Q9QAZ8 (RDRP_SJNNV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: RNA-directed RNA polymerase Short name=RdRp EC=2.7.7.48 Alternative name(s): RNA replicase Short name=Protein A
Organism	Striped jack nervous necrosis virus (SjNNV) [Reference proteome]
Taxonomic identifier	35297 [NCBI]
Taxonomic lineage	Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Nodaviridae › Betanodavirus
Virus host	Dicentrarchus labrax (European seabass) (Morone labrax) [TaxID: 13489] Epinephelus akaara (Hong Kong grouper) (Serranus akaara) [TaxID: 215347] Hippoglossus hippoglossus (Atlantic halibut) (Pleuronectes hippoglossus) [TaxID: 8267] Lates calcarifer (Barramundi) (Holocentrus calcarifer) [TaxID: 8187] Oplegnathus fasciatus (barred knifejaw) [TaxID: 163134]

Protein attributes

Sequence length	983 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which replicates the viral genome composed of 2 RNA segments, RNA1 AND RNA2 Potential.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Sequence similarities	Belongs to the nodaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 983	983	RNA-directed RNA polymerase		PRO_0000222450
Regions
	Domain	579 – 701	123	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q9QAZ8 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F76890AA597F8BE1	FASTA	983	110,630
	10 20 30 40 50 60 MRRFEFELAR MSGAAFCVVT GYRLLTSKWL ADRVEDYRQR VIADRKQILR DAAVIRTSIQ 70 80 90 100 110 120 KQMELVRISV RKGHSHQEAA TERNSATDTM IGVVEKCGYE PYIISPSPRE KEYHGSRQFY 130 140 150 160 170 180 SLADFRQDYR RDEITDRHVI VMTDVDYYVD MHELVGLGVP ILLYTFQPST VSGEVKDGYF 190 200 210 220 230 240 TITDDHVHYR VAGGKDVRHR IWNYNQDTMF VRSKPRGFWA SLKQILRDIT GITALCGYLY 250 260 270 280 290 300 LKLGIAPFGD QVTLFTVDQF KMGEHRNIVS IVPFATCRSN LLKISEYGAE LDYMRYQQRN 310 320 330 340 350 360 NNANFNAVTY ISQEGPLISL GLEGNFASVQ LPLQDFENIR TAYELSKNNN LSDTVRRSAR 370 380 390 400 410 420 SCKEAAIIHK CLQAGCDLAS EVVHKPGELA RHYQALGDTY DIDPSEQGKC YAREYAPGPL 430 440 450 460 470 480 TQTAVFPSES RSNELATIDG RIAGPQAKAK SREHITPKMH KVARDFVRHL VPTAGLGRPY 490 500 510 520 530 540 PLTYVEEHQT KPLQRARNDA NRYHDEFTMI VKAFQKKEAY NAPNYPRNIS TVPHTQNVKL 550 560 570 580 590 600 SSYTYAFKEA VLQHVPWYMP THTPAEIAEA VQSLAASSTE LVETDYSKFD GTFLRFMREN 610 620 630 640 650 660 VEFAIYKRWV HLDHLTELST LLGNELQAPA VTRLGIKYDP DCSRLSGSAL TTDGNSIANA 670 680 690 700 710 720 FVSYLAGRQA GMDDDEAWTW IGIVYGDDGL RSGNVSDALL SKTASSLGFD LKIVNRAPRG 730 740 750 760 770 780 SPVTFLSRVY LDPWSSPASV QSPLRTLLKL HTTCDTQSDI EDVGWAKTQA YLVTDCLTPF 790 800 810 820 830 840 IGHWCRAYQR NCTARVVQYA DYNDIPFWVK NEDHVGNSWP QSDSVDWNDV VANELGLTTA 850 860 870 880 890 900 ELLKHLAALD AYTGPVSGLP RLTTSLDLEP KMPVALDGEV QAGPSQQPQT DKDGTSPTGD 910 920 930 940 950 960 RSAPRRARTA LQDADGRACR SRRSDRSPGK RDANVRDKRQ RRSTTPPRSR PSVPGPSSSG 970 980 RRTDGDRVRG GAARQRQRRR SPV

References

[1]	"Sequence of the non-structural protein gene encoded by RNA1 of striped jack nervous necrosis virus." Nagai T., Nishizawa T. J. Gen. Virol. 80:3019-3022(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Establishment of an infectious RNA transcription system for striped jack nervous necrosis virus, the type species of the betanodaviruses." Iwamoto T., Mise K., Mori K., Arimoto M., Nakai T., Okuno T. J. Gen. Virol. 82:2653-2662(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB025018 Genomic RNA. Translation: BAA84210.1. AB056571 Genomic RNA. Translation: BAB64329.1.
RefSeq	NP_599247.1. NC_003448.1.
3D structure databases
ProteinModelPortal	Q9QAZ8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	991144.
Family and domain databases
InterPro	IPR007094. RNA-dir_pol_PSvirus. IPR001788. Tymovirus_RNA-dep_RNA_pol. [Graphical view]
Pfam	PF00978. RdRP_2. 1 hit. [Graphical view]
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_SJNNV

Accession

Primary (citable) accession number: Q9QAZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program