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Q9PY83

- MUNS_REOVL

UniProt

Q9PY83 - MUNS_REOVL

Protein

Protein mu-NS

Gene

M3

Organism
Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions.1 Publication

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein mu-NS
    Short name:
    MuNS
    Gene namesi
    Name:M3
    OrganismiReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
    Taxonomic identifieri10884 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
    Virus hostiMammalia [TaxID: 40674]
    ProteomesiUP000007253: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 721721Protein mu-NSPRO_0000345003Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.By similarity

    Interactioni

    Subunit structurei

    Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions.2 Publications

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 1313Interaction with sigma-NSAdd
    BLAST
    Regioni14 – 4027Interaction with mu-2Add
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili523 – 56038Sequence AnalysisAdd
    BLAST
    Coiled coili628 – 68659Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the orthoreovirus mu-NS protein family.Curated

    Keywords - Domaini

    Coiled coil

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform mu-NS (identifier: Q9PY83-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASFKGFSVN TVPVSKAKRD ISSLAATPGI RSQPFTPSVD MSQSREFLTK    50
    AIEQGSMSIP YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL 100
    DCLPQLEQAM AFVASPESFQ ASDVAKRFAI KPGMSLQDAI TAFINFVSAM 150
    LKMTVTRQNF DVIVAEIERL ASTSVSVRTE EAKVADEELM LFGLDHRGPQ 200
    QLDISNAKGI MKAADIQATH DVHLAPGVGN IDPEIYNEGR FMFMQHKPLA 250
    ADQSYFTLET ADYFKIYPTY DEHDSRMADQ KQSGLILCTK DEVLAEQTIF 300
    KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD 350
    LYANTQVVSI TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG 400
    VSLCALLMDE KLSVLNSACR MTLRSLMKTG RDADAHRAFQ RVLSQGYASL 450
    MCYYHPSRKL AYGEVLFLER SSDMVDGIKL QLDASRQCHE CPVLQQKVVE 500
    LEKQIIMQKS IQSDPTPMAL QPLLSQLREL SSEVTRLQME LSRTQSLNAQ 550
    LEADAKSAQA CSLDMYLRHH TCINGHTKED ELLDAVRVAP DVRKEIMEKR 600
    GEVRRGWCER ISKEAAAKCQ TVIDDLTQMN GKQAREITEL RESAENYEKQ 650
    IAELVGTITQ NQMTYQQELQ ALVAKNVELD TMNQRQAKSL RITPSLLSAT 700
    PIDSVDGAAD LIDFSVPTDE L 721
    Length:721
    Mass (Da):80,166
    Last modified:May 1, 2000 - v1
    Checksum:iC84FED8A68ED53C9
    GO
    Isoform mu-NSC (identifier: Q9PY83-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-40: Missing.

    Note: It is unsure whether Met-41 is the initiator.

    Show »
    Length:681
    Mass (Da):75,989
    Checksum:i5EC190C3BFCF6F2E
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4040Missing in isoform mu-NSC. CuratedVSP_034870Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF174382 mRNA. Translation: AAF13169.1.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF174382 mRNA. Translation: AAF13169.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein."
      McCutcheon A.M., Broering T.J., Nibert M.L.
      Virology 264:16-24(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
    2. "Reovirus sigma NS protein localizes to inclusions through an association requiring the mu NS amino terminus."
      Miller C.L., Broering T.J., Parker J.S.L., Arnold M.M., Nibert M.L.
      J. Virol. 77:4566-4576(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PROTEIN SIGMA-NS AND PROTEIN MU-2.
    3. "Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions."
      Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L.
      J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PROTEIN LAMBDA-1; PROTEIN LAMBDA-2 AND PROTEIN SIGMA-2.

    Entry informationi

    Entry nameiMUNS_REOVL
    AccessioniPrimary (citable) accession number: Q9PY83
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 22, 2008
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3