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Q9Q9G3 (HEMA_HUTV) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hemagglutinin-esterase

Short name=HE protein
EC=3.1.1.53
Alternative name(s):
E3 glycoprotein
Gene names
Name:HE
OrganismHuman torovirus (HuTV)
Taxonomic identifier67605 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeTorovirinaeTorovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Structural protein that makes short spikes at the surface of the virus. Contains receptor binding and receptor-destroying activities. Mediates de-O-acetylation of N-acetyl-9-O-acetylneuraminic acid, which is probably the receptor determinant recognized by the virus on the surface of erythrocytes and susceptible cells. This receptor-destroying activity is important for virus release as it probably helps preventing self-aggregation and ensures the efficient spread of the progeny virus from cell to cell. May serve as a secondary viral attachment protein for initiating infection, the spike protein being the major one. Seems to be a 'luxury' protein that is not absolutely necessary for virus infection in culture. However, its presence in the virus may alter its pathogenicity. May become a target for both the humoral and the cellular branches of the immune system By similarity.

Catalytic activity

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Subcellular location

Virion membrane; Single-pass type I membrane protein Potential. Host cell membrane; Single-pass type I membrane protein Potential. Note: In infected cells becomes incorporated into the envelope of virions during virus assembly at the endoplasmic reticulum and cis Golgi. However, some may escape incorporation into virions and subsequently migrate to the cell surface By similarity.

Post-translational modification

N-glycosylated By similarity.

Sequence similarities

Belongs to the influenza type C/coronaviruses hemagglutinin-esterase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1414 Potential
Chain15 – 416402Hemagglutinin-esterase
PRO_0000045400

Regions

Topological domain15 – 393379Virion surface Potential
Transmembrane394 – 41421Helical; Potential
Topological domain415 – 4162Intravirion Potential
Region4 – 121118Esterase domain first part By similarity
Region122 – 263142Receptor binding By similarity
Region264 – 379116Esterase domain second part By similarity
Compositional bias64 – 674Poly-Ser
Compositional bias402 – 4054Poly-Val

Sites

Active site371Nucleophile By similarity
Active site2231Charge relay system By similarity
Active site3281Charge relay system By similarity

Amino acid modifications

Glycosylation591N-linked (GlcNAc...); by host Potential
Glycosylation761N-linked (GlcNAc...); by host Potential
Glycosylation2571N-linked (GlcNAc...); by host Potential
Glycosylation2781N-linked (GlcNAc...); by host Potential
Glycosylation2941N-linked (GlcNAc...); by host Potential
Glycosylation3221N-linked (GlcNAc...); by host Potential
Glycosylation3431N-linked (GlcNAc...); by host Potential
Disulfide bond41 ↔ 57 By similarity
Disulfide bond108 ↔ 156 By similarity
Disulfide bond192 ↔ 273 By similarity
Disulfide bond200 ↔ 246 By similarity
Disulfide bond304 ↔ 309 By similarity
Disulfide bond346 ↔ 371 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9Q9G3 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 13607F00B989ACD8

FASTA41646,534
        10         20         30         40         50         60 
MLSLILFFPS FAFAVTPVTP YFGPLYITFN CCLFGDSRSD CTKVQSPMSL DNPQNFCPNF 

        70         80         90        100        110        120 
SLKSSSSMFF SIHYNNHSSL VLFDNFNCRI EKVYYNGVNL SPRNQYSCYD EGVDSYMELK 

       130        140        150        160        170        180 
TSFNIKLNQM ATILRCIKLI QLKARSSFTT LQDVVCRTNK YLPNNPTFAL LSDTVPTWVQ 

       190        200        210        220        230        240 
FVLPDLSGKT ICIKYLVPFC HLNHGCFTAG SSCPPFGVSY VSDSFNYGFN DATPYIGLAE 

       250        260        270        280        290        300 
SHDNVCDYLF VEAGTHNASI VGNFLFYPTK SYCFNTMNFT VPVQAIQSIW SEGNESDDAI 

       310        320        330        340        350        360 
AEACKPPFCI YYSKTTPYTV TNGSNADHRD DEVRMMVRGL LYNSSCISAQ GSTPLALYST 

       370        380        390        400        410 
AMLYAPIYGS CPQYVKLFDT SGSESVDVIS SSYFVATWVL LVVVVILIFV IISFFC 

« Hide

References

[1]"The novel hemagglutinin-esterase genes of human torovirus and Breda virus."
Duckmanton L., Tellier R., Richardson C., Petric M.
Virus Res. 64:137-149(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF159585 mRNA. Translation: AAF00614.1.

3D structure databases

ProteinModelPortalQ9Q9G3.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
[Graphical view]
PfamPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
[Graphical view]
SUPFAMSSF49818. SSF49818. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHEMA_HUTV
AccessionPrimary (citable) accession number: Q9Q9G3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families