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Q9QEJ5

- POLG_PESV

UniProt

Q9QEJ5 - POLG_PESV

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Protein
Genome polyprotein
Gene
ORF1
Organism
Porcine enteric sapovirus (isolate Swine/United States/Cowden/1980) (Sw/SV/Cowden/1980/US)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity By similarity.
Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.
Protease-polymerase p70 processes the polyprotein: Pro-Pol is first released by autocleavage, then all other proteins are cleaved By similarity. It is also an RNA-directed RNA polymerase which replicates genomic and antigenomic viral RNA by recognizing specific signals. Catalyzes the covalent attachment VPg with viral RNAs By similarity.
Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells, inducing endocytosis of the viral particle. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm By similarity.

Catalytic activityi

NTP + H2O = NDP + phosphate.
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei56 – 572Cleavage; by Pro-Pol By similarity
Sitei310 – 3112Cleavage; by Pro-Pol By similarity
Sitei649 – 6502Cleavage; by Pro-Pol By similarity
Sitei934 – 9352Cleavage; by Pro-Pol By similarity
Sitei1047 – 10482Cleavage; by Pro-Pol By similarity
Active sitei1078 – 10781For protease activity By similarity
Active sitei1099 – 10991For protease activity By similarity
Active sitei1163 – 11631For protease activity By similarity
Sitei1712 – 17132Cleavage; by Pro-Pol By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi464 – 4718ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. RNA helicase activity Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. cysteine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. RNA-protein covalent cross-linking Source: UniProtKB-KW
  3. transcription, DNA-templated Source: InterPro
  4. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

DNA replication, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiC24.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 7 chains:
Alternative name(s):
p35
Alternative name(s):
VPg
p14
Protease-polymerase p70 (EC:2.7.7.48, EC:3.4.22.66)
Short name:
Pro-Pol
Capsid protein
Short name:
CP
Alternative name(s):
VP1
p60
Gene namesi
ORF Names:ORF1
OrganismiPorcine enteric sapovirus (isolate Swine/United States/Cowden/1980) (Sw/SV/Cowden/1980/US)
Taxonomic identifieri523795 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridaeSapovirus
Virus hostiSus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22542254Genome polyprotein
PRO_0000341637Add
BLAST
Chaini1 – 5656Protein p11
PRO_0000341638Add
BLAST
Chaini57 – 310254Protein p28
PRO_0000341639Add
BLAST
Chaini311 – 650340NTPase
PRO_0000341640Add
BLAST
Chaini651 – 934284Protein p32
PRO_0000341641Add
BLAST
Chaini935 – 1048114Viral genome-linked protein
PRO_0000341642Add
BLAST
Chaini1049 – 1712664Protease-polymerase p70
PRO_0000341643Add
BLAST
Chaini1713 – 2254542Capsid protein
PRO_0000341644Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei956 – 9561O-(5'-phospho-RNA)-tyrosine By similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. Pro-Pol is first autocatalytically cleaved, then processes the whole polyprotein.
VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Interactioni

Subunit structurei

Capsid protein homodimerizes, then multimerizes By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ9QEJ5.
SMRiQ9QEJ5. Positions 1202-1693.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini438 – 592155SF3 helicase
Add
BLAST
Domaini1063 – 1169107Peptidase C24
Add
BLAST
Domaini1434 – 1559126RdRp catalytic
Add
BLAST

Domaini

Protease-polymerase is composed of two domains displaying two different catalytic activity. These activities may act independently.

