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Protein

Non-structural protein 2

Gene
N/A
Organism
Rotavirus C (isolate RVC/Human/United Kingdom/Bristol/1989) (RV-C)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in genome replication and packaging. Plays a crucial role, together with NSP5, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where replication intermediates are assembled and RNA replication takes place. Displays ssRNA binding, NTPase, RNA triphosphatase (RTPase) and ATP-independent helix-unwinding activity activities. The unwiding activity may prepare and organize plus-strand RNAs for packaging and replication by removing interfering secondary structures. Unlike typical helicases, NSP2 requires neither a divalent cation nor a nucleotide energy source for helix destabilization. The RTPase activity may account for the absence of the 5'-terminal gamma-phosphate on the minus-strands of dsRNA genome segments (By similarity).By similarity

Cofactori

Mg2+Note: Magnesium is required for NTPase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi153MagnesiumSequence analysis1
Metal bindingi171MagnesiumSequence analysis1
Active sitei222For NTPase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 2 (EC:3.6.4.-)
Short name:
NSP2
Alternative name(s):
NCVP3
Non-structural RNA-binding protein 35
Short name:
NS35
OrganismiRotavirus C (isolate RVC/Human/United Kingdom/Bristol/1989) (RV-C)
Taxonomic identifieri31567 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007664 Componenti: Genome

Subcellular locationi

  • Host cytoplasm Curated

  • Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi185K → A: Loss of NTPase activity and dsRNA synthesis. No effect on octamerization. 1 Publication1
Mutagenesisi222H → A: Loss of NTPase activity and dsRNA synthesis. No effect on octamerization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003678151 – 312Non-structural protein 2Add BLAST312

Interactioni

Subunit structurei

Homooctamer (By similarity). Interacts with VP1; this interaction is weak. Interacts with NSP5; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories.By similarity

Structurei

Secondary structure

1312
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi8 – 11Combined sources4
Beta strandi13 – 16Combined sources4
Beta strandi18 – 21Combined sources4
Helixi24 – 32Combined sources9
Helixi37 – 39Combined sources3
Beta strandi47 – 49Combined sources3
Helixi55 – 60Combined sources6
Helixi74 – 90Combined sources17
Helixi94 – 96Combined sources3
Helixi99 – 103Combined sources5
Helixi108 – 119Combined sources12
Helixi124 – 126Combined sources3
Helixi129 – 139Combined sources11
Helixi149 – 151Combined sources3
Beta strandi157 – 160Combined sources4
Beta strandi162 – 170Combined sources9
Beta strandi175 – 177Combined sources3
Beta strandi182 – 192Combined sources11
Helixi196 – 209Combined sources14
Beta strandi213 – 216Combined sources4
Beta strandi218 – 227Combined sources10
Helixi228 – 230Combined sources3
Helixi231 – 243Combined sources13
Helixi264 – 275Combined sources12
Helixi279 – 282Combined sources4
Helixi285 – 288Combined sources4
Helixi293 – 307Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GU0X-ray2.80A/B1-312[»]
ProteinModelPortaliQ9PY93.
SMRiQ9PY93.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9PY93.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni202 – 238RNA-bindingSequence analysisAdd BLAST37

Sequence similaritiesi

Belongs to the rotavirus NSP2 family.Curated

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9PY93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELACFVSF SLTEDKVVWY PINKKAVQTM LCAKVEKDQR SNYYDTILYG
60 70 80 90 100
VAPPPEFRNR FKTNERYGLD YESDQYTELV NLLADTLNMV SMPTEKFQFD
110 120 130 140 150
IVKTVVQVRH LENLLCRIKD VNDILNANVK LRVKAVMIAC NLVNETETTP
160 170 180 190 200
LTESNDIVYQ DSYFTITKLD YSNHKLLPLM ADEYKITINT KTDIPDRNQT
210 220 230 240 250
AFAAYIRYNF NKFAAISHGK RHWRLVLHSQ LMSHAERLDR KIKSDKKHGR
260 270 280 290 300
QFSYDDGDMA FVHPGWKTCI GQLCGGTTFE VAKTSLYSIK PSKTVRTATN
310
KIESDLISMV GN
Length:312
Mass (Da):35,915
Last modified:May 1, 2000 - v1
Checksum:iD2E6C11B6582637F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132205 Genomic RNA. Translation: CAB52753.1.
RefSeqiYP_392488.1. NC_007545.1.

Genome annotation databases

GeneIDi3773135.
KEGGivg:3773135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132205 Genomic RNA. Translation: CAB52753.1.
RefSeqiYP_392488.1. NC_007545.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GU0X-ray2.80A/B1-312[»]
ProteinModelPortaliQ9PY93.
SMRiQ9PY93.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3773135.
KEGGivg:3773135.

Miscellaneous databases

EvolutionaryTraceiQ9PY93.

Family and domain databases

Gene3Di3.30.428.20. 1 hit.
3.90.1400.10. 1 hit.
InterProiIPR003668. Rotavirus_NSP2.
IPR024076. Rotavirus_NSP2_C-term.
IPR024068. Rotavirus_NSP2_N.
[Graphical view]
PfamiPF02509. Rota_NS35. 1 hit.
[Graphical view]
SUPFAMiSSF75347. SSF75347. 1 hit.
SSF75574. SSF75574. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNSP2_ROTHC
AccessioniPrimary (citable) accession number: Q9PY93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.