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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments (By similarity).By similarity

Cofactori

Mg2+Note: Magnesium is required for NTPase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi153 – 1531MagnesiumSequence Analysis
Metal bindingi171 – 1711MagnesiumSequence Analysis
Active sitei222 – 2221For NTPase activityBy similarity

GO - Molecular functioni

  1. hydrolase activity, acting on acid anhydrides Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. nucleotide binding Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
Alternative name(s):
NCVP3
Non-structural RNA-binding protein 35
Short name:
NS35
OrganismiRotavirus C (isolate Human/United Kingdom/Bristol/1989) (RV-C)
Taxonomic identifieri31567 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007664 Componenti: Genome

Subcellular locationi

  1. Host cytoplasm Curated

  2. Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi185 – 1851K → A: Loss of NTPase activity and dsRNA synthesis. No effect on octamerization. 1 Publication
Mutagenesisi222 – 2221H → A: Loss of NTPase activity and dsRNA synthesis. No effect on octamerization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Non-structural protein 2PRO_0000367815Add
BLAST

Interactioni

Subunit structurei

Homooctamer (By similarity). Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories.By similarity

Structurei

Secondary structure

1
312
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Beta strandi8 – 114Combined sources
Beta strandi13 – 164Combined sources
Beta strandi18 – 214Combined sources
Helixi24 – 329Combined sources
Helixi37 – 393Combined sources
Beta strandi47 – 493Combined sources
Helixi55 – 606Combined sources
Helixi74 – 9017Combined sources
Helixi94 – 963Combined sources
Helixi99 – 1035Combined sources
Helixi108 – 11912Combined sources
Helixi124 – 1263Combined sources
Helixi129 – 13911Combined sources
Helixi149 – 1513Combined sources
Beta strandi157 – 1604Combined sources
Beta strandi162 – 1709Combined sources
Beta strandi175 – 1773Combined sources
Beta strandi182 – 19211Combined sources
Helixi196 – 20914Combined sources
Beta strandi213 – 2164Combined sources
Beta strandi218 – 22710Combined sources
Helixi228 – 2303Combined sources
Helixi231 – 24313Combined sources
Helixi264 – 27512Combined sources
Helixi279 – 2824Combined sources
Helixi285 – 2884Combined sources
Helixi293 – 30715Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GU0X-ray2.80A/B1-312[»]
ProteinModelPortaliQ9PY93.
SMRiQ9PY93. Positions 2-309.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9PY93.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni202 – 23837RNA-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.Curated

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9PY93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELACFVSF SLTEDKVVWY PINKKAVQTM LCAKVEKDQR SNYYDTILYG
60 70 80 90 100
VAPPPEFRNR FKTNERYGLD YESDQYTELV NLLADTLNMV SMPTEKFQFD
110 120 130 140 150
IVKTVVQVRH LENLLCRIKD VNDILNANVK LRVKAVMIAC NLVNETETTP
160 170 180 190 200
LTESNDIVYQ DSYFTITKLD YSNHKLLPLM ADEYKITINT KTDIPDRNQT
210 220 230 240 250
AFAAYIRYNF NKFAAISHGK RHWRLVLHSQ LMSHAERLDR KIKSDKKHGR
260 270 280 290 300
QFSYDDGDMA FVHPGWKTCI GQLCGGTTFE VAKTSLYSIK PSKTVRTATN
310
KIESDLISMV GN
Length:312
Mass (Da):35,915
Last modified:May 1, 2000 - v1
Checksum:iD2E6C11B6582637F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132205 Genomic RNA. Translation: CAB52753.1.
RefSeqiYP_392488.1. NC_007545.1.

Genome annotation databases

GeneIDi3773135.
KEGGivg:3773135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132205 Genomic RNA. Translation: CAB52753.1.
RefSeqiYP_392488.1. NC_007545.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GU0X-ray2.80A/B1-312[»]
ProteinModelPortaliQ9PY93.
SMRiQ9PY93. Positions 2-309.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3773135.
KEGGivg:3773135.

Miscellaneous databases

EvolutionaryTraceiQ9PY93.

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular characterization of human group C rotavirus genes 6, 7 and 9."
    James V.L., Lambden P.R., Deng Y., Caul E.O., Clarke I.N.
    J. Gen. Virol. 80:3181-3187(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Structure-function analysis of rotavirus NSP2 octamer by using a novel complementation system."
    Taraporewala Z.F., Jiang X., Vasquez-Del Carpio R., Jayaram H., Prasad B.V.V., Patton J.T.
    J. Virol. 80:7984-7994(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), MUTAGENESIS OF LYS-185 AND HIS-222.

Entry informationi

Entry nameiNSP2_ROTHC
AccessioniPrimary (citable) accession number: Q9PY93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 1, 2000
Last modified: April 1, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.