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Q9QEJ5

- POLG_PESV

UniProt

Q9QEJ5 - POLG_PESV

Protein

Genome polyprotein

Gene

ORF1

Organism
Porcine enteric sapovirus (isolate Swine/United States/Cowden/1980) (Sw/SV/Cowden/1980/US)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity By similarity.By similarity
    Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.By similarity
    Protease-polymerase p70 processes the polyprotein: Pro-Pol is first released by autocleavage, then all other proteins are cleaved By similarity. It is also an RNA-directed RNA polymerase which replicates genomic and antigenomic viral RNA by recognizing specific signals. Catalyzes the covalent attachment VPg with viral RNAs By similarity.By similarity
    Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm By similarity.By similarity

    Catalytic activityi

    NTP + H2O = NDP + phosphate.
    Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei56 – 572Cleavage; by Pro-PolBy similarity
    Sitei310 – 3112Cleavage; by Pro-PolBy similarity
    Sitei649 – 6502Cleavage; by Pro-PolBy similarity
    Sitei934 – 9352Cleavage; by Pro-PolBy similarity
    Sitei1047 – 10482Cleavage; by Pro-PolBy similarity
    Active sitei1078 – 10781For protease activityBy similarity
    Active sitei1099 – 10991For protease activityBy similarity
    Active sitei1163 – 11631For protease activityBy similarity
    Sitei1712 – 17132Cleavage; by Pro-PolBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi464 – 4718ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. RNA binding Source: InterPro
    4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    5. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. RNA-protein covalent cross-linking Source: UniProtKB-KW
    3. transcription, DNA-templated Source: InterPro
    4. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    DNA replication, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Protein family/group databases

    MEROPSiC24.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Genome polyprotein
    Cleaved into the following 7 chains:
    Alternative name(s):
    p35
    Alternative name(s):
    VPg
    p14
    Protease-polymerase p70 (EC:2.7.7.48, EC:3.4.22.66)
    Short name:
    Pro-Pol
    Capsid protein
    Short name:
    CP
    Alternative name(s):
    VP1
    p60
    Gene namesi
    ORF Names:ORF1
    OrganismiPorcine enteric sapovirus (isolate Swine/United States/Cowden/1980) (Sw/SV/Cowden/1980/US)
    Taxonomic identifieri523795 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeSapovirus
    Virus hostiSus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cytoplasm, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 22542254Genome polyproteinPRO_0000341637Add
    BLAST
    Chaini1 – 5656Protein p11PRO_0000341638Add
    BLAST
    Chaini57 – 310254Protein p28PRO_0000341639Add
    BLAST
    Chaini311 – 650340NTPasePRO_0000341640Add
    BLAST
    Chaini651 – 934284Protein p32PRO_0000341641Add
    BLAST
    Chaini935 – 1048114Viral genome-linked proteinPRO_0000341642Add
    BLAST
    Chaini1049 – 1712664Protease-polymerase p70PRO_0000341643Add
    BLAST
    Chaini1713 – 2254542Capsid proteinPRO_0000341644Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei956 – 9561O-(5'-phospho-RNA)-tyrosineBy similarity

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins. Pro-Pol is first autocatalytically cleaved, then processes the whole polyprotein.
    VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.By similarity

    Keywords - PTMi

    Covalent protein-RNA linkage, Phosphoprotein

    Interactioni

    Subunit structurei

    Capsid protein homodimerizes, then multimerizes.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QEJ5.
    SMRiQ9QEJ5. Positions 1202-1693.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini438 – 592155SF3 helicasePROSITE-ProRule annotationAdd
    BLAST
    Domaini1063 – 1169107Peptidase C24Add
    BLAST
    Domaini1434 – 1559126RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    Protease-polymerase is composed of two domains displaying two different catalytic activity. These activities may act independently.

