Reviewed, UniProtKB/Swiss-Prot Q9Q8Q2 (NTP2_MYXVL)

Names and origin

Protein names

Recommended name:
    Nucleoside triphosphatase II
    EC=3.6.1.15
Alternative name(s):
    Nucleoside triphosphate phosphohydrolase II
      Short name=NPH II
    RNA helicase m44R

Gene names

Name:	NPH2
Ordered Locus Names:	m044R

Organism

Myxoma virus (strain Lausanne) (MYXV)

Taxonomic identifier

31530 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Leporipoxvirus

Virus host

Oryctolagus cuniculus (Rabbit) [TaxID: 9986]

Protein attributes

Sequence length	678 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential for viral replication. Plays an important role during transcription of early mRNAs, presumably by preventing R-loop formation behind the elongating RNA polymerase. Acts as NTP-dependent helicase that catalyzes unidirectional unwinding of 3'tailed duplex RNAs. Might also play a role in the export of newly synthesized mRNA chains out of the core into the cytoplasm. Required for propagation of viral particles By similarity.
Catalytic activity	NTP + H2O = NDP + phosphate.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 678	678	Nucleoside triphosphatase II		PRO_0000055190
Regions
	Domain	175 – 351	177	Helicase ATP-binding
	Domain	371 – 546	176	Helicase C-terminal
	Nucleotide binding	188 – 195	8	ATP By similarity
	Motif	300 – 303	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	Q9Q8Q2-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E30ED17F105A22F7	FASTA	678	77,789
	10 20 30 40 50 60 MENHLPNIFY FPNCVTAFPY RYTQKELDDM KPVDRERFKY AVFPLIKHRW CRAYVVRDNH 70 80 90 100 110 120 TFKLNVETSK RLRRVAYPTL VPLVGVNAAL REYVMEDGTK ISFECYSYLI CKRTTSLHDV 130 140 150 160 170 180 DDSTIRGLVE GGNQLNIFTN SVGSVTNTVG IFGNPNPFAK VPLKSLHPSM QCKIFTSWAR 190 200 210 220 230 240 RVPVVLTGDT GVGKTSQVPK LLLWFNYLFG GFTDLSTLTF EVQEKPIVLS LPRVALVKLH 250 260 270 280 290 300 SETLLTSLGF EEIHGSPVSL KFGNMQERFV NTRFSRYGIV FSTHKITLNT LFKYSTVILD 310 320 330 340 350 360 EVHEHDQTGD IIIAVCRKYI RKLDSLFLMT ATLEDDRRRI EEFFAESVFV HIPGGTLFSI 370 380 390 400 410 420 SEAYVKNSND PLNRFMYIEE EKRNLANAIK TYTPPKQSSG IVFVSTVSQC EAYKQYLSER 430 440 450 460 470 480 LPYKFYIIHG KVQNINDVLS DIYDNDGVSI IISTPYLESS VTVRNATHVY DTGRVYIPSP 490 500 510 520 530 540 YGGCESFISK SMRDQRKGRV GRVNPGMYVY FYNVSELRPI KRIDFEFLHN YVLYAKVFDL 550 560 570 580 590 600 QLPEDLFVKP TNVTRLHDVI EYIRSFDISD DVWTRLLSSY YIHILEYAKV YARGGSGALA 610 620 630 640 650 660 LDSFERTGNL TDDALDAIKS LNMRAKILSH KKASAHTYAL RCKLLFGVYA GKVFTVYHKR 670 PLTGYITMIA EHSFIPDY

References

[1]	"The complete DNA sequence of myxoma virus." Cameron C., Hota-Mitchell S., Chen L., Barrett J.W., Cao J.-X., Macaulay C., Willer D.O., Evans D.H., McFadden G. Virology 264:298-318(1999) [PubMed: 10562494] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF170726 Genomic DNA. Translation: AAF14932.1.
RefSeq	NP_051758.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	932096.
Enzyme and pathway databases
BRENDA	3.6.1.15. 321803.
Family and domain databases
InterPro	IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR014021. Helicase_SF1/SF2_ATP-bd. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTP2_MYXVL

Accession

Primary (citable) accession number: Q9Q8Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)