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.20. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR004005. Calicivirus_coat.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view]
PfamiPF00915. Calici_coat. 1 hit.
PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. Align

Isoform Genome polyprotein (identifier: Q9QEJ5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MANCRPLPIG QLPNRIFDTP RLTPGWVWAC TSEATFKLEW LQDPVVIRPP     50
DVFVAQGVVD DFFRPKRVLQ GDPQLIAQVL LGDANGPLVG PVSMQQLTSL 100
LHEVSQALSD HKHPLANRYT RASLQRYADT LSNYIPLVDI LTGPKDLTPR 150
DVLEQLAAGR EWECVPDSAL KKVFRDMWQY ICEGCDSVYI KLQDVKRKMP 200
HIDTTVLKQF IITLTDTISM ATALDTKTWL AHILGWLKPT CLVMIMQQHV 250
NSPQGWAATL TALAELYYGI MPLTETLGSV ASWVTDKFAD MATSTWGKFK 300
SWWDSLYTPQ AGNDLIILGG VVGLVYFMVF GDAPTQMFTK KLMRVCGFIT 350
STVAAIKAAM WIVDYFKQRE HEHQVRITLA RWAALQEVIK QNRCAGLSEV 400
TKLKECCEVL LNEVTELMYK LGASPLAGLI RSTSDVIQTT INELAQLMAY 450
DTQRKPPAMV VFGGPPGIGK TRLVEALAKQ LGEVSHFTMT VDHYDTYTGN 500
TVAIWDEFDV DSKQAFIEAT IGIVNCAPYP LNCDRPEAKG RVFTSKYVLA 550
TTNCPTPVMP DHPRAMAFWR RITFIDVTAP TIEQWLVDNP GRKAPTSLFK 600
DDFSHLQCSV RGYTAYDEKG NTLSGKVARA RYVSVNNLLD LIKEKYNSEA 650
ADVKHLWFTV PQAIHKQARD IILGWLRFHS YPNTVADNIP LSEVRDPTCF 700
GYVVISDVDP PRHVAEHVAH IEVESILRTD IVGLLREGGG GLFRALKVKS 750
APRNCIINKV MMQAHHTTLQ VLTSQEPHPP NLPRPRRLVF VESPIDIISA 800
LRHHVGFCTI PGIVKLITSG VGLGVENLGN FLQSIAGNVR FPLQSECSLL 850
RTPSGDVLFY TSGQAAVWAT PARFPIVTPG EASVGKEVCS ESSWWDILKA 900
LFSTLVVAFG PIATLVLTAH NLAYLNTREN TLSEAKGKNK RGRGARRAIA 950
LRDDEYDEWQ DIIRDWRKEM TVQQFLDLKE RALSGASDPD SQRYNAWLEL 1000
RAKRLSAGAY QHAVVDIIGK SGHRREVIRT QVMRAPREPK GDTYDSEGRG 1050
YVVPMTAQEK HTGWAVHIGN GRLVTCTHVA NMCDRVAEVE FKVAETDRDT 1100
CIITAPLGHL PSVALGNGPP AFYTTNFHPI RVLDEGSWDT TTTRVTGWRV 1150
VINNGTTTAP GDCGQPYLNA RRQLVGVHAA TSTCGVKKLV SRVQTKKTAK 1200
ATFPWKGLPV TTMPDAGGLP TGTRYHRSIA WPKLLPEETH APAPYGVNDP 1250
RHPFSQHQMI ANNLQPYINT PVALDQTLLQ RAVKHTKGYL DQIIGTHRSP 1300
NLTYAAAVES MAHDTACGPN LPGRKKDYMT DQGEPIGPLK QMLEEAWDMA 1350
HRGVPRRHEY KLALKDELRP IEKNDQGKKR LLWGCDAGVS MIANAVFKPV 1400
TERLVDTVPM HPVAVGICMD SPQIEQMNQA LTGRVLYCLD YSKWDSTQNP 1450
AVTCASVDIL ASYAEDTPLS SAAIATLCSP AVGRLDDIGL TVTSGLPSGM 1500
PFTSVINSVN HMIYFAMAVL EAYEEFKVPY MGNIFDNETI YTYGDDCVYG 1550
LTPATASIMP VVVKNLTSYG LVPTAADKSQ SIEPTDTPVF LKRTFSQTPF 1600
GLRALLDETS LARQCYWVKA NRTTDLFEPA AVDVDIRKNQ LEVMLAYASQ 1650
HPRSVFDKLA GMAEVTASAE GYQLVNVNWA NAVATYNAWY GGTDGGRAPT 1700
NEDEEPEVFV MEAPAPTRSV ASNPEGTQNS NESRPVQPAG PMPVAAAQAL 1750
EMAVATGQIN DTIPSVVRET FSTYTNVTWT TRQPTGTLLA RMSLGPGLNP 1800
YTLHLSAMWA GWGGSFEIKV IISGSGMYAG KLLCALIPPG VDPSAVDQPG 1850
AFPHALVDAR ITDGVTFTLG DVRAVDYHET GVGGAIASLA LYVYQPLINP 1900
FETAVSAAMV TIETRPGPDF GFTLLKPPNQ AMEVGFDPRS LLPRTARTLR 1950
GNRFGRPITA VVIVGVAQQI NRHFSAEGTT LGWSTAPIGP CVARVNGKHT 2000
DNTGRAVFQL GPLSNGPLYP NIINHYPDVA ASTIFNTGTA VNDNTTGGGG 2050
PMVIFNDVGD VVEDVAYQMR FIASHATSQS PTLIDQINAT SMAVCSFGNS 2100
RADLNQNQLN VGIELTYTCG NTAINGIVTS FMDRQYTFGP QGPNNIMLWV 2150
ESVLGTHTGN NTVYSSQPDT VSAALQGQPF NIPDGYMAVW NVNADSADFQ 2200
IGLRRDGYFV TNGAIGTRMV ISEDTTFSFN GMYTLTTPLI GPSGTSGRSI 2250
HSSR 2254

