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Q9PY93

- NSP2_ROTHC

UniProt

Q9PY93 - NSP2_ROTHC

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Protein
Non-structural protein 2
Gene
N/A
Organism
Rotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments By similarity.

Cofactori

Magnesium for NTPase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi153 – 1531Magnesium Reviewed prediction
Metal bindingi171 – 1711Magnesium Reviewed prediction
Active sitei222 – 2221For NTPase activity By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. hydrolase activity, acting on acid anhydrides Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
Alternative name(s):
NCVP3
Non-structural RNA-binding protein 35
Short name:
NS35
OrganismiRotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Taxonomic identifieri31567 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007664: Genome

Subcellular locationi

Host cytoplasm Reviewed prediction
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi185 – 1851K → A: Loss of NTPase activity and dsRNA synthesis. No effect on octamerization. 1 Publication
Mutagenesisi222 – 2221H → A: Loss of NTPase activity and dsRNA synthesis. No effect on octamerization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Non-structural protein 2
PRO_0000367815Add
BLAST

Interactioni

Subunit structurei

Homooctamer By similarity. Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63
Beta strandi8 – 114
Beta strandi13 – 164
Beta strandi18 – 214
Helixi24 – 329
Helixi37 – 393
Beta strandi47 – 493
Helixi55 – 606
Helixi74 – 9017
Helixi94 – 963
Helixi99 – 1035
Helixi108 – 11912
Helixi124 – 1263
Helixi129 – 13911
Helixi149 – 1513
Beta strandi157 – 1604
Beta strandi162 – 1709
Beta strandi175 – 1773
Beta strandi182 – 19211
Helixi196 – 20914
Beta strandi213 – 2164
Beta strandi218 – 22710
Helixi228 – 2303
Helixi231 – 24313
Helixi264 – 27512
Helixi279 – 2824
Helixi285 – 2884
Helixi293 – 30715

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GU0X-ray2.80A/B1-312[»]
ProteinModelPortaliQ9PY93.
SMRiQ9PY93. Positions 2-309.

Miscellaneous databases

EvolutionaryTraceiQ9PY93.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni202 – 23837RNA-binding Reviewed prediction
Add
BLAST

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9PY93-1 [UniParc]FASTAAdd to Basket

« Hide

MAELACFVSF SLTEDKVVWY PINKKAVQTM LCAKVEKDQR SNYYDTILYG    50
VAPPPEFRNR FKTNERYGLD YESDQYTELV NLLADTLNMV SMPTEKFQFD 100
IVKTVVQVRH LENLLCRIKD VNDILNANVK LRVKAVMIAC NLVNETETTP 150
LTESNDIVYQ DSYFTITKLD YSNHKLLPLM ADEYKITINT KTDIPDRNQT 200
AFAAYIRYNF NKFAAISHGK RHWRLVLHSQ LMSHAERLDR KIKSDKKHGR 250
QFSYDDGDMA FVHPGWKTCI GQLCGGTTFE VAKTSLYSIK PSKTVRTATN 300
KIESDLISMV GN 312
Length:312
Mass (Da):35,915
Last modified:May 1, 2000 - v1
Checksum:iD2E6C11B6582637F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ132205 Genomic RNA. Translation: CAB52753.1.
RefSeqiYP_392488.1. NC_007545.1.

Genome annotation databases

GeneIDi3773135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ132205 Genomic RNA. Translation: CAB52753.1 .
RefSeqi YP_392488.1. NC_007545.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2GU0 X-ray 2.80 A/B 1-312 [» ]
ProteinModelPortali Q9PY93.
SMRi Q9PY93. Positions 2-309.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3773135.

Miscellaneous databases

EvolutionaryTracei Q9PY93.

Family and domain databases

Gene3Di 3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProi IPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view ]
Pfami PF02509. Rota_NS35. 1 hit.
[Graphical view ]
SUPFAMi SSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of human group C rotavirus genes 6, 7 and 9."
    James V.L., Lambden P.R., Deng Y., Caul E.O., Clarke I.N.
    J. Gen. Virol. 80:3181-3187(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Structure-function analysis of rotavirus NSP2 octamer by using a novel complementation system."
    Taraporewala Z.F., Jiang X., Vasquez-Del Carpio R., Jayaram H., Prasad B.V.V., Patton J.T.
    J. Virol. 80:7984-7994(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), MUTAGENESIS OF LYS-185 AND HIS-222.

Entry informationi

Entry nameiNSP2_ROTHC
AccessioniPrimary (citable) accession number: Q9PY93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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