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Q9PY83

- MUNS_REOVL

UniProt

Q9PY83 - MUNS_REOVL

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Protein

Protein mu-NS

Gene
M3
Organism
Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions.1 Publication

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mu-NS
Short name:
MuNS
Gene namesi
Name:M3
OrganismiReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Taxonomic identifieri10884 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000007253: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Protein mu-NSPRO_0000345003Add
BLAST

Post-translational modificationi

The N-terminus is blocked By similarity.

Interactioni

Subunit structurei

Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions.2 Publications

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1313Interaction with sigma-NSAdd
BLAST
Regioni14 – 4027Interaction with mu-2Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili523 – 56038 Reviewed predictionAdd
BLAST
Coiled coili628 – 68659 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform mu-NS (identifier: Q9PY83-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MASFKGFSVN TVPVSKAKRD ISSLAATPGI RSQPFTPSVD MSQSREFLTK    50
AIEQGSMSIP YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL 100
DCLPQLEQAM AFVASPESFQ ASDVAKRFAI KPGMSLQDAI TAFINFVSAM 150
LKMTVTRQNF DVIVAEIERL ASTSVSVRTE EAKVADEELM LFGLDHRGPQ 200
QLDISNAKGI MKAADIQATH DVHLAPGVGN IDPEIYNEGR FMFMQHKPLA 250
ADQSYFTLET ADYFKIYPTY DEHDSRMADQ KQSGLILCTK DEVLAEQTIF 300
KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD 350
LYANTQVVSI TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG 400
VSLCALLMDE KLSVLNSACR MTLRSLMKTG RDADAHRAFQ RVLSQGYASL 450
MCYYHPSRKL AYGEVLFLER SSDMVDGIKL QLDASRQCHE CPVLQQKVVE 500
LEKQIIMQKS IQSDPTPMAL QPLLSQLREL SSEVTRLQME LSRTQSLNAQ 550
LEADAKSAQA CSLDMYLRHH TCINGHTKED ELLDAVRVAP DVRKEIMEKR 600
GEVRRGWCER ISKEAAAKCQ TVIDDLTQMN GKQAREITEL RESAENYEKQ 650
IAELVGTITQ NQMTYQQELQ ALVAKNVELD TMNQRQAKSL RITPSLLSAT 700
PIDSVDGAAD LIDFSVPTDE L 721
Length:721
Mass (Da):80,166
Last modified:May 1, 2000 - v1
Checksum:iC84FED8A68ED53C9
GO
Isoform mu-NSC (identifier: Q9PY83-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Note: It is unsure whether Met-41 is the initiator.

Show »
Length:681
Mass (Da):75,989
Checksum:i5EC190C3BFCF6F2E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4040Missing in isoform mu-NSC. VSP_034870Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF174382 mRNA. Translation: AAF13169.1.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF174382 mRNA. Translation: AAF13169.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein."
    McCutcheon A.M., Broering T.J., Nibert M.L.
    Virology 264:16-24(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
  2. "Reovirus sigma NS protein localizes to inclusions through an association requiring the mu NS amino terminus."
    Miller C.L., Broering T.J., Parker J.S.L., Arnold M.M., Nibert M.L.
    J. Virol. 77:4566-4576(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PROTEIN SIGMA-NS AND PROTEIN MU-2.
  3. "Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions."
    Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L.
    J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PROTEIN LAMBDA-1; PROTEIN LAMBDA-2 AND PROTEIN SIGMA-2.

Entry informationi

Entry nameiMUNS_REOVL
AccessioniPrimary (citable) accession number: Q9PY83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: May 1, 2000
Last modified: April 3, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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