Q9Q8Z2 (NTP1_RFVKA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside triphosphatase I
EC=3.6.1.15

Alternative name(s):
NPH-I
Nucleoside triphosphate phosphohydrolase I
Short name=NPH I

Gene names

Name:	NPH1
Ordered Locus Names:	s086L

Organism

Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain Kasza))

Taxonomic identifier

10272 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Leporipoxvirus

Virus host

Oryctolagus cuniculus (Rabbit) [TaxID: 9986]

Protein attributes

Sequence length	632 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent ATPase required for providing the needed energy to achieve the termination of early transcripts. Acts in concert with the RAP94 subunit of the virion RNA polymerase and the capping enzyme/VTF to catalyze release of UUUUUNU-containing nascent RNA from the elongation complex. NPH-I must bind ssDNA in order to exhibit ATPase activity By similarity.
Catalytic activity	NTP + H2O = NDP + phosphate.
Subunit structure	Monomer. Interacts (via C-terminus) with RAP94 (via N-terminus). Interacts with the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity.
Subcellular location	Virion By similarity. Note: Virion core enzyme By similarity.
Sequence similarities	Belongs to the helicase family. NPH I subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Virion
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 632	632	Nucleoside triphosphatase I		PRO_0000099096
Regions
	Domain	42 – 204	163	Helicase ATP-binding
	Domain	367 – 532	166	Helicase C-terminal
	Nucleotide binding	55 – 62	8	ATP By similarity
	Region	457 – 524	68	Binding to the cap-specific mRNA (nucleoside-2'-O-)-methyltransferase By similarity
	Motif	141 – 144	4	DEXH box
Experimental info
	Sequence conflict	404	1	F → V Ref.2
	Sequence conflict	496	1	H → T Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9Q8Z2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2579BE4806A92F06	FASTA	632	72,883
	10 20 30 40 50 60 MSVHHAAYID YALRVTENMT DVMTGSDTVT LKSYQHFVSR VFLGLDKMHS LLLFHETGVG 70 80 90 100 110 120 KTITTVFILK HLKDVYTNWI IILLVKKALV EDPWTYSITK YAPEILKDCI FITYDDKNFH 130 140 150 160 170 180 NKFFTNIKTI SSRSRLCIII DECHNFISKS IVKEDGKQRP TKSVYNYLLK NVALHHHKLI 190 200 210 220 230 240 CLSATPIVNS VKEFIMLVNL LRPKILNNIS LFENKRLINE SELINKLGAI CSYIVTNEFS 250 260 270 280 290 300 IFDDVEGSAT FAKKNVYFQY VNMTQKQEQV YQKAKSAELK AGTSSFRIYR RMAATFTFDT 310 320 330 340 350 360 FLDKTDKTPE EIVNEQVTLY KDFEKFIKTK KFSDHALSHF KKGQSLNGTS SADDISFLNE 370 380 390 400 410 420 LREHSCKFTD VCLRILASPG KCLVFEPFVN QSGINILLLY FSAFNISYIE FSSRTKNTRV 430 440 450 460 470 480 QSVAEFNKRE NTNGDLIKTC VFSLSGGEGI SFFSINDIFI LDMTWNEASL RQIIGRAIRL 490 500 510 520 530 540 NSHVLTPEHR RYVYVHFIIA RLSNGDPTVD EDLLDIIRNK SKEFTQLFKV FKHTSIEWIY 550 560 570 580 590 600 EHQTNFSPVD DESGWGALIS RSIDENPTTK RVPRIARGQN IWYSHSNRMI TVYKGFKTDD 610 620 630 GRLFDSDGNF IQTIQANPVI KIHNNKLVYV LD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of shope (Rabbit) fibroma virus." Willer D.O., McFadden G., Evans D.H. Virology 264:319-343(1999) [PubMed: 10562495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Sequence and analysis of the BamHI 'D' fragment of Shope fibroma virus: comparison with similar regions of related poxviruses." Strayer D.S., Jerng H.H. Virus Res. 25:117-132(1992) [PubMed: 1329373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-632.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF170722 Genomic DNA. Translation: AAF17970.1.
RefSeq	NP_051975.1. NC_001266.1.
3D structure databases
ProteinModelPortal	Q9Q8Z2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1486931.
Phylogenomic databases
ProtClustDB	CLSP2509799.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR001650. Helicase_C. IPR013676. NPHI_C. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF08469. NPHI_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP1_RFVKA

Accession

Primary (citable) accession number: Q9Q8Z2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program