILEKILIK
1060 1070 1080 1090 1100
TPLNPEQMSK EEQKYTPLLS FLSKFKKTTF CTEDVKTEID QMQKSITFLT
1110 1120 1130 1140 1150
KIATSTNKYT RISHSVYGQE LNLYEERITE LKKETNKIKE QLSKEYAVAE
1160 1170 1180 1190 1200
KKILLSSQDA KTNKIYLVLN THTLKEIKNT QFRETAFAKA LTVEVNNKEN
1210 1220 1230 1240 1250
QLQELLNHFN AHLKAKMDQN HITKLSFDTK WTAFVSDSRL YFPDFVDIKL
1260 1270 1280 1290 1300
QDFISDPFKV ISQLMNKAAN EMPYIQAEIT LKWLTQLVHD INKFCLSAIS
1310 1320 1330 1340 1350
EFGKEAIPFN YAALRDLEYQ INTKYVEIEN KVICNETVEN TKNIPKLTKL
1360 1370 1380 1390 1400
LKELDPKRVA GGQKQYQTLM NKILTSETSM QQTYEKEQLK KEYFETVNNV
1410 1420 1430 1440 1450
ASFKLAFNFP KQRQNVERLM EKFKSLPKGQ PFEKFPEEND LFSDSLITEN
1460 1470 1480 1490 1500
YINGLRALLN FITAAQNYIQ NTLLKQWAVF QQQNFIPIDY SVANVKPISD
1510 1520 1530 1540 1550
LYARLRIERD RQVFYQVNSV FGTQLIVDET GVPLQFHNIF YNAVVKFFSL
1560 1570 1580 1590 1600
NYKQIHVPED TPRLVSSQYK LLSVCKSFII ILQQFWENII TLDLGPYLRD
1610 1620 1630 1640 1650
GTQNFKRELI PIVNLKLFIY IITQAWTASE DSTVSTAFEL PIKQFTLLIL
1660 1670 1680 1690 1700
CSHPEYLYGC LSHPTDLVIN SLAKSIDKDS LYDTFVVSHN PPEKPMHLMR
1710 1720 1730 1740 1750
SICIDTQLWQ SAKLMKDTFQ QTFFTQLCPQ NEKFFIYLTA FLILPYKFLN
1760 1770 1780 1790 1800
YIWIQYKPIT FTQRSYQNLI KDLCSEYVHQ NKITMSSVTP HEPDTIKSGE
1810 1820 1830 1840 1850
KITSKITVHK AQNTPTLTRL QAQEYVFDYI LYSFLTGYEM TFAMYIDTIE
1860 1870 1880 1890 1900
KTYLLCMRHL ENVLHDKDFQ SVLRARTFDI NYILKQSWTK NIVEHSIFSV
1910 1920 1930 1940 1950
QLNKIVSYLN HTNRATPNIP LILFNYDNEV VNVYLPPMST DPKKVAFYIK
1960 1970 1980 1990 2000
NPFHFPVQEY EATDLISFHL YPKTTDILNQ LPPNKTVSTR PYNLSSETLT
2010 2020 2030 2040 2050
TKNLSEPKFK QPTVTGLMPK SQSIILSTDT NVLETSPDIK ANTASAAIKD
2060 2070
VTLAREQISE FSESINTTLS KLKSLYL
Length:2,077
Mass (Da):240,285
Last modified:May 1, 2000 - v1
Checksum:i49D4C3F872A5BDF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157706 Genomic DNA. Translation: AAD49645.1.
RefSeqiNP_050212.1. NC_000898.1.

Genome annotation databases

GeneIDi1497033.
KEGGivg:1497033.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157706 Genomic DNA. Translation: AAD49645.1.
RefSeqiNP_050212.1. NC_000898.1.

3D structure databases

ProteinModelPortaliQ9QJ37.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1497033.
KEGGivg:1497033.

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Human herpesvirus 6B genome sequence: coding content and comparison with human herpesvirus 6A."
    Dominguez G., Dambaugh T.R., Stamey F.R., Dewhurst S., Inoue N., Pellett P.E.
    J. Virol. 73:8040-8052(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiLTP_HHV6Z
AccessioniPrimary (citable) accession number: Q9QJ37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: May 1, 2000
Last modified: April 29, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.