Note: Produced from the genomic RNA.

Length:2,254
Mass (Da):247,170
Last modified:May 1, 2000 - v1
Checksum:i90CA9F0314FAF739
GO
Isoform Subgenomic capsid protein (identifier: Q9QEJ5-2) [UniParc]FASTAAdd to Basket

Also known as: VP1

The sequence of this isoform differs from the canonical sequence as follows:
     1-1710: Missing.

Note: Produced from the subgenomic RNA.

Show »
Length:544
Mass (Da):57,615
Checksum:i15FA9B93FA05D946
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 17101710Missing in isoform Subgenomic capsid protein.
VSP_034378Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF182760 Genomic RNA. Translation: AAF04560.1.
RefSeqiNP_051035.1. NC_000940.1.

Genome annotation databases

GeneIDi1457802.

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF182760 Genomic RNA. Translation: AAF04560.1 .
RefSeqi NP_051035.1. NC_000940.1.

3D structure databases

ProteinModelPortali Q9QEJ5.
SMRi Q9QEJ5. Positions 1202-1693.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C24.003.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1457802.

Family and domain databases

Gene3Di 2.60.120.20. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR004005. Calicivirus_coat.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
[Graphical view ]
Pfami PF00915. Calici_coat. 1 hit.
PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of a porcine enteric calicivirus genetically related to Sapporo-like human caliciviruses."
    Guo M., Chang K.O., Hardy M.E., Zhang Q., Parwani A.V., Saif L.J.
    J. Virol. 73:9625-9631(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Expression and self-assembly in baculovirus of porcine enteric calicivirus capsids into virus-like particles and their use in an enzyme-linked immunosorbent assay for antibody detection in swine."
    Guo M., Qian Y., Chang K.O., Saif L.J.
    J. Clin. Microbiol. 39:1487-1493(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF CAPSID PROTEIN.

Entry informationi

Entry nameiPOLG_PESV
AccessioniPrimary (citable) accession number: Q9QEJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Two differents RNAs lead the expression of the capsid protein. One arises from the cleavage of the polyprotein translated from the genomic RNA and the other from the translation of a subgenomic RNA derived from the (-)RNA template. Capsid protein expressed from the subgenomic mRNA is produced in much larger amounts than the cleaved one By similarity.

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi