Reviewed, UniProtKB/Swiss-Prot Q9QC07 (POK7_HUMAN)

Names and origin

Protein names	Recommended name:     HERV-K_1q23.3 provirus ancestral Pol protein Alternative name(s):     HERV-K18 Pol protein     HERV-K110 Pol protein     HERV-K(C1a) Pol protein Including the following 2 domains:     1- Recommended name:             Reverse transcriptase               EC=2.7.7.49     2- Recommended name:             Ribonuclease H                 Short name=RNase H               EC=3.1.26.4
Organism	Homo sapiens (Human) [Complete proteome]
Taxonomic identifier	9606 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	812 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Early post-infection, the reverse transcriptase converts the viral RNA genome into double-stranded viral DNA. The RNase H domain of the reverse transcriptase performs two functions. It degrades the RNA template and specifically removes the RNA primer from the RNA/DNA hybrid. Following nuclear import, the integrase catalyzes the insertion of the linear, double-stranded viral DNA into the host cell chromosome. Endogenous Pol proteins may have kept, lost or modified their original function during evolution.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Endonucleolytic cleavage to 5'-phosphomonoester.
Domain	The LPQG and YXDD motifs are catalytically important and conserved among many retroviruses.
Miscellaneous	This protein is synthesized as Gag-Pro and Gag-Pro-Pol polyprotein precursors. These polyproteins are thought, by similarity with type-B retroviruses, to be generated by -1 frameshifts occurring at the Gag-Pro and Pro-Pol genes boundaries. Exact N-terminus of this protein has not been formally described. Intragenic, in the first intron of CD48 gene.
Sequence similarities	Belongs to the beta type-B retroviral polymerase family. HERV class-II K(HML-2) subfamily. Contains 1 integrase catalytic domain. Contains 1 integrase-type zinc finger. Contains 1 reverse transcriptase domain. Contains 1 RNase H domain.
Caution	Truncated; premature stop codon after the RNase H domain.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Endonuclease Hydrolase Nuclease Nucleotidyltransferase RNA-directed DNA polymerase Transferase
Technical term	Complete proteome ERV Multifunctional enzyme Transposable element
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: InterPro RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW integrase activity Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 812	812	HERV-K_1q23.3 provirus ancestral Pol protein		PRO_0000186767
Regions
	Domain	57 – 245	189	Reverse transcriptase
	Domain	461 – 590	130	RNase H
	Domain	637 – 803	167	Integrase catalytic
	Zinc finger	587 – 628	42	Integrase-type
	Motif	161 – 164	4	LPQG
	Motif	195 – 198	4	YXDD
Experimental info
	Sequence conflict	171	1	I → L in CAB56603. Ref.1
	Sequence conflict	457	1	R → C in AL121985. Ref.3
	Sequence conflict	614 – 812	199	Missing Ref.3
	Sequence conflict	631	1	D → N in AAL60056. Ref.2
	Sequence conflict	643 – 662	20	VCVLM…LHLGR → GLCPNALWQMDVTHVPSFGK in AAL60056. Ref.2
	Sequence conflict	678	1	C → W in AAL60056. Ref.2
	Sequence conflict	705	1	E → K in AAL60056. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9QC07-1 [UniParc]. Last modified September 13, 2004. Version 2. Checksum: 6E25F504DD1F0183	FASTA	812	91,948
	10 20 30 40 50 60 NKSRKRRNRV SFLGVTTVEP PKPIPLTWKT EKLVWVNQWP LPKQKLEALH LLANEQLEKG 70 80 90 100 110 120 HIEPSFSPWN SPVFVIQKKS SKWRMLTDLR AVNAVIQPMG PLQPGLPSPA MIPKDWPLII 130 140 150 160 170 180 IDLKDCFFTI PLAEQDCEKF AFTIPAINNK EPATRFQWKV LPQGMLNSPT ICQTFVGRAL 190 200 210 220 230 240 QPVRDKFSDC YIIHYFDDIL CAAETKDKLI DCYTFLQAEV ANAGLAIASD KIQTSTPFHY 250 260 270 280 290 300 LGMQIENRKI KPQKIEIRKD TLKTLNDFQK LLGDINWIRP TLGIPTYAMS NLFSILRGDS 310 320 330 340 350 360 DLNSKRMLTP EATKEIKLVE EKIQSAQINR IDPLAPLQLL IFATAHSPTG IIIQNTDLVE 370 380 390 400 410 420 WSFLPHSTVK TFTLYLDQIA TLIGPTRLRI IKLCGNDPDK IVVPLTKEQV RQAFINSGAW 430 440 450 460 470 480 QIGLANFVGI IDNHYPKTKI FQFLKLTTWI LPKITRREPL ENALTVFTDG SSNGKVAYTG 490 500 510 520 530 540 PKERVIKTPY QSAQRAELVA VITVLQDFDQ PINIISDSAY VVQATRDVET ALIKYSMDDQ 550 560 570 580 590 600 LNQLFNLLQQ TVRKRNFPFY ITHIRAHTNL PGPLTKANEQ ADLLVSSAFI KAQELHALTH 610 620 630 640 650 660 VNAAGLKNKF DVTWKQAKDI VQHCTQCQVL DLPTQEAGVN PEVCVLMHYG KWMSHMYLHL 670 680 690 700 710 720 GRLSYVHVTV DTYSHFMCAT CQTGESTSHV KKHLLSCFAV MGVPEKIKTD NGPGYCSKAF 730 740 750 760 770 780 QKFLSQWKIS HTTGIPYNSQ GQAIVERTNR TLKTQLVKQK EGGDSKECTT PQMQLNLALY 790 800 810 TLNFLNIYRN QTTTSAEHLT GKKNSPHEGK LI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome wide screening, cloning, chromosomal assignment and expression of full-length human endogenous retrovirus type K (HERV-K)." Toenjes R.R., Czauderna F., Kurth R. J. Virol. 73:9187-9195(1999) [PubMed: 10516026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Interferon-alpha induced endogenous superantigen: a model linking environment and autoimmunity." Stauffer Y., Marguerat S., Meylan F., Ucla C., Sutkowski N., Huber B.T., Pelet T., Conrad B. Immunity 15:591-601(2001) [PubMed: 11672541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The human immunodeficiency virus type 1 (HIV-1) Tat protein activates a replication-competent human endogenous retrovirus type K in astrocytes." Contreras-Galindo R.A., Chompre G., Gonzalez M., Noel R.J. Jr. Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 76-156.

