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Q9PY83 (MUNS_REOVL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein mu-NS

Short name=MuNS
Gene names
Name:M3
OrganismReovirus type 1 (strain Lang) (T1L) (Mammalian orthoreovirus 1) [Reference proteome]
Taxonomic identifier10884 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length721 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-structural protein with ssRNA-binding activity. Is probably involved in the formation of viral inclusions, where the assembly of cores and the replication of viral RNA are thought to occur. Together with mu-2, recruits the other core proteins to these inclusions. Ref.3

Subunit structure

Interacts with mu-2, sigma-NS, inner capsid proteins lambda-1 and sigma-2, and outer capsid protein lambda-2; in viral inclusions. Ref.2 Ref.3

Post-translational modification

The N-terminus is blocked By similarity.

Sequence similarities

Belongs to the orthoreovirus mu-NS protein family.

Ontologies

Keywords
   Coding sequence diversityAlternative initiation
   DomainCoiled coil
   LigandRNA-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform mu-NS (identifier: Q9PY83-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform mu-NSC (identifier: Q9PY83-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.
Note: It is unsure whether Met-41 is the initiator.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 721721Protein mu-NS
PRO_0000345003

Regions

Region1 – 1313Interaction with sigma-NS
Region14 – 4027Interaction with mu-2
Coiled coil523 – 56038 Potential
Coiled coil628 – 68659 Potential

Natural variations

Alternative sequence1 – 4040Missing in isoform mu-NSC.
VSP_034870

Sequences

Sequence LengthMass (Da)Tools
Isoform mu-NS [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: C84FED8A68ED53C9

FASTA72180,166
        10         20         30         40         50         60 
MASFKGFSVN TVPVSKAKRD ISSLAATPGI RSQPFTPSVD MSQSREFLTK AIEQGSMSIP 

        70         80         90        100        110        120 
YQHVNVPKVD RKVVSLVVRP FSSGAFSISG VISPAHAYLL DCLPQLEQAM AFVASPESFQ 

       130        140        150        160        170        180 
ASDVAKRFAI KPGMSLQDAI TAFINFVSAM LKMTVTRQNF DVIVAEIERL ASTSVSVRTE 

       190        200        210        220        230        240 
EAKVADEELM LFGLDHRGPQ QLDISNAKGI MKAADIQATH DVHLAPGVGN IDPEIYNEGR 

       250        260        270        280        290        300 
FMFMQHKPLA ADQSYFTLET ADYFKIYPTY DEHDSRMADQ KQSGLILCTK DEVLAEQTIF 

       310        320        330        340        350        360 
KLDAPDDKTV HLLDRDDDHV VARFTKVFIE DVAPGHHAAQ RSGQRSVLDD LYANTQVVSI 

       370        380        390        400        410        420 
TSAALKWVVK HGVSDGIVNR KNVKVCVGFD PLYTLSTHNG VSLCALLMDE KLSVLNSACR 

       430        440        450        460        470        480 
MTLRSLMKTG RDADAHRAFQ RVLSQGYASL MCYYHPSRKL AYGEVLFLER SSDMVDGIKL 

       490        500        510        520        530        540 
QLDASRQCHE CPVLQQKVVE LEKQIIMQKS IQSDPTPMAL QPLLSQLREL SSEVTRLQME 

       550        560        570        580        590        600 
LSRTQSLNAQ LEADAKSAQA CSLDMYLRHH TCINGHTKED ELLDAVRVAP DVRKEIMEKR 

       610        620        630        640        650        660 
GEVRRGWCER ISKEAAAKCQ TVIDDLTQMN GKQAREITEL RESAENYEKQ IAELVGTITQ 

       670        680        690        700        710        720 
NQMTYQQELQ ALVAKNVELD TMNQRQAKSL RITPSLLSAT PIDSVDGAAD LIDFSVPTDE 


L 

« Hide

Isoform mu-NSC [UniParc].

Checksum: 5EC190C3BFCF6F2E
Show »

FASTA68175,989

References

[1]"Mammalian reovirus M3 gene sequences and conservation of coiled-coil motifs near the carboxyl terminus of the microNS protein."
McCutcheon A.M., Broering T.J., Nibert M.L.
Virology 264:16-24(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], ALTERNATIVE INITIATION.
[2]"Reovirus sigma NS protein localizes to inclusions through an association requiring the mu NS amino terminus."
Miller C.L., Broering T.J., Parker J.S.L., Arnold M.M., Nibert M.L.
J. Virol. 77:4566-4576(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PROTEIN SIGMA-NS AND PROTEIN MU-2.
[3]"Reovirus nonstructural protein mu NS recruits viral core surface proteins and entering core particles to factory-like inclusions."
Broering T.J., Kim J., Miller C.L., Piggott C.D., Dinoso J.B., Nibert M.L., Parker J.S.L.
J. Virol. 78:1882-1892(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PROTEIN LAMBDA-1; PROTEIN LAMBDA-2 AND PROTEIN SIGMA-2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF174382 mRNA. Translation: AAF13169.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameMUNS_REOVL
AccessionPrimary (citable) accession number: Q9PY83
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: May 1, 2000
Last modified: April 3, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families