    Sequence similaritiesi

    Contains 1 peptidase C24 domain.Curated
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
    Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.60.120.20. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR004005. Calicivirus_coat.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR000317. Peptidase_C24.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF00915. Calici_coat. 1 hit.
    PF03510. Peptidase_C24. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    PRINTSiPR00916. 2CENDOPTASE.
    PR00918. CALICVIRUSNS.
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative promoter usage. Align

    Isoform Genome polyprotein (identifier: Q9QEJ5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MANCRPLPIG QLPNRIFDTP RLTPGWVWAC TSEATFKLEW LQDPVVIRPP     50
    DVFVAQGVVD DFFRPKRVLQ GDPQLIAQVL LGDANGPLVG PVSMQQLTSL 100
    LHEVSQALSD HKHPLANRYT RASLQRYADT LSNYIPLVDI LTGPKDLTPR 150
    DVLEQLAAGR EWECVPDSAL KKVFRDMWQY ICEGCDSVYI KLQDVKRKMP 200
    HIDTTVLKQF IITLTDTISM ATALDTKTWL AHILGWLKPT CLVMIMQQHV 250
    NSPQGWAATL TALAELYYGI MPLTETLGSV ASWVTDKFAD MATSTWGKFK 300
    SWWDSLYTPQ AGNDLIILGG VVGLVYFMVF GDAPTQMFTK KLMRVCGFIT 350
    STVAAIKAAM WIVDYFKQRE HEHQVRITLA RWAALQEVIK QNRCAGLSEV 400
    TKLKECCEVL LNEVTELMYK LGASPLAGLI RSTSDVIQTT INELAQLMAY 450
    DTQRKPPAMV VFGGPPGIGK TRLVEALAKQ LGEVSHFTMT VDHYDTYTGN 500
    TVAIWDEFDV DSKQAFIEAT IGIVNCAPYP LNCDRPEAKG RVFTSKYVLA 550
    TTNCPTPVMP DHPRAMAFWR RITFIDVTAP TIEQWLVDNP GRKAPTSLFK 600
    DDFSHLQCSV RGYTAYDEKG NTLSGKVARA RYVSVNNLLD LIKEKYNSEA 650
    ADVKHLWFTV PQAIHKQARD IILGWLRFHS YPNTVADNIP LSEVRDPTCF 700
    GYVVISDVDP PRHVAEHVAH IEVESILRTD IVGLLREGGG GLFRALKVKS 750
    APRNCIINKV MMQAHHTTLQ VLTSQEPHPP NLPRPRRLVF VESPIDIISA 800
    LRHHVGFCTI PGIVKLITSG VGLGVENLGN FLQSIAGNVR FPLQSECSLL 850
    RTPSGDVLFY TSGQAAVWAT PARFPIVTPG EASVGKEVCS ESSWWDILKA 900
    LFSTLVVAFG PIATLVLTAH NLAYLNTREN TLSEAKGKNK RGRGARRAIA 950
    LRDDEYDEWQ DIIRDWRKEM TVQQFLDLKE RALSGASDPD SQRYNAWLEL 1000
    RAKRLSAGAY QHAVVDIIGK SGHRREVIRT QVMRAPREPK GDTYDSEGRG 1050
    YVVPMTAQEK HTGWAVHIGN GRLVTCTHVA NMCDRVAEVE FKVAETDRDT 1100
    CIITAPLGHL PSVALGNGPP AFYTTNFHPI RVLDEGSWDT TTTRVTGWRV 1150
    VINNGTTTAP GDCGQPYLNA RRQLVGVHAA TSTCGVKKLV SRVQTKKTAK 1200
    ATFPWKGLPV TTMPDAGGLP TGTRYHRSIA WPKLLPEETH APAPYGVNDP 1250
    RHPFSQHQMI ANNLQPYINT PVALDQTLLQ RAVKHTKGYL DQIIGTHRSP 1300
    NLTYAAAVES MAHDTACGPN LPGRKKDYMT DQGEPIGPLK QMLEEAWDMA 1350
    HRGVPRRHEY KLALKDELRP IEKNDQGKKR LLWGCDAGVS MIANAVFKPV 1400
    TERLVDTVPM HPVAVGICMD SPQIEQMNQA LTGRVLYCLD YSKWDSTQNP 1450
    AVTCASVDIL ASYAEDTPLS SAAIATLCSP AVGRLDDIGL TVTSGLPSGM 1500
    PFTSVINSVN HMIYFAMAVL EAYEEFKVPY MGNIFDNETI YTYGDDCVYG 1550
    LTPATASIMP VVVKNLTSYG LVPTAADKSQ SIEPTDTPVF LKRTFSQTPF 1600
    GLRALLDETS LARQCYWVKA NRTTDLFEPA AVDVDIRKNQ LEVMLAYASQ 1650
    HPRSVFDKLA GMAEVTASAE GYQLVNVNWA NAVATYNAWY GGTDGGRAPT 1700
    NEDEEPEVFV MEAPAPTRSV ASNPEGTQNS NESRPVQPAG PMPVAAAQAL 1750
    EMAVATGQIN DTIPSVVRET FSTYTNVTWT TRQPTGTLLA RMSLGPGLNP 1800
    YTLHLSAMWA GWGGSFEIKV IISGSGMYAG KLLCALIPPG VDPSAVDQPG 1850
    AFPHALVDAR ITDGVTFTLG DVRAVDYHET GVGGAIASLA LYVYQPLINP 1900
    FETAVSAAMV TIETRPGPDF GFTLLKPPNQ AMEVGFDPRS LLPRTARTLR 1950
    GNRFGRPITA VVIVGVAQQI NRHFSAEGTT LGWSTAPIGP CVARVNGKHT 2000
    DNTGRAVFQL GPLSNGPLYP NIINHYPDVA ASTIFNTGTA VNDNTTGGGG 2050
    PMVIFNDVGD VVEDVAYQMR FIASHATSQS PTLIDQINAT SMAVCSFGNS 2100
    RADLNQNQLN VGIELTYTCG NTAINGIVTS FMDRQYTFGP QGPNNIMLWV 2150
    ESVLGTHTGN NTVYSSQPDT VSAALQGQPF NIPDGYMAVW NVNADSADFQ 2200
    IGLRRDGYFV TNGAIGTRMV ISEDTTFSFN GMYTLTTPLI GPSGTSGRSI 2250
    HSSR 2254

