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Protein

Protein mu-NS

Gene

M3

Organism
Reovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions.1 Publication

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein mu-NS
Short name:
MuNS
Gene namesi
Name:M3
OrganismiReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1)
Taxonomic identifieri10884 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000007253 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Protein mu-NSPRO_0000345003Add
BLAST

Post-translational modificationi

The N-terminus is blocked.By similarity

Interactioni

Subunit structurei

Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions.2 Publications

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1313Interaction with sigma-NSAdd
BLAST
Regioni14 – 4027Interaction with mu-2Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili523 – 56038Sequence AnalysisAdd
BLAST
Coiled coili628 – 68659Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the orthoreovirus mu-NS protein family.Curated

Keywords - Domaini

Coiled coil

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform mu-NS (identifier: Q9PY83-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASFKGFSVN TVPVSKAKRD ISSLAATPGI RSQPFTPSVD MSQSREFLTK
60 70 80 90 100
AIEQGSMSIP YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL
110 120 130 140 150
DCLPQLEQAM AFVASPESFQ ASDVAKRFAI KPGMSLQDAI TAFINFVSAM
160 170 180 190 200
LKMTVTRQNF DVIVAEIERL ASTSVSVRTE EAKVADEELM LFGLDHRGPQ
210 220 230 240 250
QLDISNAKGI MKAADIQATH DVHLAPGVGN IDPEIYNEGR FMFMQHKPLA
260 270 280 290 300
ADQSYFTLET ADYFKIYPTY DEHDSRMADQ KQSGLILCTK DEVLAEQTIF
310 320 330 340 350
KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD
360 370 380 390 400
LYANTQVVSI TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG
410 420 430 440 450
VSLCALLMDE KLSVLNSACR MTLRSLMKTG RDADAHRAFQ RVLSQGYASL
460 470 480 490 500
MCYYHPSRKL AYGEVLFLER SSDMVDGIKL QLDASRQCHE CPVLQQKVVE
510 520 530 540 550
LEKQIIMQKS IQSDPTPMAL QPLLSQLREL SSEVTRLQME LSRTQSLNAQ
560 570 580 590 600
LEADAKSAQA CSLDMYLRHH TCINGHTKED ELLDAVRVAP DVRKEIMEKR
610 620 630 640 650
GEVRRGWCER ISKEAAAKCQ TVIDDLTQMN GKQAREITEL RESAENYEKQ
660 670 680 690 700
IAELVGTITQ NQMTYQQELQ ALVAKNVELD TMNQRQAKSL RITPSLLSAT
710 720
PIDSVDGAAD LIDFSVPTDE L
Length:721
Mass (Da):80,166
Last modified:April 30, 2000 - v1
Checksum:iC84FED8A68ED53C9
GO
Isoform mu-NSC (identifier: Q9PY83-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Note: It is unsure whether Met-41 is the initiator.

Show »
Length:681
Mass (Da):75,989
Checksum:i5EC190C3BFCF6F2E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4040Missing in isoform mu-NSC. CuratedVSP_034870Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF174382 mRNA. Translation: AAF13169.1.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF174382 mRNA. Translation: AAF13169.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein."
    McCutcheon A.M., Broering T.J., Nibert M.L.
    Virology 264:16-24(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
  2. "Reovirus sigma NS protein localizes to inclusions through an association requiring the mu NS amino terminus."
    Miller C.L., Broering T.J., Parker J.S.L., Arnold M.M., Nibert M.L.
    J. Virol. 77:4566-4576(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PROTEIN SIGMA-NS AND PROTEIN MU-2.
  3. "Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions."
    Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L.
    J. Virol. 78:1882-1892(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PROTEIN LAMBDA-1; PROTEIN LAMBDA-2 AND PROTEIN SIGMA-2.

Entry informationi

Entry nameiMUNS_REOVL
AccessioniPrimary (citable) accession number: Q9PY83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 2008
Last sequence update: April 30, 2000
Last modified: January 6, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.