Web resources

Human Endogenous Retrovirus Database (HERVd)

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y18890 Genomic DNA. Translation: CAB56603.1. Different initiation. AF333072 Genomic DNA. Translation: AAL60056.1. Different initiation. AL121985 Genomic DNA. No translation available. AY550203 Genomic DNA. Translation: AAS46612.1.
IPI	IPI00455074.
3D structure databases
SMR	Q9QC07. Positions 24-423, 590-629, 658-785.
ModBase	Search...
Proteomic databases
PRIDE	Q9QC07.
Phylogenomic databases
HOVERGEN	Q9QC07.
Enzyme and pathway databases
BRENDA	2.7.7.49. 247. 3.1.26.4. 247.
Gene expression databases
Genevestigator	Q9QC07.
Family and domain databases
InterPro	IPR001584. Integrase_cat-core. IPR003308. Integrase_Zn-bd_dom_N. IPR012337. PolynucTfrase_RNaseH_fold. IPR000477. Reverse_transcriptase. IPR002156. RNase_H. IPR010661. RVT_thumb. [Graphical view]
Pfam	PF02022. Integrase_Zn. 1 hit. PF00075. RnaseH. 1 hit. PF00665. rve. 1 hit. PF00078. RVT_1. 1 hit. PF06817. RVT_thumb. 1 hit. [Graphical view]
PROSITE	PS50994. INTEGRASE. 1 hit. PS50879. RNASE_H. 1 hit. PS50878. RT_POL. 1 hit. PS50876. ZF_INTEGRASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POK7_HUMAN

Accession

Primary (citable) accession number: Q9QC07
Secondary accession number(s): Q6QAI9, Q96PI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2004
Last sequence update:	September 13, 2004
Last modified:	January 19, 2010
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.