    Note: Produced from the genomic RNA.

    Length:2,254
    Mass (Da):247,170
    Last modified:May 1, 2000 - v1
    Checksum:i90CA9F0314FAF739
    GO
    Isoform Subgenomic capsid protein (identifier: Q9QEJ5-2) [UniParc]FASTAAdd to Basket

    Also known as: VP1

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1710: Missing.

    Note: Produced from the subgenomic RNA.

    Show »
    Length:544
    Mass (Da):57,615
    Checksum:i15FA9B93FA05D946
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 17101710Missing in isoform Subgenomic capsid protein. CuratedVSP_034378Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF182760 Genomic RNA. Translation: AAF04560.1.
    RefSeqiNP_051035.1. NC_000940.1.

    Genome annotation databases

    GeneIDi1457802.

    Keywords - Coding sequence diversityi

    Alternative promoter usage

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF182760 Genomic RNA. Translation: AAF04560.1 .
    RefSeqi NP_051035.1. NC_000940.1.

    3D structure databases

    ProteinModelPortali Q9QEJ5.
    SMRi Q9QEJ5. Positions 1202-1693.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C24.003.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1457802.

    Family and domain databases

    Gene3Di 2.60.120.20. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR004005. Calicivirus_coat.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR000317. Peptidase_C24.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF00915. Calici_coat. 1 hit.
    PF03510. Peptidase_C24. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    PRINTSi PR00916. 2CENDOPTASE.
    PR00918. CALICVIRUSNS.
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of a porcine enteric calicivirus genetically related to Sapporo-like human caliciviruses."
      Guo M., Chang K.O., Hardy M.E., Zhang Q., Parwani A.V., Saif L.J.
      J. Virol. 73:9625-9631(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Expression and self-assembly in baculovirus of porcine enteric calicivirus capsids into virus-like particles and their use in an enzyme-linked immunosorbent assay for antibody detection in swine."
      Guo M., Qian Y., Chang K.O., Saif L.J.
      J. Clin. Microbiol. 39:1487-1493(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION OF CAPSID PROTEIN.

    Entry informationi

    Entry nameiPOLG_PESV
    AccessioniPrimary (citable) accession number: Q9QEJ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Two differents RNAs lead the expression of the capsid protein. One arises from the cleavage of the polyprotein translated from the genomic RNA and the other from the translation of a subgenomic RNA derived from the (-)RNA template. Capsid protein expressed from the subgenomic mRNA is produced in much larger amounts than the cleaved one By similarity.By similarity

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3